(Circular) (Six-base) MAPSORT of: pFN21AB7226.seq Check: 7573 from: 1 to: 6064 pFN21AB7226 6064 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3874 278 Size: 53 83 186 936 2338 2468 Fragments arranged by size: 2468 2338 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3407 1937 Size: 1470 4594 Acc65I G'GTAC_C Cuts at: 3385 3385 Size: 6064 AclI AA'CG_TT Cuts at: 1563 4191 4564 1563 Size: 2628 373 3063 Fragments arranged by size: 3063 2628 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2653 2922 3110 4127 5511 5779 1632 Size: 1021 269 188 1017 1384 268 1917 Fragments arranged by size: 1917 1384 1021 1017 269 268 188 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5867 AflIII A'CryG_T Cuts at: 1203 4963 5940 1203 Size: 3760 977 1327 Fragments arranged by size: 3760 1327 977 AgeI A'CCGG_T Cuts at: 4895 4895 Size: 6064 AhdI GACnn_n'nnGTC Cuts at: 4793 4793 Size: 6064 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6064 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3011 1184 Size: 1827 4237 AlwNI CAG_nnn'CTG Cuts at: 2773 3807 5379 2773 Size: 1034 1572 3458 Fragments arranged by size: 3458 1572 1034 ApaLI G'TGCA_C Cuts at: 3077 3107 4121 5277 3077 Size: 30 1014 1156 3864 Fragments arranged by size: 3864 1156 1014 30 ApoI r'AATT_y Cuts at: 1493 3373 3599 3751 5860 1493 Size: 1880 226 152 2109 1697 Fragments arranged by size: 2109 1880 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4618 160 Size: 872 3586 1606 Fragments arranged by size: 3586 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6064 AvaI C'yCGr_G Cuts at: 1870 1943 3389 1870 Size: 73 1446 4545 Fragments arranged by size: 4545 1446 73 BamHI G'GATC_C Cuts at: 2013 3394 3761 2013 Size: 1381 367 4316 Fragments arranged by size: 4316 1381 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2606 3385 4840 618 Size: 325 217 627 819 779 1455 1842 Fragments arranged by size: 1842 1455 819 779 627 325 217 BanII G_rGCy'C Cuts at: 1391 3276 3383 1391 Size: 1885 107 4072 Fragments arranged by size: 4072 1885 107 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2444 2987 928 Size: 971 545 543 4005 Fragments arranged by size: 4005 971 545 543 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4255 4289 1903 Size: 34 50 34 2234 34 3678 Fragments arranged by size: 3678 2234 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2836 3956 5166 2836 Size: 1120 1210 3734 Fragments arranged by size: 3734 1210 1120 BclI T'GATC_A Cuts at: 1548 1548 Size: 6064 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2604 2726 4675 Size: 107 122 71 631 605 932 122 1949 Cuts at: 4675 136 Size: 1525 Fragments arranged by size: 1949 1525 932 631 605 122 122 107 71 BglII A'GATC_T Cuts at: 6059 6059 Size: 6064 BlpI GC'TnA_GC Cuts at: 3315 3481 3315 Size: 166 5898 Bme1580I G_kGCm'C Cuts at: 1790 2071 2609 3081 3111 4125 5281 1790 Size: 281 538 472 30 1014 1156 2573 Fragments arranged by size: 2573 1156 1014 538 472 281 30 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6064 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3239 3795 4753 Size: 416 53 83 399 24 1822 556 958 Cuts at: 4753 442 Size: 1753 Fragments arranged by size: 1822 1753 958 556 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6064 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3066 3066 Size: 6064 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 2274 3038 4724 1239 Size: 917 118 764 1686 2579 Fragments arranged by size: 2579 1686 917 764 118 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4189 5054 5352 4189 Size: 865 298 4901 Fragments arranged by size: 4901 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4727 882 Size: 617 3228 2219 Fragments arranged by size: 3228 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3855 493 Size: 711 2651 2702 Fragments arranged by size: 2702 2651 711 BsaBI GATnn'nnATC Cuts at: 6058 6058 Size: 6064 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3871 4253 4888 275 Size: 53 83 186 936 2338 382 635 1451 Fragments arranged by size: 2338 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4497 4895 5169 5316 5727 4497 Size: 398 274 147 411 4834 Fragments arranged by size: 4834 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5839 Fragments arranged by size: 5839 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2393 1186 Size: 1207 4857 BseYI C'CCAG_C Cuts at: 2779 3358 5267 5763 2779 Size: 579 1909 496 3080 Fragments arranged by size: 3080 1909 579 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2483 2702 2984 1163 Size: 573 747 219 282 4243 Fragments arranged by size: 4243 747 573 282 219 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3514 4275 5303 1540 Size: 275 186 1513 761 1028 2301 Fragments arranged by size: 2301 1513 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3081 3111 4125 4210 5281 3081 Size: 30 1014 85 1071 3864 Fragments arranged by size: 3864 1071 1014 85 30 BsmI GAATG_Cn' Cuts at: 3340 3575 3668 3340 Size: 235 93 5736 Fragments arranged by size: 5736 235 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2071 2609 3081 3111 3276 3383 4125 Size: 399 281 538 472 30 165 107 742 Cuts at: 4125 4210 5281 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 538 472 399 281 165 107 85 30 BspEI T'CCGG_A Cuts at: 5727 5727 Size: 6064 BspHI T'CATG_A Cuts at: 2094 3953 5683 2094 Size: 1859 1730 2475 Fragments arranged by size: 2475 1859 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2454 3419 844 Size: 1610 965 3489 Fragments arranged by size: 3489 1610 965 BsrBI CCG'CTC Cuts at: 1394 1823 3951 1394 Size: 429 2128 3507 Fragments arranged by size: 3507 2128 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4559 4733 65 Size: 1183 3311 174 1396 Fragments arranged by size: 3311 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2361 4708 4895 1700 Size: 110 551 2347 187 2869 Fragments arranged by size: 2869 2347 551 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6064 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6064 BssSI C'ACGA_G Cuts at: 3110 4124 5136 3110 Size: 1014 1012 4038 Fragments arranged by size: 4038 1014 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2773 2804 794 Size: 1979 31 4054 Fragments arranged by size: 4054 1979 31 BstBI TT'CG_AA Cuts at: 4868 4868 Size: 6064 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6064 BstXI CCAn_nnnn'nTGG Cuts at: 2593 2884 3139 2593 Size: 291 255 5518 Fragments arranged by size: 5518 291 255 BstYI r'GATC_y Cuts at: 1972 2013 3394 3761 4146 4163 5615 5701 5713 Size: 41 1381 367 385 17 1452 86 12 Cuts at: 5713 6059 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1381 385 367 346 86 41 17 12 Bsu36I CC'TnA_GG Cuts at: 2742 2742 Size: 6064 BtgI C'CryG_G Cuts at: 513 1065 2115 513 Size: 552 1050 4462 Fragments arranged by size: 4462 1050 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5325 Fragments arranged by size: 5325 683 56 BtsI GCAGTG_nn' Cuts at: 2069 3580 4374 4394 2069 Size: 1511 794 20 3739 Fragments arranged by size: 3739 1511 794 20 ClaI AT'CG_AT Cuts at: 1554 2535 3176 1554 Size: 981 641 4442 Fragments arranged by size: 4442 981 641 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6029 DraI TTT'AAA Cuts at: 3368 3368 Size: 6064 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5071 809 Size: 507 3755 1802 Fragments arranged by size: 3755 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3129 3294 3511 Size: 54 1425 50 275 18 1299 165 217 Cuts at: 3511 4400 6029 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1299 889 275 217 165 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3511 1537 Size: 275 1699 4090 Fragments arranged by size: 4090 1699 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3994 1353 Size: 2641 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3055 4651 5025 5171 387 Size: 1064 138 1466 1596 374 146 1280 Fragments arranged by size: 1596 1466 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2274 2653 2922 3038 3110 4127 Size: 393 524 118 379 269 116 72 1017 Cuts at: 4127 4724 5511 5779 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1017 787 597 524 393 379 269 268 118 116 72 EcoO109I rG'GnC_Cy Cuts at: 2728 2728 Size: 6064 EcoRI G'AATT_C Cuts at: 3373 3751 3373 Size: 378 5686 FspI TGC'GCA Cuts at: 4570 4570 Size: 6064 HaeII r_GCGC'y Cuts at: 1678 2115 5211 1678 Size: 437 3096 2531 Fragments arranged by size: 3096 2531 437 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2247 2279 2872 2904 4705 Size: 32 135 32 675 32 593 32 1801 Cuts at: 4705 4737 4779 4811 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1801 675 593 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2476 3408 3654 669 Size: 988 281 538 932 246 3079 Fragments arranged by size: 3079 988 932 538 281 246 HindIII A'AGCT_T Cuts at: 748 2708 748 Size: 1960 4104 HpaI GTT'AAC Cuts at: 3654 3654 Size: 6064 KpnI G_GTAC'C Cuts at: 3389 3389 Size: 6064 MfeI C'AATT_G Cuts at: 3663 3663 Size: 6064 MluI A'CGCG_T Cuts at: 5940 5940 Size: 6064 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2683 5178 5362 1305 Size: 1378 2495 184 2007 Fragments arranged by size: 2495 2007 1378 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3131 3296 10 Size: 54 1425 343 1299 165 2778 Fragments arranged by size: 2778 1425 1299 343 165 54 MslI CAynn'nnrTG Cuts at: 518 1166 2285 2591 4022 4381 4540 518 Size: 648 1119 306 1431 359 159 2042 Fragments arranged by size: 2042 1431 1119 648 359 306 159 MspA1I CmG'CkG Cuts at: 1606 2224 2770 3121 3480 4157 5305 5550 1606 Size: 618 546 351 359 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 618 546 359 351 245 NcoI C'CATG_G Cuts at: 513 1065 2115 513 Size: 552 1050 4462 Fragments arranged by size: 4462 1050 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6064 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6064 NotI GC'GGCC_GC Cuts at: 3511 3511 Size: 6064 NspI r_CATG'y Cuts at: 1132 1229 2461 3422 4967 1132 Size: 97 1232 961 1545 2229 Fragments arranged by size: 2229 1545 1232 961 97 PasI CC'CwG_GG Cuts at: 1348 2063 2513 1348 Size: 715 450 4899 Fragments arranged by size: 4899 715 450 PciI A'CATG_T Cuts at: 4963 4963 Size: 6064 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6064 PfoI T'CCnGG_A Cuts at: 2133 2484 2838 3161 3282 2133 Size: 351 354 323 121 4915 Fragments arranged by size: 4915 354 351 323 121 PmeI GTTT'AAAC Cuts at: 3368 3368 Size: 6064 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4133 4165 5646 5678 4133 Size: 32 1481 32 4519 Fragments arranged by size: 4519 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2670 1613 Size: 1057 5007 PsiI TTA'TAA Cuts at: 3634 3634 Size: 6064 PstI C_TGCA'G Cuts at: 830 2036 2800 2817 3126 3416 830 Size: 1206 764 17 309 290 3478 Fragments arranged by size: 3478 1206 764 309 290 17 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6064 PvuII CAG'CTG Cuts at: 2224 2770 3121 2224 Size: 546 351 5167 Fragments arranged by size: 5167 546 351 SalI G'TCGA_C Cuts at: 1936 3406 1936 Size: 1470 4594 SbfI CC_TGCA'GG Cuts at: 3416 3416 Size: 6064 ScaI AGT'ACT Cuts at: 3101 4312 6024 3101 Size: 1211 1712 3141 Fragments arranged by size: 3141 1712 1211 SexAI A'CCwGG_T Cuts at: 2100 2100 Size: 6064 SfcI C'TryA_G Cuts at: 826 1045 2032 2796 2813 2910 3122 3412 4547 Size: 219 987 764 17 97 212 290 1135 Cuts at: 4547 5228 5419 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 987 764 681 290 219 212 191 97 17 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6064 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6064 SmaI CCC'GGG Cuts at: 1872 3391 1872 Size: 1519 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4168 5069 5331 820 Size: 197 926 2225 901 262 1553 Fragments arranged by size: 2225 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3855 493 Size: 3362 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6064 SphI G_CATG'C Cuts at: 1132 3422 1132 Size: 2290 3774 SspI AAT'ATT Cuts at: 5 52 3988 5 Size: 47 3936 2081 Fragments arranged by size: 3936 2081 47 StuI AGG'CCT Cuts at: 1517 2388 1517 Size: 871 5193 StyI C'CwwG_G Cuts at: 513 1065 2115 2967 3503 513 Size: 552 1050 852 536 3074 Fragments arranged by size: 3074 1050 852 552 536 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4091 4108 4261 4446 4891 325 Size: 1255 2511 17 153 185 445 1498 Fragments arranged by size: 2511 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3099 4310 6022 Size: 274 80 33 51 1443 1122 1211 1712 Cuts at: 6022 96 Size: 138 Fragments arranged by size: 1712 1443 1211 1122 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6024 6056 6024 Size: 32 6032 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6064 XbaI T'CTAG_A Cuts at: 3400 3400 Size: 6064 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2881 2881 Size: 6064 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6064 XmaI C'CCGG_G Cuts at: 1870 3389 1870 Size: 1519 4545 XmnI GAAnn'nnTTC Cuts at: 4193 4193 Size: 6064 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3872 276 Size: 53 83 186 936 2338 2468 Fragments arranged by size: 2468 2338 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI