Restriction map for
pFN21AB7138
(Circular) (Six-base) MAPSORT of: pFN21AB7138.seq Check: 3422 from: 1 to: 5698
pFN21AB7138 5698 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2525 2713 2525
Size: 188 5510
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2663 3508 278
Size: 53 83 186 936 1127 845 2468
Fragments arranged by size:
2468 1127 936 845 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2962 3041 1937
Size: 1025 79 4594
Fragments arranged by size:
4594 1025 79
Acc65I G'GTAC_C
Cuts at: 3019 3019
Size: 5698
AclI AA'CG_TT
Cuts at: 1563 3825 4198 1563
Size: 2262 373 3063
Fragments arranged by size:
3063 2262 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3761 5145 5413 1632
Size: 2129 1384 268 1917
Fragments arranged by size:
2129 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5501
AflIII A'CryG_T
Cuts at: 1203 2406 2840 4597 5574 1203
Size: 1203 434 1757 977 1327
Fragments arranged by size:
1757 1327 1203 977 434
AgeI A'CCGG_T
Cuts at: 4529 4529
Size: 5698
AhdI GACnn_n'nnGTC
Cuts at: 4427 4427
Size: 5698
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5698
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2435 1184
Size: 1251 4447
AlwNI CAG_nnn'CTG
Cuts at: 3441 5013 3441
Size: 1572 4126
ApaLI G'TGCA_C
Cuts at: 3755 4911 3755
Size: 1156 4542
ApoI r'AATT_y
Cuts at: 1493 3007 3233 3385 5494 1493
Size: 1514 226 152 2109 1697
Fragments arranged by size:
2109 1697 1514 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4252 160
Size: 872 3220 1606
Fragments arranged by size:
3220 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
AvaI C'yCGr_G
Cuts at: 1870 1943 2889 3023 1870
Size: 73 946 134 4545
Fragments arranged by size:
4545 946 134 73
BamHI G'GATC_C
Cuts at: 3028 3395 3028
Size: 367 5331
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3019 4474 618
Size: 325 217 627 1232 1455 1842
Fragments arranged by size:
1842 1455 1232 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2302 3017 1391
Size: 911 715 4072
Fragments arranged by size:
4072 911 715
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2077 928
Size: 971 178 4549
Fragments arranged by size:
4549 971 178
BbvCI CC'TCA_GC
Cuts at: 2834 2834
Size: 5698
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3889 3923 1903
Size: 34 1952 34 3678
Fragments arranged by size:
3678 1952 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3590 4800 3590
Size: 1210 4488
BclI T'GATC_A
Cuts at: 1548 2007 1548
Size: 459 5239
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2423 4309 136
Size: 107 122 71 631 605 751 1886 1525
Fragments arranged by size:
1886 1525 751 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5693 5693
Size: 5698
BlpI GC'TnA_GC
Cuts at: 3115 3115
Size: 5698
Bme1580I G_kGCm'C
Cuts at: 1790 3759 4915 1790
Size: 1969 1156 2573
Fragments arranged by size:
2573 1969 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5698
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3429 4387 442
Size: 416 53 83 399 24 2012 958 1753
Fragments arranged by size:
2012 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5698
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4358 1239
Size: 3119 2579
Bpu10I CC'TnA_GC
Cuts at: 2834 2834
Size: 5698
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2507 3823 4688 4986 2507
Size: 1316 865 298 3219
Fragments arranged by size:
3219 1316 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4361 882
Size: 617 2862 2219
Fragments arranged by size:
2862 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2409 2841 3489 493
Size: 711 1205 432 648 2702
Fragments arranged by size:
2702 1205 711 648 432
BsaBI GATnn'nnATC
Cuts at: 2705 5692 2705
Size: 2987 2711
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2660 3505 3887 4522
Size: 53 83 186 936 1127 845 382 635
Cuts at: 4522 275
Size: 1451
Fragments arranged by size:
1451 1127 936 845 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4131 4529 4803 4950 5361 4131
Size: 398 274 147 411 4468
Fragments arranged by size:
4468 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5473
Fragments arranged by size:
5473 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5698
BseYI C'CCAG_C
Cuts at: 2773 4901 5397 2773
Size: 2128 496 3074
Fragments arranged by size:
3074 2128 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2741 1163
Size: 573 1005 4120
Fragments arranged by size:
4120 1005 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3148 3909 4937 1540
Size: 275 186 1147 761 1028 2301
Fragments arranged by size:
2301 1147 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2302 2730 3759 3844 4915 2302
Size: 428 1029 85 1071 3085
Fragments arranged by size:
3085 1071 1029 428 85
BsmI GAATG_Cn'
Cuts at: 3209 3302 3209
Size: 93 5605
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2302 2730 3017 3759 3844 4915 1391
Size: 399 512 428 287 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 512 428 399 287 85
BspEI T'CCGG_A
Cuts at: 5361 5361
Size: 5698
BspHI T'CATG_A
Cuts at: 2610 2826 3587 5317 2610
Size: 216 761 1730 2991
Fragments arranged by size:
2991 1730 761 216
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2525 2713 3053 844
Size: 1681 188 340 3489
Fragments arranged by size:
3489 1681 340 188
BsrBI CCG'CTC
Cuts at: 1394 1823 3585 1394
Size: 429 1762 3507
Fragments arranged by size:
3507 1762 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2322 4193 4367 65
Size: 1183 1074 1871 174 1396
Fragments arranged by size:
1871 1396 1183 1074 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4342 4529 1700
Size: 110 2532 187 2869
Fragments arranged by size:
2869 2532 187 110
BsrGI T'GTAC_A
Cuts at: 96 2374 96
Size: 2278 3420
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5698
BssSI C'ACGA_G
Cuts at: 3758 4770 3758
Size: 1012 4686
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5698
BstBI TT'CG_AA
Cuts at: 2236 2926 4502 2236
Size: 690 1576 3432
Fragments arranged by size:
3432 1576 690
BstEII G'GTnAC_C
Cuts at: 1184 2173 1184
Size: 989 4709
BstXI CCAn_nnnn'nTGG
Cuts at: 2294 2294
Size: 5698
BstYI r'GATC_y
Cuts at: 1972 3028 3395 3780 3797 5249 5335 5347 5693
Size: 1056 367 385 17 1452 86 12 346
Cuts at: 5693 1972
Size: 1977
Fragments arranged by size:
1977 1452 1056 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2169 513
Size: 552 1104 4042
Fragments arranged by size:
4042 1104 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4959
Fragments arranged by size:
4959 683 56
BtsI GCAGTG_nn'
Cuts at: 2127 3214 4008 4028 2127
Size: 1087 794 20 3797
Fragments arranged by size:
3797 1087 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5698
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5663
DraI TTT'AAA
Cuts at: 3002 3002
Size: 5698
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4705 809
Size: 507 3389 1802
Fragments arranged by size:
3389 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2264 2367 3145
Size: 54 1425 50 275 18 434 103 778
Cuts at: 3145 4034 5663 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 778 434 275 103 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3145 1537
Size: 275 1333 4090
Fragments arranged by size:
4090 1333 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2156 3628 1353
Size: 803 1472 3423
Fragments arranged by size:
3423 1472 803
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4285 4659 4805 387
Size: 1064 138 2696 374 146 1280
Fragments arranged by size:
2696 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2300 2300
Size: 5698
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3761 4358 5145 5413 1239
Size: 393 2129 597 787 268 1524
Fragments arranged by size:
2129 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3007 3385 3007
Size: 378 5320
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2343 2343
Size: 5698
FspI TGC'GCA
Cuts at: 2936 4204 2936
Size: 1268 4430
HaeII r_GCGC'y
Cuts at: 1678 4845 1678
Size: 3167 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2452 2484 2486 2518 4339
Size: 32 135 32 880 32 2 32 1821
Cuts at: 4339 4371 4413 4445 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1821 880 135 42 32 32 32
32 32 32 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2122 3042 3288 669
Size: 988 281 184 920 246 3079
Fragments arranged by size:
3079 988 920 281 246 184
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5698
HpaI GTT'AAC
Cuts at: 2122 3288 2122
Size: 1166 4532
KpnI G_GTAC'C
Cuts at: 3023 3023
Size: 5698
MfeI C'AATT_G
Cuts at: 3297 3297
Size: 5698
MluI A'CGCG_T
Cuts at: 5574 5574
Size: 5698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4812 4996 1305
Size: 3507 184 2007
Fragments arranged by size:
3507 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2266 2369 10
Size: 54 1425 343 434 103 3339
Fragments arranged by size:
3339 1425 434 343 103 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2058 2183 3656 4015 4174 518
Size: 648 892 125 1473 359 159 2042
Fragments arranged by size:
2042 1473 892 648 359 159 125
MspA1I CmG'CkG
Cuts at: 1606 3114 3791 4939 5184 1606
Size: 1508 677 1148 245 2120
Fragments arranged by size:
2120 1508 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5146
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5698
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5698
NotI GC'GGCC_GC
Cuts at: 3145 3145
Size: 5698
NspI r_CATG'y
Cuts at: 1132 1229 2935 3056 4601 1132
Size: 97 1706 121 1545 2229
Fragments arranged by size:
2229 1706 1545 121 97
PasI CC'CwG_GG
Cuts at: 1348 2802 1348
Size: 1454 4244
PciI A'CATG_T
Cuts at: 4597 4597
Size: 5698
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5698
PmeI GTTT'AAAC
Cuts at: 3002 3002
Size: 5698
PmlI CAC'GTG
Cuts at: 2409 2841 2409
Size: 432 5266
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3767 3799 5280 5312 3767
Size: 32 1481 32 4153
Fragments arranged by size:
4153 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5698
PsiI TTA'TAA
Cuts at: 2219 3268 2219
Size: 1049 4649
PspXI vC'TCGA_Gb
Cuts at: 2889 2889
Size: 5698
PstI C_TGCA'G
Cuts at: 830 3050 830
Size: 2220 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5698
SacI G_AGCT'C
Cuts at: 2302 2302
Size: 5698
SalI G'TCGA_C
Cuts at: 1936 3040 1936
Size: 1104 4594
SbfI CC_TGCA'GG
Cuts at: 3050 3050
Size: 5698
ScaI AGT'ACT
Cuts at: 2279 3946 5658 2279
Size: 1667 1712 2319
Fragments arranged by size:
2319 1712 1667
SfcI C'TryA_G
Cuts at: 826 1045 2089 3046 4181 4862 5053 826
Size: 219 1044 957 1135 681 191 1471
Fragments arranged by size:
1471 1135 1044 957 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5698
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5698
SmaI CCC'GGG
Cuts at: 1872 3025 1872
Size: 1153 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2522 2889 3802 4703 4965 820
Size: 197 926 579 367 913 901 262 1553
Fragments arranged by size:
1553 926 913 901 579 367 262 197
SnaBI TAC'GTA
Cuts at: 493 3489 493
Size: 2996 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5698
SphI G_CATG'C
Cuts at: 1132 2935 3056 1132
Size: 1803 121 3774
Fragments arranged by size:
3774 1803 121
SspI AAT'ATT
Cuts at: 5 52 3622 5
Size: 47 3570 2081
Fragments arranged by size:
3570 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5698
StyI C'CwwG_G
Cuts at: 513 1065 3137 513
Size: 552 2072 3074
Fragments arranged by size:
3074 2072 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2607 3725 3742 3895 4080 4525 325
Size: 1255 1027 1118 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1118 1027 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2277 2374 3944
Size: 274 80 33 51 1443 300 97 1570
Cuts at: 3944 5656 96
Size: 1712 138
Fragments arranged by size:
1712 1570 1443 300 274 138 97 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5658 5690 5658
Size: 32 5666
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5698
XbaI T'CTAG_A
Cuts at: 3034 3034
Size: 5698
XhoI C'TCGA_G
Cuts at: 1943 2889 1943
Size: 946 4752
XmaI C'CCGG_G
Cuts at: 1870 3023 1870
Size: 1153 4545
XmnI GAAnn'nnTTC
Cuts at: 3827 3827
Size: 5698
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2661 3506 276
Size: 53 83 186 936 1127 845 2468
Fragments arranged by size:
2468 1127 936 845 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspXI PstI PvuI SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PpuMI PspOMI PsrI PvuII RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI