Restriction map for
pFN21AB7177
(Circular) (Six-base) MAPSORT of: pFN21AB7177.seq Check: 6482 from: 1 to: 6151
pFN21AB7177 6151 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3961 278
Size: 53 83 186 936 2425 2468
Fragments arranged by size:
2468 2425 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3494 1937
Size: 1557 4594
Acc65I G'GTAC_C
Cuts at: 3472 3472
Size: 6151
AclI AA'CG_TT
Cuts at: 1563 4278 4651 1563
Size: 2715 373 3063
Fragments arranged by size:
3063 2715 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4214 5598 5866 1632
Size: 2582 1384 268 1917
Fragments arranged by size:
2582 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5954
AflIII A'CryG_T
Cuts at: 1203 5050 6027 1203
Size: 3847 977 1327
Fragments arranged by size:
3847 1327 977
AgeI A'CCGG_T
Cuts at: 4982 4982
Size: 6151
AhdI GACnn_n'nnGTC
Cuts at: 4880 4880
Size: 6151
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6151
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6151
AlwNI CAG_nnn'CTG
Cuts at: 3894 5466 3894
Size: 1572 4579
ApaI G_GGCC'C
Cuts at: 2021 3351 2021
Size: 1330 4821
ApaLI G'TGCA_C
Cuts at: 4208 5364 4208
Size: 1156 4995
ApoI r'AATT_y
Cuts at: 1493 2687 3460 3686 3838 5947 1493
Size: 1194 773 226 152 2109 1697
Fragments arranged by size:
2109 1697 1194 773 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4705 160
Size: 872 3673 1606
Fragments arranged by size:
3673 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6151
AvaI C'yCGr_G
Cuts at: 1870 1943 2021 2134 2269 3476 1870
Size: 73 78 113 135 1207 4545
Fragments arranged by size:
4545 1207 135 113 78 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2309 2342 2309
Size: 33 6118
BamHI G'GATC_C
Cuts at: 3481 3848 3481
Size: 367 5784
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2138 2224 2338 3472 4927
Size: 325 217 627 351 86 114 1134 1455
Cuts at: 4927 618
Size: 1842
Fragments arranged by size:
1842 1455 1134 627 351 325 217 114
86
BanII G_rGCy'C
Cuts at: 1391 2021 2276 3351 3470 1391
Size: 630 255 1075 119 4072
Fragments arranged by size:
4072 1075 630 255 119
BbeI G_GCGC'C
Cuts at: 2142 2228 2142
Size: 86 6065
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2714 3414 928
Size: 971 815 700 3665
Fragments arranged by size:
3665 971 815 700
BbvCI CC'TCA_GC
Cuts at: 2152 2173 2152
Size: 21 6130
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2296 2330 4342 4376 1903
Size: 34 359 34 2012 34 3678
Fragments arranged by size:
3678 2012 359 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4043 5253 4043
Size: 1210 4941
BclI T'GATC_A
Cuts at: 1548 2827 1548
Size: 1279 4872
BfrBI ATG'CAT
Cuts at: 3440 3440
Size: 6151
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2099 2363 4762
Size: 107 122 71 631 605 427 264 2399
Cuts at: 4762 136
Size: 1525
Fragments arranged by size:
2399 1525 631 605 427 264 122 107
71
BglII A'GATC_T
Cuts at: 6146 6146
Size: 6151
BlpI GC'TnA_GC
Cuts at: 3568 3568
Size: 6151
Bme1580I G_kGCm'C
Cuts at: 1790 2021 2343 3351 4212 5368 1790
Size: 231 322 1008 861 1156 2573
Fragments arranged by size:
2573 1156 1008 861 322 231
BmgBI CAC'GTC
Cuts at: 1318 3226 1318
Size: 1908 4243
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2317 3345 3882
Size: 416 53 83 399 24 900 1028 537
Cuts at: 3882 4840 442
Size: 958 1753
Fragments arranged by size:
1753 1028 958 900 537 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4811 1239
Size: 3572 2579
Bpu10I CC'TnA_GC
Cuts at: 2152 2173 2152
Size: 21 6130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2940 4276 5141 5439 2940
Size: 1336 865 298 3652
Fragments arranged by size:
3652 1336 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2032 3332 4814 882
Size: 617 533 1300 1482 2219
Fragments arranged by size:
2219 1482 1300 617 533
BsaAI yAC'GTr
Cuts at: 493 1204 3942 493
Size: 711 2738 2702
Fragments arranged by size:
2738 2702 711
BsaBI GATnn'nnATC
Cuts at: 3439 6145 3439
Size: 2706 3445
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2139 2225 3280 3958
Size: 53 83 186 936 606 86 1055 678
Cuts at: 3958 4340 4975 275
Size: 382 635 1451
Fragments arranged by size:
1451 1055 936 678 635 606 382 186
86 83 53
BsaWI w'CCGG_w
Cuts at: 4584 4982 5256 5403 5814 4584
Size: 398 274 147 411 4921
Fragments arranged by size:
4921 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5926
Fragments arranged by size:
5926 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2140 2303 2783 1186
Size: 954 163 480 4554
Fragments arranged by size:
4554 954 480 163
BseYI C'CCAG_C
Cuts at: 5354 5850 5354
Size: 496 5655
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5578
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2061 2103 2163 3601 4362 5390
Size: 275 186 60 42 60 1438 761 1028
Cuts at: 5390 1540
Size: 2301
Fragments arranged by size:
2301 1438 1028 761 275 186 60 60
42
BsiHKAI G_wGCw'C
Cuts at: 2295 3079 4212 4297 5368 2295
Size: 784 1133 85 1071 3078
Fragments arranged by size:
3078 1133 1071 784 85
BsmI GAATG_Cn'
Cuts at: 3662 3755 3662
Size: 93 6058
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2276 2295 2343 3079 3351 3470
Size: 399 231 255 19 48 736 272 119
Cuts at: 3470 4212 4297 5368 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 736 399 272 255 231
119 85 48 19
BspEI T'CCGG_A
Cuts at: 5814 5814
Size: 6151
BspHI T'CATG_A
Cuts at: 4040 5770 4040
Size: 1730 4421
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3506 844
Size: 2662 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2764 4038 1394
Size: 429 941 1274 3507
Fragments arranged by size:
3507 1274 941 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2451 2457 4646 4820 65
Size: 1183 1203 6 2189 174 1396
Fragments arranged by size:
2189 1396 1203 1183 174 6
BsrFI r'CCGG_y
Cuts at: 1700 1810 4795 4982 1700
Size: 110 2985 187 2869
Fragments arranged by size:
2985 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6151
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6151
BssSI C'ACGA_G
Cuts at: 4211 5223 4211
Size: 1012 5139
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6151
BstBI TT'CG_AA
Cuts at: 4955 4955
Size: 6151
BstEII G'GTnAC_C
Cuts at: 1184 2409 1184
Size: 1225 4926
BstXI CCAn_nnnn'nTGG
Cuts at: 2577 2577
Size: 6151
BstYI r'GATC_y
Cuts at: 1972 3048 3481 3848 4233 4250 5702 5788 5800
Size: 1076 433 367 385 17 1452 86 12
Cuts at: 5800 6146 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1076 433 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2877 2877
Size: 6151
BtgI C'CryG_G
Cuts at: 513 1065 2004 3000 513
Size: 552 939 996 3664
Fragments arranged by size:
3664 996 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5412
Fragments arranged by size:
5412 683 56
BtsI GCAGTG_nn'
Cuts at: 2124 2988 3667 4461 4481 2124
Size: 864 679 794 20 3794
Fragments arranged by size:
3794 864 794 679 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6151
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6116
DraI TTT'AAA
Cuts at: 3455 3455
Size: 6151
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5158 809
Size: 507 3842 1802
Fragments arranged by size:
3842 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2058 2100 2160
Size: 54 1425 50 275 18 228 42 60
Cuts at: 2160 2197 3199 3598 4487 6116 8
Size: 37 1002 399 889 1629 43
Fragments arranged by size:
1629 1425 1002 889 399 275 228 60
54 50 43 42 37 18
EagI C'GGCC_G
Cuts at: 1537 1812 2058 2100 2160 3598 1537
Size: 275 246 42 60 1438 4090
Fragments arranged by size:
4090 1438 275 246 60 42
EarI CTCTTCn'nnn_
Cuts at: 1353 2513 2784 3156 3330 3396 4081 1353
Size: 1160 271 372 174 66 685 3423
Fragments arranged by size:
3423 1160 685 372 271 174 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2056 2062 2077 4738 5112 5258
Size: 1064 138 467 6 15 2661 374 146
Cuts at: 5258 387
Size: 1280
Fragments arranged by size:
2661 1280 1064 467 374 146 138 15
6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4214 4811 5598 5866 1239
Size: 393 2582 597 787 268 1524
Fragments arranged by size:
2582 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2937 2937
Size: 6151
EcoO109I rG'GnC_Cy
Cuts at: 2017 2018 2017
Size: 1 6150
EcoRI G'AATT_C
Cuts at: 3460 3838 3460
Size: 378 5773
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3107 3185 3107
Size: 78 6073
FspI TGC'GCA
Cuts at: 4657 4657
Size: 6151
HaeII r_GCGC'y
Cuts at: 1678 2142 2228 5298 1678
Size: 464 86 3070 2531
Fragments arranged by size:
3070 2531 464 86
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2860 2892 4792 4824 4866
Size: 32 135 32 1288 32 1900 32 42
Cuts at: 4866 4898 1373
Size: 32 2626
Fragments arranged by size:
2626 1900 1288 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3495 3741 669
Size: 988 281 1557 246 3079
Fragments arranged by size:
3079 1557 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6151
HpaI GTT'AAC
Cuts at: 3741 3741
Size: 6151
KasI G'GCGC_C
Cuts at: 2138 2224 2138
Size: 86 6065
KpnI G_GTAC'C
Cuts at: 3476 3476
Size: 6151
MfeI C'AATT_G
Cuts at: 3750 3750
Size: 6151
MluI A'CGCG_T
Cuts at: 6027 6027
Size: 6151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2296 2421 2671 3131 5265 5449 1305
Size: 991 125 250 460 2134 184 2007
Fragments arranged by size:
2134 2007 991 460 250 184 125
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2199 10
Size: 54 1425 343 367 3962
Fragments arranged by size:
3962 1425 367 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3392 4109 4468 4627 518
Size: 648 2226 717 359 159 2042
Fragments arranged by size:
2226 2042 717 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3567 4244 5392 5637 1606
Size: 1961 677 1148 245 2120
Fragments arranged by size:
2120 1961 1148 677 245
NarI GG'CG_CC
Cuts at: 2139 2225 2139
Size: 86 6065
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4660
Fragments arranged by size:
4660 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6151
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6151
NotI GC'GGCC_GC
Cuts at: 2058 3598 2058
Size: 1540 4611
NsiI A_TGCA'T
Cuts at: 3442 3442
Size: 6151
NspI r_CATG'y
Cuts at: 1132 1229 2401 3509 5054 1132
Size: 97 1172 1108 1545 2229
Fragments arranged by size:
2229 1545 1172 1108 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6151
PciI A'CATG_T
Cuts at: 5050 5050
Size: 6151
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2335 1108
Size: 1227 4924
PmeI GTTT'AAAC
Cuts at: 3455 3455
Size: 6151
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4220 4252 5733 5765 4220
Size: 32 1481 32 4606
Fragments arranged by size:
4606 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6151
PsiI TTA'TAA
Cuts at: 3721 3721
Size: 6151
PspOMI G'GGCC_C
Cuts at: 2017 3347 2017
Size: 1330 4821
PstI C_TGCA'G
Cuts at: 830 3503 830
Size: 2673 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6151
SalI G'TCGA_C
Cuts at: 1936 3493 1936
Size: 1557 4594
SapI GCTCTTCn'nnn_
Cuts at: 2513 3156 2513
Size: 643 5508
SbfI CC_TGCA'GG
Cuts at: 3503 3503
Size: 6151
ScaI AGT'ACT
Cuts at: 4399 6111 4399
Size: 1712 4439
SfcI C'TryA_G
Cuts at: 826 1045 2670 3156 3499 4634 5315 5506 826
Size: 219 1625 486 343 1135 681 191 1471
Fragments arranged by size:
1625 1471 1135 681 486 343 219 191
SfoI GGC'GCC
Cuts at: 2140 2226 2140
Size: 86 6065
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6151
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6151
SmaI CCC'GGG
Cuts at: 1872 3478 1872
Size: 1606 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2955 4255 5156 5418 820
Size: 197 926 1012 1300 901 262 1553
Fragments arranged by size:
1553 1300 1012 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3942 493
Size: 3449 2702
SpeI A'CTAG_T
Cuts at: 152 2723 152
Size: 2571 3580
SphI G_CATG'C
Cuts at: 1132 3509 1132
Size: 2377 3774
SspI AAT'ATT
Cuts at: 5 52 3058 4075 5
Size: 47 3006 1017 2081
Fragments arranged by size:
3006 2081 1017 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6151
StyI C'CwwG_G
Cuts at: 513 1065 2004 3590 513
Size: 552 939 1586 3074
Fragments arranged by size:
3074 1586 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2428 4178 4195 4348 4533 4978 325
Size: 1255 848 1750 17 153 185 445 1498
Fragments arranged by size:
1750 1498 1255 848 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4397 6109 96
Size: 274 80 33 51 1443 2420 1712 138
Fragments arranged by size:
2420 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6111 6143 6111
Size: 32 6119
Tth111I GACn'n_nGTC
Cuts at: 1316 2373 1316
Size: 1057 5094
XbaI T'CTAG_A
Cuts at: 3487 3487
Size: 6151
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6151
XmaI C'CCGG_G
Cuts at: 1870 3476 1870
Size: 1606 4545
XmnI GAAnn'nnTTC
Cuts at: 4280 4280
Size: 6151
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3959 276
Size: 53 83 186 936 2425 2468
Fragments arranged by size:
2468 2425 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BplI BsiWI BsmBI
BstZ17I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV
NruI PacI PfoI PmlI PpuMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI
XcmI