Restriction map for
pFN21AB7205
(Circular) (Six-base) MAPSORT of: pFN21AB7205.seq Check: 7903 from: 1 to: 5983
pFN21AB7205 5983 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3042 3042
Size: 5983
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3793 278
Size: 53 83 186 936 2257 2468
Fragments arranged by size:
2468 2257 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3326 1937
Size: 1389 4594
Acc65I G'GTAC_C
Cuts at: 3304 3304
Size: 5983
AclI AA'CG_TT
Cuts at: 1563 4110 4483 1563
Size: 2547 373 3063
Fragments arranged by size:
3063 2547 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4046 5430 5698 1632
Size: 2414 1384 268 1917
Fragments arranged by size:
2414 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5786
AflIII A'CryG_T
Cuts at: 1203 4882 5859 1203
Size: 3679 977 1327
Fragments arranged by size:
3679 1327 977
AgeI A'CCGG_T
Cuts at: 4814 4814
Size: 5983
AhdI GACnn_n'nnGTC
Cuts at: 4712 4712
Size: 5983
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5983
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5983
AlwNI CAG_nnn'CTG
Cuts at: 2239 2830 3726 5298 2239
Size: 591 896 1572 2924
Fragments arranged by size:
2924 1572 896 591
ApaI G_GGCC'C
Cuts at: 2083 2140 2747 2899 3278 2083
Size: 57 607 152 379 4788
Fragments arranged by size:
4788 607 379 152 57
ApaLI G'TGCA_C
Cuts at: 2426 4040 5196 2426
Size: 1614 1156 3213
Fragments arranged by size:
3213 1614 1156
ApoI r'AATT_y
Cuts at: 1493 3292 3518 3670 5779 1493
Size: 1799 226 152 2109 1697
Fragments arranged by size:
2109 1799 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4537 160
Size: 872 3505 1606
Fragments arranged by size:
3505 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5983
AvaI C'yCGr_G
Cuts at: 1870 1943 2076 2378 2399 2751 2817 3056 3308
Size: 73 133 302 21 352 66 239 252
Cuts at: 3308 1870
Size: 4545
Fragments arranged by size:
4545 352 302 252 239 133 73 66
21
BamHI G'GATC_C
Cuts at: 2784 3313 3680 2784
Size: 529 367 5087
Fragments arranged by size:
5087 529 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2434 3060 3178 3255 3304
Size: 325 217 627 647 626 118 77 49
Cuts at: 3304 4759 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 647 627 626 325 217 118
77 49
BanII G_rGCy'C
Cuts at: 1391 2083 2140 2399 2737 2747 2899 3278 3302
Size: 692 57 259 338 10 152 379 24
Cuts at: 3302 1391
Size: 4072
Fragments arranged by size:
4072 692 379 338 259 152 57 24
10
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2654 928
Size: 971 755 4257
Fragments arranged by size:
4257 971 755
BbvCI CC'TCA_GC
Cuts at: 2445 2459 2445
Size: 14 5969
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2407 2441 4174 4208 1903
Size: 34 470 34 1733 34 3678
Fragments arranged by size:
3678 1733 470 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3875 5085 3875
Size: 1210 4773
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5983
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2144 2516 4594
Size: 107 122 71 631 605 472 372 2078
Cuts at: 4594 136
Size: 1525
Fragments arranged by size:
2078 1525 631 605 472 372 122 107
71
BglII A'GATC_T
Cuts at: 2712 5978 2712
Size: 3266 2717
BlpI GC'TnA_GC
Cuts at: 3400 3400
Size: 5983
Bme1580I G_kGCm'C
Cuts at: 1790 2083 2140 2353 2430 2439 2747 2899 3065
Size: 293 57 213 77 9 308 152 166
Cuts at: 3065 3183 3278 4044 5200 1790
Size: 118 95 766 1156 2573
Fragments arranged by size:
2573 1156 766 308 293 213 166 152
118 95 77 57 9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5983
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3714 4672 442
Size: 416 53 83 399 24 2297 958 1753
Fragments arranged by size:
2297 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5983
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2197 2197
Size: 5983
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2261 2363 2373 2431 2561 2591 2873 3156
Size: 1022 102 10 58 130 30 282 283
Cuts at: 3156 3265 4643 1239
Size: 109 1378 2579
Fragments arranged by size:
2579 1378 1022 283 282 130 109 102
58 30 10
Bpu10I CC'TnA_GC
Cuts at: 2445 2459 2445
Size: 14 5969
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2828 4108 4973 5271 2828
Size: 1280 865 298 3540
Fragments arranged by size:
3540 1280 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3136 3241 4646 882
Size: 617 1637 105 1405 2219
Fragments arranged by size:
2219 1637 1405 617 105
BsaAI yAC'GTr
Cuts at: 493 1204 2740 3774 493
Size: 711 1536 1034 2702
Fragments arranged by size:
2702 1536 1034 711
BsaBI GATnn'nnATC
Cuts at: 5977 5977
Size: 5983
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3790 4172 4807 275
Size: 53 83 186 936 2257 382 635 1451
Fragments arranged by size:
2257 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2287 2955 4416 4814 5088 5235 5646 2287
Size: 668 1461 398 274 147 411 2624
Fragments arranged by size:
2624 1461 668 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2294 2324 2724 2754 2979
Size: 30 165 30 891 30 400 30 225
Cuts at: 2979 3009 1178
Size: 30 4152
Fragments arranged by size:
4152 891 400 225 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2275 2318 1186
Size: 1089 43 4851
Fragments arranged by size:
4851 1089 43
BseYI C'CCAG_C
Cuts at: 2245 2769 3166 3229 5186 5682 2245
Size: 524 397 63 1957 496 2546
Fragments arranged by size:
2546 1957 524 496 397 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5410
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2134 3433 4194 5222 1540
Size: 275 186 133 1299 761 1028 2301
Fragments arranged by size:
2301 1299 1028 761 275 186 133
BsiHKAI G_wGCw'C
Cuts at: 2430 4044 4129 5200 2430
Size: 1614 85 1071 3213
Fragments arranged by size:
3213 1614 1071 85
BsmI GAATG_Cn'
Cuts at: 2800 3494 3587 2800
Size: 694 93 5196
Fragments arranged by size:
5196 694 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3007 3007
Size: 5983
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2083 2140 2353 2399 2430 2439 2737
Size: 399 293 57 213 46 31 9 298
Cuts at: 2737 2747 2899 3065 3183 3278 3302 4044 4129
Size: 10 152 166 118 95 24 742 85
Cuts at: 4129 5200 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 298 293 213 166
152 118 95 85 57 46 31 24
10 9
BspEI T'CCGG_A
Cuts at: 2287 2955 5646 2287
Size: 668 2691 2624
Fragments arranged by size:
2691 2624 668
BspHI T'CATG_A
Cuts at: 2866 3872 5602 2866
Size: 1006 1730 3247
Fragments arranged by size:
3247 1730 1006
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3042 3338 844
Size: 2198 296 3489
Fragments arranged by size:
3489 2198 296
BsrBI CCG'CTC
Cuts at: 1394 1823 3870 1394
Size: 429 2047 3507
Fragments arranged by size:
3507 2047 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3025 4478 4652 65
Size: 1183 1777 1453 174 1396
Fragments arranged by size:
1777 1453 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2009 2295 4627 4814 1700
Size: 110 199 286 2332 187 2869
Fragments arranged by size:
2869 2332 286 199 187 110
BsrGI T'GTAC_A
Cuts at: 96 2310 96
Size: 2214 3769
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5983
BssSI C'ACGA_G
Cuts at: 2196 2492 4043 5055 2196
Size: 296 1551 1012 3124
Fragments arranged by size:
3124 1551 1012 296
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2434 794
Size: 1640 4343
BstBI TT'CG_AA
Cuts at: 4787 4787
Size: 5983
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5983
BstXI CCAn_nnnn'nTGG
Cuts at: 2036 2036
Size: 5983
BstYI r'GATC_y
Cuts at: 1972 2712 2784 3313 3680 4065 4082 5534 5620
Size: 740 72 529 367 385 17 1452 86
Cuts at: 5620 5632 5978 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 740 529 385 367 346 86
72 17 12
Bsu36I CC'TnA_GG
Cuts at: 2258 2258
Size: 5983
BtgI C'CryG_G
Cuts at: 513 1065 2004 2125 2157 2217 2439 2656 3124
Size: 552 939 121 32 60 222 217 468
Cuts at: 3124 3139 3279 513
Size: 15 140 3217
Fragments arranged by size:
3217 939 552 468 222 217 140 121
60 32 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5244
Fragments arranged by size:
5244 683 56
BtsI GCAGTG_nn'
Cuts at: 2606 3499 4293 4313 2606
Size: 893 794 20 4276
Fragments arranged by size:
4276 893 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5983
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5948
DraI TTT'AAA
Cuts at: 3287 3287
Size: 5983
DraIII CAC_nnn'GTG
Cuts at: 2190 2434 2190
Size: 244 5739
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4990 809
Size: 507 3674 1802
Fragments arranged by size:
3674 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2192 3189
Size: 54 1425 50 275 18 177 185 997
Cuts at: 3189 3430 4319 5948 8
Size: 241 889 1629 43
Fragments arranged by size:
1629 1425 997 889 275 241 185 177
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3430 1537
Size: 275 1618 4090
Fragments arranged by size:
4090 1618 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3014 3913 1353
Size: 1661 899 3423
Fragments arranged by size:
3423 1661 899
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2111 2219 3016 4570 4944 5090
Size: 1064 138 522 108 797 1554 374 146
Cuts at: 5090 387
Size: 1280
Fragments arranged by size:
1554 1280 1064 797 522 374 146 138
108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2261 2363 2373 2431 2561 2591 2873
Size: 393 629 102 10 58 130 30 282
Cuts at: 2873 3156 3265 4046 4643 5430 5698 1239
Size: 283 109 781 597 787 268 1524
Fragments arranged by size:
1524 787 781 629 597 393 283 282
268 130 109 102 58 30 10
EcoO109I rG'GnC_Cy
Cuts at: 2080 2093 2137 2536 2895 2896 3274 3275 2080
Size: 13 44 399 359 1 378 1 4788
Fragments arranged by size:
4788 399 378 359 44 13 1 1
EcoRI G'AATT_C
Cuts at: 3292 3670 3292
Size: 378 5605
FseI GG_CCGG'CC
Cuts at: 2013 2013
Size: 5983
FspI TGC'GCA
Cuts at: 2474 2612 4489 2474
Size: 138 1877 3968
Fragments arranged by size:
3968 1877 138
HaeII r_GCGC'y
Cuts at: 1678 2830 5130 1678
Size: 1152 2300 2531
Fragments arranged by size:
2531 2300 1152
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2979 3011 4624 4656 4698
Size: 32 135 32 1407 32 1613 32 42
Cuts at: 4698 4730 1373
Size: 32 2626
Fragments arranged by size:
2626 1613 1407 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3327 3573 669
Size: 988 281 1389 246 3079
Fragments arranged by size:
3079 1389 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5983
HpaI GTT'AAC
Cuts at: 3573 3573
Size: 5983
KpnI G_GTAC'C
Cuts at: 3308 3308
Size: 5983
MfeI C'AATT_G
Cuts at: 3582 3582
Size: 5983
MluI A'CGCG_T
Cuts at: 5859 5859
Size: 5983
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2578 5097 5281 1305
Size: 1273 2519 184 2007
Fragments arranged by size:
2519 2007 1273 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2194 10
Size: 54 1425 343 362 3799
Fragments arranged by size:
3799 1425 362 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2639 3941 4300 4459 518
Size: 648 1473 1302 359 159 2042
Fragments arranged by size:
2042 1473 1302 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2143 2212 2239 2409 2478 2773 3097 3399
Size: 537 69 27 170 69 295 324 302
Cuts at: 3399 4076 5224 5469 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 537 324 302 295 245
170 69 69 27
NaeI GCC'GGC
Cuts at: 2011 2011
Size: 5983
NcoI C'CATG_G
Cuts at: 513 1065 2004 2125 2157 3124 3139 513
Size: 552 939 121 32 967 15 3357
Fragments arranged by size:
3357 967 939 552 121 32 15
NdeI CA'TA_TG
Cuts at: 387 2929 387
Size: 2542 3441
NgoMIV G'CCGG_C
Cuts at: 2009 2009
Size: 5983
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5983
NotI GC'GGCC_GC
Cuts at: 3430 3430
Size: 5983
NspI r_CATG'y
Cuts at: 1132 1229 3341 4886 1132
Size: 97 2112 1545 2229
Fragments arranged by size:
2229 2112 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2899 1348
Size: 1551 4432
PciI A'CATG_T
Cuts at: 4882 4882
Size: 5983
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2190 2239 2830 1108
Size: 1082 49 591 4261
Fragments arranged by size:
4261 1082 591 49
PmeI GTTT'AAAC
Cuts at: 3287 3287
Size: 5983
PmlI CAC'GTG
Cuts at: 2740 2740
Size: 5983
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4052 4084 5565 5597 4052
Size: 32 1481 32 4438
Fragments arranged by size:
4438 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2093 2093
Size: 5983
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5983
PsiI TTA'TAA
Cuts at: 3553 3553
Size: 5983
PspOMI G'GGCC_C
Cuts at: 2079 2136 2743 2895 3274 2079
Size: 57 607 152 379 4788
Fragments arranged by size:
4788 607 379 152 57
PstI C_TGCA'G
Cuts at: 830 3335 830
Size: 2505 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5983
PvuII CAG'CTG
Cuts at: 2212 2239 2478 2773 3097 2212
Size: 27 239 295 324 5098
Fragments arranged by size:
5098 324 295 239 27
SalI G'TCGA_C
Cuts at: 1936 3325 1936
Size: 1389 4594
SbfI CC_TGCA'GG
Cuts at: 3335 3335
Size: 5983
ScaI AGT'ACT
Cuts at: 4231 5943 4231
Size: 1712 4271
SfcI C'TryA_G
Cuts at: 826 1045 3198 3331 4466 5147 5338 826
Size: 219 2153 133 1135 681 191 1471
Fragments arranged by size:
2153 1471 1135 681 219 191 133
SfiI GGCCn_nnn'nGGCC
Cuts at: 2144 2516 2144
Size: 372 5611
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5983
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5983
SmaI CCC'GGG
Cuts at: 1872 2078 2753 2819 3058 3310 1872
Size: 206 675 66 239 252 4545
Fragments arranged by size:
4545 675 252 239 206 66
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2843 4087 4988 5250 820
Size: 197 926 900 1244 901 262 1553
Fragments arranged by size:
1553 1244 926 901 900 262 197
SnaBI TAC'GTA
Cuts at: 493 3774 493
Size: 3281 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5983
SphI G_CATG'C
Cuts at: 1132 3341 1132
Size: 2209 3774
SspI AAT'ATT
Cuts at: 5 52 3907 5
Size: 47 3855 2081
Fragments arranged by size:
3855 2081 47
StuI AGG'CCT
Cuts at: 1517 2202 2276 1517
Size: 685 74 5224
Fragments arranged by size:
5224 685 74
StyI C'CwwG_G
Cuts at: 513 1065 2004 2125 2157 3124 3139 3267 3422
Size: 552 939 121 32 967 15 128 155
Cuts at: 3422 513
Size: 3074
Fragments arranged by size:
3074 967 939 552 155 128 121 32
15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4010 4027 4180 4365 4810 325
Size: 1255 2430 17 153 185 445 1498
Fragments arranged by size:
2430 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2310 2856 4229
Size: 274 80 33 51 1443 333 546 1373
Cuts at: 4229 5941 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1373 546 333 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2085 2117 2724 2756 5943 5975 2085
Size: 32 607 32 3187 32 2093
Fragments arranged by size:
3187 2093 607 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5983
XbaI T'CTAG_A
Cuts at: 3319 3319
Size: 5983
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2673 2011
Size: 662 5321
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5983
XmaI C'CCGG_G
Cuts at: 1870 2076 2751 2817 3056 3308 1870
Size: 206 675 66 239 252 4545
Fragments arranged by size:
4545 675 252 239 206 66
XmnI GAAnn'nnTTC
Cuts at: 4112 4112
Size: 5983
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3791 276
Size: 53 83 186 936 2257 2468
Fragments arranged by size:
2468 2257 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I EcoICRI EcoNI EcoRV FalI FspAI KasI NarI
NruI NsiI PacI PfoI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfoI SrfI SwaI