Restriction map for
pFN21AB7171
(Circular) (Six-base) MAPSORT of: pFN21AB7171.seq Check: 8875 from: 1 to: 6151
pFN21AB7171 6151 bp
With 182 enzymes: SgfI *
October 20, 2009 10:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3961 278
Size: 53 83 186 936 2425 2468
Fragments arranged by size:
2468 2425 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3494 1937
Size: 1557 4594
Acc65I G'GTAC_C
Cuts at: 3472 3472
Size: 6151
AclI AA'CG_TT
Cuts at: 1563 4278 4651 1563
Size: 2715 373 3063
Fragments arranged by size:
3063 2715 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2174 3361 4214 5598 5866 1632
Size: 542 1187 853 1384 268 1917
Fragments arranged by size:
1917 1384 1187 853 542 268
AfeI AGC'GCT
Cuts at: 2902 2902
Size: 6151
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5954
AflIII A'CryG_T
Cuts at: 1203 2319 3392 5050 6027 1203
Size: 1116 1073 1658 977 1327
Fragments arranged by size:
1658 1327 1116 1073 977
AgeI A'CCGG_T
Cuts at: 4982 4982
Size: 6151
AhdI GACnn_n'nnGTC
Cuts at: 4880 4880
Size: 6151
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6151
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6151
AlwNI CAG_nnn'CTG
Cuts at: 3894 5466 3894
Size: 1572 4579
ApaLI G'TGCA_C
Cuts at: 4208 5364 4208
Size: 1156 4995
ApoI r'AATT_y
Cuts at: 1493 2528 3360 3460 3686 3838 5947 1493
Size: 1035 832 100 226 152 2109 1697
Fragments arranged by size:
2109 1697 1035 832 226 152 100
AseI AT'TA_AT
Cuts at: 160 1032 4705 160
Size: 872 3673 1606
Fragments arranged by size:
3673 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6151
AvaI C'yCGr_G
Cuts at: 1870 1943 3476 1870
Size: 73 1533 4545
Fragments arranged by size:
4545 1533 73
AvrII C'CTAG_G
Cuts at: 2732 2732
Size: 6151
BamHI G'GATC_C
Cuts at: 3481 3848 3481
Size: 367 5784
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3472 4927 618
Size: 325 217 627 1685 1455 1842
Fragments arranged by size:
1842 1685 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2067 3390 3470 1391
Size: 676 1323 80 4072
Fragments arranged by size:
4072 1323 676 80
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2326 2680 3076 928
Size: 971 427 354 396 4003
Fragments arranged by size:
4003 971 427 396 354
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4342 4376 1903
Size: 34 2405 34 3678
Fragments arranged by size:
3678 2405 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4043 5253 4043
Size: 1210 4941
BclI T'GATC_A
Cuts at: 1548 2662 1548
Size: 1114 5037
BfrBI ATG'CAT
Cuts at: 3264 3264
Size: 6151
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4762 136
Size: 107 122 71 631 605 3090 1525
Fragments arranged by size:
3090 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6146 6146
Size: 6151
BlpI GC'TnA_GC
Cuts at: 3568 3568
Size: 6151
Bme1580I G_kGCm'C
Cuts at: 1790 4212 5368 1790
Size: 2422 1156 2573
Fragments arranged by size:
2573 2422 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6151
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2063 2120 2808
Size: 416 53 83 399 24 646 57 688
Cuts at: 2808 3882 4840 442
Size: 1074 958 1753
Fragments arranged by size:
1753 1074 958 688 646 416 399 83
57 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2240 2524 2637 3386 4811 1239
Size: 1001 284 113 749 1425 2579
Fragments arranged by size:
2579 1425 1001 749 284 113
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4276 5141 5439 4276
Size: 865 298 4988
Fragments arranged by size:
4988 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2018 2540 2595 2803 4814 882
Size: 617 519 522 55 208 2011 2219
Fragments arranged by size:
2219 2011 617 522 519 208 55
BsaAI yAC'GTr
Cuts at: 493 1204 3942 493
Size: 711 2738 2702
Fragments arranged by size:
2738 2702 711
BsaBI GATnn'nnATC
Cuts at: 2982 6145 2982
Size: 3163 2988
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 3958 4340 4975
Size: 53 83 186 936 476 1949 382 635
Cuts at: 4975 275
Size: 1451
Fragments arranged by size:
1949 1451 936 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4584 4982 5256 5403 5814 4584
Size: 398 274 147 411 4921
Fragments arranged by size:
4921 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5926
Fragments arranged by size:
5926 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2201 3398 1186
Size: 1015 1197 3939
Fragments arranged by size:
3939 1197 1015
BseYI C'CCAG_C
Cuts at: 5354 5850 5354
Size: 496 5655
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5578
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3601 4362 5390 1540
Size: 275 186 1600 761 1028 2301
Fragments arranged by size:
2301 1600 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3390 4212 4297 5368 3390
Size: 822 85 1071 4173
Fragments arranged by size:
4173 1071 822 85
BsmI GAATG_Cn'
Cuts at: 2466 3096 3662 3755 2466
Size: 630 566 93 4862
Fragments arranged by size:
4862 630 566 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 3390 3470 4212 4297 5368 1391
Size: 399 277 1323 80 742 85 1071 2174
Fragments arranged by size:
2174 1323 1071 742 399 277 85 80
BspEI T'CCGG_A
Cuts at: 5814 5814
Size: 6151
BspHI T'CATG_A
Cuts at: 3057 4040 5770 3057
Size: 983 1730 3438
Fragments arranged by size:
3438 1730 983
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2268 2794 3506 844
Size: 1424 526 712 3489
Fragments arranged by size:
3489 1424 712 526
BsrBI CCG'CTC
Cuts at: 1394 1823 4038 1394
Size: 429 2215 3507
Fragments arranged by size:
3507 2215 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2131 2413 3203 3264 4646 4820 65
Size: 1183 883 282 790 61 1382 174 1396
Fragments arranged by size:
1396 1382 1183 883 790 282 174 61
BsrFI r'CCGG_y
Cuts at: 1700 1810 4795 4982 1700
Size: 110 2985 187 2869
Fragments arranged by size:
2985 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6151
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6151
BssSI C'ACGA_G
Cuts at: 3002 4211 5223 3002
Size: 1209 1012 3930
Fragments arranged by size:
3930 1209 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6151
BstBI TT'CG_AA
Cuts at: 4955 4955
Size: 6151
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6151
BstXI CCAn_nnnn'nTGG
Cuts at: 2356 2851 3191 2356
Size: 495 340 5316
Fragments arranged by size:
5316 495 340
BstYI r'GATC_y
Cuts at: 1972 2572 2638 3481 3848 4233 4250 5702 5788
Size: 600 66 843 367 385 17 1452 86
Cuts at: 5788 5800 6146 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 843 600 385 367 346 86
66 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2054 2082 3141 513
Size: 552 939 50 28 1059 3523
Fragments arranged by size:
3523 1059 939 552 50 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2387 487
Size: 683 56 1161 4251
Fragments arranged by size:
4251 1161 683 56
BtsI GCAGTG_nn'
Cuts at: 3667 4461 4481 3667
Size: 794 20 5337
Fragments arranged by size:
5337 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6151
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6116
DraI TTT'AAA
Cuts at: 2782 2886 3455 2782
Size: 104 569 5478
Fragments arranged by size:
5478 569 104
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5158 809
Size: 507 3842 1802
Fragments arranged by size:
3842 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3138 3598 4487
Size: 54 1425 50 275 18 1308 460 889
Cuts at: 4487 6116 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1308 889 460 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3598 1537
Size: 275 1786 4090
Fragments arranged by size:
4090 1786 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4081 1353
Size: 2728 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2219 4738 5112 5258 387
Size: 1064 138 630 2519 374 146 1280
Fragments arranged by size:
2519 1280 1064 630 374 146 138
EcoICRI GAG'CTC
Cuts at: 3388 3388
Size: 6151
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2174 2240 2524 2637 3361 3386 4214
Size: 393 542 66 284 113 724 25 828
Cuts at: 4214 4811 5598 5866 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 828 787 724 597 542 393 284
268 113 66 25
EcoNI CCTnn'n_nnAGG
Cuts at: 2275 2275
Size: 6151
EcoRI G'AATT_C
Cuts at: 3360 3460 3838 3360
Size: 100 378 5673
Fragments arranged by size:
5673 378 100
FspI TGC'GCA
Cuts at: 4657 4657
Size: 6151
HaeII r_GCGC'y
Cuts at: 1678 2904 5298 1678
Size: 1226 2394 2531
Fragments arranged by size:
2531 2394 1226
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2030 2062 2630 2662 2783
Size: 32 135 32 458 32 568 32 121
Cuts at: 2783 2815 4792 4824 4866 4898 1373
Size: 32 1977 32 42 32 2626
Fragments arranged by size:
2626 1977 568 458 135 121 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2751 3495 3741 669
Size: 988 281 813 744 246 3079
Fragments arranged by size:
3079 988 813 744 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6151
HpaI GTT'AAC
Cuts at: 2751 3741 2751
Size: 990 5161
KpnI G_GTAC'C
Cuts at: 3476 3476
Size: 6151
MfeI C'AATT_G
Cuts at: 3750 3750
Size: 6151
MluI A'CGCG_T
Cuts at: 6027 6027
Size: 6151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2716 5265 5449 1305
Size: 1411 2549 184 2007
Fragments arranged by size:
2549 2007 1411 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4329
Fragments arranged by size:
4329 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2053 2406 2471 4109 4468 4627 518
Size: 648 887 353 65 1638 359 159 2042
Fragments arranged by size:
2042 1638 887 648 359 353 159 65
MspA1I CmG'CkG
Cuts at: 1606 2164 3567 4244 5392 5637 1606
Size: 558 1403 677 1148 245 2120
Fragments arranged by size:
2120 1403 1148 677 558 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2054 2082 513
Size: 552 939 50 28 4582
Fragments arranged by size:
4582 939 552 50 28
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6151
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6151
NotI GC'GGCC_GC
Cuts at: 3598 3598
Size: 6151
NsiI A_TGCA'T
Cuts at: 3266 3266
Size: 6151
NspI r_CATG'y
Cuts at: 1132 1229 2323 3509 5054 1132
Size: 97 1094 1186 1545 2229
Fragments arranged by size:
2229 1545 1186 1094 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6151
PciI A'CATG_T
Cuts at: 2319 5050 2319
Size: 2731 3420
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2126 1108
Size: 1018 5133
PfoI T'CCnGG_A
Cuts at: 2946 2946
Size: 6151
PmeI GTTT'AAAC
Cuts at: 3455 3455
Size: 6151
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4220 4252 5733 5765 4220
Size: 32 1481 32 4606
Fragments arranged by size:
4606 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6151
PsiI TTA'TAA
Cuts at: 3721 3721
Size: 6151
PstI C_TGCA'G
Cuts at: 830 2722 3503 830
Size: 1892 781 3478
Fragments arranged by size:
3478 1892 781
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6151
SacI G_AGCT'C
Cuts at: 3390 3390
Size: 6151
SalI G'TCGA_C
Cuts at: 1936 3493 1936
Size: 1557 4594
SbfI CC_TGCA'GG
Cuts at: 3503 3503
Size: 6151
ScaI AGT'ACT
Cuts at: 4399 6111 4399
Size: 1712 4439
SfcI C'TryA_G
Cuts at: 826 1045 2499 2718 3499 4634 5315 5506 826
Size: 219 1454 219 781 1135 681 191 1471
Fragments arranged by size:
1471 1454 1135 781 681 219 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6151
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6151
SmaI CCC'GGG
Cuts at: 1872 3478 1872
Size: 1606 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4255 5156 5418 820
Size: 197 926 2312 901 262 1553
Fragments arranged by size:
2312 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3942 493
Size: 3449 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6151
SphI G_CATG'C
Cuts at: 1132 3509 1132
Size: 2377 3774
SspI AAT'ATT
Cuts at: 5 52 4075 5
Size: 47 4023 2081
Fragments arranged by size:
4023 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6151
StyI C'CwwG_G
Cuts at: 513 1065 2004 2054 2082 2199 2669 2732 3228
Size: 552 939 50 28 117 470 63 496
Cuts at: 3228 3590 513
Size: 362 3074
Fragments arranged by size:
3074 939 552 496 470 362 117 63
50 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2131 2434 3416 4178 4195 4348 4533
Size: 1255 551 303 982 762 17 153 185
Cuts at: 4533 4978 325
Size: 445 1498
Fragments arranged by size:
1498 1255 982 762 551 445 303 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3440 4397 6109
Size: 274 80 33 51 1443 1463 957 1712
Cuts at: 6109 96
Size: 138
Fragments arranged by size:
1712 1463 1443 957 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2793 2825 2928 2960 3391 3423 6111 6143 2793
Size: 32 103 32 431 32 2688 32 2801
Fragments arranged by size:
2801 2688 431 103 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6151
XbaI T'CTAG_A
Cuts at: 3487 3487
Size: 6151
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2516 2516
Size: 6151
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6151
XmaI C'CCGG_G
Cuts at: 1870 3476 1870
Size: 1606 4545
XmnI GAAnn'nnTTC
Cuts at: 4280 4280
Size: 6151
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3959 276
Size: 53 83 186 936 2425 2468
Fragments arranged by size:
2468 2425 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI