Restriction map for
pFN21AB7202
(Circular) (Six-base) MAPSORT of: pFN21AB7202.seq Check: 8098 from: 1 to: 5428
pFN21AB7202 5428 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3238 278
Size: 53 83 186 936 1702 2468
Fragments arranged by size:
2468 1702 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2771 1937
Size: 834 4594
Acc65I G'GTAC_C
Cuts at: 2749 2749
Size: 5428
AclI AA'CG_TT
Cuts at: 1563 3555 3928 1563
Size: 1992 373 3063
Fragments arranged by size:
3063 1992 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3491 4875 5143 1632
Size: 1859 1384 268 1917
Fragments arranged by size:
1917 1859 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5231
AflIII A'CryG_T
Cuts at: 1203 4327 5304 1203
Size: 3124 977 1327
Fragments arranged by size:
3124 1327 977
AgeI A'CCGG_T
Cuts at: 4259 4259
Size: 5428
AhdI GACnn_n'nnGTC
Cuts at: 4157 4157
Size: 5428
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5428
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5428
AlwNI CAG_nnn'CTG
Cuts at: 2434 3171 4743 2434
Size: 737 1572 3119
Fragments arranged by size:
3119 1572 737
ApaI G_GGCC'C
Cuts at: 2050 2050
Size: 5428
ApaLI G'TGCA_C
Cuts at: 3485 4641 3485
Size: 1156 4272
ApoI r'AATT_y
Cuts at: 1493 2737 2963 3115 5224 1493
Size: 1244 226 152 2109 1697
Fragments arranged by size:
2109 1697 1244 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3982 160
Size: 872 2950 1606
Fragments arranged by size:
2950 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5428
AvaI C'yCGr_G
Cuts at: 1870 1943 2041 2311 2399 2753 1870
Size: 73 98 270 88 354 4545
Fragments arranged by size:
4545 354 270 98 88 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2257 2290 2257
Size: 33 5395
BamHI G'GATC_C
Cuts at: 2758 3125 2758
Size: 367 5061
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2143 2505 2749 4204 618
Size: 325 217 627 356 362 244 1455 1842
Fragments arranged by size:
1842 1455 627 362 356 325 244 217
BanII G_rGCy'C
Cuts at: 1391 2050 2292 2568 2595 2747 1391
Size: 659 242 276 27 152 4072
Fragments arranged by size:
4072 659 276 242 152 27
BbeI G_GCGC'C
Cuts at: 2509 2509
Size: 5428
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4457
BbvCI CC'TCA_GC
Cuts at: 2660 2660
Size: 5428
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2507 2541 3619 3653 1903
Size: 34 570 34 1078 34 3678
Fragments arranged by size:
3678 1078 570 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2285 2433 3320 4530 2285
Size: 148 887 1210 3183
Fragments arranged by size:
3183 1210 887 148
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5428
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2572 2674 4039
Size: 107 122 71 631 605 900 102 1365
Cuts at: 4039 136
Size: 1525
Fragments arranged by size:
1525 1365 900 631 605 122 107 102
71
BglII A'GATC_T
Cuts at: 5423 5423
Size: 5428
BlpI GC'TnA_GC
Cuts at: 2845 2845
Size: 5428
Bme1580I G_kGCm'C
Cuts at: 1790 2050 2146 2318 3489 4645 1790
Size: 260 96 172 1171 1156 2573
Fragments arranged by size:
2573 1171 1156 260 172 96
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5428
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2480 3159 4117
Size: 416 53 83 399 24 1063 679 958
Cuts at: 4117 442
Size: 1753
Fragments arranged by size:
1753 1063 958 679 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5428
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2219 2306 4088 1239
Size: 980 87 1782 2579
Fragments arranged by size:
2579 1782 980 87
Bpu10I CC'TnA_GC
Cuts at: 2660 2660
Size: 5428
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2317 3553 4418 4716 2317
Size: 1236 865 298 3029
Fragments arranged by size:
3029 1236 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4091 882
Size: 617 2592 2219
Fragments arranged by size:
2592 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2368 3219 493
Size: 711 1164 851 2702
Fragments arranged by size:
2702 1164 851 711
BsaBI GATnn'nnATC
Cuts at: 5422 5422
Size: 5428
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2182 2506 3235 3617
Size: 53 83 186 936 649 324 729 382
Cuts at: 3617 4252 275
Size: 635 1451
Fragments arranged by size:
1451 936 729 649 635 382 324 186
83 53
BsaWI w'CCGG_w
Cuts at: 3861 4259 4533 4680 5091 3861
Size: 398 274 147 411 4198
Fragments arranged by size:
4198 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2212 2242 1178
Size: 30 165 30 809 30 4364
Fragments arranged by size:
4364 809 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2127 2282 1186
Size: 941 155 4332
Fragments arranged by size:
4332 941 155
BseYI C'CCAG_C
Cuts at: 2133 2147 2371 2427 2446 2453 4631 5127 2133
Size: 14 224 56 19 7 2178 496 2434
Fragments arranged by size:
2434 2178 496 224 56 19 14 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2483 1163
Size: 573 747 4108
Fragments arranged by size:
4108 747 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2080 2189 2251 2878 3639 4667
Size: 275 186 79 109 62 627 761 1028
Cuts at: 4667 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 627 275 186 109 79
62
BsiHKAI G_wGCw'C
Cuts at: 2292 2466 3489 3574 4645 2292
Size: 174 1023 85 1071 3075
Fragments arranged by size:
3075 1071 1023 174 85
BsmI GAATG_Cn'
Cuts at: 2939 3032 2939
Size: 93 5335
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2050 2146 2292 2318 2466 2568 2595
Size: 399 260 96 146 26 148 102 27
Cuts at: 2595 2747 3489 3574 4645 1391
Size: 152 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 260 152 148 146
102 96 85 27 26
BspEI T'CCGG_A
Cuts at: 5091 5091
Size: 5428
BspHI T'CATG_A
Cuts at: 3317 5047 3317
Size: 1730 3698
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2783 844
Size: 1939 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2211 3315 1394
Size: 429 388 1104 3507
Fragments arranged by size:
3507 1104 429 388
BsrDI GCAATG_nn'
Cuts at: 65 1248 3923 4097 65
Size: 1183 2675 174 1396
Fragments arranged by size:
2675 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2673 4072 4259 1700
Size: 110 863 1399 187 2869
Fragments arranged by size:
2869 1399 863 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5428
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5428
BssSI C'ACGA_G
Cuts at: 2339 3488 4500 2339
Size: 1149 1012 3267
Fragments arranged by size:
3267 1149 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5428
BstBI TT'CG_AA
Cuts at: 4232 4232
Size: 5428
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5428
BstYI r'GATC_y
Cuts at: 1972 2641 2758 3125 3510 3527 4979 5065 5077
Size: 669 117 367 385 17 1452 86 12
Cuts at: 5077 5423 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 669 385 367 346 117 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2410 513
Size: 552 939 406 3531
Fragments arranged by size:
3531 939 552 406
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4689
Fragments arranged by size:
4689 683 56
BtsI GCAGTG_nn'
Cuts at: 2944 3738 3758 2944
Size: 794 20 4614
Fragments arranged by size:
4614 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5428
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5393
DraI TTT'AAA
Cuts at: 2732 2732
Size: 5428
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4435 809
Size: 507 3119 1802
Fragments arranged by size:
3119 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2077 2248 2875
Size: 54 1425 50 275 18 247 171 627
Cuts at: 2875 3764 5393 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 627 275 247 171 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2077 2248 2875 1537
Size: 275 265 171 627 4090
Fragments arranged by size:
4090 627 275 265 171
EarI CTCTTCn'nnn_
Cuts at: 1353 2209 2583 3358 1353
Size: 856 374 775 3423
Fragments arranged by size:
3423 856 775 374
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4015 4389 4535 387
Size: 1064 138 2426 374 146 1280
Fragments arranged by size:
2426 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2290 2290
Size: 5428
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2219 2306 3491 4088 4875 5143 1239
Size: 393 587 87 1185 597 787 268 1524
Fragments arranged by size:
1524 1185 787 597 587 393 268 87
EcoNI CCTnn'n_nnAGG
Cuts at: 2615 2615
Size: 5428
EcoO109I rG'GnC_Cy
Cuts at: 2046 2167 2214 2382 2395 2608 2667 2046
Size: 121 47 168 13 213 59 4807
Fragments arranged by size:
4807 213 168 121 59 47 13
EcoRI G'AATT_C
Cuts at: 2737 3115 2737
Size: 378 5050
FspI TGC'GCA
Cuts at: 3934 3934
Size: 5428
HaeII r_GCGC'y
Cuts at: 1678 2454 2509 4575 1678
Size: 776 55 2066 2531
Fragments arranged by size:
2531 2066 776 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4069 4101 4143 4175 1373
Size: 32 135 32 2497 32 42 32 2626
Fragments arranged by size:
2626 2497 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2772 3018 669
Size: 988 281 834 246 3079
Fragments arranged by size:
3079 988 834 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5428
HpaI GTT'AAC
Cuts at: 3018 3018
Size: 5428
KasI G'GCGC_C
Cuts at: 2505 2505
Size: 5428
KpnI G_GTAC'C
Cuts at: 2753 2753
Size: 5428
MfeI C'AATT_G
Cuts at: 3027 3027
Size: 5428
MluI A'CGCG_T
Cuts at: 5304 5304
Size: 5428
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4542 4726 1305
Size: 3237 184 2007
Fragments arranged by size:
3237 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3606
Fragments arranged by size:
3606 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3386 3745 3904 518
Size: 648 843 1377 359 159 2042
Fragments arranged by size:
2042 1377 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2023 2032 2133 2443 2687 2844 3521 4669
Size: 417 9 101 310 244 157 677 1148
Cuts at: 4669 4914 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 417 310 245 244 157
101 9
NaeI GCC'GGC
Cuts at: 2675 2675
Size: 5428
NarI GG'CG_CC
Cuts at: 2506 2506
Size: 5428
NcoI C'CATG_G
Cuts at: 513 1065 2004 2410 513
Size: 552 939 406 3531
Fragments arranged by size:
3531 939 552 406
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5428
NgoMIV G'CCGG_C
Cuts at: 2673 2673
Size: 5428
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5428
NotI GC'GGCC_GC
Cuts at: 2875 2875
Size: 5428
NspI r_CATG'y
Cuts at: 1132 1229 2786 4331 1132
Size: 97 1557 1545 2229
Fragments arranged by size:
2229 1557 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2060 2084 1348
Size: 712 24 4692
Fragments arranged by size:
4692 712 24
PciI A'CATG_T
Cuts at: 4327 4327
Size: 5428
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2133 1108
Size: 1025 4403
PmeI GTTT'AAAC
Cuts at: 2732 2732
Size: 5428
PmlI CAC'GTG
Cuts at: 2368 2368
Size: 5428
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3497 3529 5010 5042 3497
Size: 32 1481 32 3883
Fragments arranged by size:
3883 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2214 2382 2608 2214
Size: 168 226 5034
Fragments arranged by size:
5034 226 168
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5428
PsiI TTA'TAA
Cuts at: 2998 2998
Size: 5428
PspOMI G'GGCC_C
Cuts at: 2046 2046
Size: 5428
PstI C_TGCA'G
Cuts at: 830 2022 2780 830
Size: 1192 758 3478
Fragments arranged by size:
3478 1192 758
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5428
PvuII CAG'CTG
Cuts at: 2023 2023
Size: 5428
SacI G_AGCT'C
Cuts at: 2292 2292
Size: 5428
SalI G'TCGA_C
Cuts at: 1936 2770 1936
Size: 834 4594
SanDI GG'GwC_CC
Cuts at: 2214 2214
Size: 5428
SapI GCTCTTCn'nnn_
Cuts at: 2583 2583
Size: 5428
SbfI CC_TGCA'GG
Cuts at: 2780 2780
Size: 5428
ScaI AGT'ACT
Cuts at: 3676 5388 3676
Size: 1712 3716
SfcI C'TryA_G
Cuts at: 826 1045 2018 2776 3911 4592 4783 826
Size: 219 973 758 1135 681 191 1471
Fragments arranged by size:
1471 1135 973 758 681 219 191
SfoI GGC'GCC
Cuts at: 2507 2507
Size: 5428
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5428
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5428
SmaI CCC'GGG
Cuts at: 1872 2401 2755 1872
Size: 529 354 4545
Fragments arranged by size:
4545 529 354
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2041 2296 3532 4433 4695 820
Size: 197 926 98 255 1236 901 262 1553
Fragments arranged by size:
1553 1236 926 901 262 255 197 98
SnaBI TAC'GTA
Cuts at: 493 3219 493
Size: 2726 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5428
SphI G_CATG'C
Cuts at: 1132 2786 1132
Size: 1654 3774
SspI AAT'ATT
Cuts at: 5 52 3352 5
Size: 47 3300 2081
Fragments arranged by size:
3300 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5428
StyI C'CwwG_G
Cuts at: 513 1065 2004 2050 2410 2867 513
Size: 552 939 46 360 457 3074
Fragments arranged by size:
3074 939 552 457 360 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2161 2203 3455 3472 3625 3810 4255
Size: 1255 581 42 1252 17 153 185 445
Cuts at: 4255 325
Size: 1498
Fragments arranged by size:
1498 1255 1252 581 445 185 153 42
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3674 5386 96
Size: 274 80 33 51 1443 1697 1712 138
Fragments arranged by size:
1712 1697 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2136 2168 2212 2244 5388 5420 2136
Size: 32 44 32 3144 32 2144
Fragments arranged by size:
3144 2144 44 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5428
XbaI T'CTAG_A
Cuts at: 2764 2764
Size: 5428
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2057 2057
Size: 5428
XhoI C'TCGA_G
Cuts at: 1943 2041 1943
Size: 98 5330
XmaI C'CCGG_G
Cuts at: 1870 2399 2753 1870
Size: 529 354 4545
Fragments arranged by size:
4545 529 354
XmnI GAAnn'nnTTC
Cuts at: 3557 3557
Size: 5428
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3236 276
Size: 53 83 186 936 1702 2468
Fragments arranged by size:
2468 1702 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BfrBI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI
FspAI NruI NsiI PacI PfoI PspXI PsrI RsrII
SacII SexAI SfiI SrfI SwaI