Restriction map for
pFN21AB7224
(Circular) (Six-base) MAPSORT of: pFN21AB7224.seq Check: 5570 from: 1 to: 5773
pFN21AB7224 5773 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2042 2179 2042
Size: 137 5636
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3583 278
Size: 53 83 186 936 2047 2468
Fragments arranged by size:
2468 2047 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3116 1937
Size: 1179 4594
Acc65I G'GTAC_C
Cuts at: 3094 3094
Size: 5773
AclI AA'CG_TT
Cuts at: 1563 3900 4273 1563
Size: 2337 373 3063
Fragments arranged by size:
3063 2337 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2358 2898 3836 5220 5488 1632
Size: 726 540 938 1384 268 1917
Fragments arranged by size:
1917 1384 938 726 540 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5576
AflIII A'CryG_T
Cuts at: 1203 4672 5649 1203
Size: 3469 977 1327
Fragments arranged by size:
3469 1327 977
AgeI A'CCGG_T
Cuts at: 4604 4604
Size: 5773
AhdI GACnn_n'nnGTC
Cuts at: 4502 4502
Size: 5773
AleI CACnn'nnGTG
Cuts at: 1166 2456 1166
Size: 1290 4483
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5773
AlwNI CAG_nnn'CTG
Cuts at: 3516 5088 3516
Size: 1572 4201
ApaI G_GGCC'C
Cuts at: 2356 2584 2356
Size: 228 5545
ApaLI G'TGCA_C
Cuts at: 2313 3830 4986 2313
Size: 1517 1156 3100
Fragments arranged by size:
3100 1517 1156
ApoI r'AATT_y
Cuts at: 1493 3082 3308 3460 5569 1493
Size: 1589 226 152 2109 1697
Fragments arranged by size:
2109 1697 1589 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4327 160
Size: 872 3295 1606
Fragments arranged by size:
3295 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5773
AvaI C'yCGr_G
Cuts at: 1870 1943 2571 3098 1870
Size: 73 628 527 4545
Fragments arranged by size:
4545 628 527 73
BamHI G'GATC_C
Cuts at: 3103 3470 3103
Size: 367 5406
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3094 4549 618
Size: 325 217 627 1307 1455 1842
Fragments arranged by size:
1842 1455 1307 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2356 2442 2584 3092 1391
Size: 965 86 142 508 4072
Fragments arranged by size:
4072 965 508 142 86
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4802
BbvCI CC'TCA_GC
Cuts at: 2198 2522 2720 2870 2198
Size: 324 198 150 5101
Fragments arranged by size:
5101 324 198 150
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3964 3998 1903
Size: 34 2027 34 3678
Fragments arranged by size:
3678 2027 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3665 4875 3665
Size: 1210 4563
BclI T'GATC_A
Cuts at: 1548 2742 1548
Size: 1194 4579
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2516 4384 136
Size: 107 122 71 631 605 844 1868 1525
Fragments arranged by size:
1868 1525 844 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5768 5768
Size: 5773
BlpI GC'TnA_GC
Cuts at: 3190 3190
Size: 5773
Bme1580I G_kGCm'C
Cuts at: 1790 2317 2356 2584 2904 3834 4990 1790
Size: 527 39 228 320 930 1156 2573
Fragments arranged by size:
2573 1156 930 527 320 228 39
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5773
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3504 4462 442
Size: 416 53 83 399 24 2087 958 1753
Fragments arranged by size:
2087 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5773
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2650 2650
Size: 5773
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2620 4433 1239
Size: 1381 1813 2579
Fragments arranged by size:
2579 1813 1381
Bpu10I CC'TnA_GC
Cuts at: 2198 2522 2720 2870 2198
Size: 324 198 150 5101
Fragments arranged by size:
5101 324 198 150
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3898 4763 5061 3898
Size: 865 298 4610
Fragments arranged by size:
4610 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2015 4436 882
Size: 617 516 2421 2219
Fragments arranged by size:
2421 2219 617 516
BsaAI yAC'GTr
Cuts at: 493 1204 3564 493
Size: 711 2360 2702
Fragments arranged by size:
2702 2360 711
BsaBI GATnn'nnATC
Cuts at: 5767 5767
Size: 5773
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2142 2235 3580 3962
Size: 53 83 186 936 609 93 1345 382
Cuts at: 3962 4597 275
Size: 635 1451
Fragments arranged by size:
1451 1345 936 635 609 382 186 93
83 53
BsaWI w'CCGG_w
Cuts at: 4206 4604 4878 5025 5436 4206
Size: 398 274 147 411 4543
Fragments arranged by size:
4543 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2089 2119 2404 2434 1178
Size: 30 165 30 686 30 285 30 4517
Fragments arranged by size:
4517 686 285 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2436 2780 2952 1186
Size: 1250 344 172 4007
Fragments arranged by size:
4007 1250 344 172
BseYI C'CCAG_C
Cuts at: 2056 2535 4976 5472 2056
Size: 479 2441 496 2357
Fragments arranged by size:
2441 2357 496 479
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2378 1163
Size: 573 642 4558
Fragments arranged by size:
4558 642 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3223 3984 5012 1540
Size: 275 186 1222 761 1028 2301
Fragments arranged by size:
2301 1222 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2317 2467 3834 3919 4990 2317
Size: 150 1367 85 1071 3100
Fragments arranged by size:
3100 1367 1071 150 85
BsmI GAATG_Cn'
Cuts at: 3284 3377 3284
Size: 93 5680
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2317 2356 2442 2467 2584 2904 3092
Size: 399 527 39 86 25 117 320 188
Cuts at: 3092 3834 3919 4990 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 527 399 320 188 117
86 85 39 25
BspEI T'CCGG_A
Cuts at: 5436 5436
Size: 5773
BspHI T'CATG_A
Cuts at: 3662 5392 3662
Size: 1730 4043
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2028 2042 2179 3128 844
Size: 1184 14 137 949 3489
Fragments arranged by size:
3489 1184 949 137 14
BsrBI CCG'CTC
Cuts at: 1394 1823 3660 1394
Size: 429 1837 3507
Fragments arranged by size:
3507 1837 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4268 4442 65
Size: 1183 3020 174 1396
Fragments arranged by size:
3020 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4417 4604 1700
Size: 110 2607 187 2869
Fragments arranged by size:
2869 2607 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5773
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5773
BssSI C'ACGA_G
Cuts at: 2732 3833 4845 2732
Size: 1101 1012 3660
Fragments arranged by size:
3660 1101 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2277 3040 794
Size: 1483 763 3527
Fragments arranged by size:
3527 1483 763
BstBI TT'CG_AA
Cuts at: 4577 4577
Size: 5773
BstEII G'GTnAC_C
Cuts at: 1184 2029 1184
Size: 845 4928
BstXI CCAn_nnnn'nTGG
Cuts at: 2458 2458
Size: 5773
BstYI r'GATC_y
Cuts at: 1972 3010 3103 3470 3855 3872 5324 5410 5422
Size: 1038 93 367 385 17 1452 86 12
Cuts at: 5422 5768 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1038 385 367 346 93 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2759 2759
Size: 5773
BtgI C'CryG_G
Cuts at: 513 1065 2004 2337 2676 513
Size: 552 939 333 339 3610
Fragments arranged by size:
3610 939 552 339 333
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5034
Fragments arranged by size:
5034 683 56
BtsI GCAGTG_nn'
Cuts at: 2503 3289 4083 4103 2503
Size: 786 794 20 4173
Fragments arranged by size:
4173 794 786 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5773
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5738
DraI TTT'AAA
Cuts at: 3077 3077
Size: 5773
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2260 4780 809
Size: 507 944 2520 1802
Fragments arranged by size:
2520 1802 944 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2167 3220 4109
Size: 54 1425 50 275 18 337 1053 889
Cuts at: 4109 5738 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1053 889 337 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3220 1537
Size: 275 1408 4090
Fragments arranged by size:
4090 1408 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2595 3703 1353
Size: 1242 1108 3423
Fragments arranged by size:
3423 1242 1108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2792 2945 4360 4734 4880 387
Size: 1064 138 1203 153 1415 374 146 1280
Fragments arranged by size:
1415 1280 1203 1064 374 153 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2358 2620 2898 3836 4433 5220 5488
Size: 393 726 262 278 938 597 787 268
Cuts at: 5488 1239
Size: 1524
Fragments arranged by size:
1524 938 787 726 597 393 278 268
262
EcoNI CCTnn'n_nnAGG
Cuts at: 2373 2762 2373
Size: 389 5384
EcoO109I rG'GnC_Cy
Cuts at: 2040 2352 2353 2472 2040
Size: 312 1 119 5341
Fragments arranged by size:
5341 312 119 1
EcoRI G'AATT_C
Cuts at: 3082 3460 3082
Size: 378 5395
FspI TGC'GCA
Cuts at: 2281 4279 2281
Size: 1998 3775
HaeII r_GCGC'y
Cuts at: 1678 2522 2741 4920 1678
Size: 844 219 2179 2531
Fragments arranged by size:
2531 2179 844 219
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2186 2218 2404 2436 2814
Size: 32 135 32 614 32 186 32 378
Cuts at: 2814 2846 4414 4446 4488 4520 1373
Size: 32 1568 32 42 32 2626
Fragments arranged by size:
2626 1568 614 378 186 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3117 3363 669
Size: 988 281 1179 246 3079
Fragments arranged by size:
3079 1179 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5773
HpaI GTT'AAC
Cuts at: 3363 3363
Size: 5773
KpnI G_GTAC'C
Cuts at: 3098 3098
Size: 5773
MfeI C'AATT_G
Cuts at: 3372 3372
Size: 5773
MluI A'CGCG_T
Cuts at: 5649 5649
Size: 5773
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3027 4887 5071 1305
Size: 1722 1860 184 2007
Fragments arranged by size:
2007 1860 1722 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2169 10
Size: 54 1425 343 337 3614
Fragments arranged by size:
3614 1425 343 337 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2402 2456 3731 4090 4249 518
Size: 648 1236 54 1275 359 159 2042
Fragments arranged by size:
2042 1275 1236 648 359 159 54
MspA1I CmG'CkG
Cuts at: 1606 2658 2695 2724 3189 3866 5014 5259 1606
Size: 1052 37 29 465 677 1148 245 2120
Fragments arranged by size:
2120 1148 1052 677 465 245 37 29
NcoI C'CATG_G
Cuts at: 513 1065 2004 2337 2676 513
Size: 552 939 333 339 3610
Fragments arranged by size:
3610 939 552 339 333
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5773
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5773
NotI GC'GGCC_GC
Cuts at: 3220 3220
Size: 5773
NspI r_CATG'y
Cuts at: 1132 1229 3131 4676 1132
Size: 97 1902 1545 2229
Fragments arranged by size:
2229 1902 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2387 1348
Size: 1039 4734
PciI A'CATG_T
Cuts at: 4672 4672
Size: 5773
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2677 1108
Size: 1569 4204
PfoI T'CCnGG_A
Cuts at: 2229 2229
Size: 5773
PmeI GTTT'AAAC
Cuts at: 3077 3077
Size: 5773
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3842 3874 5355 5387 3842
Size: 32 1481 32 4228
Fragments arranged by size:
4228 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2040 2472 2040
Size: 432 5341
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5773
PsiI TTA'TAA
Cuts at: 3343 3343
Size: 5773
PspOMI G'GGCC_C
Cuts at: 2352 2580 2352
Size: 228 5545
PstI C_TGCA'G
Cuts at: 830 2039 3125 830
Size: 1209 1086 3478
Fragments arranged by size:
3478 1209 1086
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5773
PvuII CAG'CTG
Cuts at: 2724 2724
Size: 5773
SalI G'TCGA_C
Cuts at: 1936 3115 1936
Size: 1179 4594
SbfI CC_TGCA'GG
Cuts at: 2039 3125 2039
Size: 1086 4687
ScaI AGT'ACT
Cuts at: 4021 5733 4021
Size: 1712 4061
SfcI C'TryA_G
Cuts at: 826 1045 2035 2477 3121 4256 4937 5128 826
Size: 219 990 442 644 1135 681 191 1471
Fragments arranged by size:
1471 1135 990 681 644 442 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5773
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5773
SmaI CCC'GGG
Cuts at: 1872 2573 3100 1872
Size: 701 527 4545
Fragments arranged by size:
4545 701 527
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3877 4778 5040 820
Size: 197 926 1934 901 262 1553
Fragments arranged by size:
1934 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3564 493
Size: 3071 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5773
SphI G_CATG'C
Cuts at: 1132 3131 1132
Size: 1999 3774
SspI AAT'ATT
Cuts at: 5 52 3697 5
Size: 47 3645 2081
Fragments arranged by size:
3645 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5773
StyI C'CwwG_G
Cuts at: 513 1065 2004 2238 2337 2676 2983 3014 3212
Size: 552 939 234 99 339 307 31 198
Cuts at: 3212 513
Size: 3074
Fragments arranged by size:
3074 939 552 339 307 234 198 99
31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2450 3800 3817 3970 4155 4600 325
Size: 1255 870 1350 17 153 185 445 1498
Fragments arranged by size:
1498 1350 1255 870 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4019 5731 96
Size: 274 80 33 51 1443 2042 1712 138
Fragments arranged by size:
2042 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5733 5765 5733
Size: 32 5741
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5773
XbaI T'CTAG_A
Cuts at: 3109 3109
Size: 5773
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2335 2011
Size: 324 5449
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5773
XmaI C'CCGG_G
Cuts at: 1870 2571 3098 1870
Size: 701 527 4545
Fragments arranged by size:
4545 701 527
XmnI GAAnn'nnTTC
Cuts at: 3902 3902
Size: 5773
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3581 276
Size: 53 83 186 936 2047 2468
Fragments arranged by size:
2468 2047 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI