Restriction map for
pFN21AB7159
(Circular) (Six-base) MAPSORT of: pFN21AB7159.seq Check: 8352 from: 1 to: 5800
pFN21AB7159 5800 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2333 2333
Size: 5800
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3610 278
Size: 53 83 186 936 2074 2468
Fragments arranged by size:
2468 2074 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3143 1937
Size: 1206 4594
Acc65I G'GTAC_C
Cuts at: 3121 3121
Size: 5800
AclI AA'CG_TT
Cuts at: 1563 3927 4300 1563
Size: 2364 373 3063
Fragments arranged by size:
3063 2364 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3863 5247 5515 1632
Size: 2231 1384 268 1917
Fragments arranged by size:
2231 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2668 820
Size: 197 1651 3952
Fragments arranged by size:
3952 1651 197
AflIII A'CryG_T
Cuts at: 1203 4699 5676 1203
Size: 3496 977 1327
Fragments arranged by size:
3496 1327 977
AgeI A'CCGG_T
Cuts at: 4631 4631
Size: 5800
AhdI GACnn_n'nnGTC
Cuts at: 4529 4529
Size: 5800
AleI CACnn'nnGTG
Cuts at: 1166 2343 1166
Size: 1177 4623
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5800
AlwNI CAG_nnn'CTG
Cuts at: 2367 3543 5115 2367
Size: 1176 1572 3052
Fragments arranged by size:
3052 1572 1176
ApaLI G'TGCA_C
Cuts at: 2521 3857 5013 2521
Size: 1336 1156 3308
Fragments arranged by size:
3308 1336 1156
ApoI r'AATT_y
Cuts at: 1493 2695 3010 3109 3335 3487 5596 1493
Size: 1202 315 99 226 152 2109 1697
Fragments arranged by size:
2109 1697 1202 315 226 152 99
AseI AT'TA_AT
Cuts at: 160 1032 4354 160
Size: 872 3322 1606
Fragments arranged by size:
3322 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
AvaI C'yCGr_G
Cuts at: 1870 1943 3125 1870
Size: 73 1182 4545
Fragments arranged by size:
4545 1182 73
BamHI G'GATC_C
Cuts at: 2796 3130 3497 2796
Size: 334 367 5099
Fragments arranged by size:
5099 367 334
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2147 3121 4576 618
Size: 325 217 627 360 974 1455 1842
Fragments arranged by size:
1842 1455 974 627 360 325 217
BanII G_rGCy'C
Cuts at: 1391 3119 1391
Size: 1728 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3057 928
Size: 971 1158 3671
Fragments arranged by size:
3671 1158 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3991 4025 1903
Size: 34 2054 34 3678
Fragments arranged by size:
3678 2054 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3692 4902 3692
Size: 1210 4590
BclI T'GATC_A
Cuts at: 1548 2133 3018 1548
Size: 585 885 4330
Fragments arranged by size:
4330 885 585
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2039 2417 4411
Size: 107 122 71 631 605 367 378 1994
Cuts at: 4411 136
Size: 1525
Fragments arranged by size:
1994 1525 631 605 378 367 122 107
71
BglII A'GATC_T
Cuts at: 2844 5795 2844
Size: 2951 2849
BlpI GC'TnA_GC
Cuts at: 3217 3217
Size: 5800
Bme1580I G_kGCm'C
Cuts at: 1790 2525 3861 5017 1790
Size: 735 1336 1156 2573
Fragments arranged by size:
2573 1336 1156 735
BmgBI CAC'GTC
Cuts at: 1318 2994 1318
Size: 1676 4124
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2377 3531 4489
Size: 416 53 83 399 24 960 1154 958
Cuts at: 4489 442
Size: 1753
Fragments arranged by size:
1753 1154 960 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5800
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2503 4460 1239
Size: 1264 1957 2579
Fragments arranged by size:
2579 1957 1264
Bpu10I CC'TnA_GC
Cuts at: 2237 2501 2237
Size: 264 5536
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3925 4790 5088 3925
Size: 865 298 4637
Fragments arranged by size:
4637 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4463 882
Size: 617 2964 2219
Fragments arranged by size:
2964 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3591 493
Size: 711 2387 2702
Fragments arranged by size:
2702 2387 711
BsaBI GATnn'nnATC
Cuts at: 2820 5794 2820
Size: 2974 2826
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3607 3989 4624 275
Size: 53 83 186 936 2074 382 635 1451
Fragments arranged by size:
2074 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2613 4233 4631 4905 5052 5463 2613
Size: 1620 398 274 147 411 2950
Fragments arranged by size:
2950 1620 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5575
Fragments arranged by size:
5575 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5800
BseYI C'CCAG_C
Cuts at: 2566 5003 5499 2566
Size: 2437 496 2867
Fragments arranged by size:
2867 2437 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2305 1163
Size: 573 569 4658
Fragments arranged by size:
4658 573 569
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3250 4011 5039 1540
Size: 275 186 1249 761 1028 2301
Fragments arranged by size:
2301 1249 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2226 2525 3861 3946 5017 2226
Size: 299 1336 85 1071 3009
Fragments arranged by size:
3009 1336 1071 299 85
BsmI GAATG_Cn'
Cuts at: 2900 3311 3404 2900
Size: 411 93 5296
Fragments arranged by size:
5296 411 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2226 2525 3119 3861 3946 5017 1391
Size: 399 436 299 594 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 594 436 399 299 85
BspEI T'CCGG_A
Cuts at: 5463 5463
Size: 5800
BspHI T'CATG_A
Cuts at: 3689 5419 3689
Size: 1730 4070
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2324 2333 3155 844
Size: 1480 9 822 3489
Fragments arranged by size:
3489 1480 822 9
BsrBI CCG'CTC
Cuts at: 1394 1823 3687 1394
Size: 429 1864 3507
Fragments arranged by size:
3507 1864 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2349 4295 4469 65
Size: 1183 1101 1946 174 1396
Fragments arranged by size:
1946 1396 1183 1101 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4444 4631 1700
Size: 110 2634 187 2869
Fragments arranged by size:
2869 2634 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5800
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5800
BssSI C'ACGA_G
Cuts at: 3860 4872 3860
Size: 1012 4788
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5800
BstBI TT'CG_AA
Cuts at: 4604 4604
Size: 5800
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5800
BstYI r'GATC_y
Cuts at: 1972 2796 2844 3130 3497 3882 3899 5351 5437
Size: 824 48 286 367 385 17 1452 86
Cuts at: 5437 5449 5795 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 824 385 367 346 286 86
48 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5248
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5061
Fragments arranged by size:
5061 683 56
BtsI GCAGTG_nn'
Cuts at: 2226 3316 4110 4130 2226
Size: 1090 794 20 3896
Fragments arranged by size:
3896 1090 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5800
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2752 2787 529
Size: 35 2188 35 3542
Fragments arranged by size:
3542 2188 35 35
DraI TTT'AAA
Cuts at: 3070 3104 3070
Size: 34 5766
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4807 809
Size: 507 3491 1802
Fragments arranged by size:
3491 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2196 2409 2628
Size: 54 1425 50 275 18 366 213 219
Cuts at: 2628 2979 3247 4136 5765 8
Size: 351 268 889 1629 43
Fragments arranged by size:
1629 1425 889 366 351 275 268 219
213 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3247 1537
Size: 275 1435 4090
Fragments arranged by size:
4090 1435 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2396 3730 1353
Size: 1043 1334 3423
Fragments arranged by size:
3423 1334 1043
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4387 4761 4907 387
Size: 1064 138 2798 374 146 1280
Fragments arranged by size:
2798 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2503 3863 4460 5247 5515 1239
Size: 393 871 1360 597 787 268 1524
Fragments arranged by size:
1524 1360 871 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2288 2791 2288
Size: 503 5297
EcoRI G'AATT_C
Cuts at: 2695 3010 3109 3487 2695
Size: 315 99 378 5008
Fragments arranged by size:
5008 378 315 99
FspI TGC'GCA
Cuts at: 4306 4306
Size: 5800
HaeII r_GCGC'y
Cuts at: 1678 4947 1678
Size: 3269 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2932 2964 3010 3042 4441
Size: 32 135 32 1360 32 46 32 1399
Cuts at: 4441 4473 4515 4547 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1399 1360 135 46 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3144 3390 669
Size: 988 281 1206 246 3079
Fragments arranged by size:
3079 1206 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2270 748
Size: 1522 4278
HpaI GTT'AAC
Cuts at: 3390 3390
Size: 5800
KpnI G_GTAC'C
Cuts at: 3125 3125
Size: 5800
MfeI C'AATT_G
Cuts at: 3399 3399
Size: 5800
MluI A'CGCG_T
Cuts at: 5676 5676
Size: 5800
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4914 5098 1305
Size: 3609 184 2007
Fragments arranged by size:
3609 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3978
Fragments arranged by size:
3978 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2081 2255 2343 2465 2538 3758 4117
Size: 648 915 174 88 122 73 1220 359
Cuts at: 4117 4276 518
Size: 159 2042
Fragments arranged by size:
2042 1220 915 648 359 174 159 122
88 73
MspA1I CmG'CkG
Cuts at: 1606 3216 3893 5041 5286 1606
Size: 1610 677 1148 245 2120
Fragments arranged by size:
2120 1610 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5248
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5800
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5800
NotI GC'GGCC_GC
Cuts at: 3247 3247
Size: 5800
NspI r_CATG'y
Cuts at: 1132 1229 3158 4703 1132
Size: 97 1929 1545 2229
Fragments arranged by size:
2229 1929 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5800
PciI A'CATG_T
Cuts at: 4699 4699
Size: 5800
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5800
PmeI GTTT'AAAC
Cuts at: 3104 3104
Size: 5800
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3869 3901 5382 5414 3869
Size: 32 1481 32 4255
Fragments arranged by size:
4255 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5800
PsiI TTA'TAA
Cuts at: 3370 3370
Size: 5800
PstI C_TGCA'G
Cuts at: 830 3152 830
Size: 2322 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5800
SalI G'TCGA_C
Cuts at: 1936 3142 1936
Size: 1206 4594
SbfI CC_TGCA'GG
Cuts at: 3152 3152
Size: 5800
ScaI AGT'ACT
Cuts at: 4048 5760 4048
Size: 1712 4088
SfcI C'TryA_G
Cuts at: 826 1045 3148 4283 4964 5155 826
Size: 219 2103 1135 681 191 1471
Fragments arranged by size:
2103 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2417 2417
Size: 5800
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5800
SmaI CCC'GGG
Cuts at: 1872 3127 1872
Size: 1255 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2668 3904 4805 5067 820
Size: 197 926 725 1236 901 262 1553
Fragments arranged by size:
1553 1236 926 901 725 262 197
SnaBI TAC'GTA
Cuts at: 493 3591 493
Size: 3098 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5800
SphI G_CATG'C
Cuts at: 1132 3158 1132
Size: 2026 3774
SspI AAT'ATT
Cuts at: 5 52 3724 5
Size: 47 3672 2081
Fragments arranged by size:
3672 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5800
StyI C'CwwG_G
Cuts at: 513 1065 3239 513
Size: 552 2174 3074
Fragments arranged by size:
3074 2174 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2558 2841 2885 3827 3844 3997 4182
Size: 1255 978 283 44 942 17 153 185
Cuts at: 4182 4627 325
Size: 445 1498
Fragments arranged by size:
1498 1255 978 942 445 283 185 153
44 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4046 5758 96
Size: 274 80 33 51 1443 2069 1712 138
Fragments arranged by size:
2069 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5760 5792 5760
Size: 32 5768
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5800
XbaI T'CTAG_A
Cuts at: 2818 3136 2818
Size: 318 5482
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5800
XmaI C'CCGG_G
Cuts at: 1870 3125 1870
Size: 1255 4545
XmnI GAAnn'nnTTC
Cuts at: 3929 3929
Size: 5800
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3608 276
Size: 53 83 186 936 2074 2468
Fragments arranged by size:
2468 2074 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfoI SrfI SwaI XcmI