Restriction map for
pFN21AB7179
(Circular) (Six-base) MAPSORT of: pFN21AB7179.seq Check: 9605 from: 1 to: 6142
pFN21AB7179 6142 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3952 278
Size: 53 83 186 936 2416 2468
Fragments arranged by size:
2468 2416 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3485 1937
Size: 1548 4594
Acc65I G'GTAC_C
Cuts at: 3463 3463
Size: 6142
AclI AA'CG_TT
Cuts at: 1563 4269 4642 1563
Size: 2706 373 3063
Fragments arranged by size:
3063 2706 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2370 2723 4205 5589 5857 1632
Size: 738 353 1482 1384 268 1917
Fragments arranged by size:
1917 1482 1384 738 353 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5945
AflIII A'CryG_T
Cuts at: 1203 3036 5041 6018 1203
Size: 1833 2005 977 1327
Fragments arranged by size:
2005 1833 1327 977
AgeI A'CCGG_T
Cuts at: 4973 4973
Size: 6142
AhdI GACnn_n'nnGTC
Cuts at: 4871 4871
Size: 6142
AleI CACnn'nnGTG
Cuts at: 1166 2405 2979 1166
Size: 1239 574 4329
Fragments arranged by size:
4329 1239 574
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6142
AlwNI CAG_nnn'CTG
Cuts at: 2393 3885 5457 2393
Size: 1492 1572 3078
Fragments arranged by size:
3078 1572 1492
ApaLI G'TGCA_C
Cuts at: 3258 4199 5355 3258
Size: 941 1156 4045
Fragments arranged by size:
4045 1156 941
ApoI r'AATT_y
Cuts at: 1493 2229 3451 3677 3829 5938 1493
Size: 736 1222 226 152 2109 1697
Fragments arranged by size:
2109 1697 1222 736 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4696 160
Size: 872 3664 1606
Fragments arranged by size:
3664 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6142
AvaI C'yCGr_G
Cuts at: 1870 1943 3467 1870
Size: 73 1524 4545
Fragments arranged by size:
4545 1524 73
BamHI G'GATC_C
Cuts at: 2250 2322 3472 3839 2250
Size: 72 1150 367 4553
Fragments arranged by size:
4553 1150 367 72
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3001 3463 4918 618
Size: 325 217 627 1214 462 1455 1842
Fragments arranged by size:
1842 1455 1214 627 462 325 217
BanII G_rGCy'C
Cuts at: 1391 3461 1391
Size: 2070 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5171
BbvCI CC'TCA_GC
Cuts at: 3080 3080
Size: 6142
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4333 4367 1903
Size: 34 2396 34 3678
Fragments arranged by size:
3678 2396 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4034 5244 4034
Size: 1210 4932
BclI T'GATC_A
Cuts at: 1548 2430 2931 3423 1548
Size: 882 501 492 4267
Fragments arranged by size:
4267 882 501 492
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2924 4753 136
Size: 107 122 71 631 605 1252 1829 1525
Fragments arranged by size:
1829 1525 1252 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2581 6137 2581
Size: 3556 2586
BlpI GC'TnA_GC
Cuts at: 3559 3559
Size: 6142
Bme1580I G_kGCm'C
Cuts at: 1790 2895 3262 4203 5359 1790
Size: 1105 367 941 1156 2573
Fragments arranged by size:
2573 1156 1105 941 367
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6142
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2322 3019 3873
Size: 416 53 83 399 24 905 697 854
Cuts at: 3873 4831 442
Size: 958 1753
Fragments arranged by size:
1753 958 905 854 697 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6142
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4802 1239
Size: 3563 2579
Bpu10I CC'TnA_GC
Cuts at: 3080 3080
Size: 6142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4267 5132 5430 4267
Size: 865 298 4979
Fragments arranged by size:
4979 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2815 4805 882
Size: 617 1316 1990 2219
Fragments arranged by size:
2219 1990 1316 617
BsaAI yAC'GTr
Cuts at: 493 1204 2283 3933 493
Size: 711 1079 1650 2702
Fragments arranged by size:
2702 1650 1079 711
BsaBI GATnn'nnATC
Cuts at: 2274 2969 6136 2274
Size: 695 3167 2280
Fragments arranged by size:
3167 2280 695
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3949 4331 4966 275
Size: 53 83 186 936 2416 382 635 1451
Fragments arranged by size:
2416 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2798 4575 4973 5247 5394 5805 2798
Size: 1777 398 274 147 411 3135
Fragments arranged by size:
3135 1777 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2793 2823 1178
Size: 30 165 30 1390 30 4497
Fragments arranged by size:
4497 1390 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2722 2726 2760 1186
Size: 1536 4 34 4568
Fragments arranged by size:
4568 1536 34 4
BseYI C'CCAG_C
Cuts at: 2788 5345 5841 2788
Size: 2557 496 3089
Fragments arranged by size:
3089 2557 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5569
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2999 3592 4353 5381 1540
Size: 275 186 998 593 761 1028 2301
Fragments arranged by size:
2301 1028 998 761 593 275 186
BsiHKAI G_wGCw'C
Cuts at: 3262 4203 4288 5359 3262
Size: 941 85 1071 4045
Fragments arranged by size:
4045 1071 941 85
BsmI GAATG_Cn'
Cuts at: 3653 3746 3653
Size: 93 6049
BsmBI CGTCTCn'nnnn_
Cuts at: 2069 2069
Size: 6142
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2895 3262 3461 4203 4288 5359 1391
Size: 399 1105 367 199 742 85 1071 2174
Fragments arranged by size:
2174 1105 1071 742 399 367 199 85
BspEI T'CCGG_A
Cuts at: 2798 5805 2798
Size: 3007 3135
BspHI T'CATG_A
Cuts at: 4031 5761 4031
Size: 1730 4412
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3497 844
Size: 2653 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3103 3437 4029 1394
Size: 429 1280 334 592 3507
Fragments arranged by size:
3507 1280 592 429 334
BsrDI GCAATG_nn'
Cuts at: 65 1248 4637 4811 65
Size: 1183 3389 174 1396
Fragments arranged by size:
3389 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2446 2875 2998 4786 4973 1700
Size: 110 636 429 123 1788 187 2869
Fragments arranged by size:
2869 1788 636 429 187 123 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6142
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6142
BssSI C'ACGA_G
Cuts at: 2336 4202 5214 2336
Size: 1866 1012 3264
Fragments arranged by size:
3264 1866 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6142
BstBI TT'CG_AA
Cuts at: 4946 4946
Size: 6142
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6142
BstXI CCAn_nnnn'nTGG
Cuts at: 2833 2981 2833
Size: 148 5994
BstYI r'GATC_y
Cuts at: 1972 2040 2250 2322 2581 3472 3839 4224 4241
Size: 68 210 72 259 891 367 385 17
Cuts at: 4241 5693 5779 5791 6137 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 891 385 367 346 259 210
86 72 68 17 12
Bsu36I CC'TnA_GG
Cuts at: 3237 3237
Size: 6142
BtgI C'CryG_G
Cuts at: 513 1065 2620 513
Size: 552 1555 4035
Fragments arranged by size:
4035 1555 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3280 487
Size: 683 56 2054 3349
Fragments arranged by size:
3349 2054 683 56
BtsI GCAGTG_nn'
Cuts at: 2513 2943 3658 4452 4472 2513
Size: 430 715 794 20 4183
Fragments arranged by size:
4183 794 715 430 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6142
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6107
DraI TTT'AAA
Cuts at: 3446 3446
Size: 6142
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5149 809
Size: 507 3833 1802
Fragments arranged by size:
3833 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2448 2835 2996
Size: 54 1425 50 275 18 618 387 161
Cuts at: 2996 3589 4478 6107 8
Size: 593 889 1629 43
Fragments arranged by size:
1629 1425 889 618 593 387 275 161
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2996 3589 1537
Size: 275 1184 593 4090
Fragments arranged by size:
4090 1184 593 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2145 2699 4072 1353
Size: 792 554 1373 3423
Fragments arranged by size:
3423 1373 792 554
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2243 4729 5103 5249 387
Size: 1064 138 654 2486 374 146 1280
Fragments arranged by size:
2486 1280 1064 654 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2370 2723 4205 4802 5589 5857 1239
Size: 393 738 353 1482 597 787 268 1524
Fragments arranged by size:
1524 1482 787 738 597 393 353 268
EcoO109I rG'GnC_Cy
Cuts at: 2712 2712
Size: 6142
EcoRI G'AATT_C
Cuts at: 3451 3829 3451
Size: 378 5764
FspI TGC'GCA
Cuts at: 4648 4648
Size: 6142
HaeII r_GCGC'y
Cuts at: 1678 5289 1678
Size: 3611 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2366 2398 2767 2793 2799
Size: 32 135 32 794 32 369 26 6
Cuts at: 2799 2811 2825 2843 4783 4815 4857 4889 1373
Size: 12 14 18 1940 32 42 32 2626
Fragments arranged by size:
2626 1940 794 369 135 42 32 32
32 32 32 26 18 14 12 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2731 2952 3486 3732 669
Size: 988 281 793 221 534 246 3079
Fragments arranged by size:
3079 988 793 534 281 246 221
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6142
HpaI GTT'AAC
Cuts at: 3732 3732
Size: 6142
KpnI G_GTAC'C
Cuts at: 3467 3467
Size: 6142
MfeI C'AATT_G
Cuts at: 3741 3741
Size: 6142
MluI A'CGCG_T
Cuts at: 6018 6018
Size: 6142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5256 5440 1305
Size: 3951 184 2007
Fragments arranged by size:
3951 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2837 10
Size: 54 1425 343 1005 3315
Fragments arranged by size:
3315 1425 1005 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2405 2960 2963 2979 3401 4100 4459
Size: 648 1239 555 3 16 422 699 359
Cuts at: 4459 4618 518
Size: 159 2042
Fragments arranged by size:
2042 1239 699 648 555 422 359 159
16 3
MspA1I CmG'CkG
Cuts at: 1606 2577 2633 3558 4235 5383 5628 1606
Size: 971 56 925 677 1148 245 2120
Fragments arranged by size:
2120 1148 971 925 677 245 56
NaeI GCC'GGC
Cuts at: 2877 2877
Size: 6142
NcoI C'CATG_G
Cuts at: 513 1065 2620 513
Size: 552 1555 4035
Fragments arranged by size:
4035 1555 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6142
NgoMIV G'CCGG_C
Cuts at: 2875 2875
Size: 6142
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6142
NotI GC'GGCC_GC
Cuts at: 3589 3589
Size: 6142
NspI r_CATG'y
Cuts at: 1132 1229 2629 3040 3500 5045 1132
Size: 97 1400 411 460 1545 2229
Fragments arranged by size:
2229 1545 1400 460 411 97
PasI CC'CwG_GG
Cuts at: 1348 2815 1348
Size: 1467 4675
PciI A'CATG_T
Cuts at: 3036 5041 3036
Size: 2005 4137
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 5240
PfoI T'CCnGG_A
Cuts at: 2528 2528
Size: 6142
PmeI GTTT'AAAC
Cuts at: 3446 3446
Size: 6142
PmlI CAC'GTG
Cuts at: 2283 2283
Size: 6142
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4211 4243 5724 5756 4211
Size: 32 1481 32 4597
Fragments arranged by size:
4597 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2712 2712
Size: 6142
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6142
PsiI TTA'TAA
Cuts at: 3712 3712
Size: 6142
PstI C_TGCA'G
Cuts at: 830 2074 2187 3494 830
Size: 1244 113 1307 3478
Fragments arranged by size:
3478 1307 1244 113
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6142
SalI G'TCGA_C
Cuts at: 1936 3484 1936
Size: 1548 4594
SapI GCTCTTCn'nnn_
Cuts at: 2145 2145
Size: 6142
SbfI CC_TGCA'GG
Cuts at: 3494 3494
Size: 6142
ScaI AGT'ACT
Cuts at: 4390 6102 4390
Size: 1712 4430
SfcI C'TryA_G
Cuts at: 826 1045 2070 2183 3490 4625 5306 5497 826
Size: 219 1025 113 1307 1135 681 191 1471
Fragments arranged by size:
1471 1307 1135 1025 681 219 191 113
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6142
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6142
SmaI CCC'GGG
Cuts at: 1872 3469 1872
Size: 1597 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4246 5147 5409 820
Size: 197 926 2303 901 262 1553
Fragments arranged by size:
2303 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3933 493
Size: 3440 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6142
SphI G_CATG'C
Cuts at: 1132 2629 3500 1132
Size: 1497 871 3774
Fragments arranged by size:
3774 1497 871
SspI AAT'ATT
Cuts at: 5 52 3286 4066 5
Size: 47 3234 780 2081
Fragments arranged by size:
3234 2081 780 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6142
StyI C'CwwG_G
Cuts at: 513 1065 2568 2620 3333 3581 513
Size: 552 1503 52 713 248 3074
Fragments arranged by size:
3074 1503 713 552 248 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2304 3056 3387 4169 4186 4339 4524
Size: 1255 724 752 331 782 17 153 185
Cuts at: 4524 4969 325
Size: 445 1498
Fragments arranged by size:
1498 1255 782 752 724 445 331 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3264 3291 4388
Size: 274 80 33 51 1443 1287 27 1097
Cuts at: 4388 6100 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1287 1097 274 138 80 51
33 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2314 2346 6102 6134 2314
Size: 32 3756 32 2322
Fragments arranged by size:
3756 2322 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2354 1316
Size: 1038 5104
XbaI T'CTAG_A
Cuts at: 3478 3478
Size: 6142
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6142
XmaI C'CCGG_G
Cuts at: 1870 3467 1870
Size: 1597 4545
XmnI GAAnn'nnTTC
Cuts at: 4271 4271
Size: 6142
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3950 276
Size: 53 83 186 936 2416 2468
Fragments arranged by size:
2468 2416 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI