Restriction map for
pFN21AB7161
(Circular) (Six-base) MAPSORT of: pFN21AB7161.seq Check: 8063 from: 1 to: 5824
pFN21AB7161 5824 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3634 278
Size: 53 83 186 936 2098 2468
Fragments arranged by size:
2468 2098 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3167 1937
Size: 1230 4594
Acc65I G'GTAC_C
Cuts at: 3145 3145
Size: 5824
AclI AA'CG_TT
Cuts at: 1563 2037 3951 4324 1563
Size: 474 1914 373 3063
Fragments arranged by size:
3063 1914 474 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3887 5271 5539 1632
Size: 2255 1384 268 1917
Fragments arranged by size:
2255 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5627
AflIII A'CryG_T
Cuts at: 1203 4723 5700 1203
Size: 3520 977 1327
Fragments arranged by size:
3520 1327 977
AgeI A'CCGG_T
Cuts at: 4655 4655
Size: 5824
AhdI GACnn_n'nnGTC
Cuts at: 2404 4553 2404
Size: 2149 3675
AleI CACnn'nnGTG
Cuts at: 1166 2619 1166
Size: 1453 4371
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5824
AlwNI CAG_nnn'CTG
Cuts at: 2392 3567 5139 2392
Size: 1175 1572 3077
Fragments arranged by size:
3077 1572 1175
ApaI G_GGCC'C
Cuts at: 2609 2609
Size: 5824
ApaLI G'TGCA_C
Cuts at: 3881 5037 3881
Size: 1156 4668
ApoI r'AATT_y
Cuts at: 1493 2897 3133 3359 3511 5620 1493
Size: 1404 236 226 152 2109 1697
Fragments arranged by size:
2109 1697 1404 236 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4378 160
Size: 872 3346 1606
Fragments arranged by size:
3346 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
AvaI C'yCGr_G
Cuts at: 1870 1943 2678 3149 1870
Size: 73 735 471 4545
Fragments arranged by size:
4545 735 471 73
AvrII C'CTAG_G
Cuts at: 2813 2813
Size: 5824
BamHI G'GATC_C
Cuts at: 3154 3521 3154
Size: 367 5457
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2215 2378 2869 3145 4600
Size: 325 217 627 428 163 491 276 1455
Cuts at: 4600 618
Size: 1842
Fragments arranged by size:
1842 1455 627 491 428 325 276 217
163
BanII G_rGCy'C
Cuts at: 1391 2309 2336 2560 2609 2823 2920 3143 1391
Size: 918 27 224 49 214 97 223 4072
Fragments arranged by size:
4072 918 224 223 214 97 49 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2079 928
Size: 971 180 4673
Fragments arranged by size:
4673 971 180
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4015 4049 1903
Size: 34 50 34 1994 34 3678
Fragments arranged by size:
3678 1994 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2365 3716 4926 2365
Size: 1351 1210 3263
Fragments arranged by size:
3263 1351 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5824
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4435 136
Size: 107 122 71 631 605 2763 1525
Fragments arranged by size:
2763 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5819 5819
Size: 5824
BlpI GC'TnA_GC
Cuts at: 3241 3241
Size: 5824
Bme1580I G_kGCm'C
Cuts at: 1790 2220 2609 2874 3885 5041 1790
Size: 430 389 265 1011 1156 2573
Fragments arranged by size:
2573 1156 1011 430 389 265
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5824
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2086 2250 3555
Size: 416 53 83 399 24 669 164 1305
Cuts at: 3555 4513 442
Size: 958 1753
Fragments arranged by size:
1753 1305 958 669 416 399 164 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5824
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2137 2236 2137
Size: 99 5725
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2876 4484 1239
Size: 1637 1608 2579
Fragments arranged by size:
2579 1637 1608
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2534 3949 4814 5112 2534
Size: 1415 865 298 3246
Fragments arranged by size:
3246 1415 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4487 882
Size: 617 2988 2219
Fragments arranged by size:
2988 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3615 493
Size: 711 2411 2702
Fragments arranged by size:
2702 2411 711
BsaBI GATnn'nnATC
Cuts at: 2516 5818 2516
Size: 3302 2522
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3631 4013 4648 275
Size: 53 83 186 936 2098 382 635 1451
Fragments arranged by size:
2098 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4257 4655 4929 5076 5487 4257
Size: 398 274 147 411 4594
Fragments arranged by size:
4594 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2033 2063 1178
Size: 30 165 30 630 30 4939
Fragments arranged by size:
4939 630 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2113 2126 2219 2222 2225 2308 2360 2453
Size: 927 13 93 3 3 83 52 93
Cuts at: 2453 2495 2756 2813 2874 1186
Size: 42 261 57 61 4136
Fragments arranged by size:
4136 927 261 93 93 83 61 57
52 42 13 3 3
BseYI C'CCAG_C
Cuts at: 2783 5027 5523 2783
Size: 2244 496 3084
Fragments arranged by size:
3084 2244 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2861 1163
Size: 573 1125 4126
Fragments arranged by size:
4126 1125 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3274 4035 5063 1540
Size: 275 186 1273 761 1028 2301
Fragments arranged by size:
2301 1273 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2309 2336 2796 2823 3885 3970 5041 2309
Size: 27 460 27 1062 85 1071 3092
Fragments arranged by size:
3092 1071 1062 460 85 27 27
BsmI GAATG_Cn'
Cuts at: 2250 2770 3335 3428 2250
Size: 520 565 93 4646
Fragments arranged by size:
4646 565 520 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3115 3115
Size: 5824
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2220 2309 2336 2560 2609 2796 2823
Size: 399 430 89 27 224 49 187 27
Cuts at: 2823 2874 2920 3143 3885 3970 5041 1391
Size: 51 46 223 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 430 399 224 223 187
89 85 51 49 46 27 27
BspEI T'CCGG_A
Cuts at: 5487 5487
Size: 5824
BspHI T'CATG_A
Cuts at: 3713 5443 3713
Size: 1730 4094
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3179 844
Size: 2335 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3711 1394
Size: 429 1888 3507
Fragments arranged by size:
3507 1888 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4319 4493 65
Size: 1183 3071 174 1396
Fragments arranged by size:
3071 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4468 4655 1700
Size: 110 2658 187 2869
Fragments arranged by size:
2869 2658 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5824
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5824
BssSI C'ACGA_G
Cuts at: 3884 4896 3884
Size: 1012 4812
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3062 794
Size: 2268 3556
BstBI TT'CG_AA
Cuts at: 4628 4628
Size: 5824
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5824
BstXI CCAn_nnnn'nTGG
Cuts at: 3073 3073
Size: 5824
BstYI r'GATC_y
Cuts at: 1972 3154 3521 3906 3923 5375 5461 5473 5819
Size: 1182 367 385 17 1452 86 12 346
Cuts at: 5819 1972
Size: 1977
Fragments arranged by size:
1977 1452 1182 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2202 2202
Size: 5824
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5272
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5085
Fragments arranged by size:
5085 683 56
BtsI GCAGTG_nn'
Cuts at: 3340 4134 4154 3340
Size: 794 20 5010
Fragments arranged by size:
5010 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5824
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5789
DraI TTT'AAA
Cuts at: 3128 3128
Size: 5824
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4831 809
Size: 507 3515 1802
Fragments arranged by size:
3515 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2439 3075 3271
Size: 54 1425 50 275 18 609 636 196
Cuts at: 3271 4160 5789 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 636 609 275 196 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3271 1537
Size: 275 1459 4090
Fragments arranged by size:
4090 1459 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2103 2137 2167 2185 3021 3754 1353
Size: 750 34 30 18 836 733 3423
Fragments arranged by size:
3423 836 750 733 34 30 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2116 4411 4785 4931 387
Size: 1064 138 527 2295 374 146 1280
Fragments arranged by size:
2295 1280 1064 527 374 146 138
EcoICRI GAG'CTC
Cuts at: 2307 2334 2821 2307
Size: 27 487 5310
Fragments arranged by size:
5310 487 27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2876 3887 4484 5271 5539 1239
Size: 393 1244 1011 597 787 268 1524
Fragments arranged by size:
1524 1244 1011 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2273 2285 2389 2606 2273
Size: 12 104 217 5491
Fragments arranged by size:
5491 217 104 12
EcoRI G'AATT_C
Cuts at: 2897 3133 3511 2897
Size: 236 378 5210
Fragments arranged by size:
5210 378 236
FspI TGC'GCA
Cuts at: 4330 4330
Size: 5824
HaeII r_GCGC'y
Cuts at: 1678 4971 1678
Size: 3293 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2033 2065 2221 2253 2390
Size: 32 135 32 461 32 156 32 137
Cuts at: 2390 2422 4465 4497 4539 4571 1373
Size: 32 2043 32 42 32 2626
Fragments arranged by size:
2626 2043 461 156 137 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2041 3168 3414 669
Size: 988 281 103 1127 246 3079
Fragments arranged by size:
3079 1127 988 281 246 103
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5824
HpaI GTT'AAC
Cuts at: 3414 3414
Size: 5824
KpnI G_GTAC'C
Cuts at: 3149 3149
Size: 5824
MfeI C'AATT_G
Cuts at: 3045 3423 3045
Size: 378 5446
MluI A'CGCG_T
Cuts at: 5700 5700
Size: 5824
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4938 5122 1305
Size: 3633 184 2007
Fragments arranged by size:
3633 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3077 10
Size: 54 1425 343 1245 2757
Fragments arranged by size:
2757 1425 1245 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2619 3071 3782 4141 4300 518
Size: 648 1453 452 711 359 159 2042
Fragments arranged by size:
2042 1453 711 648 452 359 159
MspA1I CmG'CkG
Cuts at: 1606 3240 3917 5065 5310 1606
Size: 1634 677 1148 245 2120
Fragments arranged by size:
2120 1634 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5272
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5824
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5824
NotI GC'GGCC_GC
Cuts at: 3271 3271
Size: 5824
NspI r_CATG'y
Cuts at: 1132 1229 2677 3182 4727 1132
Size: 97 1448 505 1545 2229
Fragments arranged by size:
2229 1545 1448 505 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5824
PciI A'CATG_T
Cuts at: 4723 4723
Size: 5824
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5824
PmeI GTTT'AAAC
Cuts at: 3128 3128
Size: 5824
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3893 3925 5406 5438 3893
Size: 32 1481 32 4279
Fragments arranged by size:
4279 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2273 2285 2273
Size: 12 5812
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5824
PsiI TTA'TAA
Cuts at: 3394 3394
Size: 5824
PspOMI G'GGCC_C
Cuts at: 2605 2605
Size: 5824
PstI C_TGCA'G
Cuts at: 830 3176 830
Size: 2346 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5824
SacI G_AGCT'C
Cuts at: 2309 2336 2823 2309
Size: 27 487 5310
Fragments arranged by size:
5310 487 27
SalI G'TCGA_C
Cuts at: 1936 3166 1936
Size: 1230 4594
SbfI CC_TGCA'GG
Cuts at: 3176 3176
Size: 5824
ScaI AGT'ACT
Cuts at: 2946 4072 5784 2946
Size: 1126 1712 2986
Fragments arranged by size:
2986 1712 1126
SfcI C'TryA_G
Cuts at: 826 1045 3172 4307 4988 5179 826
Size: 219 2127 1135 681 191 1471
Fragments arranged by size:
2127 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5824
SmaI CCC'GGG
Cuts at: 1872 3151 1872
Size: 1279 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2549 3928 4829 5091 820
Size: 197 926 606 1379 901 262 1553
Fragments arranged by size:
1553 1379 926 901 606 262 197
SnaBI TAC'GTA
Cuts at: 493 3615 493
Size: 3122 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5824
SphI G_CATG'C
Cuts at: 1132 2677 3182 1132
Size: 1545 505 3774
Fragments arranged by size:
3774 1545 505
SspI AAT'ATT
Cuts at: 5 52 3748 5
Size: 47 3696 2081
Fragments arranged by size:
3696 2081 47
StuI AGG'CCT
Cuts at: 1517 2643 1517
Size: 1126 4698
StyI C'CwwG_G
Cuts at: 513 1065 2813 3263 513
Size: 552 1748 450 3074
Fragments arranged by size:
3074 1748 552 450
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2827 3851 3868 4021 4206 4651 325
Size: 1255 1247 1024 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1247 1024 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2142 2523 2944
Size: 274 80 33 51 1443 165 381 421
Cuts at: 2944 4070 5782 96
Size: 1126 1712 138
Fragments arranged by size:
1712 1443 1126 421 381 274 165 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5784 5816 5784
Size: 32 5792
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5824
XbaI T'CTAG_A
Cuts at: 3160 3160
Size: 5824
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5824
XmaI C'CCGG_G
Cuts at: 1870 3149 1870
Size: 1279 4545
XmnI GAAnn'nnTTC
Cuts at: 3953 3953
Size: 5824
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3632 276
Size: 53 83 186 936 2098 2468
Fragments arranged by size:
2468 2098 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I DraIII EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspXI PsrI PvuII RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI