Restriction map for
pFN21AB7151
(Circular) (Six-base) MAPSORT of: pFN21AB7151.seq Check: 7528 from: 1 to: 5755
pFN21AB7151 5755 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2059 2059
Size: 5755
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3565 278
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3098 1937
Size: 1161 4594
Acc65I G'GTAC_C
Cuts at: 3076 3076
Size: 5755
AclI AA'CG_TT
Cuts at: 1563 3882 4255 1563
Size: 2319 373 3063
Fragments arranged by size:
3063 2319 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2617 3818 5202 5470 1632
Size: 985 1201 1384 268 1917
Fragments arranged by size:
1917 1384 1201 985 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5558
AflIII A'CryG_T
Cuts at: 1203 4654 5631 1203
Size: 3451 977 1327
Fragments arranged by size:
3451 1327 977
AgeI A'CCGG_T
Cuts at: 4586 4586
Size: 5755
AhdI GACnn_n'nnGTC
Cuts at: 4484 4484
Size: 5755
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5755
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5755
AlwNI CAG_nnn'CTG
Cuts at: 2507 3498 5070 2507
Size: 991 1572 3192
Fragments arranged by size:
3192 1572 991
ApaI G_GGCC'C
Cuts at: 2451 2974 2451
Size: 523 5232
ApaLI G'TGCA_C
Cuts at: 3812 4968 3812
Size: 1156 4599
ApoI r'AATT_y
Cuts at: 1493 2014 2300 3064 3290 3442 5551 1493
Size: 521 286 764 226 152 2109 1697
Fragments arranged by size:
2109 1697 764 521 286 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4309 160
Size: 872 3277 1606
Fragments arranged by size:
3277 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
AvaI C'yCGr_G
Cuts at: 1870 1943 2120 3080 1870
Size: 73 177 960 4545
Fragments arranged by size:
4545 960 177 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2487 2520 2487
Size: 33 5722
BamHI G'GATC_C
Cuts at: 3085 3452 3085
Size: 367 5388
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3076 4531 618
Size: 325 217 627 1289 1455 1842
Fragments arranged by size:
1842 1455 1289 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2451 2974 3074 1391
Size: 1060 523 100 4072
Fragments arranged by size:
4072 1060 523 100
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2335 2902 928
Size: 971 436 567 3781
Fragments arranged by size:
3781 971 567 436
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3946 3980 1903
Size: 34 2009 34 3678
Fragments arranged by size:
3678 2009 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3647 4857 3647
Size: 1210 4545
BclI T'GATC_A
Cuts at: 1548 2439 1548
Size: 891 4864
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4366 136
Size: 107 122 71 631 605 2694 1525
Fragments arranged by size:
2694 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5750 5750
Size: 5755
BlpI GC'TnA_GC
Cuts at: 3172 3172
Size: 5755
Bme1580I G_kGCm'C
Cuts at: 1790 2451 2869 2974 3816 4972 1790
Size: 661 418 105 842 1156 2573
Fragments arranged by size:
2573 1156 842 661 418 105
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5755
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3486 4444 442
Size: 416 53 83 399 24 2069 958 1753
Fragments arranged by size:
2069 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5755
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2061 2984 4415 1239
Size: 822 923 1431 2579
Fragments arranged by size:
2579 1431 923 822
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3880 4745 5043 3880
Size: 865 298 4592
Fragments arranged by size:
4592 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4418 882
Size: 617 2919 2219
Fragments arranged by size:
2919 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3546 493
Size: 711 2342 2702
Fragments arranged by size:
2702 2342 711
BsaBI GATnn'nnATC
Cuts at: 5749 5749
Size: 5755
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3562 3944 4579 275
Size: 53 83 186 936 2029 382 635 1451
Fragments arranged by size:
2029 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2163 2616 4188 4586 4860 5007 5418 2163
Size: 453 1572 398 274 147 411 2500
Fragments arranged by size:
2500 1572 453 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5530
Fragments arranged by size:
5530 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2459 1186
Size: 1273 4482
BseYI C'CCAG_C
Cuts at: 4958 5454 4958
Size: 496 5259
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2515 1163
Size: 573 779 4403
Fragments arranged by size:
4403 779 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3205 3966 4994 1540
Size: 275 186 1204 761 1028 2301
Fragments arranged by size:
2301 1204 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3816 3901 4972 3816
Size: 85 1071 4599
Fragments arranged by size:
4599 1071 85
BsmI GAATG_Cn'
Cuts at: 3266 3359 3266
Size: 93 5662
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2451 2869 2974 3074 3816 3901 4972
Size: 399 661 418 105 100 742 85 1071
Cuts at: 4972 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 661 418 399 105 100
85
BspEI T'CCGG_A
Cuts at: 2163 5418 2163
Size: 3255 2500
BspHI T'CATG_A
Cuts at: 3644 5374 3644
Size: 1730 4025
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2059 3110 844
Size: 1215 1051 3489
Fragments arranged by size:
3489 1215 1051
BsrBI CCG'CTC
Cuts at: 1394 1823 3642 1394
Size: 429 1819 3507
Fragments arranged by size:
3507 1819 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2358 2474 4250 4424 65
Size: 1183 1110 116 1776 174 1396
Fragments arranged by size:
1776 1396 1183 1110 174 116
BsrFI r'CCGG_y
Cuts at: 1700 1810 4399 4586 1700
Size: 110 2589 187 2869
Fragments arranged by size:
2869 2589 187 110
BsrGI T'GTAC_A
Cuts at: 96 2829 96
Size: 2733 3022
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5755
BssSI C'ACGA_G
Cuts at: 3815 4827 3815
Size: 1012 4743
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5755
BstBI TT'CG_AA
Cuts at: 4559 4559
Size: 5755
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5755
BstYI r'GATC_y
Cuts at: 1972 2685 3085 3452 3837 3854 5306 5392 5404
Size: 713 400 367 385 17 1452 86 12
Cuts at: 5404 5750 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 713 400 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2208 2262 513
Size: 552 939 204 54 4006
Fragments arranged by size:
4006 939 552 204 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5016
Fragments arranged by size:
5016 683 56
BtsI GCAGTG_nn'
Cuts at: 2856 3271 4065 4085 2856
Size: 415 794 20 4526
Fragments arranged by size:
4526 794 415 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5755
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5720
DraI TTT'AAA
Cuts at: 3059 3059
Size: 5755
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4762 809
Size: 507 3446 1802
Fragments arranged by size:
3446 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2205 2427 2583
Size: 54 1425 50 275 18 375 222 156
Cuts at: 2583 3202 4091 5720 8
Size: 619 889 1629 43
Fragments arranged by size:
1629 1425 889 619 375 275 222 156
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3202 1537
Size: 275 1390 4090
Fragments arranged by size:
4090 1390 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2078 3685 1353
Size: 725 1607 3423
Fragments arranged by size:
3423 1607 725
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4342 4716 4862 387
Size: 1064 138 2753 374 146 1280
Fragments arranged by size:
2753 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2061 2617 2984 3818 4415 5202 5470
Size: 393 429 556 367 834 597 787 268
Cuts at: 5470 1239
Size: 1524
Fragments arranged by size:
1524 834 787 597 556 429 393 367
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2978 2978
Size: 5755
EcoO109I rG'GnC_Cy
Cuts at: 2125 2448 2728 2970 2971 2125
Size: 323 280 242 1 4909
Fragments arranged by size:
4909 323 280 242 1
EcoRI G'AATT_C
Cuts at: 3064 3442 3064
Size: 378 5377
EcoRV GAT'ATC
Cuts at: 2083 2083
Size: 5755
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2716 2716
Size: 5755
FspI TGC'GCA
Cuts at: 4261 4261
Size: 5755
HaeII r_GCGC'y
Cuts at: 1678 4902 1678
Size: 3224 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2598 2630 4396 4428 4470
Size: 32 135 32 1026 32 1766 32 42
Cuts at: 4470 4502 1373
Size: 32 2626
Fragments arranged by size:
2626 1766 1026 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2598 3099 3345 669
Size: 988 281 660 501 246 3079
Fragments arranged by size:
3079 988 660 501 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5755
HpaI GTT'AAC
Cuts at: 3345 3345
Size: 5755
KpnI G_GTAC'C
Cuts at: 3080 3080
Size: 5755
MfeI C'AATT_G
Cuts at: 3354 3354
Size: 5755
MluI A'CGCG_T
Cuts at: 5631 5631
Size: 5755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4869 5053 1305
Size: 3564 184 2007
Fragments arranged by size:
3564 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2429 10
Size: 54 1425 343 597 3336
Fragments arranged by size:
3336 1425 597 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2492 3713 4072 4231 518
Size: 648 1326 1221 359 159 2042
Fragments arranged by size:
2042 1326 1221 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2373 2886 3171 3848 4996 5241 1606
Size: 767 513 285 677 1148 245 2120
Fragments arranged by size:
2120 1148 767 677 513 285 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2262 513
Size: 552 939 258 4006
Fragments arranged by size:
4006 939 552 258
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5755
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5755
NotI GC'GGCC_GC
Cuts at: 3202 3202
Size: 5755
NspI r_CATG'y
Cuts at: 1132 1229 2830 3113 4658 1132
Size: 97 1601 283 1545 2229
Fragments arranged by size:
2229 1601 1545 283 97
PasI CC'CwG_GG
Cuts at: 1348 2451 1348
Size: 1103 4652
PciI A'CATG_T
Cuts at: 4654 4654
Size: 5755
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5755
PfoI T'CCnGG_A
Cuts at: 2076 2928 2076
Size: 852 4903
PmeI GTTT'AAAC
Cuts at: 3059 3059
Size: 5755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3824 3856 5337 5369 3824
Size: 32 1481 32 4210
Fragments arranged by size:
4210 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2125 2728 2125
Size: 603 5152
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5755
PsiI TTA'TAA
Cuts at: 3325 3325
Size: 5755
PspOMI G'GGCC_C
Cuts at: 2447 2970 2447
Size: 523 5232
PstI C_TGCA'G
Cuts at: 830 3034 3107 830
Size: 2204 73 3478
Fragments arranged by size:
3478 2204 73
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5755
PvuII CAG'CTG
Cuts at: 2886 2886
Size: 5755
SalI G'TCGA_C
Cuts at: 1936 3097 1936
Size: 1161 4594
SbfI CC_TGCA'GG
Cuts at: 3107 3107
Size: 5755
ScaI AGT'ACT
Cuts at: 4003 5715 4003
Size: 1712 4043
SexAI A'CCwGG_T
Cuts at: 2506 2506
Size: 5755
SfcI C'TryA_G
Cuts at: 826 1045 2499 3030 3103 4238 4919 5110 826
Size: 219 1454 531 73 1135 681 191 1471
Fragments arranged by size:
1471 1454 1135 681 531 219 191 73
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5755
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5755
SmaI CCC'GGG
Cuts at: 1872 3082 1872
Size: 1210 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3859 4760 5022 820
Size: 197 926 1916 901 262 1553
Fragments arranged by size:
1916 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3546 493
Size: 3053 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5755
SphI G_CATG'C
Cuts at: 1132 3113 1132
Size: 1981 3774
SspI AAT'ATT
Cuts at: 5 52 3679 5
Size: 47 3627 2081
Fragments arranged by size:
3627 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5755
StyI C'CwwG_G
Cuts at: 513 1065 2004 2262 3194 513
Size: 552 939 258 932 3074
Fragments arranged by size:
3074 939 932 552 258
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2140 2443 2656 2993 3782 3799 3952
Size: 1255 560 303 213 337 789 17 153
Cuts at: 3952 4137 4582 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 789 560 445 337 303 213
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2829 4001 5713
Size: 274 80 33 51 1443 852 1172 1712
Cuts at: 5713 96
Size: 138
Fragments arranged by size:
1712 1443 1172 852 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5715 5747 5715
Size: 32 5723
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5755
XbaI T'CTAG_A
Cuts at: 3091 3091
Size: 5755
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5755
XmaI C'CCGG_G
Cuts at: 1870 3080 1870
Size: 1210 4545
XmnI GAAnn'nnTTC
Cuts at: 3884 3884
Size: 5755
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3563 276
Size: 53 83 186 936 2029 2468
Fragments arranged by size:
2468 2029 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI RsrII SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI XcmI