Restriction map for
pFN21AB7237
(Circular) (Six-base) MAPSORT of: pFN21AB7237.seq Check: 3884 from: 1 to: 6148
pFN21AB7237 6148 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3958 278
Size: 53 83 186 936 2422 2468
Fragments arranged by size:
2468 2422 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2210 2400 3491 1937
Size: 273 190 1091 4594
Fragments arranged by size:
4594 1091 273 190
Acc65I G'GTAC_C
Cuts at: 3469 3469
Size: 6148
AclI AA'CG_TT
Cuts at: 1563 4275 4648 1563
Size: 2712 373 3063
Fragments arranged by size:
3063 2712 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2149 2330 4211 5595 5863 1632
Size: 517 181 1881 1384 268 1917
Fragments arranged by size:
1917 1881 1384 517 268 181
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5951
AflIII A'CryG_T
Cuts at: 1203 3428 5047 6024 1203
Size: 2225 1619 977 1327
Fragments arranged by size:
2225 1619 1327 977
AgeI A'CCGG_T
Cuts at: 4979 4979
Size: 6148
AhdI GACnn_n'nnGTC
Cuts at: 4877 4877
Size: 6148
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6148
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6148
AlwNI CAG_nnn'CTG
Cuts at: 3891 5463 3891
Size: 1572 4576
ApaLI G'TGCA_C
Cuts at: 2480 4205 5361 2480
Size: 1725 1156 3267
Fragments arranged by size:
3267 1725 1156
ApoI r'AATT_y
Cuts at: 1493 2135 2802 3457 3683 3835 5944 1493
Size: 642 667 655 226 152 2109 1697
Fragments arranged by size:
2109 1697 667 655 642 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4702 160
Size: 872 3670 1606
Fragments arranged by size:
3670 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6148
AvaI C'yCGr_G
Cuts at: 1870 1943 3473 1870
Size: 73 1530 4545
Fragments arranged by size:
4545 1530 73
BamHI G'GATC_C
Cuts at: 3478 3845 3478
Size: 367 5781
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3469 4924 618
Size: 325 217 627 1682 1455 1842
Fragments arranged by size:
1842 1682 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2554 2730 3290 3467 1391
Size: 1163 176 560 177 4072
Fragments arranged by size:
4072 1163 560 177 176
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3025 928
Size: 971 1126 4051
Fragments arranged by size:
4051 1126 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4339 4373 1903
Size: 34 2402 34 3678
Fragments arranged by size:
3678 2402 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2306 3040 4040 5250 2306
Size: 734 1000 1210 3204
Fragments arranged by size:
3204 1210 1000 734
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6148
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4759 136
Size: 107 122 71 631 605 3087 1525
Fragments arranged by size:
3087 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6143 6143
Size: 6148
BlpI GC'TnA_GC
Cuts at: 2235 3565 2235
Size: 1330 4818
Bme1580I G_kGCm'C
Cuts at: 1790 2484 4209 5365 1790
Size: 694 1725 1156 2573
Fragments arranged by size:
2573 1725 1156 694
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6148
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3879 4837 442
Size: 416 53 83 399 24 2462 958 1753
Fragments arranged by size:
2462 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6148
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2425 2620 4808 1239
Size: 1186 195 2188 2579
Fragments arranged by size:
2579 2188 1186 195
Bpu10I CC'TnA_GC
Cuts at: 2545 2545
Size: 6148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4273 5138 5436 4273
Size: 865 298 4985
Fragments arranged by size:
4985 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2186 4811 882
Size: 617 687 2625 2219
Fragments arranged by size:
2625 2219 687 617
BsaAI yAC'GTr
Cuts at: 493 1204 3939 493
Size: 711 2735 2702
Fragments arranged by size:
2735 2702 711
BsaBI GATnn'nnATC
Cuts at: 2748 6142 2748
Size: 3394 2754
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3955 4337 4972 275
Size: 53 83 186 936 2422 382 635 1451
Fragments arranged by size:
2422 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4581 4979 5253 5400 5811 4581
Size: 398 274 147 411 4918
Fragments arranged by size:
4918 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2398 2428 1178
Size: 30 165 30 995 30 4898
Fragments arranged by size:
4898 995 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6148
BseYI C'CCAG_C
Cuts at: 2621 2965 5351 5847 2621
Size: 344 2386 496 2922
Fragments arranged by size:
2922 2386 496 344
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5575
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3598 4359 5387 1540
Size: 275 186 1597 761 1028 2301
Fragments arranged by size:
2301 1597 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2053 2484 3290 4209 4294 5365 2053
Size: 431 806 919 85 1071 2836
Fragments arranged by size:
2836 1071 919 806 431 85
BsmI GAATG_Cn'
Cuts at: 2718 3659 3752 2718
Size: 941 93 5114
Fragments arranged by size:
5114 941 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2053 2484 2554 2730 3290 3467 4209
Size: 399 263 431 70 176 560 177 742
Cuts at: 4209 4294 5365 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 560 431 399 263 177
176 85 70
BspEI T'CCGG_A
Cuts at: 5811 5811
Size: 6148
BspHI T'CATG_A
Cuts at: 4037 5767 4037
Size: 1730 4418
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3366 3503 844
Size: 2522 137 3489
Fragments arranged by size:
3489 2522 137
BsrBI CCG'CTC
Cuts at: 1394 1823 4035 1394
Size: 429 2212 3507
Fragments arranged by size:
3507 2212 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4643 4817 65
Size: 1183 3395 174 1396
Fragments arranged by size:
3395 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4792 4979 1700
Size: 110 2982 187 2869
Fragments arranged by size:
2982 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3431 96
Size: 3335 2813
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6148
BssSI C'ACGA_G
Cuts at: 4208 5220 4208
Size: 1012 5136
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6148
BstBI TT'CG_AA
Cuts at: 4952 4952
Size: 6148
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6148
BstYI r'GATC_y
Cuts at: 1972 3321 3478 3845 4230 4247 5699 5785 5797
Size: 1349 157 367 385 17 1452 86 12
Cuts at: 5797 6143 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1349 385 367 346 157 86
17 12
BstZ17I GTA'TAC
Cuts at: 2211 2211
Size: 6148
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5596
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5409
Fragments arranged by size:
5409 683 56
BtsI GCAGTG_nn'
Cuts at: 3305 3664 4458 4478 3305
Size: 359 794 20 4975
Fragments arranged by size:
4975 794 359 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6148
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2664 2699 529
Size: 35 2100 35 3978
Fragments arranged by size:
3978 2100 35 35
DraI TTT'AAA
Cuts at: 2989 3000 3139 3452 2989
Size: 11 139 313 5685
Fragments arranged by size:
5685 313 139 11
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5155 809
Size: 507 3839 1802
Fragments arranged by size:
3839 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3595 4484 6113
Size: 54 1425 50 275 18 1765 889 1629
Cuts at: 6113 8
Size: 43
Fragments arranged by size:
1765 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3595 1537
Size: 275 1783 4090
Fragments arranged by size:
4090 1783 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3162 4078 1353
Size: 1809 916 3423
Fragments arranged by size:
3423 1809 916
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4735 5109 5255 387
Size: 1064 138 3146 374 146 1280
Fragments arranged by size:
3146 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3288 3288
Size: 6148
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2149 2330 2425 2620 4211 4808 5595
Size: 393 517 181 95 195 1591 597 787
Cuts at: 5595 5863 1239
Size: 268 1524
Fragments arranged by size:
1591 1524 787 597 517 393 268 195
181 95
EcoRI G'AATT_C
Cuts at: 2135 2802 3457 3835 2135
Size: 667 655 378 4448
Fragments arranged by size:
4448 667 655 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2179 2935 3431 2179
Size: 756 496 4896
Fragments arranged by size:
4896 756 496
FspI TGC'GCA
Cuts at: 4654 4654
Size: 6148
HaeII r_GCGC'y
Cuts at: 1678 5295 1678
Size: 3617 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3214 3246 3277 3309 4789
Size: 32 135 32 1642 32 31 32 1480
Cuts at: 4789 4821 4863 4895 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1642 1480 135 42 32 32 32
32 32 32 31
HincII GTy'rAC
Cuts at: 669 1657 1938 2591 3492 3738 669
Size: 988 281 653 901 246 3079
Fragments arranged by size:
3079 988 901 653 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6148
HpaI GTT'AAC
Cuts at: 3738 3738
Size: 6148
KpnI G_GTAC'C
Cuts at: 3473 3473
Size: 6148
MfeI C'AATT_G
Cuts at: 3747 3747
Size: 6148
MluI A'CGCG_T
Cuts at: 6024 6024
Size: 6148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2551 5262 5446 1305
Size: 1246 2711 184 2007
Fragments arranged by size:
2711 2007 1246 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4326
Fragments arranged by size:
4326 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2201 2537 2559 4106 4465 4624 518
Size: 648 1035 336 22 1547 359 159 2042
Fragments arranged by size:
2042 1547 1035 648 359 336 159 22
MspA1I CmG'CkG
Cuts at: 1606 3564 4241 5389 5634 1606
Size: 1958 677 1148 245 2120
Fragments arranged by size:
2120 1958 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5596
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6148
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6148
NotI GC'GGCC_GC
Cuts at: 3595 3595
Size: 6148
NspI r_CATG'y
Cuts at: 1132 1229 3312 3432 3506 5051 1132
Size: 97 2083 120 74 1545 2229
Fragments arranged by size:
2229 2083 1545 120 97 74
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6148
PciI A'CATG_T
Cuts at: 3428 5047 3428
Size: 1619 4529
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6148
PmeI GTTT'AAAC
Cuts at: 3452 3452
Size: 6148
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4217 4249 5730 5762 4217
Size: 32 1481 32 4603
Fragments arranged by size:
4603 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6148
PsiI TTA'TAA
Cuts at: 2433 3204 3240 3718 2433
Size: 771 36 478 4863
Fragments arranged by size:
4863 771 478 36
PstI C_TGCA'G
Cuts at: 830 3300 3500 830
Size: 2470 200 3478
Fragments arranged by size:
3478 2470 200
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6148
SacI G_AGCT'C
Cuts at: 3290 3290
Size: 6148
SalI G'TCGA_C
Cuts at: 1936 3490 1936
Size: 1554 4594
SapI GCTCTTCn'nnn_
Cuts at: 3162 3162
Size: 6148
SbfI CC_TGCA'GG
Cuts at: 3500 3500
Size: 6148
ScaI AGT'ACT
Cuts at: 4396 6108 4396
Size: 1712 4436
SfcI C'TryA_G
Cuts at: 826 1045 3296 3496 4631 5312 5503 826
Size: 219 2251 200 1135 681 191 1471
Fragments arranged by size:
2251 1471 1135 681 219 200 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6148
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6148
SmaI CCC'GGG
Cuts at: 1872 3475 1872
Size: 1603 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4252 5153 5415 820
Size: 197 926 2309 901 262 1553
Fragments arranged by size:
2309 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3939 493
Size: 3446 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6148
SphI G_CATG'C
Cuts at: 1132 3506 1132
Size: 2374 3774
SspI AAT'ATT
Cuts at: 5 52 3256 3385 4072 5
Size: 47 3204 129 687 2081
Fragments arranged by size:
3204 2081 687 129 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6148
StyI C'CwwG_G
Cuts at: 513 1065 2627 2730 3173 3587 513
Size: 552 1562 103 443 414 3074
Fragments arranged by size:
3074 1562 552 443 414 103
SwaI ATTT'AAAT
Cuts at: 3139 3139
Size: 6148
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2086 4175 4192 4345 4530 4975 325
Size: 1255 506 2089 17 153 185 445 1498
Fragments arranged by size:
2089 1498 1255 506 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3388 3431 4394
Size: 274 80 33 51 1443 1411 43 963
Cuts at: 4394 6106 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1411 963 274 138 80 51
43 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2398 2430 6108 6140 2398
Size: 32 3678 32 2406
Fragments arranged by size:
3678 2406 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6148
XbaI T'CTAG_A
Cuts at: 3484 3484
Size: 6148
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3040 3040
Size: 6148
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6148
XmaI C'CCGG_G
Cuts at: 1870 3473 1870
Size: 1603 4545
XmnI GAAnn'nnTTC
Cuts at: 3411 4277 3411
Size: 866 5282
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3956 276
Size: 53 83 186 936 2422 2468
Fragments arranged by size:
2468 2422 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI Bsu36I DraIII
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI
SfoI SrfI