Restriction map for
pFN21AB7137
(Circular) (Six-base) MAPSORT of: pFN21AB7137.seq Check: 2229 from: 1 to: 5689
pFN21AB7137 5689 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2786 2786
Size: 5689
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3499 278
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3032 1937
Size: 1095 4594
Acc65I G'GTAC_C
Cuts at: 3010 3010
Size: 5689
AclI AA'CG_TT
Cuts at: 1563 3816 4189 1563
Size: 2253 373 3063
Fragments arranged by size:
3063 2253 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2756 2965 3752 5136 5404 1632
Size: 1124 209 787 1384 268 1917
Fragments arranged by size:
1917 1384 1124 787 268 209
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5492
AflIII A'CryG_T
Cuts at: 1203 4588 5565 1203
Size: 3385 977 1327
Fragments arranged by size:
3385 1327 977
AgeI A'CCGG_T
Cuts at: 2421 4520 2421
Size: 2099 3590
AhdI GACnn_n'nnGTC
Cuts at: 4418 4418
Size: 5689
AleI CACnn'nnGTG
Cuts at: 1166 2498 2798 1166
Size: 1332 300 4057
Fragments arranged by size:
4057 1332 300
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2452 1184
Size: 1268 4421
AlwNI CAG_nnn'CTG
Cuts at: 3432 5004 3432
Size: 1572 4117
ApaI G_GGCC'C
Cuts at: 2173 2173
Size: 5689
ApaLI G'TGCA_C
Cuts at: 3746 4902 3746
Size: 1156 4533
ApoI r'AATT_y
Cuts at: 1493 2998 3224 3376 5485 1493
Size: 1505 226 152 2109 1697
Fragments arranged by size:
2109 1697 1505 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4243 160
Size: 872 3211 1606
Fragments arranged by size:
3211 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
AvaI C'yCGr_G
Cuts at: 1870 1943 3014 1870
Size: 73 1071 4545
Fragments arranged by size:
4545 1071 73
BamHI G'GATC_C
Cuts at: 3019 3386 3019
Size: 367 5322
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3010 4465 618
Size: 325 217 627 1223 1455 1842
Fragments arranged by size:
1842 1455 1223 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2173 2223 2330 3008 1391
Size: 782 50 107 678 4072
Fragments arranged by size:
4072 782 678 107 50
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2750 2815 928
Size: 971 851 65 3802
Fragments arranged by size:
3802 971 851 65
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3880 3914 1903
Size: 34 1943 34 3678
Fragments arranged by size:
3678 1943 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3581 4791 3581
Size: 1210 4479
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5689
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4300 136
Size: 107 122 71 631 605 2628 1525
Fragments arranged by size:
2628 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5684 5684
Size: 5689
BlpI GC'TnA_GC
Cuts at: 3106 3106
Size: 5689
Bme1580I G_kGCm'C
Cuts at: 1790 2173 3750 4906 1790
Size: 383 1577 1156 2573
Fragments arranged by size:
2573 1577 1156 383
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5689
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3420 4378 442
Size: 416 53 83 399 24 2003 958 1753
Fragments arranged by size:
2003 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5689
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2925 2925
Size: 5689
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2710 4349 1239
Size: 1471 1639 2579
Fragments arranged by size:
2579 1639 1471
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3814 4679 4977 3814
Size: 865 298 4526
Fragments arranged by size:
4526 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2851 4352 882
Size: 617 1352 1501 2219
Fragments arranged by size:
2219 1501 1352 617
BsaAI yAC'GTr
Cuts at: 493 1204 2373 3480 493
Size: 711 1169 1107 2702
Fragments arranged by size:
2702 1169 1107 711
BsaBI GATnn'nnATC
Cuts at: 2699 5683 2699
Size: 2984 2705
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3496 3878 4513 275
Size: 53 83 186 936 1963 382 635 1451
Fragments arranged by size:
1963 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2421 4122 4520 4794 4941 5352 2421
Size: 1701 398 274 147 411 2758
Fragments arranged by size:
2758 1701 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5464
Fragments arranged by size:
5464 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2390 1186
Size: 1204 4485
BseYI C'CCAG_C
Cuts at: 2051 2355 4892 5388 2051
Size: 304 2537 496 2352
Fragments arranged by size:
2537 2352 496 304
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2260 1163
Size: 573 524 4592
Fragments arranged by size:
4592 573 524
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3139 3900 4928 1540
Size: 275 186 1138 761 1028 2301
Fragments arranged by size:
2301 1138 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2118 2330 3750 3835 4906 2118
Size: 212 1420 85 1071 2901
Fragments arranged by size:
2901 1420 1071 212 85
BsmI GAATG_Cn'
Cuts at: 3200 3293 3200
Size: 93 5596
BsmBI CGTCTCn'nnnn_
Cuts at: 2728 2728
Size: 5689
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2118 2173 2223 2330 3008 3750 3835
Size: 399 328 55 50 107 678 742 85
Cuts at: 3835 4906 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 678 399 328 107 85
55 50
BspEI T'CCGG_A
Cuts at: 5352 5352
Size: 5689
BspHI T'CATG_A
Cuts at: 2649 3578 5308 2649
Size: 929 1730 3030
Fragments arranged by size:
3030 1730 929
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2786 3044 844
Size: 1942 258 3489
Fragments arranged by size:
3489 1942 258
BsrBI CCG'CTC
Cuts at: 1394 1823 3576 1394
Size: 429 1753 3507
Fragments arranged by size:
3507 1753 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4184 4358 65
Size: 1183 2936 174 1396
Fragments arranged by size:
2936 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2421 4333 4520 1700
Size: 110 611 1912 187 2869
Fragments arranged by size:
2869 1912 611 187 110
BsrGI T'GTAC_A
Cuts at: 96 2889 96
Size: 2793 2896
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5689
BssSI C'ACGA_G
Cuts at: 3749 4761 3749
Size: 1012 4677
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5689
BstBI TT'CG_AA
Cuts at: 4493 4493
Size: 5689
BstEII G'GTnAC_C
Cuts at: 1184 2083 1184
Size: 899 4790
BstXI CCAn_nnnn'nTGG
Cuts at: 2302 2302
Size: 5689
BstYI r'GATC_y
Cuts at: 1972 3019 3386 3771 3788 5240 5326 5338 5684
Size: 1047 367 385 17 1452 86 12 346
Cuts at: 5684 1972
Size: 1977
Fragments arranged by size:
1977 1452 1047 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2960 2960
Size: 5689
BtgI C'CryG_G
Cuts at: 513 1065 2796 513
Size: 552 1731 3406
Fragments arranged by size:
3406 1731 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2686 487
Size: 683 56 1460 3490
Fragments arranged by size:
3490 1460 683 56
BtsI GCAGTG_nn'
Cuts at: 3205 3999 4019 3205
Size: 794 20 4875
Fragments arranged by size:
4875 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5689
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5654
DraI TTT'AAA
Cuts at: 2993 2993
Size: 5689
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2704 4696 809
Size: 507 1388 1992 1802
Fragments arranged by size:
1992 1802 1388 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2199 2304 2490
Size: 54 1425 50 275 18 369 105 186
Cuts at: 2490 2526 3136 4025 5654 8
Size: 36 610 889 1629 43
Fragments arranged by size:
1629 1425 889 610 369 275 186 105
54 50 43 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 3136 1537
Size: 275 1324 4090
Fragments arranged by size:
4090 1324 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2159 2287 2316 2788 2861 3619 1353
Size: 806 128 29 472 73 758 3423
Fragments arranged by size:
3423 806 758 472 128 73 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2678 4276 4650 4796 387
Size: 1064 138 1089 1598 374 146 1280
Fragments arranged by size:
1598 1280 1089 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2328 2328
Size: 5689
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2710 2756 2965 3752 4349 5136 5404
Size: 393 1078 46 209 787 597 787 268
Cuts at: 5404 1239
Size: 1524
Fragments arranged by size:
1524 1078 787 787 597 393 268 209
46
EcoNI CCTnn'n_nnAGG
Cuts at: 2755 2755
Size: 5689
EcoO109I rG'GnC_Cy
Cuts at: 2169 2170 2599 2169
Size: 1 429 5259
Fragments arranged by size:
5259 429 1
EcoRI G'AATT_C
Cuts at: 2998 3376 2998
Size: 378 5311
FspI TGC'GCA
Cuts at: 4195 4195
Size: 5689
HaeII r_GCGC'y
Cuts at: 1678 2303 4836 1678
Size: 625 2533 2531
Fragments arranged by size:
2533 2531 625
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2008 2040 4330 4362 4404
Size: 32 135 32 436 32 2290 32 42
Cuts at: 4404 4436 1373
Size: 32 2626
Fragments arranged by size:
2626 2290 436 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2521 2641 3033 3279 669
Size: 988 281 583 120 392 246 3079
Fragments arranged by size:
3079 988 583 392 281 246 120
HindIII A'AGCT_T
Cuts at: 748 2966 748
Size: 2218 3471
HpaI GTT'AAC
Cuts at: 3279 3279
Size: 5689
KpnI G_GTAC'C
Cuts at: 3014 3014
Size: 5689
MfeI C'AATT_G
Cuts at: 3288 3288
Size: 5689
MluI A'CGCG_T
Cuts at: 5565 5565
Size: 5689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2298 4803 4987 1305
Size: 993 2505 184 2007
Fragments arranged by size:
2505 2007 993 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2201 2306 2492 10
Size: 54 1425 343 369 105 186 3207
Fragments arranged by size:
3207 1425 369 343 186 105 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2408 2498 2798 3647 4006 4165 518
Size: 648 1242 90 300 849 359 159 2042
Fragments arranged by size:
2042 1242 849 648 359 300 159 90
MspA1I CmG'CkG
Cuts at: 1606 2145 2446 2484 2819 3105 3782 4930 5175
Size: 539 301 38 335 286 677 1148 245
Cuts at: 5175 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 539 335 301 286 245
38
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5137
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5689
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5689
NotI GC'GGCC_GC
Cuts at: 3136 3136
Size: 5689
NspI r_CATG'y
Cuts at: 1132 1229 2809 3047 4592 1132
Size: 97 1580 238 1545 2229
Fragments arranged by size:
2229 1580 1545 238 97
PasI CC'CwG_GG
Cuts at: 1348 2216 1348
Size: 868 4821
PciI A'CATG_T
Cuts at: 4588 4588
Size: 5689
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2080 1108
Size: 972 4717
PmeI GTTT'AAAC
Cuts at: 2993 2993
Size: 5689
PmlI CAC'GTG
Cuts at: 2373 2373
Size: 5689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3758 3790 5271 5303 3758
Size: 32 1481 32 4144
Fragments arranged by size:
4144 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2599 2599
Size: 5689
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5689
PsiI TTA'TAA
Cuts at: 3259 3259
Size: 5689
PspOMI G'GGCC_C
Cuts at: 2169 2169
Size: 5689
PstI C_TGCA'G
Cuts at: 830 2103 2483 2627 3041 830
Size: 1273 380 144 414 3478
Fragments arranged by size:
3478 1273 414 380 144
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5689
PvuII CAG'CTG
Cuts at: 2145 2145
Size: 5689
SacI G_AGCT'C
Cuts at: 2330 2330
Size: 5689
SalI G'TCGA_C
Cuts at: 1936 3031 1936
Size: 1095 4594
SapI GCTCTTCn'nnn_
Cuts at: 2788 2861 2788
Size: 73 5616
SbfI CC_TGCA'GG
Cuts at: 2103 3041 2103
Size: 938 4751
ScaI AGT'ACT
Cuts at: 3937 5649 3937
Size: 1712 3977
SfcI C'TryA_G
Cuts at: 826 1045 2099 2479 2623 2854 3037 4172 4853
Size: 219 1054 380 144 231 183 1135 681
Cuts at: 4853 5044 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1054 681 380 231 219 191
183 144
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5689
SmaI CCC'GGG
Cuts at: 1872 3016 1872
Size: 1144 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3793 4694 4956 820
Size: 197 926 1850 901 262 1553
Fragments arranged by size:
1850 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3480 493
Size: 2987 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5689
SphI G_CATG'C
Cuts at: 1132 3047 1132
Size: 1915 3774
SspI AAT'ATT
Cuts at: 5 52 3613 5
Size: 47 3561 2081
Fragments arranged by size:
3561 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5689
StyI C'CwwG_G
Cuts at: 513 1065 2057 2196 2295 3128 513
Size: 552 992 139 99 833 3074
Fragments arranged by size:
3074 992 833 552 139 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2133 3716 3733 3886 4071 4516 325
Size: 1255 553 1583 17 153 185 445 1498
Fragments arranged by size:
1583 1498 1255 553 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2235 2889 3935
Size: 274 80 33 51 1443 258 654 1046
Cuts at: 3935 5647 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1046 654 274 258 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5649 5681 5649
Size: 32 5657
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5689
XbaI T'CTAG_A
Cuts at: 3025 3025
Size: 5689
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5689
XmaI C'CCGG_G
Cuts at: 1870 3014 1870
Size: 1144 4545
XmnI GAAnn'nnTTC
Cuts at: 2768 2969 3818 2768
Size: 201 849 4639
Fragments arranged by size:
4639 849 201
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3497 276
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI