Restriction map for
pFN21AB7230
(Circular) (Six-base) MAPSORT of: pFN21AB7230.seq Check: 6285 from: 1 to: 5758
pFN21AB7230 5758 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3568 278
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3101 1937
Size: 1164 4594
Acc65I G'GTAC_C
Cuts at: 3079 3079
Size: 5758
AclI AA'CG_TT
Cuts at: 1563 3885 4258 1563
Size: 2322 373 3063
Fragments arranged by size:
3063 2322 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3002 3821 5205 5473 1632
Size: 1370 819 1384 268 1917
Fragments arranged by size:
1917 1384 1370 819 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5561
AflIII A'CryG_T
Cuts at: 1203 2930 4657 5634 1203
Size: 1727 1727 977 1327
Fragments arranged by size:
1727 1727 1327 977
AgeI A'CCGG_T
Cuts at: 4589 4589
Size: 5758
AhdI GACnn_n'nnGTC
Cuts at: 4487 4487
Size: 5758
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5758
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5758
AlwNI CAG_nnn'CTG
Cuts at: 2604 3501 5073 2604
Size: 897 1572 3289
Fragments arranged by size:
3289 1572 897
ApaLI G'TGCA_C
Cuts at: 3815 4971 3815
Size: 1156 4602
ApoI r'AATT_y
Cuts at: 1493 2069 2697 3003 3067 3293 3445 5554 1493
Size: 576 628 306 64 226 152 2109 1697
Fragments arranged by size:
2109 1697 628 576 306 226 152 64
AseI AT'TA_AT
Cuts at: 160 1032 4312 160
Size: 872 3280 1606
Fragments arranged by size:
3280 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
AvaI C'yCGr_G
Cuts at: 1870 1943 3083 1870
Size: 73 1140 4545
Fragments arranged by size:
4545 1140 73
BamHI G'GATC_C
Cuts at: 2159 2857 3088 3455 2159
Size: 698 231 367 4462
Fragments arranged by size:
4462 698 367 231
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3079 4534 618
Size: 325 217 627 1292 1455 1842
Fragments arranged by size:
1842 1455 1292 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3077 1391
Size: 1686 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2533 928
Size: 971 634 4153
Fragments arranged by size:
4153 971 634
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3949 3983 1903
Size: 34 2012 34 3678
Fragments arranged by size:
3678 2012 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2719 3650 4860 2719
Size: 931 1210 3617
Fragments arranged by size:
3617 1210 931
BclI T'GATC_A
Cuts at: 1548 2307 1548
Size: 759 4999
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4369 136
Size: 107 122 71 631 605 2697 1525
Fragments arranged by size:
2697 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2096 2117 5753 2096
Size: 21 3636 2101
Fragments arranged by size:
3636 2101 21
BlpI GC'TnA_GC
Cuts at: 2381 3175 2381
Size: 794 4964
Bme1580I G_kGCm'C
Cuts at: 1790 3819 4975 1790
Size: 2029 1156 2573
Fragments arranged by size:
2573 2029 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5758
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2603 3489 4447
Size: 416 53 83 399 24 1186 886 958
Cuts at: 4447 442
Size: 1753
Fragments arranged by size:
1753 1186 958 886 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5758
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2538 4418 1239
Size: 1299 1880 2579
Fragments arranged by size:
2579 1880 1299
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3883 4748 5046 3883
Size: 865 298 4595
Fragments arranged by size:
4595 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4421 882
Size: 617 2922 2219
Fragments arranged by size:
2922 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3549 493
Size: 711 2345 2702
Fragments arranged by size:
2702 2345 711
BsaBI GATnn'nnATC
Cuts at: 5752 5752
Size: 5758
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3565 3947 4582 275
Size: 53 83 186 936 2032 382 635 1451
Fragments arranged by size:
2032 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4191 4589 4863 5010 5421 4191
Size: 398 274 147 411 4528
Fragments arranged by size:
4528 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5533
Fragments arranged by size:
5533 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5758
BseYI C'CCAG_C
Cuts at: 4961 5457 4961
Size: 496 5262
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5185
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3208 3969 4997 1540
Size: 275 186 1207 761 1028 2301
Fragments arranged by size:
2301 1207 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2980 3819 3904 4975 2980
Size: 839 85 1071 3763
Fragments arranged by size:
3763 1071 839 85
BsmI GAATG_Cn'
Cuts at: 3269 3362 3269
Size: 93 5665
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2980 3077 3819 3904 4975 1391
Size: 399 1190 97 742 85 1071 2174
Fragments arranged by size:
2174 1190 1071 742 399 97 85
BspEI T'CCGG_A
Cuts at: 5421 5421
Size: 5758
BspHI T'CATG_A
Cuts at: 3647 5377 3647
Size: 1730 4028
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2642 3113 844
Size: 1798 471 3489
Fragments arranged by size:
3489 1798 471
BsrBI CCG'CTC
Cuts at: 1394 1823 3645 1394
Size: 429 1822 3507
Fragments arranged by size:
3507 1822 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4253 4427 65
Size: 1183 3005 174 1396
Fragments arranged by size:
3005 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4402 4589 1700
Size: 110 2592 187 2869
Fragments arranged by size:
2869 2592 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5758
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5758
BssSI C'ACGA_G
Cuts at: 3818 4830 3818
Size: 1012 4746
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5758
BstBI TT'CG_AA
Cuts at: 4562 4562
Size: 5758
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5758
BstYI r'GATC_y
Cuts at: 1972 2096 2117 2159 2857 3088 3455 3840 3857
Size: 124 21 42 698 231 367 385 17
Cuts at: 3857 5309 5395 5407 5753 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 698 385 367 346 231 124
86 42 21 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2256 513
Size: 552 939 252 4015
Fragments arranged by size:
4015 939 552 252
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5019
Fragments arranged by size:
5019 683 56
BtsI GCAGTG_nn'
Cuts at: 3274 4068 4088 3274
Size: 794 20 4944
Fragments arranged by size:
4944 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5758
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5723
DraI TTT'AAA
Cuts at: 3062 3062
Size: 5758
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4765 809
Size: 507 3449 1802
Fragments arranged by size:
3449 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2259 2415 3205
Size: 54 1425 50 275 18 429 156 790
Cuts at: 3205 4094 5723 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 790 429 275 156 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3205 1537
Size: 275 1393 4090
Fragments arranged by size:
4090 1393 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3688 1353
Size: 2335 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4345 4719 4865 387
Size: 1064 138 2756 374 146 1280
Fragments arranged by size:
2756 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2538 3002 3821 4418 5205 5473 1239
Size: 393 906 464 819 597 787 268 1524
Fragments arranged by size:
1524 906 819 787 597 464 393 268
EcoRI G'AATT_C
Cuts at: 3067 3445 3067
Size: 378 5380
FspI TGC'GCA
Cuts at: 4264 4264
Size: 5758
HaeII r_GCGC'y
Cuts at: 1678 4905 1678
Size: 3227 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2274 2306 4399 4431 4473
Size: 32 135 32 702 32 2093 32 42
Cuts at: 4473 4505 1373
Size: 32 2626
Fragments arranged by size:
2626 2093 702 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3102 3348 669
Size: 988 281 1164 246 3079
Fragments arranged by size:
3079 1164 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5758
HpaI GTT'AAC
Cuts at: 3348 3348
Size: 5758
KpnI G_GTAC'C
Cuts at: 3083 3083
Size: 5758
MfeI C'AATT_G
Cuts at: 2074 3357 2074
Size: 1283 4475
MluI A'CGCG_T
Cuts at: 5634 5634
Size: 5758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4872 5056 1305
Size: 3567 184 2007
Fragments arranged by size:
3567 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2261 2417 10
Size: 54 1425 343 429 156 3351
Fragments arranged by size:
3351 1425 429 343 156 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2291 2724 3716 4075 4234 518
Size: 648 1125 433 992 359 159 2042
Fragments arranged by size:
2042 1125 992 648 433 359 159
MspA1I CmG'CkG
Cuts at: 1606 3174 3851 4999 5244 1606
Size: 1568 677 1148 245 2120
Fragments arranged by size:
2120 1568 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2256 513
Size: 552 939 252 4015
Fragments arranged by size:
4015 939 552 252
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5758
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5758
NotI GC'GGCC_GC
Cuts at: 3205 3205
Size: 5758
NspI r_CATG'y
Cuts at: 1132 1229 2723 2934 3116 4661 1132
Size: 97 1494 211 182 1545 2229
Fragments arranged by size:
2229 1545 1494 211 182 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5758
PciI A'CATG_T
Cuts at: 2930 4657 2930
Size: 1727 4031
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5758
PmeI GTTT'AAAC
Cuts at: 3062 3062
Size: 5758
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3827 3859 5340 5372 3827
Size: 32 1481 32 4213
Fragments arranged by size:
4213 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5758
PsiI TTA'TAA
Cuts at: 3328 3328
Size: 5758
PstI C_TGCA'G
Cuts at: 830 3110 830
Size: 2280 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5758
SalI G'TCGA_C
Cuts at: 1936 3100 1936
Size: 1164 4594
SbfI CC_TGCA'GG
Cuts at: 3110 3110
Size: 5758
ScaI AGT'ACT
Cuts at: 4006 5718 4006
Size: 1712 4046
SfcI C'TryA_G
Cuts at: 826 1045 2883 3106 4241 4922 5113 826
Size: 219 1838 223 1135 681 191 1471
Fragments arranged by size:
1838 1471 1135 681 223 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5758
SmaI CCC'GGG
Cuts at: 1872 3085 1872
Size: 1213 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3862 4763 5025 820
Size: 197 926 1919 901 262 1553
Fragments arranged by size:
1919 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3549 493
Size: 3056 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5758
SphI G_CATG'C
Cuts at: 1132 3116 1132
Size: 1984 3774
SspI AAT'ATT
Cuts at: 5 52 2178 3682 5
Size: 47 2126 1504 2081
Fragments arranged by size:
2126 2081 1504 47
StuI AGG'CCT
Cuts at: 1517 2552 1517
Size: 1035 4723
StyI C'CwwG_G
Cuts at: 513 1065 2004 2256 3197 513
Size: 552 939 252 941 3074
Fragments arranged by size:
3074 941 939 552 252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2114 2500 3785 3802 3955 4140 4585
Size: 1255 534 386 1285 17 153 185 445
Cuts at: 4585 325
Size: 1498
Fragments arranged by size:
1498 1285 1255 534 445 386 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2985 4004 5716
Size: 274 80 33 51 1443 1008 1019 1712
Cuts at: 5716 96
Size: 138
Fragments arranged by size:
1712 1443 1019 1008 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2920 2952 5718 5750 2920
Size: 32 2766 32 2928
Fragments arranged by size:
2928 2766 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5758
XbaI T'CTAG_A
Cuts at: 3094 3094
Size: 5758
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2644 2644
Size: 5758
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5758
XmaI C'CCGG_G
Cuts at: 1870 3083 1870
Size: 1213 4545
XmnI GAAnn'nnTTC
Cuts at: 3887 3887
Size: 5758
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3566 276
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI