Restriction map for
pFN21AB7214
(Circular) (Six-base) MAPSORT of: pFN21AB7214.seq Check: 2138 from: 1 to: 5584
pFN21AB7214 5584 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2275 2275
Size: 5584
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3394 278
Size: 53 83 186 936 1858 2468
Fragments arranged by size:
2468 1858 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2927 1937
Size: 990 4594
Acc65I G'GTAC_C
Cuts at: 2905 2905
Size: 5584
AclI AA'CG_TT
Cuts at: 1563 3711 4084 1563
Size: 2148 373 3063
Fragments arranged by size:
3063 2148 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2039 2187 2645 3647 5031 5299 1632
Size: 407 148 458 1002 1384 268 1917
Fragments arranged by size:
1917 1384 1002 458 407 268 148
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5387
AflIII A'CryG_T
Cuts at: 1203 4483 5460 1203
Size: 3280 977 1327
Fragments arranged by size:
3280 1327 977
AgeI A'CCGG_T
Cuts at: 4415 4415
Size: 5584
AhdI GACnn_n'nnGTC
Cuts at: 4313 4313
Size: 5584
AleI CACnn'nnGTG
Cuts at: 1166 2218 2345 2432 1166
Size: 1052 127 87 4318
Fragments arranged by size:
4318 1052 127 87
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2380 1184
Size: 1196 4388
AlwNI CAG_nnn'CTG
Cuts at: 2165 2604 3327 4899 2165
Size: 439 723 1572 2850
Fragments arranged by size:
2850 1572 723 439
ApaI G_GGCC'C
Cuts at: 2177 2177
Size: 5584
ApaLI G'TGCA_C
Cuts at: 3641 4797 3641
Size: 1156 4428
ApoI r'AATT_y
Cuts at: 1493 2533 2575 2655 2893 3119 3271 5380 1493
Size: 1040 42 80 238 226 152 2109 1697
Fragments arranged by size:
2109 1697 1040 238 226 152 80 42
AseI AT'TA_AT
Cuts at: 160 1032 4138 160
Size: 872 3106 1606
Fragments arranged by size:
3106 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5584
AvaI C'yCGr_G
Cuts at: 1870 1943 2025 2066 2909 1870
Size: 73 82 41 843 4545
Fragments arranged by size:
4545 843 82 73 41
BamHI G'GATC_C
Cuts at: 2914 3281 2914
Size: 367 5217
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2092 2324 2905 4360 618
Size: 325 217 627 305 232 581 1455 1842
Fragments arranged by size:
1842 1455 627 581 325 305 232 217
BanII G_rGCy'C
Cuts at: 1391 2153 2177 2903 1391
Size: 762 24 726 4072
Fragments arranged by size:
4072 762 726 24
BbeI G_GCGC'C
Cuts at: 2096 2096
Size: 5584
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4613
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3775 3809 1903
Size: 34 1838 34 3678
Fragments arranged by size:
3678 1838 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3476 4686 3476
Size: 1210 4374
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5584
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4195 136
Size: 107 122 71 631 605 2523 1525
Fragments arranged by size:
2523 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5579 5579
Size: 5584
BlpI GC'TnA_GC
Cuts at: 3001 3001
Size: 5584
Bme1580I G_kGCm'C
Cuts at: 1790 2177 3645 4801 1790
Size: 387 1468 1156 2573
Fragments arranged by size:
2573 1468 1156 387
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5584
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2086 3315 4273
Size: 416 53 83 399 24 669 1229 958
Cuts at: 4273 442
Size: 1753
Fragments arranged by size:
1753 1229 958 669 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5584
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2172 2172
Size: 5584
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2142 2498 4244 1239
Size: 903 356 1746 2579
Fragments arranged by size:
2579 1746 903 356
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3709 4574 4872 3709
Size: 865 298 4421
Fragments arranged by size:
4421 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2446 4247 882
Size: 617 947 1801 2219
Fragments arranged by size:
2219 1801 947 617
BsaAI yAC'GTr
Cuts at: 493 1204 2343 3375 493
Size: 711 1139 1032 2702
Fragments arranged by size:
2702 1139 1032 711
BsaBI GATnn'nnATC
Cuts at: 5578 5578
Size: 5584
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2093 3391 3773 4408
Size: 53 83 186 936 560 1298 382 635
Cuts at: 4408 275
Size: 1451
Fragments arranged by size:
1451 1298 936 635 560 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2612 4017 4415 4689 4836 5247 2612
Size: 1405 398 274 147 411 2949
Fragments arranged by size:
2949 1405 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5359
Fragments arranged by size:
5359 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2105 2138 2265 1186
Size: 919 33 127 4505
Fragments arranged by size:
4505 919 127 33
BseYI C'CCAG_C
Cuts at: 2604 2862 4787 5283 2604
Size: 258 1925 496 2905
Fragments arranged by size:
2905 1925 496 258
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5011
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3034 3795 4823 1540
Size: 275 186 1033 761 1028 2301
Fragments arranged by size:
2301 1033 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3645 3730 4801 3645
Size: 85 1071 4428
Fragments arranged by size:
4428 1071 85
BsmI GAATG_Cn'
Cuts at: 3095 3188 3095
Size: 93 5491
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2153 2177 2903 3645 3730 4801 1391
Size: 399 363 24 726 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 726 399 363 85 24
BspEI T'CCGG_A
Cuts at: 5247 5247
Size: 5584
BspHI T'CATG_A
Cuts at: 3473 5203 3473
Size: 1730 3854
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2275 2939 844
Size: 1431 664 3489
Fragments arranged by size:
3489 1431 664
BsrBI CCG'CTC
Cuts at: 1394 1823 3471 1394
Size: 429 1648 3507
Fragments arranged by size:
3507 1648 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4079 4253 65
Size: 1183 2831 174 1396
Fragments arranged by size:
2831 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4228 4415 1700
Size: 110 2418 187 2869
Fragments arranged by size:
2869 2418 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5584
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5584
BssSI C'ACGA_G
Cuts at: 3644 4656 3644
Size: 1012 4572
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5584
BstBI TT'CG_AA
Cuts at: 2494 4388 2494
Size: 1894 3690
BstEII G'GTnAC_C
Cuts at: 1184 2332 2608 1184
Size: 1148 276 4160
Fragments arranged by size:
4160 1148 276
BstYI r'GATC_y
Cuts at: 1972 2914 3281 3666 3683 5135 5221 5233 5579
Size: 942 367 385 17 1452 86 12 346
Cuts at: 5579 1972
Size: 1977
Fragments arranged by size:
1977 1452 942 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2568 2568
Size: 5584
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5032
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4845
Fragments arranged by size:
4845 683 56
BtsI GCAGTG_nn'
Cuts at: 2213 2362 3100 3894 3914 2213
Size: 149 738 794 20 3883
Fragments arranged by size:
3883 794 738 149 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5584
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2418 2453 529
Size: 35 1854 35 3660
Fragments arranged by size:
3660 1854 35 35
DraI TTT'AAA
Cuts at: 2888 2888
Size: 5584
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2840 4591 809
Size: 507 1524 1751 1802
Fragments arranged by size:
1802 1751 1524 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2224 3031 3920
Size: 54 1425 50 275 18 394 807 889
Cuts at: 3920 5549 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 807 394 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3031 1537
Size: 275 1219 4090
Fragments arranged by size:
4090 1219 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3514 1353
Size: 2161 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4171 4545 4691 387
Size: 1064 138 2582 374 146 1280
Fragments arranged by size:
2582 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2142 2187 2498 2645 3647 4244
Size: 393 407 103 45 311 147 1002 597
Cuts at: 4244 5031 5299 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1002 787 597 407 393 311 268
147 103 45
EcoNI CCTnn'n_nnAGG
Cuts at: 2120 2120
Size: 5584
EcoO109I rG'GnC_Cy
Cuts at: 2029 2162 2174 2029
Size: 133 12 5439
Fragments arranged by size:
5439 133 12
EcoRI G'AATT_C
Cuts at: 2893 3271 2893
Size: 378 5206
FspI TGC'GCA
Cuts at: 4090 4090
Size: 5584
HaeII r_GCGC'y
Cuts at: 1678 2096 4731 1678
Size: 418 2635 2531
Fragments arranged by size:
2635 2531 418
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2389 2421 2445 2477 4225
Size: 32 135 32 817 32 24 32 1748
Cuts at: 4225 4257 4299 4331 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1748 817 135 42 32 32 32
32 32 32 24
HincII GTy'rAC
Cuts at: 669 1657 1938 2928 3174 669
Size: 988 281 990 246 3079
Fragments arranged by size:
3079 990 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5584
HpaI GTT'AAC
Cuts at: 3174 3174
Size: 5584
KasI G'GCGC_C
Cuts at: 2092 2092
Size: 5584
KpnI G_GTAC'C
Cuts at: 2909 2909
Size: 5584
MfeI C'AATT_G
Cuts at: 3183 3183
Size: 5584
MluI A'CGCG_T
Cuts at: 5460 5460
Size: 5584
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2545 4698 4882 1305
Size: 1240 2153 184 2007
Fragments arranged by size:
2153 2007 1240 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3762
Fragments arranged by size:
3762 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2218 2345 2388 2432 3542 3901 4060
Size: 648 1052 127 43 44 1110 359 159
Cuts at: 4060 518
Size: 2042
Fragments arranged by size:
2042 1110 1052 648 359 159 127 44
43
MspA1I CmG'CkG
Cuts at: 1606 3000 3677 4825 5070 1606
Size: 1394 677 1148 245 2120
Fragments arranged by size:
2120 1394 1148 677 245
NarI GG'CG_CC
Cuts at: 2093 2093
Size: 5584
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5032
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5584
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5584
NotI GC'GGCC_GC
Cuts at: 3031 3031
Size: 5584
NspI r_CATG'y
Cuts at: 1132 1229 2942 4487 1132
Size: 97 1713 1545 2229
Fragments arranged by size:
2229 1713 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5584
PciI A'CATG_T
Cuts at: 4483 4483
Size: 5584
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2604 1108
Size: 1496 4088
PfoI T'CCnGG_A
Cuts at: 2158 2158
Size: 5584
PmeI GTTT'AAAC
Cuts at: 2888 2888
Size: 5584
PmlI CAC'GTG
Cuts at: 2343 2343
Size: 5584
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3653 3685 5166 5198 3653
Size: 32 1481 32 4039
Fragments arranged by size:
4039 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2162 2162
Size: 5584
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5584
PsiI TTA'TAA
Cuts at: 3154 3154
Size: 5584
PspOMI G'GGCC_C
Cuts at: 2173 2173
Size: 5584
PstI C_TGCA'G
Cuts at: 830 2936 830
Size: 2106 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5584
SalI G'TCGA_C
Cuts at: 1936 2926 1936
Size: 990 4594
SbfI CC_TGCA'GG
Cuts at: 2936 2936
Size: 5584
ScaI AGT'ACT
Cuts at: 3832 5544 3832
Size: 1712 3872
SexAI A'CCwGG_T
Cuts at: 2597 2597
Size: 5584
SfcI C'TryA_G
Cuts at: 826 1045 2932 4067 4748 4939 826
Size: 219 1887 1135 681 191 1471
Fragments arranged by size:
1887 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2094 2094
Size: 5584
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5584
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5584
SmaI CCC'GGG
Cuts at: 1872 2027 2068 2911 1872
Size: 155 41 843 4545
Fragments arranged by size:
4545 843 155 41
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3688 4589 4851 820
Size: 197 926 1745 901 262 1553
Fragments arranged by size:
1745 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3375 493
Size: 2882 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5584
SphI G_CATG'C
Cuts at: 1132 2942 1132
Size: 1810 3774
SrfI GCCC'GGGC
Cuts at: 2068 2068
Size: 5584
SspI AAT'ATT
Cuts at: 5 52 3508 5
Size: 47 3456 2081
Fragments arranged by size:
3456 2081 47
StuI AGG'CCT
Cuts at: 1517 2720 1517
Size: 1203 4381
StyI C'CwwG_G
Cuts at: 513 1065 3023 513
Size: 552 1958 3074
Fragments arranged by size:
3074 1958 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2335 2596 3611 3628 3781 3966 4411
Size: 1255 755 261 1015 17 153 185 445
Cuts at: 4411 325
Size: 1498
Fragments arranged by size:
1498 1255 1015 755 445 261 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3830 5542 96
Size: 274 80 33 51 1443 1853 1712 138
Fragments arranged by size:
1853 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2258 2290 2354 2386 2746 2778 5544 5576 2258
Size: 32 64 32 360 32 2766 32 2266
Fragments arranged by size:
2766 2266 360 64 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5584
XbaI T'CTAG_A
Cuts at: 2920 2920
Size: 5584
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5584
XmaI C'CCGG_G
Cuts at: 1870 2025 2066 2909 1870
Size: 155 41 843 4545
Fragments arranged by size:
4545 843 155 41
XmnI GAAnn'nnTTC
Cuts at: 2658 2785 3713 2658
Size: 127 928 4529
Fragments arranged by size:
4529 928 127
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3392 276
Size: 53 83 186 936 1858 2468
Fragments arranged by size:
2468 1858 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SfiI
SwaI XcmI