Restriction map for
pFN21AB7183
(Circular) (Six-base) MAPSORT of: pFN21AB7183.seq Check: 1413 from: 1 to: 6214
pFN21AB7183 6214 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2202 2202
Size: 6214
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2568 4024 278
Size: 53 83 186 936 1032 1456 2468
Fragments arranged by size:
2468 1456 1032 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3456 3557 1937
Size: 1519 101 4594
Fragments arranged by size:
4594 1519 101
Acc65I G'GTAC_C
Cuts at: 3535 3535
Size: 6214
AclI AA'CG_TT
Cuts at: 1563 3513 4341 4714 1563
Size: 1950 828 373 3063
Fragments arranged by size:
3063 1950 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2540 3164 3428 4277 5661 5929 1632
Size: 908 624 264 849 1384 268 1917
Fragments arranged by size:
1917 1384 908 849 624 268 264
AfeI AGC'GCT
Cuts at: 3284 3284
Size: 6214
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6017
AflIII A'CryG_T
Cuts at: 1203 5113 6090 1203
Size: 3910 977 1327
Fragments arranged by size:
3910 1327 977
AgeI A'CCGG_T
Cuts at: 5045 5045
Size: 6214
AhdI GACnn_n'nnGTC
Cuts at: 4943 4943
Size: 6214
AleI CACnn'nnGTG
Cuts at: 1166 2382 1166
Size: 1216 4998
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2047 1184
Size: 863 5351
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2678 2710 2678
Size: 32 6182
AlwNI CAG_nnn'CTG
Cuts at: 2227 2518 2836 3022 3957 5529 2227
Size: 291 318 186 935 1572 2912
Fragments arranged by size:
2912 1572 935 318 291 186
ApaI G_GGCC'C
Cuts at: 2098 2235 2098
Size: 137 6077
ApaLI G'TGCA_C
Cuts at: 4271 5427 4271
Size: 1156 5058
ApoI r'AATT_y
Cuts at: 1493 3523 3749 3901 6010 1493
Size: 2030 226 152 2109 1697
Fragments arranged by size:
2109 2030 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4768 160
Size: 872 3736 1606
Fragments arranged by size:
3736 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6214
AvaI C'yCGr_G
Cuts at: 1870 1943 2091 2544 3256 3356 3539 1870
Size: 73 148 453 712 100 183 4545
Fragments arranged by size:
4545 712 453 183 148 100 73
AvrII C'CTAG_G
Cuts at: 2099 2099
Size: 6214
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2797 2830 2797
Size: 33 6181
BamHI G'GATC_C
Cuts at: 3544 3911 3544
Size: 367 5847
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3363 3535 4990 618
Size: 325 217 627 1576 172 1455 1842
Fragments arranged by size:
1842 1576 1455 627 325 217 172
BanII G_rGCy'C
Cuts at: 1391 2098 2235 2349 2883 3167 3305 3533 1391
Size: 707 137 114 534 284 138 228 4072
Fragments arranged by size:
4072 707 534 284 228 138 137 114
BbeI G_GCGC'C
Cuts at: 3367 3367
Size: 6214
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5243
BbvCI CC'TCA_GC
Cuts at: 2153 2153
Size: 6214
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4405 4439 1903
Size: 34 2468 34 3678
Fragments arranged by size:
3678 2468 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2825 4106 5316 2825
Size: 1281 1210 3723
Fragments arranged by size:
3723 1281 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6214
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2093 2413 2640
Size: 107 122 71 631 605 421 320 227
Cuts at: 2640 2918 4825 136
Size: 278 1907 1525
Fragments arranged by size:
1907 1525 631 605 421 320 278 227
122 107 71
BglII A'GATC_T
Cuts at: 2166 6209 2166
Size: 4043 2171
BlpI GC'TnA_GC
Cuts at: 3631 3631
Size: 6214
Bme1580I G_kGCm'C
Cuts at: 1790 2068 2074 2081 2098 2235 2379 3330 4275
Size: 278 6 7 17 137 144 951 945
Cuts at: 4275 5431 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 951 945 278 144 137 17
7 6
BmgBI CAC'GTC
Cuts at: 1318 3010 3483 1318
Size: 1692 473 4049
Fragments arranged by size:
4049 1692 473
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2084 2646 3945
Size: 416 53 83 399 24 667 562 1299
Cuts at: 3945 4903 442
Size: 958 1753
Fragments arranged by size:
1753 1299 958 667 562 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6214
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2754 2754
Size: 6214
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2191 2222 2363 2705 4874 1239
Size: 952 31 141 342 2169 2579
Fragments arranged by size:
2579 2169 952 342 141 31
Bpu10I CC'TnA_GC
Cuts at: 2153 3264 2153
Size: 1111 5103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3095 3420 4339 5204 5502 3095
Size: 325 919 865 298 3807
Fragments arranged by size:
3807 919 865 325 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3110 4877 882
Size: 617 1611 1767 2219
Fragments arranged by size:
2219 1767 1611 617
BsaAI yAC'GTr
Cuts at: 493 1204 3325 3403 4005 493
Size: 711 2121 78 602 2702
Fragments arranged by size:
2702 2121 711 602 78
BsaBI GATnn'nnATC
Cuts at: 6208 6208
Size: 6214
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2565 3364 4021 4403
Size: 53 83 186 936 1032 799 657 382
Cuts at: 4403 5038 275
Size: 635 1451
Fragments arranged by size:
1451 1032 936 799 657 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4647 5045 5319 5466 5877 4647
Size: 398 274 147 411 4984
Fragments arranged by size:
4984 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2678 2708 2870 2900 1178
Size: 30 165 30 1275 30 162 30 4492
Fragments arranged by size:
4492 1275 165 162 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3482 1186
Size: 2296 3918
BseYI C'CCAG_C
Cuts at: 2130 2227 2573 2629 3196 3200 3232 3279 5417
Size: 97 346 56 567 4 32 47 2138
Cuts at: 5417 5913 2130
Size: 496 2431
Fragments arranged by size:
2431 2138 567 496 346 97 56 47
32 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2180 2486 3032 3380 1163
Size: 573 444 306 546 348 3997
Fragments arranged by size:
3997 573 546 444 348 306
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3664 4425 5453 1540
Size: 275 186 1663 761 1028 2301
Fragments arranged by size:
2301 1663 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2193 2772 2883 3167 4275 4360 5431 2193
Size: 579 111 284 1108 85 1071 2976
Fragments arranged by size:
2976 1108 1071 579 284 111 85
BsmI GAATG_Cn'
Cuts at: 3725 3818 3725
Size: 93 6121
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2068 2074 2081 2098 2193 2235 2349
Size: 399 278 6 7 17 95 42 114
Cuts at: 2349 2379 2772 2883 3167 3305 3330 3533 4275
Size: 30 393 111 284 138 25 203 742
Cuts at: 4275 4360 5431 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 393 284 278 203
138 114 111 95 85 42 30 25
17 7 6
BspEI T'CCGG_A
Cuts at: 5877 5877
Size: 6214
BspHI T'CATG_A
Cuts at: 4103 5833 4103
Size: 1730 4484
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2202 3569 844
Size: 1358 1367 3489
Fragments arranged by size:
3489 1367 1358
BsrBI CCG'CTC
Cuts at: 1394 1823 2090 2626 3268 4101 1394
Size: 429 267 536 642 833 3507
Fragments arranged by size:
3507 833 642 536 429 267
BsrDI GCAATG_nn'
Cuts at: 65 1248 3247 4709 4883 65
Size: 1183 1999 1462 174 1396
Fragments arranged by size:
1999 1462 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4858 5045 1700
Size: 110 3048 187 2869
Fragments arranged by size:
3048 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6214
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6214
BssSI C'ACGA_G
Cuts at: 4274 5286 4274
Size: 1012 5202
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3022 3403 794
Size: 2228 381 3605
Fragments arranged by size:
3605 2228 381
BstBI TT'CG_AA
Cuts at: 5018 5018
Size: 6214
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6214
BstXI CCAn_nnnn'nTGG
Cuts at: 2929 2929
Size: 6214
BstYI r'GATC_y
Cuts at: 1972 2166 3018 3544 3911 4296 4313 5765 5851
Size: 194 852 526 367 385 17 1452 86
Cuts at: 5851 5863 6209 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 852 526 385 367 346 194
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4723
Fragments arranged by size:
4723 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5475
Fragments arranged by size:
5475 683 56
BtsI GCAGTG_nn'
Cuts at: 2499 2937 3730 4524 4544 2499
Size: 438 793 794 20 4169
Fragments arranged by size:
4169 794 793 438 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6214
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6179
DraI TTT'AAA
Cuts at: 3518 3518
Size: 6214
DraIII CAC_nnn'GTG
Cuts at: 3325 3403 3325
Size: 78 6136
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5221 809
Size: 507 3905 1802
Fragments arranged by size:
3905 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2496 2641 3039
Size: 54 1425 50 275 18 666 145 398
Cuts at: 3039 3661 4550 6179 8
Size: 622 889 1629 43
Fragments arranged by size:
1629 1425 889 666 622 398 275 145
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3661 1537
Size: 275 1849 4090
Fragments arranged by size:
4090 1849 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4144 1353
Size: 2791 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2891 2909 4801 5175 5321 387
Size: 1064 138 1302 18 1892 374 146 1280
Fragments arranged by size:
1892 1302 1280 1064 374 146 138 18
EcoICRI GAG'CTC
Cuts at: 2881 3165 2881
Size: 284 5930
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2191 2222 2363 2540 2705 3164 3428
Size: 393 559 31 141 177 165 459 264
Cuts at: 3428 4277 4874 5661 5929 1239
Size: 849 597 787 268 1524
Fragments arranged by size:
1524 849 787 597 559 459 393 268
264 177 165 141 31
EcoNI CCTnn'n_nnAGG
Cuts at: 2888 3026 3371 2888
Size: 138 345 5731
Fragments arranged by size:
5731 345 138
EcoO109I rG'GnC_Cy
Cuts at: 2095 2231 2515 2095
Size: 136 284 5794
Fragments arranged by size:
5794 284 136
EcoRI G'AATT_C
Cuts at: 3523 3901 3523
Size: 378 5836
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2788 3136 2788
Size: 348 5866
FspI TGC'GCA
Cuts at: 4720 4720
Size: 6214
HaeII r_GCGC'y
Cuts at: 1678 2045 2419 3286 3367 5361 1678
Size: 367 374 867 81 1994 2531
Fragments arranged by size:
2531 1994 867 374 367 81
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2435 2467 3418 3450 4855
Size: 32 135 32 863 32 951 32 1405
Cuts at: 4855 4887 4929 4961 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1405 951 863 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3558 3804 669
Size: 988 281 1620 246 3079
Fragments arranged by size:
3079 1620 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6214
HpaI GTT'AAC
Cuts at: 3804 3804
Size: 6214
KasI G'GCGC_C
Cuts at: 3363 3363
Size: 6214
KpnI G_GTAC'C
Cuts at: 3539 3539
Size: 6214
MfeI C'AATT_G
Cuts at: 3813 3813
Size: 6214
MluI A'CGCG_T
Cuts at: 6090 6090
Size: 6214
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5328 5512 1305
Size: 4023 184 2007
Fragments arranged by size:
4023 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2498 2643 10
Size: 54 1425 343 666 145 3581
Fragments arranged by size:
3581 1425 666 343 145 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2382 2720 3449 4172 4531 4690 518
Size: 648 1216 338 729 723 359 159 2042
Fragments arranged by size:
2042 1216 729 723 648 359 338 159
MspA1I CmG'CkG
Cuts at: 1606 3200 3630 4307 5455 5700 1606
Size: 1594 430 677 1148 245 2120
Fragments arranged by size:
2120 1594 1148 677 430 245
NarI GG'CG_CC
Cuts at: 3364 3364
Size: 6214
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4723
Fragments arranged by size:
4723 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6214
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6214
NotI GC'GGCC_GC
Cuts at: 3661 3661
Size: 6214
NspI r_CATG'y
Cuts at: 1132 1229 3346 3572 5117 1132
Size: 97 2117 226 1545 2229
Fragments arranged by size:
2229 2117 1545 226 97
PasI CC'CwG_GG
Cuts at: 1348 2349 1348
Size: 1001 5213
PciI A'CATG_T
Cuts at: 5113 5113
Size: 6214
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2227 2683 2760 2836 1108
Size: 1119 456 77 76 4486
Fragments arranged by size:
4486 1119 456 77 76
PfoI T'CCnGG_A
Cuts at: 2220 2340 2220
Size: 120 6094
PmeI GTTT'AAAC
Cuts at: 3518 3518
Size: 6214
PmlI CAC'GTG
Cuts at: 3325 3403 3325
Size: 78 6136
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2678 2710 4283 4315 5796 5828 2678
Size: 32 1573 32 1481 32 3064
Fragments arranged by size:
3064 1573 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6214
PsiI TTA'TAA
Cuts at: 3784 3784
Size: 6214
PspOMI G'GGCC_C
Cuts at: 2094 2231 2094
Size: 137 6077
PstI C_TGCA'G
Cuts at: 830 2166 2333 2538 2944 3566 830
Size: 1336 167 205 406 622 3478
Fragments arranged by size:
3478 1336 622 406 205 167
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6214
PvuII CAG'CTG
Cuts at: 3200 3200
Size: 6214
SacI G_AGCT'C
Cuts at: 2883 3167 2883
Size: 284 5930
SalI G'TCGA_C
Cuts at: 1936 3556 1936
Size: 1620 4594
SbfI CC_TGCA'GG
Cuts at: 2333 3566 2333
Size: 1233 4981
ScaI AGT'ACT
Cuts at: 4462 6174 4462
Size: 1712 4502
SfcI C'TryA_G
Cuts at: 826 1045 2162 2329 2359 2534 2940 3457 3562
Size: 219 1117 167 30 175 406 517 105
Cuts at: 3562 4697 5378 5569 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1117 681 517 406 219 191
175 167 105 30
SfiI GGCCn_nnn'nGGCC
Cuts at: 2640 2640
Size: 6214
SfoI GGC'GCC
Cuts at: 3365 3365
Size: 6214
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6214
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6214
SmaI CCC'GGG
Cuts at: 1872 2546 3541 1872
Size: 674 995 4545
Fragments arranged by size:
4545 995 674
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3110 3435 4318 5219 5481 820
Size: 197 926 1167 325 883 901 262 1553
Fragments arranged by size:
1553 1167 926 901 883 325 262 197
SnaBI TAC'GTA
Cuts at: 493 4005 493
Size: 3512 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6214
SphI G_CATG'C
Cuts at: 1132 3346 3572 1132
Size: 2214 226 3774
Fragments arranged by size:
3774 2214 226
SspI AAT'ATT
Cuts at: 5 52 4138 5
Size: 47 4086 2081
Fragments arranged by size:
4086 2081 47
StuI AGG'CCT
Cuts at: 1517 2780 3262 3475 1517
Size: 1263 482 213 4256
Fragments arranged by size:
4256 1263 482 213
StyI C'CwwG_G
Cuts at: 513 1065 2004 2099 2457 2754 3653 513
Size: 552 939 95 358 297 899 3074
Fragments arranged by size:
3074 939 899 552 358 297 95
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2341 4241 4258 4411 4596 5041 325
Size: 1255 761 1900 17 153 185 445 1498
Fragments arranged by size:
1900 1498 1255 761 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4460 6172 96
Size: 274 80 33 51 1443 2483 1712 138
Fragments arranged by size:
2483 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6174 6206 6174
Size: 32 6182
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6214
XbaI T'CTAG_A
Cuts at: 3550 3550
Size: 6214
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2145 3197 3329 2145
Size: 1052 132 5030
Fragments arranged by size:
5030 1052 132
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6214
XmaI C'CCGG_G
Cuts at: 1870 2544 3539 1870
Size: 674 995 4545
Fragments arranged by size:
4545 995 674
XmnI GAAnn'nnTTC
Cuts at: 3413 4343 3413
Size: 930 5284
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2566 4022 276
Size: 53 83 186 936 1032 1456 2468
Fragments arranged by size:
2468 1456 1032 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI
FspAI NaeI NgoMIV NruI NsiI PacI PpuMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SrfI SwaI