Restriction map for
pFN21AB7144
(Circular) (Six-base) MAPSORT of: pFN21AB7144.seq Check: 8289 from: 1 to: 5722
pFN21AB7144 5722 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3532 278
Size: 53 83 186 936 1996 2468
Fragments arranged by size:
2468 1996 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3065 1937
Size: 1128 4594
Acc65I G'GTAC_C
Cuts at: 3043 3043
Size: 5722
AclI AA'CG_TT
Cuts at: 1563 3849 4222 1563
Size: 2286 373 3063
Fragments arranged by size:
3063 2286 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2360 2404 2916 3785 5169 5437 1632
Size: 728 44 512 869 1384 268 1917
Fragments arranged by size:
1917 1384 869 728 512 268 44
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5525
AflIII A'CryG_T
Cuts at: 1203 2352 4621 5598 1203
Size: 1149 2269 977 1327
Fragments arranged by size:
2269 1327 1149 977
AgeI A'CCGG_T
Cuts at: 4553 4553
Size: 5722
AhdI GACnn_n'nnGTC
Cuts at: 4451 4451
Size: 5722
AleI CACnn'nnGTG
Cuts at: 1166 2090 1166
Size: 924 4798
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2592 2908 1184
Size: 1408 316 3998
Fragments arranged by size:
3998 1408 316
AlwNI CAG_nnn'CTG
Cuts at: 2433 2609 3465 5037 2433
Size: 176 856 1572 3118
Fragments arranged by size:
3118 1572 856 176
ApaLI G'TGCA_C
Cuts at: 3779 4935 3779
Size: 1156 4566
ApoI r'AATT_y
Cuts at: 1493 2096 3031 3257 3409 5518 1493
Size: 603 935 226 152 2109 1697
Fragments arranged by size:
2109 1697 935 603 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4276 160
Size: 872 3244 1606
Fragments arranged by size:
3244 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5722
AvaI C'yCGr_G
Cuts at: 1870 1943 3047 1870
Size: 73 1104 4545
Fragments arranged by size:
4545 1104 73
BamHI G'GATC_C
Cuts at: 2905 3052 3419 2905
Size: 147 367 5208
Fragments arranged by size:
5208 367 147
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3043 4498 618
Size: 325 217 627 1256 1455 1842
Fragments arranged by size:
1842 1455 1256 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2317 3041 1391
Size: 926 724 4072
Fragments arranged by size:
4072 926 724
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2141 2381 2749 928
Size: 971 242 240 368 3901
Fragments arranged by size:
3901 971 368 242 240
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3913 3947 1903
Size: 34 1976 34 3678
Fragments arranged by size:
3678 1976 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2555 3614 4824 2555
Size: 1059 1210 3453
Fragments arranged by size:
3453 1210 1059
BclI T'GATC_A
Cuts at: 1548 2223 2637 1548
Size: 675 414 4633
Fragments arranged by size:
4633 675 414
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4333 136
Size: 107 122 71 631 605 2661 1525
Fragments arranged by size:
2661 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5717 5717
Size: 5722
BlpI GC'TnA_GC
Cuts at: 2610 3139 2610
Size: 529 5193
Bme1580I G_kGCm'C
Cuts at: 1790 3783 4939 1790
Size: 1993 1156 2573
Fragments arranged by size:
2573 1993 1156
BmgBI CAC'GTC
Cuts at: 1318 2379 1318
Size: 1061 4661
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2627 3453 4411
Size: 416 53 83 399 24 1210 826 958
Cuts at: 4411 442
Size: 1753
Fragments arranged by size:
1753 1210 958 826 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5722
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4382 1239
Size: 3143 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2396 2698 3847 4712 5010 2396
Size: 302 1149 865 298 3108
Fragments arranged by size:
3108 1149 865 302 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4385 882
Size: 617 2886 2219
Fragments arranged by size:
2886 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3513 493
Size: 711 2309 2702
Fragments arranged by size:
2702 2309 711
BsaBI GATnn'nnATC
Cuts at: 2642 5716 2642
Size: 3074 2648
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3529 3911 4546 275
Size: 53 83 186 936 1996 382 635 1451
Fragments arranged by size:
1996 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2271 4155 4553 4827 4974 5385 2271
Size: 1884 398 274 147 411 2608
Fragments arranged by size:
2608 1884 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5497
Fragments arranged by size:
5497 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2399 1186
Size: 1213 4509
BseYI C'CCAG_C
Cuts at: 4925 5421 4925
Size: 496 5226
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2184 1163
Size: 573 448 4701
Fragments arranged by size:
4701 573 448
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3172 3933 4961 1540
Size: 275 186 1171 761 1028 2301
Fragments arranged by size:
2301 1171 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3783 3868 4939 3783
Size: 85 1071 4566
Fragments arranged by size:
4566 1071 85
BsmI GAATG_Cn'
Cuts at: 2581 3233 3326 2581
Size: 652 93 4977
Fragments arranged by size:
4977 652 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2317 3041 3783 3868 4939 1391
Size: 399 527 724 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 724 527 399 85
BspEI T'CCGG_A
Cuts at: 2271 5385 2271
Size: 3114 2608
BspHI T'CATG_A
Cuts at: 2220 2988 3611 5341 2220
Size: 768 623 1730 2601
Fragments arranged by size:
2601 1730 768 623
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3077 844
Size: 2233 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2979 3609 1394
Size: 429 1156 630 3507
Fragments arranged by size:
3507 1156 630 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4217 4391 65
Size: 1183 2969 174 1396
Fragments arranged by size:
2969 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4366 4553 1700
Size: 110 2556 187 2869
Fragments arranged by size:
2869 2556 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5722
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5722
BssSI C'ACGA_G
Cuts at: 3782 4794 3782
Size: 1012 4710
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5722
BstBI TT'CG_AA
Cuts at: 4526 4526
Size: 5722
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5722
BstYI r'GATC_y
Cuts at: 1972 2905 3052 3419 3804 3821 5273 5359 5371
Size: 933 147 367 385 17 1452 86 12
Cuts at: 5371 5717 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 933 385 367 346 147 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2805 2959 513
Size: 552 939 801 154 3276
Fragments arranged by size:
3276 939 801 552 154
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2629 487
Size: 683 56 1403 3580
Fragments arranged by size:
3580 1403 683 56
BtsI GCAGTG_nn'
Cuts at: 2061 2726 3238 4032 4052 2061
Size: 665 512 794 20 3731
Fragments arranged by size:
3731 794 665 512 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5722
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2852 2887 2983 3018 529
Size: 35 2288 35 96 35 3233
Fragments arranged by size:
3233 2288 96 35 35 35
DraI TTT'AAA
Cuts at: 3026 3026
Size: 5722
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4729 809
Size: 507 3413 1802
Fragments arranged by size:
3413 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3169 4058 5687
Size: 54 1425 50 275 18 1339 889 1629
Cuts at: 5687 8
Size: 43
Fragments arranged by size:
1629 1425 1339 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3169 1537
Size: 275 1357 4090
Fragments arranged by size:
4090 1357 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3652 1353
Size: 2299 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4309 4683 4829 387
Size: 1064 138 2720 374 146 1280
Fragments arranged by size:
2720 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2360 2404 2916 3785 4382 5169 5437
Size: 393 728 44 512 869 597 787 268
Cuts at: 5437 1239
Size: 1524
Fragments arranged by size:
1524 869 787 728 597 512 393 268
44
EcoRI G'AATT_C
Cuts at: 3031 3409 3031
Size: 378 5344
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2563 2563
Size: 5722
FspI TGC'GCA
Cuts at: 4228 4228
Size: 5722
HaeII r_GCGC'y
Cuts at: 1678 4869 1678
Size: 3191 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2613 2645 2936 2968 4363
Size: 32 135 32 1041 32 291 32 1395
Cuts at: 4363 4395 4437 4469 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1395 1041 291 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3066 3312 669
Size: 988 281 1128 246 3079
Fragments arranged by size:
3079 1128 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5722
HpaI GTT'AAC
Cuts at: 3312 3312
Size: 5722
KpnI G_GTAC'C
Cuts at: 3047 3047
Size: 5722
MfeI C'AATT_G
Cuts at: 3321 3321
Size: 5722
MluI A'CGCG_T
Cuts at: 5598 5598
Size: 5722
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4836 5020 1305
Size: 3531 184 2007
Fragments arranged by size:
3531 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3900
Fragments arranged by size:
3900 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2090 2219 3680 4039 4198 518
Size: 648 924 129 1461 359 159 2042
Fragments arranged by size:
2042 1461 924 648 359 159 129
MspA1I CmG'CkG
Cuts at: 1606 3138 3815 4963 5208 1606
Size: 1532 677 1148 245 2120
Fragments arranged by size:
2120 1532 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2805 2959 513
Size: 552 939 801 154 3276
Fragments arranged by size:
3276 939 801 552 154
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5722
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5722
NotI GC'GGCC_GC
Cuts at: 3169 3169
Size: 5722
NspI r_CATG'y
Cuts at: 1132 1229 2356 3080 4625 1132
Size: 97 1127 724 1545 2229
Fragments arranged by size:
2229 1545 1127 724 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5722
PciI A'CATG_T
Cuts at: 2352 4621 2352
Size: 2269 3453
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2433 1108
Size: 1325 4397
PmeI GTTT'AAAC
Cuts at: 3026 3026
Size: 5722
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3791 3823 5304 5336 3791
Size: 32 1481 32 4177
Fragments arranged by size:
4177 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5722
PsiI TTA'TAA
Cuts at: 3292 3292
Size: 5722
PstI C_TGCA'G
Cuts at: 830 2755 3074 830
Size: 1925 319 3478
Fragments arranged by size:
3478 1925 319
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5722
SalI G'TCGA_C
Cuts at: 1936 3064 1936
Size: 1128 4594
SbfI CC_TGCA'GG
Cuts at: 3074 3074
Size: 5722
ScaI AGT'ACT
Cuts at: 3970 5682 3970
Size: 1712 4010
SfcI C'TryA_G
Cuts at: 826 1045 2751 3070 4205 4886 5077 826
Size: 219 1706 319 1135 681 191 1471
Fragments arranged by size:
1706 1471 1135 681 319 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5722
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5722
SmaI CCC'GGG
Cuts at: 1872 3049 1872
Size: 1177 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2411 2677 3826 4727 4989 820
Size: 197 926 468 266 1149 901 262 1553
Fragments arranged by size:
1553 1149 926 901 468 266 262 197
SnaBI TAC'GTA
Cuts at: 493 3513 493
Size: 3020 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5722
SphI G_CATG'C
Cuts at: 1132 3080 1132
Size: 1948 3774
SspI AAT'ATT
Cuts at: 5 52 3646 5
Size: 47 3594 2081
Fragments arranged by size:
3594 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5722
StyI C'CwwG_G
Cuts at: 513 1065 2004 2701 2805 2959 3161 513
Size: 552 939 697 104 154 202 3074
Fragments arranged by size:
3074 939 697 552 202 154 104
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3749 3766 3919 4104 4549 325
Size: 1255 2169 17 153 185 445 1498
Fragments arranged by size:
2169 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3968 5680 96
Size: 274 80 33 51 1443 1991 1712 138
Fragments arranged by size:
1991 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2844 2876 5682 5714 2844
Size: 32 2806 32 2852
Fragments arranged by size:
2852 2806 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5722
XbaI T'CTAG_A
Cuts at: 3058 3058
Size: 5722
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5722
XmaI C'CCGG_G
Cuts at: 1870 3047 1870
Size: 1177 4545
XmnI GAAnn'nnTTC
Cuts at: 3851 3851
Size: 5722
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3530 276
Size: 53 83 186 936 1996 2468
Fragments arranged by size:
2468 1996 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI