Restriction map for
pFN21AB7235
(Circular) (Six-base) MAPSORT of: pFN21AB7235.seq Check: 5691 from: 1 to: 6610
pFN21AB7235 6610 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4420 278
Size: 53 83 186 936 2884 2468
Fragments arranged by size:
2884 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3953 1937
Size: 2016 4594
Acc65I G'GTAC_C
Cuts at: 2985 3931 2985
Size: 946 5664
AclI AA'CG_TT
Cuts at: 1563 4737 5110 1563
Size: 3174 373 3063
Fragments arranged by size:
3174 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2138 2438 2682 2912 4673 6057 6325 1632
Size: 506 300 244 230 1761 1384 268 1917
Fragments arranged by size:
1917 1761 1384 506 300 268 244 230
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6413
AflIII A'CryG_T
Cuts at: 1203 2277 5509 6486 1203
Size: 1074 3232 977 1327
Fragments arranged by size:
3232 1327 1074 977
AgeI A'CCGG_T
Cuts at: 5441 5441
Size: 6610
AhdI GACnn_n'nnGTC
Cuts at: 5339 5339
Size: 6610
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6610
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2986 3573 1184
Size: 1802 587 4221
Fragments arranged by size:
4221 1802 587
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3687 3719 3687
Size: 32 6578
AlwNI CAG_nnn'CTG
Cuts at: 2200 2615 4353 5925 2200
Size: 415 1738 1572 2885
Fragments arranged by size:
2885 1738 1572 415
ApaI G_GGCC'C
Cuts at: 2020 3337 2020
Size: 1317 5293
ApaLI G'TGCA_C
Cuts at: 4667 5823 4667
Size: 1156 5454
ApoI r'AATT_y
Cuts at: 1493 3919 4145 4297 6406 1493
Size: 2426 226 152 2109 1697
Fragments arranged by size:
2426 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5164 160
Size: 872 4132 1606
Fragments arranged by size:
4132 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6610
AvaI C'yCGr_G
Cuts at: 1870 1943 3118 3329 3336 3935 1870
Size: 73 1175 211 7 599 4545
Fragments arranged by size:
4545 1175 599 211 73 7
AvrII C'CTAG_G
Cuts at: 3619 3619
Size: 6610
BamHI G'GATC_C
Cuts at: 2813 3156 3940 4307 2813
Size: 343 784 367 5116
Fragments arranged by size:
5116 784 367 343
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2985 3931 5386 618
Size: 325 217 627 1198 946 1455 1842
Fragments arranged by size:
1842 1455 1198 946 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2020 2115 3146 3337 3431 3656 3929 1391
Size: 629 95 1031 191 94 225 273 4072
Fragments arranged by size:
4072 1031 629 273 225 191 95 94
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5639
BbvCI CC'TCA_GC
Cuts at: 3515 3542 3515
Size: 27 6583
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2262 2296 4801 4835 1903
Size: 34 50 34 241 34 2505 34 3678
Fragments arranged by size:
3678 2505 241 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4502 5712 4502
Size: 1210 5400
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6610
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5221 136
Size: 107 122 71 631 605 3549 1525
Fragments arranged by size:
3549 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3204 6605 3204
Size: 3401 3209
BlpI GC'TnA_GC
Cuts at: 2640 4027 2640
Size: 1387 5223
Bme1580I G_kGCm'C
Cuts at: 1790 2020 2965 3337 3827 4671 5827 1790
Size: 230 945 372 490 844 1156 2573
Fragments arranged by size:
2573 1156 945 844 490 372 230
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6610
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2175 2406 4341
Size: 416 53 83 399 24 758 231 1935
Cuts at: 4341 5299 442
Size: 958 1753
Fragments arranged by size:
1935 1753 958 758 416 399 231 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2934 1056
Size: 1878 4732
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2463 2726 2868 3193 3217 3338 3371 3445
Size: 1224 263 142 325 24 121 33 74
Cuts at: 3445 5270 1239
Size: 1825 2579
Fragments arranged by size:
2579 1825 1224 325 263 142 121 74
33 24
Bpu10I CC'TnA_GC
Cuts at: 3515 3542 3650 3515
Size: 27 108 6475
Fragments arranged by size:
6475 108 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4735 5600 5898 4735
Size: 865 298 5447
Fragments arranged by size:
5447 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3257 3449 3461 5273 882
Size: 617 1758 192 12 1812 2219
Fragments arranged by size:
2219 1812 1758 617 192 12
BsaAI yAC'GTr
Cuts at: 493 1204 2278 4401 493
Size: 711 1074 2123 2702
Fragments arranged by size:
2702 2123 1074 711
BsaBI GATnn'nnATC
Cuts at: 6604 6604
Size: 6610
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4417 4799 5434 275
Size: 53 83 186 936 2884 382 635 1451
Fragments arranged by size:
2884 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5043 5441 5715 5862 6273 5043
Size: 398 274 147 411 5380
Fragments arranged by size:
5380 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2841 2871 3128 3158 3687
Size: 30 165 30 1438 30 257 30 529
Cuts at: 3687 3717 1178
Size: 30 4071
Fragments arranged by size:
4071 1438 529 257 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 2483 3354 1186
Size: 847 450 871 4442
Fragments arranged by size:
4442 871 847 450
BseYI C'CCAG_C
Cuts at: 2463 3508 3698 5813 6309 2463
Size: 1045 190 2115 496 2764
Fragments arranged by size:
2764 2115 1045 496 190
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2309 3543 3593 1163
Size: 573 573 1234 50 4180
Fragments arranged by size:
4180 1234 573 573 50
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4060 4821 5849 1540
Size: 275 186 2059 761 1028 2301
Fragments arranged by size:
2301 2059 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2641 3431 4671 4756 5827 2641
Size: 790 1240 85 1071 3424
Fragments arranged by size:
3424 1240 1071 790 85
BsmI GAATG_Cn'
Cuts at: 4121 4214 4121
Size: 93 6517
BsmBI CGTCTCn'nnnn_
Cuts at: 2315 2315
Size: 6610
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2020 2115 2641 2965 3146 3337 3431
Size: 399 230 95 526 324 181 191 94
Cuts at: 3431 3656 3827 3929 4671 4756 5827 1391
Size: 225 171 102 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 526 399 324 230 225
191 181 171 102 95 94 85
BspEI T'CCGG_A
Cuts at: 6273 6273
Size: 6610
BspHI T'CATG_A
Cuts at: 4499 6229 4499
Size: 1730 4880
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2588 2850 3615 3965 844
Size: 1744 262 765 350 3489
Fragments arranged by size:
3489 1744 765 350 262
BsrBI CCG'CTC
Cuts at: 1394 1823 3494 4497 1394
Size: 429 1671 1003 3507
Fragments arranged by size:
3507 1671 1003 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2879 3864 5105 5279 65
Size: 1183 1631 985 1241 174 1396
Fragments arranged by size:
1631 1396 1241 1183 985 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2401 5254 5441 1700
Size: 110 591 2853 187 2869
Fragments arranged by size:
2869 2853 591 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6610
BssHII G'CGCG_C
Cuts at: 1925 3388 1925
Size: 1463 5147
BssSI C'ACGA_G
Cuts at: 3430 4670 5682 3430
Size: 1240 1012 4358
Fragments arranged by size:
4358 1240 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3505 794
Size: 2711 3899
BstBI TT'CG_AA
Cuts at: 5414 5414
Size: 6610
BstEII G'GTnAC_C
Cuts at: 1184 2029 3106 3487 1184
Size: 845 1077 381 4307
Fragments arranged by size:
4307 1077 845 381
BstXI CCAn_nnnn'nTGG
Cuts at: 3249 3249
Size: 6610
BstYI r'GATC_y
Cuts at: 1972 2527 2790 2813 3156 3204 3477 3940 4307
Size: 555 263 23 343 48 273 463 367
Cuts at: 4307 4692 4709 6161 6247 6259 6605 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 555 463 385 367 346 343
273 263 86 48 23 17 12
Bsu36I CC'TnA_GG
Cuts at: 2498 3632 2498
Size: 1134 5476
BtgI C'CryG_G
Cuts at: 513 1065 2213 2427 2550 3831 513
Size: 552 1148 214 123 1281 3292
Fragments arranged by size:
3292 1281 1148 552 214 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5871
Fragments arranged by size:
5871 683 56
BtsI GCAGTG_nn'
Cuts at: 2862 3639 4126 4920 4940 2862
Size: 777 487 794 20 4532
Fragments arranged by size:
4532 794 777 487 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6610
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3432 3467 3847 3882 529
Size: 35 2868 35 380 35 3257
Fragments arranged by size:
3257 2868 380 35 35 35
DraI TTT'AAA
Cuts at: 3914 3914
Size: 6610
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5617 809
Size: 507 4301 1802
Fragments arranged by size:
4301 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4057 4946 6575
Size: 54 1425 50 275 18 2227 889 1629
Cuts at: 6575 8
Size: 43
Fragments arranged by size:
2227 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4057 1537
Size: 275 2245 4090
Fragments arranged by size:
4090 2245 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2027 2163 2318 3003 3009 4540 1353
Size: 674 136 155 685 6 1531 3423
Fragments arranged by size:
3423 1531 685 674 155 136 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5197 5571 5717 387
Size: 1064 138 3608 374 146 1280
Fragments arranged by size:
3608 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3429 3429
Size: 6610
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2138 2438 2463 2682 2726 2868 2912
Size: 393 506 300 25 219 44 142 44
Cuts at: 2912 3193 3217 3338 3371 3445 4673 5270 6057
Size: 281 24 121 33 74 1228 597 787
Cuts at: 6057 6325 1239
Size: 268 1524
Fragments arranged by size:
1524 1228 787 597 506 393 300 281
268 219 142 121 74 44 44 33
25 24
EcoNI CCTnn'n_nnAGG
Cuts at: 3101 3730 3101
Size: 629 5981
EcoO109I rG'GnC_Cy
Cuts at: 2017 2143 3333 3345 3419 3616 3846 2017
Size: 126 1190 12 74 197 230 4781
Fragments arranged by size:
4781 1190 230 197 126 74 12
EcoRI G'AATT_C
Cuts at: 3919 4297 3919
Size: 378 6232
FspI TGC'GCA
Cuts at: 2774 5116 2774
Size: 2342 4268
HaeII r_GCGC'y
Cuts at: 1678 2180 3522 5757 1678
Size: 502 1342 2235 2531
Fragments arranged by size:
2531 2235 1342 502
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2350 2382 2777 2809 3256
Size: 32 135 32 778 32 395 32 447
Cuts at: 3256 3288 3830 3862 5251 5283 5325 5357 1373
Size: 32 542 32 1389 32 42 32 2626
Fragments arranged by size:
2626 1389 778 542 447 395 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3954 4200 669
Size: 988 281 2016 246 3079
Fragments arranged by size:
3079 2016 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2694 748
Size: 1946 4664
HpaI GTT'AAC
Cuts at: 4200 4200
Size: 6610
KpnI G_GTAC'C
Cuts at: 2989 3935 2989
Size: 946 5664
MfeI C'AATT_G
Cuts at: 4209 4209
Size: 6610
MluI A'CGCG_T
Cuts at: 6486 6486
Size: 6610
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3417 5724 5908 1305
Size: 2112 2307 184 2007
Fragments arranged by size:
2307 2112 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4788
Fragments arranged by size:
4788 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3098 4568 4927 5086 518
Size: 648 1932 1470 359 159 2042
Fragments arranged by size:
2042 1932 1470 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2197 2215 2323 2463 2563 2770 3385 4026
Size: 591 18 108 140 100 207 615 641
Cuts at: 4026 4703 5851 6096 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 641 615 591 245 207
140 108 100 18
NaeI GCC'GGC
Cuts at: 2403 2403
Size: 6610
NcoI C'CATG_G
Cuts at: 513 1065 2427 3831 513
Size: 552 1362 1404 3292
Fragments arranged by size:
3292 1404 1362 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6610
NgoMIV G'CCGG_C
Cuts at: 2401 2401
Size: 6610
NheI G'CTAG_C
Cuts at: 1052 2930 1052
Size: 1878 4732
NotI GC'GGCC_GC
Cuts at: 4057 4057
Size: 6610
NspI r_CATG'y
Cuts at: 1132 1229 2257 3584 3968 5513 1132
Size: 97 1028 1327 384 1545 2229
Fragments arranged by size:
2229 1545 1327 1028 384 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6610
PciI A'CATG_T
Cuts at: 5509 5509
Size: 6610
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6610
PfoI T'CCnGG_A
Cuts at: 3028 3028
Size: 6610
PmeI GTTT'AAAC
Cuts at: 3914 3914
Size: 6610
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3687 3719 4679 4711 6192 6224 3687
Size: 32 960 32 1481 32 4073
Fragments arranged by size:
4073 1481 960 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2143 3345 3419 3616 3846 2143
Size: 1202 74 197 230 4907
Fragments arranged by size:
4907 1202 230 197 74
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6610
PsiI TTA'TAA
Cuts at: 4180 4180
Size: 6610
PspOMI G'GGCC_C
Cuts at: 2016 3333 2016
Size: 1317 5293
PstI C_TGCA'G
Cuts at: 830 2598 2769 3646 3962 830
Size: 1768 171 877 316 3478
Fragments arranged by size:
3478 1768 877 316 171
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6610
PvuII CAG'CTG
Cuts at: 2197 2463 2563 2770 3385 2197
Size: 266 100 207 615 5422
Fragments arranged by size:
5422 615 266 207 100
SacI G_AGCT'C
Cuts at: 3431 3431
Size: 6610
SacII CC_GC'GG
Cuts at: 2216 2216
Size: 6610
SalI G'TCGA_C
Cuts at: 1936 3952 1936
Size: 2016 4594
SanDI GG'GwC_CC
Cuts at: 3345 3345
Size: 6610
SbfI CC_TGCA'GG
Cuts at: 3962 3962
Size: 6610
ScaI AGT'ACT
Cuts at: 4858 6570 4858
Size: 1712 4898
SfcI C'TryA_G
Cuts at: 826 1045 2594 2765 3035 3565 3642 3958 5093
Size: 219 1549 171 270 530 77 316 1135
Cuts at: 5093 5774 5965 826
Size: 681 191 1471
Fragments arranged by size:
1549 1471 1135 681 530 316 270 219
191 171 77
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6610
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6610
SmaI CCC'GGG
Cuts at: 1872 3120 3937 1872
Size: 1248 817 4545
Fragments arranged by size:
4545 1248 817
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4714 5615 5877 820
Size: 197 926 2771 901 262 1553
Fragments arranged by size:
2771 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4401 493
Size: 3908 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6610
SphI G_CATG'C
Cuts at: 1132 2257 3584 3968 1132
Size: 1125 1327 384 3774
Fragments arranged by size:
3774 1327 1125 384
SspI AAT'ATT
Cuts at: 5 52 4534 5
Size: 47 4482 2081
Fragments arranged by size:
4482 2081 47
StuI AGG'CCT
Cuts at: 1517 2397 2602 1517
Size: 880 205 5525
Fragments arranged by size:
5525 880 205
StyI C'CwwG_G
Cuts at: 513 1065 2427 2883 2938 3415 3483 3619 3660
Size: 552 1362 456 55 477 68 136 41
Cuts at: 3660 3831 4049 513
Size: 171 218 3074
Fragments arranged by size:
3074 1362 552 477 456 218 171 136
68 55 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2323 2420 3255 3844 3875 4637 4654
Size: 1255 743 97 835 589 31 762 17
Cuts at: 4654 4807 4992 5437 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 835 762 743 589 445 185
153 97 31 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4856 6568 96
Size: 274 80 33 51 1443 2879 1712 138
Fragments arranged by size:
2879 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2841 2873 3215 3230 3247 3262 6570 6602 2841
Size: 32 342 15 17 15 3308 32 2849
Fragments arranged by size:
3308 2849 342 32 32 17 15 15
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6610
XbaI T'CTAG_A
Cuts at: 3946 3946
Size: 6610
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3192 3217 3894 3192
Size: 25 677 5908
Fragments arranged by size:
5908 677 25
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6610
XmaI C'CCGG_G
Cuts at: 1870 3118 3935 1870
Size: 1248 817 4545
Fragments arranged by size:
4545 1248 817
XmnI GAAnn'nnTTC
Cuts at: 2123 2345 4739 2123
Size: 222 2394 3994
Fragments arranged by size:
3994 2394 222
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4418 276
Size: 53 83 186 936 2884 2468
Fragments arranged by size:
2884 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI BaeI BbeI BfrBI BplI BsiWI
BstZ17I DraIII EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PmlI PspXI PsrI RsrII SapI
SexAI SfiI SfoI SrfI SwaI