Restriction map for
pFN21AB7216
(Circular) (Six-base) MAPSORT of: pFN21AB7216.seq Check: 7716 from: 1 to: 5152
pFN21AB7216 5152 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2962 278
Size: 53 83 186 936 1426 2468
Fragments arranged by size:
2468 1426 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2495 1937
Size: 558 4594
Acc65I G'GTAC_C
Cuts at: 2473 2473
Size: 5152
AclI AA'CG_TT
Cuts at: 1563 3279 3652 1563
Size: 1716 373 3063
Fragments arranged by size:
3063 1716 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3215 4599 4867 1632
Size: 1583 1384 268 1917
Fragments arranged by size:
1917 1583 1384 268
AfeI AGC'GCT
Cuts at: 2016 2016
Size: 5152
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4955
AflIII A'CryG_T
Cuts at: 1203 4051 5028 1203
Size: 2848 977 1327
Fragments arranged by size:
2848 1327 977
AgeI A'CCGG_T
Cuts at: 3983 3983
Size: 5152
AhdI GACnn_n'nnGTC
Cuts at: 2078 3881 2078
Size: 1803 3349
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5152
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2081 1184
Size: 897 4255
AlwNI CAG_nnn'CTG
Cuts at: 2895 4467 2895
Size: 1572 3580
ApaI G_GGCC'C
Cuts at: 2211 2211
Size: 5152
ApaLI G'TGCA_C
Cuts at: 3209 4365 3209
Size: 1156 3996
ApoI r'AATT_y
Cuts at: 1493 2461 2687 2839 4948 1493
Size: 968 226 152 2109 1697
Fragments arranged by size:
2109 1697 968 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3706 160
Size: 872 2674 1606
Fragments arranged by size:
2674 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5152
AvaI C'yCGr_G
Cuts at: 1870 1943 2183 2477 1870
Size: 73 240 294 4545
Fragments arranged by size:
4545 294 240 73
AvrII C'CTAG_G
Cuts at: 2116 2116
Size: 5152
BamHI G'GATC_C
Cuts at: 2482 2849 2482
Size: 367 4785
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2120 2170 2473 3928 618
Size: 325 217 627 333 50 303 1455 1842
Fragments arranged by size:
1842 1455 627 333 325 303 217 50
BanII G_rGCy'C
Cuts at: 1391 2211 2268 2399 2471 1391
Size: 820 57 131 72 4072
Fragments arranged by size:
4072 820 131 72 57
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4181
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3343 3377 1903
Size: 34 1406 34 3678
Fragments arranged by size:
3678 1406 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3044 4254 3044
Size: 1210 3942
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5152
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3763 136
Size: 107 122 71 631 605 2091 1525
Fragments arranged by size:
2091 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5147 5147
Size: 5152
BlpI GC'TnA_GC
Cuts at: 2569 2569
Size: 5152
Bme1580I G_kGCm'C
Cuts at: 1790 2211 2294 3213 4369 1790
Size: 421 83 919 1156 2573
Fragments arranged by size:
2573 1156 919 421 83
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5152
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2883 3841 442
Size: 416 53 83 399 24 1466 958 1753
Fragments arranged by size:
1753 1466 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5152
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2075 2270 3812 1239
Size: 836 195 1542 2579
Fragments arranged by size:
2579 1542 836 195
Bpu10I CC'TnA_GC
Cuts at: 2267 2267
Size: 5152
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3277 4142 4440 3277
Size: 865 298 3989
Fragments arranged by size:
3989 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2169 3815 882
Size: 617 670 1646 2219
Fragments arranged by size:
2219 1646 670 617
BsaAI yAC'GTr
Cuts at: 493 1204 2943 493
Size: 711 1739 2702
Fragments arranged by size:
2702 1739 711
BsaBI GATnn'nnATC
Cuts at: 5146 5146
Size: 5152
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2959 3341 3976 275
Size: 53 83 186 936 1426 382 635 1451
Fragments arranged by size:
1451 1426 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2363 3585 3983 4257 4404 4815 2363
Size: 1222 398 274 147 411 2700
Fragments arranged by size:
2700 1222 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4927
Fragments arranged by size:
4927 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5152
BseYI C'CCAG_C
Cuts at: 4355 4851 4355
Size: 496 4656
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4579
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2602 3363 4391 1540
Size: 275 186 601 761 1028 2301
Fragments arranged by size:
2301 1028 761 601 275 186
BsiHKAI G_wGCw'C
Cuts at: 2268 3213 3298 4369 2268
Size: 945 85 1071 3051
Fragments arranged by size:
3051 1071 945 85
BsmI GAATG_Cn'
Cuts at: 2663 2756 2663
Size: 93 5059
BsmBI CGTCTCn'nnnn_
Cuts at: 2086 2086
Size: 5152
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2211 2268 2294 2399 2471 3213 3298
Size: 399 421 57 26 105 72 742 85
Cuts at: 3298 4369 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 421 399 105 85 72
57 26
BspEI T'CCGG_A
Cuts at: 4815 4815
Size: 5152
BspHI T'CATG_A
Cuts at: 3041 4771 3041
Size: 1730 3422
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2507 844
Size: 1663 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3039 1394
Size: 429 1216 3507
Fragments arranged by size:
3507 1216 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3647 3821 65
Size: 1183 2399 174 1396
Fragments arranged by size:
2399 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3796 3983 1700
Size: 110 1986 187 2869
Fragments arranged by size:
2869 1986 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5152
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5152
BssSI C'ACGA_G
Cuts at: 2059 3212 4224 2059
Size: 1153 1012 2987
Fragments arranged by size:
2987 1153 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5152
BstBI TT'CG_AA
Cuts at: 3956 3956
Size: 5152
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5152
BstYI r'GATC_y
Cuts at: 1972 2482 2849 3234 3251 4703 4789 4801 5147
Size: 510 367 385 17 1452 86 12 346
Cuts at: 5147 1972
Size: 1977
Fragments arranged by size:
1977 1452 510 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4600
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4413
Fragments arranged by size:
4413 683 56
BtsI GCAGTG_nn'
Cuts at: 2047 2668 3462 3482 2047
Size: 621 794 20 3717
Fragments arranged by size:
3717 794 621 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5152
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5117
DraI TTT'AAA
Cuts at: 2456 2456
Size: 5152
DraIII CAC_nnn'GTG
Cuts at: 2300 2300
Size: 5152
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4159 809
Size: 507 2843 1802
Fragments arranged by size:
2843 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2599 3488 5117
Size: 54 1425 50 275 18 769 889 1629
Cuts at: 5117 8
Size: 43
Fragments arranged by size:
1629 1425 889 769 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2599 1537
Size: 275 787 4090
Fragments arranged by size:
4090 787 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3082 1353
Size: 1729 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3739 4113 4259 387
Size: 1064 138 2150 374 146 1280
Fragments arranged by size:
2150 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2266 2266
Size: 5152
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2270 3215 3812 4599 4867 1239
Size: 393 443 195 945 597 787 268 1524
Fragments arranged by size:
1524 945 787 597 443 393 268 195
EcoNI CCTnn'n_nnAGG
Cuts at: 2129 2129
Size: 5152
EcoO109I rG'GnC_Cy
Cuts at: 2096 2208 2096
Size: 112 5040
EcoRI G'AATT_C
Cuts at: 2461 2839 2461
Size: 378 4774
FspI TGC'GCA
Cuts at: 3658 3658
Size: 5152
HaeII r_GCGC'y
Cuts at: 1678 2018 4299 1678
Size: 340 2281 2531
Fragments arranged by size:
2531 2281 340
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2064 2096 2268 2300 3793
Size: 32 135 32 492 32 172 32 1493
Cuts at: 3793 3825 3867 3899 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1493 492 172 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2496 2742 669
Size: 988 281 558 246 3079
Fragments arranged by size:
3079 988 558 281 246
HindIII A'AGCT_T
Cuts at: 748 2144 748
Size: 1396 3756
HpaI GTT'AAC
Cuts at: 2742 2742
Size: 5152
KpnI G_GTAC'C
Cuts at: 2477 2477
Size: 5152
MfeI C'AATT_G
Cuts at: 2751 2751
Size: 5152
MluI A'CGCG_T
Cuts at: 5028 5028
Size: 5152
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2120 4266 4450 1305
Size: 815 2146 184 2007
Fragments arranged by size:
2146 2007 815 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3330
Fragments arranged by size:
3330 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3110 3469 3628 518
Size: 648 1944 359 159 2042
Fragments arranged by size:
2042 1944 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2037 2374 2568 3245 4393 4638 1606
Size: 431 337 194 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 431 337 245 194
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4600
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5152
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5152
NotI GC'GGCC_GC
Cuts at: 2599 2599
Size: 5152
NspI r_CATG'y
Cuts at: 1132 1229 2510 4055 1132
Size: 97 1281 1545 2229
Fragments arranged by size:
2229 1545 1281 97
PasI CC'CwG_GG
Cuts at: 1348 2125 1348
Size: 777 4375
PciI A'CATG_T
Cuts at: 4051 4051
Size: 5152
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5152
PmeI GTTT'AAAC
Cuts at: 2456 2456
Size: 5152
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3221 3253 4734 4766 3221
Size: 32 1481 32 3607
Fragments arranged by size:
3607 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2096 2096
Size: 5152
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5152
PsiI TTA'TAA
Cuts at: 2722 2722
Size: 5152
PspOMI G'GGCC_C
Cuts at: 2207 2207
Size: 5152
PstI C_TGCA'G
Cuts at: 830 2042 2504 830
Size: 1212 462 3478
Fragments arranged by size:
3478 1212 462
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5152
PvuII CAG'CTG
Cuts at: 2374 2374
Size: 5152
SacI G_AGCT'C
Cuts at: 2268 2268
Size: 5152
SalI G'TCGA_C
Cuts at: 1936 2494 1936
Size: 558 4594
SanDI GG'GwC_CC
Cuts at: 2096 2096
Size: 5152
SbfI CC_TGCA'GG
Cuts at: 2504 2504
Size: 5152
ScaI AGT'ACT
Cuts at: 3400 5112 3400
Size: 1712 3440
SfcI C'TryA_G
Cuts at: 826 1045 2038 2500 3635 4316 4507 826
Size: 219 993 462 1135 681 191 1471
Fragments arranged by size:
1471 1135 993 681 462 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5152
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5152
SmaI CCC'GGG
Cuts at: 1872 2479 1872
Size: 607 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3256 4157 4419 820
Size: 197 926 1313 901 262 1553
Fragments arranged by size:
1553 1313 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2943 493
Size: 2450 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5152
SphI G_CATG'C
Cuts at: 1132 2510 1132
Size: 1378 3774
SspI AAT'ATT
Cuts at: 5 52 3076 5
Size: 47 3024 2081
Fragments arranged by size:
3024 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5152
StyI C'CwwG_G
Cuts at: 513 1065 2116 2591 513
Size: 552 1051 475 3074
Fragments arranged by size:
3074 1051 552 475
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2213 2235 3179 3196 3349 3534 3979
Size: 1255 633 22 944 17 153 185 445
Cuts at: 3979 325
Size: 1498
Fragments arranged by size:
1498 1255 944 633 445 185 153 22
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3398 5110 96
Size: 274 80 33 51 1443 1421 1712 138
Fragments arranged by size:
1712 1443 1421 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5112 5144 5112
Size: 32 5120
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5152
XbaI T'CTAG_A
Cuts at: 2488 2488
Size: 5152
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5152
XmaI C'CCGG_G
Cuts at: 1870 2477 1870
Size: 607 4545
XmnI GAAnn'nnTTC
Cuts at: 3281 3281
Size: 5152
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2960 276
Size: 53 83 186 936 1426 2468
Fragments arranged by size:
2468 1426 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BbeI BbvCI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI
SfoI SrfI SwaI XcmI