Restriction map for
pFN21AB7203
(Circular) (Six-base) MAPSORT of: pFN21AB7203.seq Check: 4388 from: 1 to: 6574
pFN21AB7203 6574 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4384 278
Size: 53 83 186 936 2848 2468
Fragments arranged by size:
2848 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3917 1937
Size: 1980 4594
Acc65I G'GTAC_C
Cuts at: 3895 3895
Size: 6574
AclI AA'CG_TT
Cuts at: 1563 4701 5074 1563
Size: 3138 373 3063
Fragments arranged by size:
3138 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2224 2716 3364 3644 4637 6021 6289 1632
Size: 592 492 648 280 993 1384 268 1917
Fragments arranged by size:
1917 1384 993 648 592 492 280 268
AfeI AGC'GCT
Cuts at: 3668 3668
Size: 6574
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6377
AflIII A'CryG_T
Cuts at: 1203 5473 6450 1203
Size: 4270 977 1327
Fragments arranged by size:
4270 1327 977
AgeI A'CCGG_T
Cuts at: 2552 5405 2552
Size: 2853 3721
AhdI GACnn_n'nnGTC
Cuts at: 5303 5303
Size: 6574
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6574
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2638 2834 1184
Size: 1454 196 4924
Fragments arranged by size:
4924 1454 196
AlwNI CAG_nnn'CTG
Cuts at: 3511 4317 5889 3511
Size: 806 1572 4196
Fragments arranged by size:
4196 1572 806
ApaI G_GGCC'C
Cuts at: 2665 2765 2853 2665
Size: 100 88 6386
Fragments arranged by size:
6386 100 88
ApaLI G'TGCA_C
Cuts at: 2900 4631 5787 2900
Size: 1731 1156 3687
Fragments arranged by size:
3687 1731 1156
ApoI r'AATT_y
Cuts at: 1493 3883 4109 4261 6370 1493
Size: 2390 226 152 2109 1697
Fragments arranged by size:
2390 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5128 160
Size: 872 4096 1606
Fragments arranged by size:
4096 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6574
AvaI C'yCGr_G
Cuts at: 1870 1943 2061 2348 2852 3899 1870
Size: 73 118 287 504 1047 4545
Fragments arranged by size:
4545 1047 504 287 118 73
AvrII C'CTAG_G
Cuts at: 2590 2590
Size: 6574
BamHI G'GATC_C
Cuts at: 3904 4271 3904
Size: 367 6207
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3352 3514 3895 5350 618
Size: 325 217 627 1565 162 381 1455 1842
Fragments arranged by size:
1842 1565 1455 627 381 325 217 162
BanII G_rGCy'C
Cuts at: 1391 2298 2526 2542 2665 2765 2772 2853 3077
Size: 907 228 16 123 100 7 81 224
Cuts at: 3077 3258 3893 1391
Size: 181 635 4072
Fragments arranged by size:
4072 907 635 228 224 181 123 100
81 16 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3449 928
Size: 971 1550 4053
Fragments arranged by size:
4053 1550 971
BbvCI CC'TCA_GC
Cuts at: 2288 2448 2288
Size: 160 6414
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4765 4799 1903
Size: 34 2828 34 3678
Fragments arranged by size:
3678 2828 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4466 5676 4466
Size: 1210 5364
BclI T'GATC_A
Cuts at: 1548 3600 1548
Size: 2052 4522
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2726 5185 136
Size: 107 122 71 631 605 1054 2459 1525
Fragments arranged by size:
2459 1525 1054 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6569 6569
Size: 6574
BlpI GC'TnA_GC
Cuts at: 3991 3991
Size: 6574
Bme1580I G_kGCm'C
Cuts at: 1790 2445 2665 2765 2853 2904 3201 3519 4635
Size: 655 220 100 88 51 297 318 1116
Cuts at: 4635 5791 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1116 655 318 297 220 100
88 51
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6574
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3471 3743 4305
Size: 416 53 83 399 24 2054 272 562
Cuts at: 4305 5263 442
Size: 958 1753
Fragments arranged by size:
2054 1753 958 562 416 399 272 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6574
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2952 2952
Size: 6574
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2173 2222 2279 2476 2486 2567 2859 2884
Size: 934 49 57 197 10 81 292 25
Cuts at: 2884 2899 5234 1239
Size: 15 2335 2579
Fragments arranged by size:
2579 2335 934 292 197 81 57 49
25 15 10
Bpu10I CC'TnA_GC
Cuts at: 2288 2384 2448 2288
Size: 96 64 6414
Fragments arranged by size:
6414 96 64
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3245 4699 5564 5862 3245
Size: 1454 865 298 3957
Fragments arranged by size:
3957 1454 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2543 5237 882
Size: 617 1044 2694 2219
Fragments arranged by size:
2694 2219 1044 617
BsaAI yAC'GTr
Cuts at: 493 1204 2711 4365 493
Size: 711 1507 1654 2702
Fragments arranged by size:
2702 1654 1507 711
BsaBI GATnn'nnATC
Cuts at: 6568 6568
Size: 6574
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2168 2326 2391 4381
Size: 53 83 186 936 635 158 65 1990
Cuts at: 4381 4763 5398 275
Size: 382 635 1451
Fragments arranged by size:
1990 1451 936 635 635 382 186 158
83 65 53
BsaWI w'CCGG_w
Cuts at: 2552 2972 5007 5405 5679 5826 6237 2552
Size: 420 2035 398 274 147 411 2889
Fragments arranged by size:
2889 2035 420 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2252 2282 2954 2984 3677
Size: 30 165 30 849 30 672 30 693
Cuts at: 3677 3707 3749 3779 1178
Size: 30 42 30 3973
Fragments arranged by size:
3973 849 693 672 165 42 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2881 3227 3773 1186
Size: 1695 346 546 3987
Fragments arranged by size:
3987 1695 546 346
BseYI C'CCAG_C
Cuts at: 2658 2941 3200 5777 6273 2658
Size: 283 259 2577 496 2959
Fragments arranged by size:
2959 2577 496 283 259
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3452 3524 1163
Size: 573 1716 72 4213
Fragments arranged by size:
4213 1716 573 72
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4024 4785 5813 1540
Size: 275 186 2023 761 1028 2301
Fragments arranged by size:
2301 2023 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2298 2526 2772 2904 3085 3231 3258 3658 4635
Size: 228 246 132 181 146 27 400 977
Cuts at: 4635 4720 5791 2298
Size: 85 1071 3081
Fragments arranged by size:
3081 1071 977 400 246 228 181 146
132 85 27
BsmI GAATG_Cn'
Cuts at: 4085 4178 4085
Size: 93 6481
BsmBI CGTCTCn'nnnn_
Cuts at: 2536 2536
Size: 6574
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2298 2445 2526 2542 2665 2765 2772
Size: 399 508 147 81 16 123 100 7
Cuts at: 2772 2853 2904 3077 3085 3201 3231 3258 3519
Size: 81 51 173 8 116 30 27 261
Cuts at: 3519 3658 3893 4635 4720 5791 1391
Size: 139 235 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 508 399 261 235 173
147 139 123 116 100 85 81 81
51 30 27 16 8 7
BspEI T'CCGG_A
Cuts at: 2972 6237 2972
Size: 3265 3309
BspHI T'CATG_A
Cuts at: 4463 6193 4463
Size: 1730 4844
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2424 3929 844
Size: 1580 1505 3489
Fragments arranged by size:
3489 1580 1505
BsrBI CCG'CTC
Cuts at: 1394 1823 2206 4461 1394
Size: 429 383 2255 3507
Fragments arranged by size:
3507 2255 429 383
BsrDI GCAATG_nn'
Cuts at: 65 1248 3618 5069 5243 65
Size: 1183 2370 1451 174 1396
Fragments arranged by size:
2370 1451 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2552 5218 5405 1700
Size: 110 742 2666 187 2869
Fragments arranged by size:
2869 2666 742 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6574
BssHII G'CGCG_C
Cuts at: 1925 2456 1925
Size: 531 6043
BssSI C'ACGA_G
Cuts at: 2926 4634 5646 2926
Size: 1708 1012 3854
Fragments arranged by size:
3854 1708 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3415 794
Size: 2621 3953
BstBI TT'CG_AA
Cuts at: 5378 5378
Size: 6574
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6574
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 3487 3556 2011
Size: 1476 69 5029
Fragments arranged by size:
5029 1476 69
BstYI r'GATC_y
Cuts at: 1972 3904 4271 4656 4673 6125 6211 6223 6569
Size: 1932 367 385 17 1452 86 12 346
Cuts at: 6569 1972
Size: 1977
Fragments arranged by size:
1977 1932 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6022
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5835
Fragments arranged by size:
5835 683 56
BtsI GCAGTG_nn'
Cuts at: 2816 2961 3536 3658 4090 4884 4904 2816
Size: 145 575 122 432 794 20 4486
Fragments arranged by size:
4486 794 575 432 145 122 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6574
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6539
DraI TTT'AAA
Cuts at: 3878 3878
Size: 6574
DraIII CAC_nnn'GTG
Cuts at: 3334 3334
Size: 6574
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5581 809
Size: 507 4265 1802
Fragments arranged by size:
4265 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2211 2361 2569
Size: 54 1425 50 275 18 381 150 208
Cuts at: 2569 2718 2784 3141 3180 3609 4021 4910 6539
Size: 149 66 357 39 429 412 889 1629
Cuts at: 6539 8
Size: 43
Fragments arranged by size:
1629 1425 889 429 412 381 357 275
208 150 149 66 54 50 43 39
18
EagI C'GGCC_G
Cuts at: 1537 1812 4021 1537
Size: 275 2209 4090
Fragments arranged by size:
4090 2209 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3663 4504 1353
Size: 2310 841 3423
Fragments arranged by size:
3423 2310 841
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3120 5161 5535 5681 387
Size: 1064 138 1531 2041 374 146 1280
Fragments arranged by size:
2041 1531 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2296 2524 2770 3256 2296
Size: 228 246 486 5614
Fragments arranged by size:
5614 486 246 228
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2173 2222 2224 2279 2476 2486 2567
Size: 393 541 49 2 55 197 10 81
Cuts at: 2567 2716 2859 2884 2899 3364 3644 4637 5234
Size: 149 143 25 15 465 280 993 597
Cuts at: 5234 6021 6289 1239
Size: 787 268 1524
Fragments arranged by size:
1524 993 787 597 541 465 393 280
268 197 149 143 81 55 49 25
15 10 2
EcoO109I rG'GnC_Cy
Cuts at: 2244 2317 2587 2662 2761 2796 2883 3275 3508
Size: 73 270 75 99 35 87 392 233
Cuts at: 3508 2244
Size: 5310
Fragments arranged by size:
5310 392 270 233 99 87 75 73
35
EcoRI G'AATT_C
Cuts at: 3883 4261 3883
Size: 378 6196
FspI TGC'GCA
Cuts at: 5080 5080
Size: 6574
HaeII r_GCGC'y
Cuts at: 1678 2384 3670 5721 1678
Size: 706 1286 2051 2531
Fragments arranged by size:
2531 2051 1286 706
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2967 2999 3441 3473 3749
Size: 32 135 32 1395 32 442 32 276
Cuts at: 3749 3781 5215 5247 5289 5321 1373
Size: 32 1434 32 42 32 2626
Fragments arranged by size:
2626 1434 1395 442 276 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3721 3918 4164 669
Size: 988 281 1783 197 246 3079
Fragments arranged by size:
3079 1783 988 281 246 197
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6574
HpaI GTT'AAC
Cuts at: 4164 4164
Size: 6574
KpnI G_GTAC'C
Cuts at: 3899 3899
Size: 6574
MfeI C'AATT_G
Cuts at: 4173 4173
Size: 6574
MluI A'CGCG_T
Cuts at: 6450 6450
Size: 6574
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5688 5872 1305
Size: 4383 184 2007
Fragments arranged by size:
4383 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2786 3143 3182 3611 10
Size: 54 1425 343 954 357 39 429 2973
Fragments arranged by size:
2973 1425 954 429 357 343 54 39
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 4532 4891 5050 518
Size: 648 843 2523 359 159 2042
Fragments arranged by size:
2523 2042 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2088 2266 2447 2503 3173 3990 4667 5815
Size: 482 178 181 56 670 817 677 1148
Cuts at: 5815 6060 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 817 677 670 482 245 181
178 56
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6022
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6574
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6574
NotI GC'GGCC_GC
Cuts at: 4021 4021
Size: 6574
NspI r_CATG'y
Cuts at: 1132 1229 2026 2619 3152 3418 3932 5477 1132
Size: 97 797 593 533 266 514 1545 2229
Fragments arranged by size:
2229 1545 797 593 533 514 266 97
PasI CC'CwG_GG
Cuts at: 1348 2248 2373 2610 2846 3247 1348
Size: 900 125 237 236 401 4675
Fragments arranged by size:
4675 900 401 237 236 125
PciI A'CATG_T
Cuts at: 5473 5473
Size: 6574
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2104 1108
Size: 996 5578
PfoI T'CCnGG_A
Cuts at: 2463 2776 2463
Size: 313 6261
PmeI GTTT'AAAC
Cuts at: 3878 3878
Size: 6574
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4643 4675 6156 6188 4643
Size: 32 1481 32 5029
Fragments arranged by size:
5029 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2587 2796 3275 2587
Size: 209 479 5886
Fragments arranged by size:
5886 479 209
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6574
PsiI TTA'TAA
Cuts at: 4144 4144
Size: 6574
PspOMI G'GGCC_C
Cuts at: 2661 2761 2849 2661
Size: 100 88 6386
Fragments arranged by size:
6386 100 88
PstI C_TGCA'G
Cuts at: 830 2093 2421 2956 3653 3926 830
Size: 1263 328 535 697 273 3478
Fragments arranged by size:
3478 1263 697 535 328 273
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6574
PvuII CAG'CTG
Cuts at: 2088 2266 2503 2088
Size: 178 237 6159
Fragments arranged by size:
6159 237 178
SacI G_AGCT'C
Cuts at: 2298 2526 2772 3258 2298
Size: 228 246 486 5614
Fragments arranged by size:
5614 486 246 228
SalI G'TCGA_C
Cuts at: 1936 3916 1936
Size: 1980 4594
SapI GCTCTTCn'nnn_
Cuts at: 3663 3663
Size: 6574
SbfI CC_TGCA'GG
Cuts at: 2421 3926 2421
Size: 1505 5069
ScaI AGT'ACT
Cuts at: 3866 4822 6534 3866
Size: 956 1712 3906
Fragments arranged by size:
3906 1712 956
SexAI A'CCwGG_T
Cuts at: 2637 2637
Size: 6574
SfcI C'TryA_G
Cuts at: 826 1045 2089 2417 2597 2952 3649 3739 3922
Size: 219 1044 328 180 355 697 90 183
Cuts at: 3922 5057 5738 5929 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1044 697 681 355 328 219
191 183 180 90
SfiI GGCCn_nnn'nGGCC
Cuts at: 2726 2726
Size: 6574
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6574
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6574
SmaI CCC'GGG
Cuts at: 1872 2063 2350 3901 1872
Size: 191 287 1551 4545
Fragments arranged by size:
4545 1551 287 191
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3224 4678 5579 5841 820
Size: 197 926 1281 1454 901 262 1553
Fragments arranged by size:
1553 1454 1281 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4365 493
Size: 3872 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6574
SphI G_CATG'C
Cuts at: 1132 2026 3418 3932 1132
Size: 894 1392 514 3774
Fragments arranged by size:
3774 1392 894 514
SspI AAT'ATT
Cuts at: 5 52 4498 5
Size: 47 4446 2081
Fragments arranged by size:
4446 2081 47
StuI AGG'CCT
Cuts at: 1517 2237 3830 1517
Size: 720 1593 4261
Fragments arranged by size:
4261 1593 720
StyI C'CwwG_G
Cuts at: 513 1065 2590 3339 4013 513
Size: 552 1525 749 674 3074
Fragments arranged by size:
3074 1525 749 674 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2096 2795 4601 4618 4771 4956 5401
Size: 1255 516 699 1806 17 153 185 445
Cuts at: 5401 325
Size: 1498
Fragments arranged by size:
1806 1498 1255 699 516 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3864 4820 6532
Size: 274 80 33 51 1443 1887 956 1712
Cuts at: 6532 96
Size: 138
Fragments arranged by size:
1887 1712 1443 956 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2252 2284 2954 2986 3180 3212 3320 3352 6534
Size: 32 670 32 194 32 108 32 3182
Cuts at: 6534 6566 2252
Size: 32 2260
Fragments arranged by size:
3182 2260 670 194 108 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6574
XbaI T'CTAG_A
Cuts at: 3910 3910
Size: 6574
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2197 3001 3084 3553 3556 2197
Size: 804 83 469 3 5215
Fragments arranged by size:
5215 804 469 83 3
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6574
XmaI C'CCGG_G
Cuts at: 1870 2061 2348 3899 1870
Size: 191 287 1551 4545
Fragments arranged by size:
4545 1551 287 191
XmnI GAAnn'nnTTC
Cuts at: 4703 4703
Size: 6574
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4382 276
Size: 53 83 186 936 2848 2468
Fragments arranged by size:
2848 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SexAI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BbeI BfrBI BsiWI BstZ17I
Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SacII SanDI SfoI SrfI SwaI