Restriction map for
pFN21AB7157
(Circular) (Six-base) MAPSORT of: pFN21AB7157.seq Check: 2942 from: 1 to: 5785
pFN21AB7157 5785 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2683 2683
Size: 5785
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3595 278
Size: 53 83 186 936 2059 2468
Fragments arranged by size:
2468 2059 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3128 1937
Size: 1191 4594
Acc65I G'GTAC_C
Cuts at: 3106 3106
Size: 5785
AclI AA'CG_TT
Cuts at: 1563 3912 4285 1563
Size: 2349 373 3063
Fragments arranged by size:
3063 2349 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2348 3848 5232 5500 1632
Size: 716 1500 1384 268 1917
Fragments arranged by size:
1917 1500 1384 716 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5588
AflIII A'CryG_T
Cuts at: 1203 4684 5661 1203
Size: 3481 977 1327
Fragments arranged by size:
3481 1327 977
AgeI A'CCGG_T
Cuts at: 4616 4616
Size: 5785
AhdI GACnn_n'nnGTC
Cuts at: 2526 4514 2526
Size: 1988 3797
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5785
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5785
AlwNI CAG_nnn'CTG
Cuts at: 2363 2538 3029 3528 5100 2363
Size: 175 491 499 1572 3048
Fragments arranged by size:
3048 1572 499 491 175
ApaLI G'TGCA_C
Cuts at: 2122 3842 4998 2122
Size: 1720 1156 2909
Fragments arranged by size:
2909 1720 1156
ApoI r'AATT_y
Cuts at: 1493 2281 3094 3320 3472 5581 1493
Size: 788 813 226 152 2109 1697
Fragments arranged by size:
2109 1697 813 788 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4339 160
Size: 872 3307 1606
Fragments arranged by size:
3307 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
AvaI C'yCGr_G
Cuts at: 1870 1943 3001 3110 1870
Size: 73 1058 109 4545
Fragments arranged by size:
4545 1058 109 73
AvrII C'CTAG_G
Cuts at: 2743 2743
Size: 5785
BamHI G'GATC_C
Cuts at: 3115 3482 3115
Size: 367 5418
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2695 3106 4561 618
Size: 325 217 627 908 411 1455 1842
Fragments arranged by size:
1842 1455 908 627 411 325 217
BanII G_rGCy'C
Cuts at: 1391 3104 1391
Size: 1713 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2108 2311 928
Size: 971 209 203 4402
Fragments arranged by size:
4402 971 209 203
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2385 2419 3976 4010 1903
Size: 34 448 34 1557 34 3678
Fragments arranged by size:
3678 1557 448 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3677 4887 3677
Size: 1210 4575
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5785
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2798 4396 136
Size: 107 122 71 631 605 1126 1598 1525
Fragments arranged by size:
1598 1525 1126 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3064 5780 3064
Size: 2716 3069
BlpI GC'TnA_GC
Cuts at: 2579 3202 2579
Size: 623 5162
Bme1580I G_kGCm'C
Cuts at: 1790 2126 2149 2455 3846 5002 1790
Size: 336 23 306 1391 1156 2573
Fragments arranged by size:
2573 1391 1156 336 306 23
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5785
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2777 3516 4474
Size: 416 53 83 399 24 1360 739 958
Cuts at: 4474 442
Size: 1753
Fragments arranged by size:
1753 1360 958 739 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5785
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2941 2941
Size: 5785
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2171 4445 1239
Size: 932 2274 2579
Fragments arranged by size:
2579 2274 932
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3910 4775 5073 3910
Size: 865 298 4622
Fragments arranged by size:
4622 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4448 882
Size: 617 2949 2219
Fragments arranged by size:
2949 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3576 493
Size: 711 2372 2702
Fragments arranged by size:
2702 2372 711
BsaBI GATnn'nnATC
Cuts at: 5779 5779
Size: 5785
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3592 3974 4609 275
Size: 53 83 186 936 2059 382 635 1451
Fragments arranged by size:
2059 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2612 4218 4616 4890 5037 5448 2612
Size: 1606 398 274 147 411 2949
Fragments arranged by size:
2949 1606 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5560
Fragments arranged by size:
5560 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2756 2917 1186
Size: 1570 161 4054
Fragments arranged by size:
4054 1570 161
BseYI C'CCAG_C
Cuts at: 2016 2234 4988 5484 2016
Size: 218 2754 496 2317
Fragments arranged by size:
2754 2317 496 218
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5212
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3235 3996 5024 1540
Size: 275 186 1234 761 1028 2301
Fragments arranged by size:
2301 1234 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2126 2169 2766 3846 3931 5002 2126
Size: 43 597 1080 85 1071 2909
Fragments arranged by size:
2909 1080 1071 597 85 43
BsmI GAATG_Cn'
Cuts at: 2018 3296 3389 2018
Size: 1278 93 4414
Fragments arranged by size:
4414 1278 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2126 2149 2169 2455 2766 3104 3846
Size: 399 336 23 20 286 311 338 742
Cuts at: 3846 3931 5002 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 338 336 311 286
85 23 20
BspEI T'CCGG_A
Cuts at: 2612 5448 2612
Size: 2836 2949
BspHI T'CATG_A
Cuts at: 3674 5404 3674
Size: 1730 4055
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2511 2583 2683 3140 844
Size: 1667 72 100 457 3489
Fragments arranged by size:
3489 1667 457 100 72
BsrBI CCG'CTC
Cuts at: 1394 1823 2925 3672 1394
Size: 429 1102 747 3507
Fragments arranged by size:
3507 1102 747 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4280 4454 65
Size: 1183 3032 174 1396
Fragments arranged by size:
3032 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4429 4616 1700
Size: 110 2619 187 2869
Fragments arranged by size:
2869 2619 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5785
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5785
BssSI C'ACGA_G
Cuts at: 3845 4857 3845
Size: 1012 4773
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5785
BstBI TT'CG_AA
Cuts at: 4589 4589
Size: 5785
BstEII G'GTnAC_C
Cuts at: 1184 2458 1184
Size: 1274 4511
BstYI r'GATC_y
Cuts at: 1972 2919 3005 3064 3115 3482 3867 3884 5336
Size: 947 86 59 51 367 385 17 1452
Cuts at: 5336 5422 5434 5780 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 947 385 367 346 86 86
59 51 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2296 2443 2880 3047 513
Size: 552 939 292 147 437 167 3251
Fragments arranged by size:
3251 939 552 437 292 167 147
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2581 487
Size: 683 56 1355 3691
Fragments arranged by size:
3691 1355 683 56
BtsI GCAGTG_nn'
Cuts at: 2938 3301 4095 4115 2938
Size: 363 794 20 4608
Fragments arranged by size:
4608 794 363 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5785
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5750
DraI TTT'AAA
Cuts at: 3089 3089
Size: 5785
DraIII CAC_nnn'GTG
Cuts at: 3048 3048
Size: 5785
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4792 809
Size: 507 3476 1802
Fragments arranged by size:
3476 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2250 2664 2677
Size: 54 1425 50 275 18 420 414 13
Cuts at: 2677 2733 2811 3050 3232 4121 5750 8
Size: 56 78 239 182 889 1629 43
Fragments arranged by size:
1629 1425 889 420 414 275 239 182
78 56 54 50 43 18 13
EagI C'GGCC_G
Cuts at: 1537 1812 3232 1537
Size: 275 1420 4090
Fragments arranged by size:
4090 1420 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2133 3715 1353
Size: 780 1582 3423
Fragments arranged by size:
3423 1582 780
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2186 4372 4746 4892 387
Size: 1064 138 597 2186 374 146 1280
Fragments arranged by size:
2186 1280 1064 597 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2171 2348 3848 4445 5232 5500 1239
Size: 393 539 177 1500 597 787 268 1524
Fragments arranged by size:
1524 1500 787 597 539 393 268 177
EcoO109I rG'GnC_Cy
Cuts at: 2479 2535 2971 2479
Size: 56 436 5293
Fragments arranged by size:
5293 436 56
EcoRI G'AATT_C
Cuts at: 3094 3472 3094
Size: 378 5407
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2068 2335 2860 2068
Size: 267 525 4993
Fragments arranged by size:
4993 525 267
FspI TGC'GCA
Cuts at: 4291 4291
Size: 5785
HaeII r_GCGC'y
Cuts at: 1678 4932 1678
Size: 3254 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2512 2544 2997 3029 4426
Size: 32 135 32 940 32 453 32 1397
Cuts at: 4426 4458 4500 4532 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1397 940 453 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2887 3129 3375 669
Size: 988 281 949 242 246 3079
Fragments arranged by size:
3079 988 949 281 246 242
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5785
HpaI GTT'AAC
Cuts at: 3375 3375
Size: 5785
KpnI G_GTAC'C
Cuts at: 3110 3110
Size: 5785
MfeI C'AATT_G
Cuts at: 3384 3384
Size: 5785
MluI A'CGCG_T
Cuts at: 5661 5661
Size: 5785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2085 4899 5083 1305
Size: 780 2814 184 2007
Fragments arranged by size:
2814 2007 780 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2252 2666 2679 2735 2813
Size: 54 1425 343 420 414 13 56 78
Cuts at: 2813 3052 10
Size: 239 2743
Fragments arranged by size:
2743 1425 420 414 343 239 78 56
54 13
MslI CAynn'nnrTG
Cuts at: 518 1166 2658 3743 4102 4261 518
Size: 648 1492 1085 359 159 2042
Fragments arranged by size:
2042 1492 1085 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2108 3201 3878 5026 5271 1606
Size: 502 1093 677 1148 245 2120
Fragments arranged by size:
2120 1148 1093 677 502 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2296 2880 513
Size: 552 939 292 584 3418
Fragments arranged by size:
3418 939 584 552 292
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5785
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5785
NotI GC'GGCC_GC
Cuts at: 3232 3232
Size: 5785
NspI r_CATG'y
Cuts at: 1132 1229 2271 2639 2663 3143 4688 1132
Size: 97 1042 368 24 480 1545 2229
Fragments arranged by size:
2229 1545 1042 480 368 97 24
PasI CC'CwG_GG
Cuts at: 1348 2464 1348
Size: 1116 4669
PciI A'CATG_T
Cuts at: 4684 4684
Size: 5785
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2302 2809 1108
Size: 1194 507 4084
Fragments arranged by size:
4084 1194 507
PmeI GTTT'AAAC
Cuts at: 3089 3089
Size: 5785
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3854 3886 5367 5399 3854
Size: 32 1481 32 4240
Fragments arranged by size:
4240 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2479 2479
Size: 5785
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5785
PsiI TTA'TAA
Cuts at: 3355 3355
Size: 5785
PstI C_TGCA'G
Cuts at: 830 3137 830
Size: 2307 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5785
PvuII CAG'CTG
Cuts at: 2108 2108
Size: 5785
SalI G'TCGA_C
Cuts at: 1936 3127 1936
Size: 1191 4594
SbfI CC_TGCA'GG
Cuts at: 3137 3137
Size: 5785
ScaI AGT'ACT
Cuts at: 4033 5745 4033
Size: 1712 4073
SexAI A'CCwGG_T
Cuts at: 2829 2829
Size: 5785
SfcI C'TryA_G
Cuts at: 826 1045 3133 4268 4949 5140 826
Size: 219 2088 1135 681 191 1471
Fragments arranged by size:
2088 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2798 2798
Size: 5785
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5785
SmaI CCC'GGG
Cuts at: 1872 3112 1872
Size: 1240 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3889 4790 5052 820
Size: 197 926 1946 901 262 1553
Fragments arranged by size:
1946 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3576 493
Size: 3083 2702
SpeI A'CTAG_T
Cuts at: 152 2491 152
Size: 2339 3446
SphI G_CATG'C
Cuts at: 1132 2271 3143 1132
Size: 1139 872 3774
Fragments arranged by size:
3774 1139 872
SspI AAT'ATT
Cuts at: 5 52 3709 5
Size: 47 3657 2081
Fragments arranged by size:
3657 2081 47
StuI AGG'CCT
Cuts at: 1517 2843 1517
Size: 1326 4459
StyI C'CwwG_G
Cuts at: 513 1065 2004 2043 2296 2310 2743 2880 2974
Size: 552 939 39 253 14 433 137 94
Cuts at: 2974 3224 513
Size: 250 3074
Fragments arranged by size:
3074 939 552 433 253 250 137 94
39 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2008 2409 3812 3829 3982 4167 4612
Size: 1255 428 401 1403 17 153 185 445
Cuts at: 4612 325
Size: 1498
Fragments arranged by size:
1498 1403 1255 445 428 401 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4031 5743 96
Size: 274 80 33 51 1443 2054 1712 138
Fragments arranged by size:
2054 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2947 2979 5745 5777 2947
Size: 32 2766 32 2955
Fragments arranged by size:
2955 2766 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2831 1316
Size: 1515 4270
XbaI T'CTAG_A
Cuts at: 3067 3121 3067
Size: 54 5731
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2050 2294 2050
Size: 244 5541
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5785
XmaI C'CCGG_G
Cuts at: 1870 3110 1870
Size: 1240 4545
XmnI GAAnn'nnTTC
Cuts at: 3914 3914
Size: 5785
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3593 276
Size: 53 83 186 936 2059 2468
Fragments arranged by size:
2468 2059 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SfoI SrfI SwaI