Restriction map for
pFN21AB7191
(Circular) (Six-base) MAPSORT of: pFN21AB7191.seq Check: 6857 from: 1 to: 6328
pFN21AB7191 6328 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4138 278
Size: 53 83 186 936 2602 2468
Fragments arranged by size:
2602 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3671 1937
Size: 1734 4594
Acc65I G'GTAC_C
Cuts at: 3649 3649
Size: 6328
AclI AA'CG_TT
Cuts at: 1563 2355 4455 4828 1563
Size: 792 2100 373 3063
Fragments arranged by size:
3063 2100 792 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4391 5775 6043 1632
Size: 2759 1384 268 1917
Fragments arranged by size:
2759 1917 1384 268
AfeI AGC'GCT
Cuts at: 2977 2977
Size: 6328
AflII C'TTAA_G
Cuts at: 820 1017 3067 820
Size: 197 2050 4081
Fragments arranged by size:
4081 2050 197
AflIII A'CryG_T
Cuts at: 1203 5227 6204 1203
Size: 4024 977 1327
Fragments arranged by size:
4024 1327 977
AgeI A'CCGG_T
Cuts at: 5159 5159
Size: 6328
AhdI GACnn_n'nnGTC
Cuts at: 2566 5057 2566
Size: 2491 3837
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6328
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3017 1184
Size: 1833 4495
AlwNI CAG_nnn'CTG
Cuts at: 2303 2651 4071 5643 2303
Size: 348 1420 1572 2988
Fragments arranged by size:
2988 1572 1420 348
ApaLI G'TGCA_C
Cuts at: 4385 5541 4385
Size: 1156 5172
ApoI r'AATT_y
Cuts at: 1493 2220 2410 3637 3863 4015 6124 1493
Size: 727 190 1227 226 152 2109 1697
Fragments arranged by size:
2109 1697 1227 727 226 190 152
AseI AT'TA_AT
Cuts at: 160 1032 2699 4882 160
Size: 872 1667 2183 1606
Fragments arranged by size:
2183 1667 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6328
AvaI C'yCGr_G
Cuts at: 1870 1943 3653 1870
Size: 73 1710 4545
Fragments arranged by size:
4545 1710 73
BamHI G'GATC_C
Cuts at: 3658 4025 3658
Size: 367 5961
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3649 5104 618
Size: 325 217 627 1862 1455 1842
Fragments arranged by size:
1862 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3572 3647 1391
Size: 2181 75 4072
Fragments arranged by size:
4072 2181 75
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2521 928
Size: 971 622 4735
Fragments arranged by size:
4735 971 622
BbvCI CC'TCA_GC
Cuts at: 3554 3554
Size: 6328
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4519 4553 1903
Size: 34 2582 34 3678
Fragments arranged by size:
3678 2582 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4220 5430 4220
Size: 1210 5118
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6328
BfrBI ATG'CAT
Cuts at: 2737 2737
Size: 6328
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4939 136
Size: 107 122 71 631 605 3267 1525
Fragments arranged by size:
3267 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2255 3561 6323 2255
Size: 1306 2762 2260
Fragments arranged by size:
2762 2260 1306
BlpI GC'TnA_GC
Cuts at: 3571 3745 3571
Size: 174 6154
Bme1580I G_kGCm'C
Cuts at: 1790 4389 5545 1790
Size: 2599 1156 2573
Fragments arranged by size:
2599 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6328
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4059 5017 442
Size: 416 53 83 399 24 2642 958 1753
Fragments arranged by size:
2642 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6328
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3472 3472
Size: 6328
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2280 3586 4988 1239
Size: 1041 1306 1402 2579
Fragments arranged by size:
2579 1402 1306 1041
Bpu10I CC'TnA_GC
Cuts at: 3554 3554
Size: 6328
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2504 4453 5318 5616 2504
Size: 1949 865 298 3216
Fragments arranged by size:
3216 1949 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2417 3397 4991 882
Size: 617 918 980 1594 2219
Fragments arranged by size:
2219 1594 980 918 617
BsaAI yAC'GTr
Cuts at: 493 1204 4119 493
Size: 711 2915 2702
Fragments arranged by size:
2915 2702 711
BsaBI GATnn'nnATC
Cuts at: 6322 6322
Size: 6328
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4135 4517 5152 275
Size: 53 83 186 936 2602 382 635 1451
Fragments arranged by size:
2602 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4761 5159 5433 5580 5991 4761
Size: 398 274 147 411 5098
Fragments arranged by size:
5098 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6103
Fragments arranged by size:
6103 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2543 1186
Size: 1357 4971
BseYI C'CCAG_C
Cuts at: 3347 5531 6027 3347
Size: 2184 496 3648
Fragments arranged by size:
3648 2184 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2601 3033 1163
Size: 573 865 432 4458
Fragments arranged by size:
4458 865 573 432
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3778 4539 5567 1540
Size: 275 186 1777 761 1028 2301
Fragments arranged by size:
2301 1777 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3572 4389 4474 5545 3572
Size: 817 85 1071 4355
Fragments arranged by size:
4355 1071 817 85
BsmI GAATG_Cn'
Cuts at: 3839 3932 3839
Size: 93 6235
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3572 3647 4389 4474 5545 1391
Size: 399 1782 75 742 85 1071 2174
Fragments arranged by size:
2174 1782 1071 742 399 85 75
BspEI T'CCGG_A
Cuts at: 5991 5991
Size: 6328
BspHI T'CATG_A
Cuts at: 2136 4217 5947 2136
Size: 2081 1730 2517
Fragments arranged by size:
2517 2081 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3683 844
Size: 2839 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4215 1394
Size: 429 2392 3507
Fragments arranged by size:
3507 2392 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4823 4997 65
Size: 1183 3575 174 1396
Fragments arranged by size:
3575 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4972 5159 1700
Size: 110 3162 187 2869
Fragments arranged by size:
3162 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2155 96
Size: 2059 4269
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6328
BssSI C'ACGA_G
Cuts at: 2999 3218 4388 5400 2999
Size: 219 1170 1012 3927
Fragments arranged by size:
3927 1170 1012 219
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6328
BstBI TT'CG_AA
Cuts at: 5132 5132
Size: 6328
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6328
BstXI CCAn_nnnn'nTGG
Cuts at: 3313 3388 3313
Size: 75 6253
BstYI r'GATC_y
Cuts at: 1972 2255 3561 3658 4025 4410 4427 5879 5965
Size: 283 1306 97 367 385 17 1452 86
Cuts at: 5965 5977 6323 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1306 385 367 346 283 97
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4837
Fragments arranged by size:
4837 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5589
Fragments arranged by size:
5589 683 56
BtsI GCAGTG_nn'
Cuts at: 3844 4638 4658 3844
Size: 794 20 5514
Fragments arranged by size:
5514 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6328
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6293
DraI TTT'AAA
Cuts at: 3145 3632 3145
Size: 487 5841
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2525 5335 809
Size: 507 1209 2810 1802
Fragments arranged by size:
2810 1802 1209 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2661 3775 4664
Size: 54 1425 50 275 18 831 1114 889
Cuts at: 4664 6293 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1114 889 831 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3775 1537
Size: 275 1963 4090
Fragments arranged by size:
4090 1963 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4258 1353
Size: 2905 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4915 5289 5435 387
Size: 1064 138 3326 374 146 1280
Fragments arranged by size:
3326 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3570 3570
Size: 6328
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2280 3586 4391 4988 5775 6043 1239
Size: 393 648 1306 805 597 787 268 1524
Fragments arranged by size:
1524 1306 805 787 648 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2560 3490 2560
Size: 930 5398
EcoRI G'AATT_C
Cuts at: 3637 4015 3637
Size: 378 5950
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2265 2836 2265
Size: 571 5757
FspI TGC'GCA
Cuts at: 4834 4834
Size: 6328
HaeII r_GCGC'y
Cuts at: 1678 2979 5475 1678
Size: 1301 2496 2531
Fragments arranged by size:
2531 2496 1301
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2552 2584 4969 5001 5043
Size: 32 135 32 980 32 2385 32 42
Cuts at: 5043 5075 1373
Size: 32 2626
Fragments arranged by size:
2626 2385 980 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2041 3672 3918 669
Size: 988 281 103 1631 246 3079
Fragments arranged by size:
3079 1631 988 281 246 103
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6328
HpaI GTT'AAC
Cuts at: 3918 3918
Size: 6328
KpnI G_GTAC'C
Cuts at: 3653 3653
Size: 6328
MfeI C'AATT_G
Cuts at: 3468 3927 3468
Size: 459 5869
MluI A'CGCG_T
Cuts at: 6204 6204
Size: 6328
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2409 3495 3501 5442 5626 1305
Size: 1104 1086 6 1941 184 2007
Fragments arranged by size:
2007 1941 1104 1086 184 6
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2663 10
Size: 54 1425 343 831 3675
Fragments arranged by size:
3675 1425 831 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2577 2867 4286 4645 4804 518
Size: 648 1411 290 1419 359 159 2042
Fragments arranged by size:
2042 1419 1411 648 359 290 159
MspA1I CmG'CkG
Cuts at: 1606 3017 3744 4421 5569 5814 1606
Size: 1411 727 677 1148 245 2120
Fragments arranged by size:
2120 1411 1148 727 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4837
Fragments arranged by size:
4837 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6328
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6328
NotI GC'GGCC_GC
Cuts at: 3775 3775
Size: 6328
NsiI A_TGCA'T
Cuts at: 2739 2739
Size: 6328
NspI r_CATG'y
Cuts at: 1132 1229 2737 3378 3686 5231 1132
Size: 97 1508 641 308 1545 2229
Fragments arranged by size:
2229 1545 1508 641 308 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6328
PciI A'CATG_T
Cuts at: 5227 5227
Size: 6328
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6328
PfoI T'CCnGG_A
Cuts at: 2556 2556
Size: 6328
PmeI GTTT'AAAC
Cuts at: 3632 3632
Size: 6328
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4397 4429 5910 5942 4397
Size: 32 1481 32 4783
Fragments arranged by size:
4783 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2560 2560
Size: 6328
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6328
PsiI TTA'TAA
Cuts at: 3898 3898
Size: 6328
PstI C_TGCA'G
Cuts at: 830 2986 3680 830
Size: 2156 694 3478
Fragments arranged by size:
3478 2156 694
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6328
PvuII CAG'CTG
Cuts at: 3017 3017
Size: 6328
SacI G_AGCT'C
Cuts at: 3572 3572
Size: 6328
SalI G'TCGA_C
Cuts at: 1936 3670 1936
Size: 1734 4594
SanDI GG'GwC_CC
Cuts at: 2560 2560
Size: 6328
SbfI CC_TGCA'GG
Cuts at: 3680 3680
Size: 6328
ScaI AGT'ACT
Cuts at: 4576 6288 4576
Size: 1712 4616
SfcI C'TryA_G
Cuts at: 826 1045 2982 3676 4811 5492 5683 826
Size: 219 1937 694 1135 681 191 1471
Fragments arranged by size:
1937 1471 1135 694 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6328
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6328
SmaI CCC'GGG
Cuts at: 1872 3655 1872
Size: 1783 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2483 3067 4432 5333 5595 820
Size: 197 926 540 584 1365 901 262 1553
Fragments arranged by size:
1553 1365 926 901 584 540 262 197
SnaBI TAC'GTA
Cuts at: 493 4119 493
Size: 3626 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6328
SphI G_CATG'C
Cuts at: 1132 3378 3686 1132
Size: 2246 308 3774
Fragments arranged by size:
3774 2246 308
SspI AAT'ATT
Cuts at: 5 52 2776 3508 4252 5
Size: 47 2724 732 744 2081
Fragments arranged by size:
2724 2081 744 732 47
StuI AGG'CCT
Cuts at: 1517 3387 1517
Size: 1870 4458
StyI C'CwwG_G
Cuts at: 513 1065 2004 3577 3767 513
Size: 552 939 1573 190 3074
Fragments arranged by size:
3074 1573 939 552 190
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2634 2906 3437 4355 4372 4525 4710
Size: 1255 1054 272 531 918 17 153 185
Cuts at: 4710 5155 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1054 918 531 445 272 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2155 3472 4574
Size: 274 80 33 51 1443 178 1317 1102
Cuts at: 4574 6286 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1317 1102 274 178 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2303 2335 6288 6320 2303
Size: 32 3953 32 2311
Fragments arranged by size:
3953 2311 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3273 1316
Size: 1957 4371
XbaI T'CTAG_A
Cuts at: 3664 3664
Size: 6328
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6328
XmaI C'CCGG_G
Cuts at: 1870 3653 1870
Size: 1783 4545
XmnI GAAnn'nnTTC
Cuts at: 2224 2248 4457 2224
Size: 24 2209 4095
Fragments arranged by size:
4095 2209 24
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4136 276
Size: 53 83 186 936 2602 2468
Fragments arranged by size:
2602 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI
PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI
SrfI SwaI XcmI