Restriction map for
pFN21AB7225
(Circular) (Six-base) MAPSORT of: pFN21AB7225.seq Check: 8120 from: 1 to: 5491
pFN21AB7225 5491 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3301 278
Size: 53 83 186 936 1765 2468
Fragments arranged by size:
2468 1765 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2834 1937
Size: 897 4594
Acc65I G'GTAC_C
Cuts at: 2812 2812
Size: 5491
AclI AA'CG_TT
Cuts at: 1563 3618 3991 1563
Size: 2055 373 3063
Fragments arranged by size:
3063 2055 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2039 2196 2571 3554 4938 5206 1632
Size: 407 157 375 983 1384 268 1917
Fragments arranged by size:
1917 1384 983 407 375 268 157
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5294
AflIII A'CryG_T
Cuts at: 1203 4390 5367 1203
Size: 3187 977 1327
Fragments arranged by size:
3187 1327 977
AgeI A'CCGG_T
Cuts at: 4322 4322
Size: 5491
AhdI GACnn_n'nnGTC
Cuts at: 4220 4220
Size: 5491
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5491
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5491
AlwNI CAG_nnn'CTG
Cuts at: 3234 4806 3234
Size: 1572 3919
ApaI G_GGCC'C
Cuts at: 2479 2479
Size: 5491
ApaLI G'TGCA_C
Cuts at: 3548 4704 3548
Size: 1156 4335
ApoI r'AATT_y
Cuts at: 1493 2747 2800 3026 3178 5287 1493
Size: 1254 53 226 152 2109 1697
Fragments arranged by size:
2109 1697 1254 226 152 53
AseI AT'TA_AT
Cuts at: 160 1032 2118 4045 160
Size: 872 1086 1927 1606
Fragments arranged by size:
1927 1606 1086 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5491
AvaI C'yCGr_G
Cuts at: 1870 1943 2448 2816 1870
Size: 73 505 368 4545
Fragments arranged by size:
4545 505 368 73
BamHI G'GATC_C
Cuts at: 2821 3188 2821
Size: 367 5124
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2812 4267 618
Size: 325 217 627 1025 1455 1842
Fragments arranged by size:
1842 1455 1025 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2479 2810 1391
Size: 1088 331 4072
Fragments arranged by size:
4072 1088 331
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4520
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3682 3716 1903
Size: 34 1745 34 3678
Fragments arranged by size:
3678 1745 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3383 4593 3383
Size: 1210 4281
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5491
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4102 136
Size: 107 122 71 631 605 2430 1525
Fragments arranged by size:
2430 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5486 5486
Size: 5491
BlpI GC'TnA_GC
Cuts at: 2908 2908
Size: 5491
Bme1580I G_kGCm'C
Cuts at: 1790 2479 3552 4708 1790
Size: 689 1073 1156 2573
Fragments arranged by size:
2573 1156 1073 689
BmgBI CAC'GTC
Cuts at: 1318 2639 1318
Size: 1321 4170
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2118 3222 4180
Size: 416 53 83 399 24 701 1104 958
Cuts at: 4180 442
Size: 1753
Fragments arranged by size:
1753 1104 958 701 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5491
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4151 1239
Size: 2912 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3616 4481 4779 3616
Size: 865 298 4328
Fragments arranged by size:
4328 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4154 882
Size: 617 2655 2219
Fragments arranged by size:
2655 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3282 493
Size: 711 2078 2702
Fragments arranged by size:
2702 2078 711
BsaBI GATnn'nnATC
Cuts at: 5485 5485
Size: 5491
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3298 3680 4315 275
Size: 53 83 186 936 1765 382 635 1451
Fragments arranged by size:
1765 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2484 3924 4322 4596 4743 5154 2484
Size: 1440 398 274 147 411 2821
Fragments arranged by size:
2821 1440 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5266
Fragments arranged by size:
5266 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5491
BseYI C'CCAG_C
Cuts at: 4694 5190 4694
Size: 496 4995
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4918
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2941 3702 4730 1540
Size: 275 186 940 761 1028 2301
Fragments arranged by size:
2301 1028 940 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3552 3637 4708 3552
Size: 85 1071 4335
Fragments arranged by size:
4335 1071 85
BsmI GAATG_Cn'
Cuts at: 2606 3002 3095 2606
Size: 396 93 5002
Fragments arranged by size:
5002 396 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2479 2810 3552 3637 4708 1391
Size: 399 689 331 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 689 399 331 85
BspEI T'CCGG_A
Cuts at: 5154 5154
Size: 5491
BspHI T'CATG_A
Cuts at: 3380 5110 3380
Size: 1730 3761
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2846 844
Size: 2002 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3378 1394
Size: 429 1555 3507
Fragments arranged by size:
3507 1555 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2109 3986 4160 65
Size: 1183 861 1877 174 1396
Fragments arranged by size:
1877 1396 1183 861 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4135 4322 1700
Size: 110 2325 187 2869
Fragments arranged by size:
2869 2325 187 110
BsrGI T'GTAC_A
Cuts at: 96 2672 96
Size: 2576 2915
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5491
BssSI C'ACGA_G
Cuts at: 3551 4563 3551
Size: 1012 4479
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5491
BstBI TT'CG_AA
Cuts at: 4295 4295
Size: 5491
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5491
BstYI r'GATC_y
Cuts at: 1972 2690 2821 3188 3573 3590 5042 5128 5140
Size: 718 131 367 385 17 1452 86 12
Cuts at: 5140 5486 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 718 385 367 346 131 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4000
Fragments arranged by size:
4000 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4752
Fragments arranged by size:
4752 683 56
BtsI GCAGTG_nn'
Cuts at: 3007 3801 3821 3007
Size: 794 20 4677
Fragments arranged by size:
4677 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5491
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5456
DraI TTT'AAA
Cuts at: 2795 2795
Size: 5491
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4498 809
Size: 507 3182 1802
Fragments arranged by size:
3182 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2938 3827 5456
Size: 54 1425 50 275 18 1108 889 1629
Cuts at: 5456 8
Size: 43
Fragments arranged by size:
1629 1425 1108 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2938 1537
Size: 275 1126 4090
Fragments arranged by size:
4090 1126 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2036 3421 1353
Size: 683 1385 3423
Fragments arranged by size:
3423 1385 683
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4078 4452 4598 387
Size: 1064 138 2489 374 146 1280
Fragments arranged by size:
2489 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2196 2571 3554 4151 4938 5206
Size: 393 407 157 375 983 597 787 268
Cuts at: 5206 1239
Size: 1524
Fragments arranged by size:
1524 983 787 597 407 393 375 268
157
EcoRI G'AATT_C
Cuts at: 2800 3178 2800
Size: 378 5113
FspI TGC'GCA
Cuts at: 3997 3997
Size: 5491
HaeII r_GCGC'y
Cuts at: 1678 4638 1678
Size: 2960 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2448 2480 4132 4164 4206
Size: 32 135 32 876 32 1652 32 42
Cuts at: 4206 4238 1373
Size: 32 2626
Fragments arranged by size:
2626 1652 876 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2421 2835 3081 669
Size: 988 281 483 414 246 3079
Fragments arranged by size:
3079 988 483 414 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5491
HpaI GTT'AAC
Cuts at: 3081 3081
Size: 5491
KpnI G_GTAC'C
Cuts at: 2816 2816
Size: 5491
MfeI C'AATT_G
Cuts at: 3090 3090
Size: 5491
MluI A'CGCG_T
Cuts at: 5367 5367
Size: 5491
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4605 4789 1305
Size: 3300 184 2007
Fragments arranged by size:
3300 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3669
Fragments arranged by size:
3669 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3449 3808 3967 518
Size: 648 2283 359 159 2042
Fragments arranged by size:
2283 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2616 2907 3584 4732 4977 1606
Size: 1010 291 677 1148 245 2120
Fragments arranged by size:
2120 1148 1010 677 291 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4000
Fragments arranged by size:
4000 939 552
NdeI CA'TA_TG
Cuts at: 387 2296 387
Size: 1909 3582
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5491
NotI GC'GGCC_GC
Cuts at: 2938 2938
Size: 5491
NspI r_CATG'y
Cuts at: 1132 1229 2849 4394 1132
Size: 97 1620 1545 2229
Fragments arranged by size:
2229 1620 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5491
PciI A'CATG_T
Cuts at: 4390 4390
Size: 5491
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5491
PmeI GTTT'AAAC
Cuts at: 2795 2795
Size: 5491
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3560 3592 5073 5105 3560
Size: 32 1481 32 3946
Fragments arranged by size:
3946 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5491
PsiI TTA'TAA
Cuts at: 3061 3061
Size: 5491
PspOMI G'GGCC_C
Cuts at: 2475 2475
Size: 5491
PspXI vC'TCGA_Gb
Cuts at: 2448 2448
Size: 5491
PstI C_TGCA'G
Cuts at: 830 2843 830
Size: 2013 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5491
PvuII CAG'CTG
Cuts at: 2616 2616
Size: 5491
SalI G'TCGA_C
Cuts at: 1936 2833 1936
Size: 897 4594
SbfI CC_TGCA'GG
Cuts at: 2843 2843
Size: 5491
ScaI AGT'ACT
Cuts at: 2273 3739 5451 2273
Size: 1466 1712 2313
Fragments arranged by size:
2313 1712 1466
SfcI C'TryA_G
Cuts at: 826 1045 2839 3974 4655 4846 826
Size: 219 1794 1135 681 191 1471
Fragments arranged by size:
1794 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5491
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5491
SmaI CCC'GGG
Cuts at: 1872 2818 1872
Size: 946 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2448 3595 4496 4758 820
Size: 197 926 505 1147 901 262 1553
Fragments arranged by size:
1553 1147 926 901 505 262 197
SnaBI TAC'GTA
Cuts at: 493 3282 493
Size: 2789 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5491
SphI G_CATG'C
Cuts at: 1132 2849 1132
Size: 1717 3774
SspI AAT'ATT
Cuts at: 5 52 3415 5
Size: 47 3363 2081
Fragments arranged by size:
3363 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5491
StyI C'CwwG_G
Cuts at: 513 1065 2004 2703 2930 513
Size: 552 939 699 227 3074
Fragments arranged by size:
3074 939 699 552 227
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3518 3535 3688 3873 4318 325
Size: 1255 1938 17 153 185 445 1498
Fragments arranged by size:
1938 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2271 2672 2753
Size: 274 80 33 51 1443 294 401 81
Cuts at: 2753 3737 5449 96
Size: 984 1712 138
Fragments arranged by size:
1712 1443 984 401 294 274 138 81
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5451 5483 5451
Size: 32 5459
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5491
XbaI T'CTAG_A
Cuts at: 2827 2827
Size: 5491
XhoI C'TCGA_G
Cuts at: 1943 2448 1943
Size: 505 4986
XmaI C'CCGG_G
Cuts at: 1870 2816 1870
Size: 946 4545
XmnI GAAnn'nnTTC
Cuts at: 3620 3620
Size: 5491
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3299 276
Size: 53 83 186 936 1765 2468
Fragments arranged by size:
2468 1765 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI