Restriction map for
pFN21AB7233
(Circular) (Six-base) MAPSORT of: pFN21AB7233.seq Check: 9905 from: 1 to: 6052
pFN21AB7233 6052 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3862 278
Size: 53 83 186 936 2326 2468
Fragments arranged by size:
2468 2326 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2180 3395 1937
Size: 243 1215 4594
Fragments arranged by size:
4594 1215 243
Acc65I G'GTAC_C
Cuts at: 3373 3373
Size: 6052
AclI AA'CG_TT
Cuts at: 1563 4179 4552 1563
Size: 2616 373 3063
Fragments arranged by size:
3063 2616 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2717 2922 4115 5499 5767 1632
Size: 1085 205 1193 1384 268 1917
Fragments arranged by size:
1917 1384 1193 1085 268 205
AfeI AGC'GCT
Cuts at: 2594 2594
Size: 6052
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5855
AflIII A'CryG_T
Cuts at: 1203 4951 5928 1203
Size: 3748 977 1327
Fragments arranged by size:
3748 1327 977
AgeI A'CCGG_T
Cuts at: 3311 4883 3311
Size: 1572 4480
AhdI GACnn_n'nnGTC
Cuts at: 2458 4781 2458
Size: 2323 3729
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6052
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2406 2930 1184
Size: 1222 524 4306
Fragments arranged by size:
4306 1222 524
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2870 2871 2902 2903 2870
Size: 1 31 1 6019
Fragments arranged by size:
6019 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 2205 3172 3795 5367 2205
Size: 967 623 1572 2890
Fragments arranged by size:
2890 1572 967 623
ApaLI G'TGCA_C
Cuts at: 4109 5265 4109
Size: 1156 4896
ApoI r'AATT_y
Cuts at: 1493 3361 3587 3739 5848 1493
Size: 1868 226 152 2109 1697
Fragments arranged by size:
2109 1868 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4606 160
Size: 872 3574 1606
Fragments arranged by size:
3574 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6052
AvaI C'yCGr_G
Cuts at: 1870 1943 2414 3377 1870
Size: 73 471 963 4545
Fragments arranged by size:
4545 963 471 73
BamHI G'GATC_C
Cuts at: 3382 3749 3382
Size: 367 5685
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2097 3373 4828 618
Size: 325 217 627 310 1276 1455 1842
Fragments arranged by size:
1842 1455 1276 627 325 310 217
BanII G_rGCy'C
Cuts at: 1391 2487 2799 2839 3371 1391
Size: 1096 312 40 532 4072
Fragments arranged by size:
4072 1096 532 312 40
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5081
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4243 4277 1903
Size: 34 2306 34 3678
Fragments arranged by size:
3678 2306 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3944 5154 3944
Size: 1210 4842
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6052
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2150 4663 136
Size: 107 122 71 631 605 478 2513 1525
Fragments arranged by size:
2513 1525 631 605 478 122 107 71
BglII A'GATC_T
Cuts at: 6047 6047
Size: 6052
BlpI GC'TnA_GC
Cuts at: 3469 3469
Size: 6052
Bme1580I G_kGCm'C
Cuts at: 1790 2450 4113 5269 1790
Size: 660 1663 1156 2573
Fragments arranged by size:
2573 1663 1156 660
BmgBI CAC'GTC
Cuts at: 1318 3181 1318
Size: 1863 4189
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2890 3783 4741
Size: 416 53 83 399 24 1473 893 958
Cuts at: 4741 442
Size: 1753
Fragments arranged by size:
1753 1473 958 893 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6052
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2728 2728
Size: 6052
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2079 2381 2399 2420 2471 2603 2696 2987
Size: 840 302 18 21 51 132 93 291
Cuts at: 2987 3014 3164 3248 4712 1239
Size: 27 150 84 1464 2579
Fragments arranged by size:
2579 1464 840 302 291 150 132 93
84 51 27 21 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2702 4177 5042 5340 2702
Size: 1475 865 298 3414
Fragments arranged by size:
3414 1475 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2315 2396 2852 3324 4715 882
Size: 617 816 81 456 472 1391 2219
Fragments arranged by size:
2219 1391 816 617 472 456 81
BsaAI yAC'GTr
Cuts at: 493 1204 3843 493
Size: 711 2639 2702
Fragments arranged by size:
2702 2639 711
BsaBI GATnn'nnATC
Cuts at: 6046 6046
Size: 6052
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3859 4241 4876 275
Size: 53 83 186 936 2326 382 635 1451
Fragments arranged by size:
2326 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2775 3216 3311 4485 4883 5157 5304 5715 2775
Size: 441 95 1174 398 274 147 411 3112
Fragments arranged by size:
3112 1174 441 411 398 274 147 95
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2009 2038 2039 2068 2930
Size: 30 165 30 606 29 1 29 862
Cuts at: 2930 2960 1178
Size: 30 4270
Fragments arranged by size:
4270 862 606 165 30 30 30 29
29 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2017 2402 2434 2495 2717 2767 2951 1186
Size: 831 385 32 61 222 50 184 4287
Fragments arranged by size:
4287 831 385 222 184 61 50 32
BseYI C'CCAG_C
Cuts at: 2198 2682 2761 3012 5255 5751 2198
Size: 484 79 251 2243 496 2499
Fragments arranged by size:
2499 2243 496 484 251 79
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2524 1163
Size: 573 788 4691
Fragments arranged by size:
4691 788 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3502 4263 5291 1540
Size: 275 186 1501 761 1028 2301
Fragments arranged by size:
2301 1501 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2487 4113 4198 5269 2487
Size: 1626 85 1071 3270
Fragments arranged by size:
3270 1626 1071 85
BsmI GAATG_Cn'
Cuts at: 2395 2524 2992 3563 3656 2395
Size: 129 468 571 93 4791
Fragments arranged by size:
4791 571 468 129 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2450 2487 2799 2839 3371 4113 4198
Size: 399 660 37 312 40 532 742 85
Cuts at: 4198 5269 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 660 532 399 312 85
40 37
BspEI T'CCGG_A
Cuts at: 2775 5715 2775
Size: 2940 3112
BspHI T'CATG_A
Cuts at: 3941 5671 3941
Size: 1730 4322
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3407 844
Size: 2563 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3148 3939 1394
Size: 429 1325 791 3507
Fragments arranged by size:
3507 1325 791 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4547 4721 65
Size: 1183 3299 174 1396
Fragments arranged by size:
3299 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3311 4696 4883 1700
Size: 110 1501 1385 187 2869
Fragments arranged by size:
2869 1501 1385 187 110
BsrGI T'GTAC_A
Cuts at: 96 2533 96
Size: 2437 3615
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6052
BssSI C'ACGA_G
Cuts at: 2432 2729 4112 5124 2432
Size: 297 1383 1012 3360
Fragments arranged by size:
3360 1383 1012 297
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6052
BstBI TT'CG_AA
Cuts at: 4856 4856
Size: 6052
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6052
BstXI CCAn_nnnn'nTGG
Cuts at: 2359 2359
Size: 6052
BstYI r'GATC_y
Cuts at: 1972 2496 2961 3129 3382 3749 4134 4151 5603
Size: 524 465 168 253 367 385 17 1452
Cuts at: 5603 5689 5701 6047 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 524 465 385 367 346 253
168 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2049 2850 3331 513
Size: 552 984 801 481 3234
Fragments arranged by size:
3234 984 801 552 481
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5313
Fragments arranged by size:
5313 683 56
BtsI GCAGTG_nn'
Cuts at: 2226 2951 3568 4362 4382 2226
Size: 725 617 794 20 3896
Fragments arranged by size:
3896 794 725 617 20
ClaI AT'CG_AT
Cuts at: 1554 2088 1554
Size: 534 5518
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6017
DraI TTT'AAA
Cuts at: 3356 3356
Size: 6052
DraIII CAC_nnn'GTG
Cuts at: 2445 2445
Size: 6052
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5059 809
Size: 507 3743 1802
Fragments arranged by size:
3743 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2142 2308 2349
Size: 54 1425 50 275 18 312 166 41
Cuts at: 2349 2661 3328 3499 4388 6017 8
Size: 312 667 171 889 1629 43
Fragments arranged by size:
1629 1425 889 667 312 312 275 171
166 54 50 43 41 18
EagI C'GGCC_G
Cuts at: 1537 1812 3499 1537
Size: 275 1687 4090
Fragments arranged by size:
4090 1687 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3108 3982 1353
Size: 1755 874 3423
Fragments arranged by size:
3423 1755 874
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2633 4639 5013 5159 387
Size: 1064 138 1044 2006 374 146 1280
Fragments arranged by size:
2006 1280 1064 1044 374 146 138
EcoICRI GAG'CTC
Cuts at: 2485 2485
Size: 6052
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2079 2381 2399 2420 2471 2603 2696
Size: 393 447 302 18 21 51 132 93
Cuts at: 2696 2717 2922 2987 3014 3164 3248 4115 4712
Size: 21 205 65 27 150 84 867 597
Cuts at: 4712 5499 5767 1239
Size: 787 268 1524
Fragments arranged by size:
1524 867 787 597 447 393 302 268
205 150 132 93 84 65 51 27
21 21 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2363 2663 2699 2363
Size: 300 36 5716
Fragments arranged by size:
5716 300 36
EcoO109I rG'GnC_Cy
Cuts at: 2247 2283 2247
Size: 36 6016
EcoRI G'AATT_C
Cuts at: 3361 3739 3361
Size: 378 5674
FspI TGC'GCA
Cuts at: 4558 4558
Size: 6052
HaeII r_GCGC'y
Cuts at: 1678 2596 3007 5199 1678
Size: 918 411 2192 2531
Fragments arranged by size:
2531 2192 918 411
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2444 2476 2821 2853 3107
Size: 32 135 32 872 32 345 32 254
Cuts at: 3107 3139 4693 4725 4767 4799 1373
Size: 32 1554 32 42 32 2626
Fragments arranged by size:
2626 1554 872 345 254 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2181 3396 3642 669
Size: 988 281 243 1215 246 3079
Fragments arranged by size:
3079 1215 988 281 246 243
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6052
HpaI GTT'AAC
Cuts at: 3642 3642
Size: 6052
KpnI G_GTAC'C
Cuts at: 3377 3377
Size: 6052
MfeI C'AATT_G
Cuts at: 3651 3651
Size: 6052
MluI A'CGCG_T
Cuts at: 5928 5928
Size: 6052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2937 3174 5166 5350 1305
Size: 1632 237 1992 184 2007
Fragments arranged by size:
2007 1992 1632 237 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2144 2310 2351 2663 3330
Size: 54 1425 343 312 166 41 312 667
Cuts at: 3330 10
Size: 2732
Fragments arranged by size:
2732 1425 667 343 312 312 166 54
41
MslI CAynn'nnrTG
Cuts at: 518 1166 2871 4010 4369 4528 518
Size: 648 1705 1139 359 159 2042
Fragments arranged by size:
2042 1705 1139 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2377 2608 2761 3468 4145 5293 5538 1606
Size: 771 231 153 707 677 1148 245 2120
Fragments arranged by size:
2120 1148 771 707 677 245 231 153
NcoI C'CATG_G
Cuts at: 513 1065 2049 2850 3331 513
Size: 552 984 801 481 3234
Fragments arranged by size:
3234 984 801 552 481
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6052
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6052
NotI GC'GGCC_GC
Cuts at: 3499 3499
Size: 6052
NspI r_CATG'y
Cuts at: 1132 1229 3410 4955 1132
Size: 97 2181 1545 2229
Fragments arranged by size:
2229 2181 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2188 1348
Size: 840 5212
PciI A'CATG_T
Cuts at: 4951 4951
Size: 6052
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2107 2611 2875 3299 1108
Size: 999 504 264 424 3861
Fragments arranged by size:
3861 999 504 424 264
PfoI T'CCnGG_A
Cuts at: 2056 2643 2964 2056
Size: 587 321 5144
Fragments arranged by size:
5144 587 321
PmeI GTTT'AAAC
Cuts at: 3356 3356
Size: 6052
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4121 4153 5634 5666 4121
Size: 32 1481 32 4507
Fragments arranged by size:
4507 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2247 2247
Size: 6052
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6052
PsiI TTA'TAA
Cuts at: 3622 3622
Size: 6052
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3146 3178 3146
Size: 32 6020
PstI C_TGCA'G
Cuts at: 830 2919 2946 3039 3404 830
Size: 2089 27 93 365 3478
Fragments arranged by size:
3478 2089 365 93 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6052
PvuII CAG'CTG
Cuts at: 2377 2608 2761 2377
Size: 231 153 5668
Fragments arranged by size:
5668 231 153
SacI G_AGCT'C
Cuts at: 2487 2487
Size: 6052
SalI G'TCGA_C
Cuts at: 1936 2179 3394 1936
Size: 243 1215 4594
Fragments arranged by size:
4594 1215 243
SapI GCTCTTCn'nnn_
Cuts at: 3108 3108
Size: 6052
SbfI CC_TGCA'GG
Cuts at: 2919 3404 2919
Size: 485 5567
ScaI AGT'ACT
Cuts at: 4300 6012 4300
Size: 1712 4340
SexAI A'CCwGG_T
Cuts at: 3165 3165
Size: 6052
SfcI C'TryA_G
Cuts at: 826 1045 2017 2915 2942 3035 3400 4535 5216
Size: 219 972 898 27 93 365 1135 681
Cuts at: 5216 5407 826
Size: 191 1471
Fragments arranged by size:
1471 1135 972 898 681 365 219 191
93 27
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6052
SgrAI Cr'CCGG_yG
Cuts at: 1700 3311 1700
Size: 1611 4441
SmaI CCC'GGG
Cuts at: 1872 3379 1872
Size: 1507 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2414 2717 4156 5057 5319 820
Size: 197 926 471 303 1439 901 262 1553
Fragments arranged by size:
1553 1439 926 901 471 303 262 197
SnaBI TAC'GTA
Cuts at: 493 3843 493
Size: 3350 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6052
SphI G_CATG'C
Cuts at: 1132 3410 1132
Size: 2278 3774
SspI AAT'ATT
Cuts at: 5 52 3976 5
Size: 47 3924 2081
Fragments arranged by size:
3924 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6052
StyI C'CwwG_G
Cuts at: 513 1065 2049 2664 2850 3331 3491 513
Size: 552 984 615 186 481 160 3074
Fragments arranged by size:
3074 984 615 552 481 186 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2085 4079 4096 4249 4434 4879 325
Size: 1255 505 1994 17 153 185 445 1498
Fragments arranged by size:
1994 1498 1255 505 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2533 3281 4298
Size: 274 80 33 51 1443 556 748 1017
Cuts at: 4298 6010 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1017 748 556 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2038 2070 2218 2250 2930 2962 6012 6044 2038
Size: 32 148 32 680 32 3050 32 2046
Fragments arranged by size:
3050 2046 680 148 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6052
XbaI T'CTAG_A
Cuts at: 3388 3388
Size: 6052
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2137 2671 2137
Size: 534 5518
XhoI C'TCGA_G
Cuts at: 1943 2414 1943
Size: 471 5581
XmaI C'CCGG_G
Cuts at: 1870 3377 1870
Size: 1507 4545
XmnI GAAnn'nnTTC
Cuts at: 4181 4181
Size: 6052
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3860 276
Size: 53 83 186 936 2326 2468
Fragments arranged by size:
2468 2326 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI RsrII SacII SanDI SfiI SfoI
SrfI SwaI