Restriction map for
pFN21AB7234
(Circular) (Six-base) MAPSORT of: pFN21AB7234.seq Check: 2048 from: 1 to: 5869
pFN21AB7234 5869 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2141 2141
Size: 5869
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2963 3679 278
Size: 53 83 186 936 1427 716 2468
Fragments arranged by size:
2468 1427 936 716 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2928 3212 1937
Size: 991 284 4594
Fragments arranged by size:
4594 991 284
Acc65I G'GTAC_C
Cuts at: 2478 3190 2478
Size: 712 5157
AclI AA'CG_TT
Cuts at: 1563 3996 4369 1563
Size: 2433 373 3063
Fragments arranged by size:
3063 2433 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3932 5316 5584 1632
Size: 2300 1384 268 1917
Fragments arranged by size:
2300 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5672
AflIII A'CryG_T
Cuts at: 1203 2735 4768 5745 1203
Size: 1532 2033 977 1327
Fragments arranged by size:
2033 1532 1327 977
AgeI A'CCGG_T
Cuts at: 4700 4700
Size: 5869
AhdI GACnn_n'nnGTC
Cuts at: 4598 4598
Size: 5869
AleI CACnn'nnGTG
Cuts at: 1166 2726 1166
Size: 1560 4309
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2916 1184
Size: 1732 4137
AlwNI CAG_nnn'CTG
Cuts at: 2108 2620 3612 5184 2108
Size: 512 992 1572 2793
Fragments arranged by size:
2793 1572 992 512
ApaI G_GGCC'C
Cuts at: 2017 2017
Size: 5869
ApaLI G'TGCA_C
Cuts at: 3926 5082 3926
Size: 1156 4713
ApoI r'AATT_y
Cuts at: 1493 3158 3178 3404 3556 5665 1493
Size: 1665 20 226 152 2109 1697
Fragments arranged by size:
2109 1697 1665 226 152 20
AseI AT'TA_AT
Cuts at: 160 1032 4423 160
Size: 872 3391 1606
Fragments arranged by size:
3391 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5869
AvaI C'yCGr_G
Cuts at: 1870 1943 3194 1870
Size: 73 1251 4545
Fragments arranged by size:
4545 1251 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2572 2605 2572
Size: 33 5836
BamHI G'GATC_C
Cuts at: 2268 3199 3566 2268
Size: 931 367 4571
Fragments arranged by size:
4571 931 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2207 2478 2864 3190 4645
Size: 325 217 627 420 271 386 326 1455
Cuts at: 4645 618
Size: 1842
Fragments arranged by size:
1842 1455 627 420 386 326 325 271
217
BanII G_rGCy'C
Cuts at: 1391 2017 2433 3188 1391
Size: 626 416 755 4072
Fragments arranged by size:
4072 755 626 416
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2337 928
Size: 971 438 4460
Fragments arranged by size:
4460 971 438
BbvCI CC'TCA_GC
Cuts at: 2109 2109
Size: 5869
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2921 2955 4060 4094 1903
Size: 34 984 34 1105 34 3678
Fragments arranged by size:
3678 1105 984 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2860 3761 4971 2860
Size: 901 1210 3758
Fragments arranged by size:
3758 1210 901
BclI T'GATC_A
Cuts at: 1548 3012 1548
Size: 1464 4405
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4480 136
Size: 107 122 71 631 605 2808 1525
Fragments arranged by size:
2808 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5864 5864
Size: 5869
BlpI GC'TnA_GC
Cuts at: 2432 3286 2432
Size: 854 5015
Bme1580I G_kGCm'C
Cuts at: 1790 2017 3930 5086 1790
Size: 227 1913 1156 2573
Fragments arranged by size:
2573 1913 1156 227
BmgBI CAC'GTC
Cuts at: 1318 2736 1318
Size: 1418 4451
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3600 4558 442
Size: 416 53 83 399 24 2183 958 1753
Fragments arranged by size:
2183 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5869
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2266 2846 2266
Size: 580 5289
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2312 2557 4529 1239
Size: 1073 245 1972 2579
Fragments arranged by size:
2579 1972 1073 245
Bpu10I CC'TnA_GC
Cuts at: 2109 2109
Size: 5869
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2261 3994 4859 5157 2261
Size: 1733 865 298 2973
Fragments arranged by size:
2973 1733 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4532 882
Size: 617 3033 2219
Fragments arranged by size:
3033 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2653 2917 3660 493
Size: 711 1449 264 743 2702
Fragments arranged by size:
2702 1449 743 711 264
BsaBI GATnn'nnATC
Cuts at: 5863 5863
Size: 5869
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2882 2960 3676 4058
Size: 53 83 186 936 1349 78 716 382
Cuts at: 4058 4693 275
Size: 635 1451
Fragments arranged by size:
1451 1349 936 716 635 382 186 83
78 53
BsaWI w'CCGG_w
Cuts at: 2627 4302 4700 4974 5121 5532 2627
Size: 1675 398 274 147 411 2964
Fragments arranged by size:
2964 1675 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5644
Fragments arranged by size:
5644 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2258 1186
Size: 1072 4797
BseYI C'CCAG_C
Cuts at: 2179 5072 5568 2179
Size: 2893 496 2480
Fragments arranged by size:
2893 2480 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2126 2684 1163
Size: 573 390 558 4348
Fragments arranged by size:
4348 573 558 390
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2055 3319 4080 5108 1540
Size: 275 186 54 1264 761 1028 2301
Fragments arranged by size:
2301 1264 1028 761 275 186 54
BsiHKAI G_wGCw'C
Cuts at: 2433 3047 3930 4015 5086 2433
Size: 614 883 85 1071 3216
Fragments arranged by size:
3216 1071 883 614 85
BsmI GAATG_Cn'
Cuts at: 3380 3473 3380
Size: 93 5776
BsmBI CGTCTCn'nnnn_
Cuts at: 2819 2819
Size: 5869
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2017 2433 3047 3188 3930 4015 5086
Size: 399 227 416 614 141 742 85 1071
Cuts at: 5086 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 614 416 399 227 141
85
BspEI T'CCGG_A
Cuts at: 2627 5532 2627
Size: 2905 2964
BspHI T'CATG_A
Cuts at: 3758 5488 3758
Size: 1730 4139
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2141 3224 844
Size: 1297 1083 3489
Fragments arranged by size:
3489 1297 1083
BsrBI CCG'CTC
Cuts at: 1394 1823 3756 1394
Size: 429 1933 3507
Fragments arranged by size:
3507 1933 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4364 4538 65
Size: 1183 3116 174 1396
Fragments arranged by size:
3116 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4513 4700 1700
Size: 110 2703 187 2869
Fragments arranged by size:
2869 2703 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5869
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5869
BssSI C'ACGA_G
Cuts at: 3929 4941 3929
Size: 1012 4857
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2653 794
Size: 1859 4010
BstBI TT'CG_AA
Cuts at: 4673 4673
Size: 5869
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5869
BstXI CCAn_nnnn'nTGG
Cuts at: 2153 2525 3091 2153
Size: 372 566 4931
Fragments arranged by size:
4931 566 372
BstYI r'GATC_y
Cuts at: 1972 2268 2769 3199 3566 3951 3968 5420 5506
Size: 296 501 430 367 385 17 1452 86
Cuts at: 5506 5518 5864 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 501 430 385 367 346 296
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2055 2447 2676 2782 2817 2853
Size: 552 939 51 392 229 106 35 36
Cuts at: 2853 513
Size: 3529
Fragments arranged by size:
3529 939 552 392 229 106 51 36
35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5130
Fragments arranged by size:
5130 683 56
BtsI GCAGTG_nn'
Cuts at: 3385 4179 4199 3385
Size: 794 20 5055
Fragments arranged by size:
5055 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5869
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5834
DraI TTT'AAA
Cuts at: 3173 3173
Size: 5869
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4876 809
Size: 507 3560 1802
Fragments arranged by size:
3560 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2030 2052 2896
Size: 54 1425 50 275 18 200 22 844
Cuts at: 2896 3316 4205 5834 8
Size: 420 889 1629 43
Fragments arranged by size:
1629 1425 889 844 420 275 200 54
50 43 22 18
EagI C'GGCC_G
Cuts at: 1537 1812 2052 3316 1537
Size: 275 240 1264 4090
Fragments arranged by size:
4090 1264 275 240
EarI CTCTTCn'nnn_
Cuts at: 1353 2201 3799 1353
Size: 848 1598 3423
Fragments arranged by size:
3423 1598 848
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2825 4456 4830 4976 387
Size: 1064 138 1236 1631 374 146 1280
Fragments arranged by size:
1631 1280 1236 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2431 2431
Size: 5869
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2312 2557 3932 4529 5316 5584 1239
Size: 393 680 245 1375 597 787 268 1524
Fragments arranged by size:
1524 1375 787 680 597 393 268 245
EcoRI G'AATT_C
Cuts at: 3158 3178 3556 3158
Size: 20 378 5471
Fragments arranged by size:
5471 378 20
FspI TGC'GCA
Cuts at: 4375 4375
Size: 5869
HaeII r_GCGC'y
Cuts at: 1678 2023 2621 2912 2999 5016 1678
Size: 345 598 291 87 2017 2531
Fragments arranged by size:
2531 2017 598 345 291 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4510 4542 4584 4616 1373
Size: 32 135 32 2938 32 42 32 2626
Fragments arranged by size:
2938 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2733 3213 3459 669
Size: 988 281 795 480 246 3079
Fragments arranged by size:
3079 988 795 480 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5869
HpaI GTT'AAC
Cuts at: 3459 3459
Size: 5869
KpnI G_GTAC'C
Cuts at: 2482 3194 2482
Size: 712 5157
MfeI C'AATT_G
Cuts at: 3468 3468
Size: 5869
MluI A'CGCG_T
Cuts at: 5745 5745
Size: 5869
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4983 5167 1305
Size: 3678 184 2007
Fragments arranged by size:
3678 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4047
Fragments arranged by size:
4047 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2148 2726 2898 3020 3827 4186 4345
Size: 648 982 578 172 122 807 359 159
Cuts at: 4345 518
Size: 2042
Fragments arranged by size:
2042 982 807 648 578 359 172 159
122
MspA1I CmG'CkG
Cuts at: 1606 2035 2057 2436 2449 2775 2784 2855 3285
Size: 429 22 379 13 326 9 71 430
Cuts at: 3285 3962 5110 5355 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 430 429 379 326 245
71 22 13 9
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4378
Fragments arranged by size:
4378 939 552
NdeI CA'TA_TG
Cuts at: 387 3017 387
Size: 2630 3239
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5869
NotI GC'GGCC_GC
Cuts at: 2052 3316 2052
Size: 1264 4605
NspI r_CATG'y
Cuts at: 1132 1229 3227 4772 1132
Size: 97 1998 1545 2229
Fragments arranged by size:
2229 1998 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5869
PciI A'CATG_T
Cuts at: 4768 4768
Size: 5869
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2704 1108
Size: 1596 4273
PfoI T'CCnGG_A
Cuts at: 2397 2397
Size: 5869
PmeI GTTT'AAAC
Cuts at: 3173 3173
Size: 5869
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3938 3970 5451 5483 3938
Size: 32 1481 32 4324
Fragments arranged by size:
4324 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5869
PsiI TTA'TAA
Cuts at: 3439 3439
Size: 5869
PspOMI G'GGCC_C
Cuts at: 2013 2013
Size: 5869
PstI C_TGCA'G
Cuts at: 830 3221 830
Size: 2391 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5869
PvuII CAG'CTG
Cuts at: 2436 2436
Size: 5869
SacI G_AGCT'C
Cuts at: 2433 2433
Size: 5869
SacII CC_GC'GG
Cuts at: 2058 2450 2785 2856 2058
Size: 392 335 71 5071
Fragments arranged by size:
5071 392 335 71
SalI G'TCGA_C
Cuts at: 1936 3211 1936
Size: 1275 4594
SbfI CC_TGCA'GG
Cuts at: 3221 3221
Size: 5869
ScaI AGT'ACT
Cuts at: 2669 4117 5829 2669
Size: 1448 1712 2709
Fragments arranged by size:
2709 1712 1448
SfcI C'TryA_G
Cuts at: 826 1045 3217 4352 5033 5224 826
Size: 219 2172 1135 681 191 1471
Fragments arranged by size:
2172 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5869
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5869
SmaI CCC'GGG
Cuts at: 1872 3196 1872
Size: 1324 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2240 3973 4874 5136 820
Size: 197 926 297 1733 901 262 1553
Fragments arranged by size:
1733 1553 926 901 297 262 197
SnaBI TAC'GTA
Cuts at: 493 2917 3660 493
Size: 2424 743 2702
Fragments arranged by size:
2702 2424 743
SpeI A'CTAG_T
Cuts at: 152 2365 152
Size: 2213 3656
SphI G_CATG'C
Cuts at: 1132 3227 1132
Size: 2095 3774
SspI AAT'ATT
Cuts at: 5 52 3793 5
Size: 47 3741 2081
Fragments arranged by size:
3741 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5869
StyI C'CwwG_G
Cuts at: 513 1065 2004 3308 513
Size: 552 939 1304 3074
Fragments arranged by size:
3074 1304 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2351 3896 3913 4066 4251 4696 325
Size: 1255 771 1545 17 153 185 445 1498
Fragments arranged by size:
1545 1498 1255 771 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2083 2667 4115
Size: 274 80 33 51 1443 106 584 1448
Cuts at: 4115 5827 96
Size: 1712 138
Fragments arranged by size:
1712 1448 1443 584 274 138 106 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5829 5861 5829
Size: 32 5837
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5869
XbaI T'CTAG_A
Cuts at: 3205 3205
Size: 5869
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3083 3083
Size: 5869
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5869
XmaI C'CCGG_G
Cuts at: 1870 3194 1870
Size: 1324 4545
XmnI GAAnn'nnTTC
Cuts at: 3998 3998
Size: 5869
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2961 3677 276
Size: 53 83 186 936 1427 716 2468
Fragments arranged by size:
2468 1427 936 716 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BfrBI BsiWI BstZ17I
Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspXI PsrI RsrII SanDI SapI SexAI SfiI
SfoI SrfI SwaI