Restriction map for
pFN21AB7134
(Circular) (Six-base) MAPSORT of: pFN21AB7134.seq Check: 9939 from: 1 to: 5665
pFN21AB7134 5665 bp
With 182 enzymes: SgfI *
October 20, 2009 10:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3475 278
Size: 53 83 186 936 1939 2468
Fragments arranged by size:
2468 1939 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3008 1937
Size: 1071 4594
Acc65I G'GTAC_C
Cuts at: 2986 2986
Size: 5665
AclI AA'CG_TT
Cuts at: 1563 3792 4165 1563
Size: 2229 373 3063
Fragments arranged by size:
3063 2229 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3728 5112 5380 1632
Size: 2096 1384 268 1917
Fragments arranged by size:
2096 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5468
AflIII A'CryG_T
Cuts at: 1203 4564 5541 1203
Size: 3361 977 1327
Fragments arranged by size:
3361 1327 977
AgeI A'CCGG_T
Cuts at: 4496 4496
Size: 5665
AhdI GACnn_n'nnGTC
Cuts at: 4394 4394
Size: 5665
AleI CACnn'nnGTG
Cuts at: 1166 2036 2736 1166
Size: 870 700 4095
Fragments arranged by size:
4095 870 700
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5665
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2621 2653 2621
Size: 32 5633
AlwNI CAG_nnn'CTG
Cuts at: 3408 4980 3408
Size: 1572 4093
ApaLI G'TGCA_C
Cuts at: 3722 4878 3722
Size: 1156 4509
ApoI r'AATT_y
Cuts at: 1493 2974 3200 3352 5461 1493
Size: 1481 226 152 2109 1697
Fragments arranged by size:
2109 1697 1481 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4219 160
Size: 872 3187 1606
Fragments arranged by size:
3187 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5665
AvaI C'yCGr_G
Cuts at: 1870 1943 2268 2990 1870
Size: 73 325 722 4545
Fragments arranged by size:
4545 722 325 73
BamHI G'GATC_C
Cuts at: 2456 2995 3362 2456
Size: 539 367 4759
Fragments arranged by size:
4759 539 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2879 2986 4441 618
Size: 325 217 627 1092 107 1455 1842
Fragments arranged by size:
1842 1455 1092 627 325 217 107
BanII G_rGCy'C
Cuts at: 1391 2984 1391
Size: 1593 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2131 928
Size: 971 232 4462
Fragments arranged by size:
4462 971 232
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3856 3890 1903
Size: 34 1919 34 3678
Fragments arranged by size:
3678 1919 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2060 3557 4767 2060
Size: 1497 1210 2958
Fragments arranged by size:
2958 1497 1210
BclI T'GATC_A
Cuts at: 1548 2487 1548
Size: 939 4726
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4276 136
Size: 107 122 71 631 605 2604 1525
Fragments arranged by size:
2604 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5660 5660
Size: 5665
BlpI GC'TnA_GC
Cuts at: 3082 3082
Size: 5665
Bme1580I G_kGCm'C
Cuts at: 1790 3726 4882 1790
Size: 1936 1156 2573
Fragments arranged by size:
2573 1936 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5665
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3396 4354 442
Size: 416 53 83 399 24 1979 958 1753
Fragments arranged by size:
1979 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5665
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4325 1239
Size: 3086 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3790 4655 4953 3790
Size: 865 298 4502
Fragments arranged by size:
4502 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4328 882
Size: 617 2829 2219
Fragments arranged by size:
2829 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3456 493
Size: 711 2252 2702
Fragments arranged by size:
2702 2252 711
BsaBI GATnn'nnATC
Cuts at: 2435 5659 2435
Size: 3224 2441
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3472 3854 4489 275
Size: 53 83 186 936 1939 382 635 1451
Fragments arranged by size:
1939 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4098 4496 4770 4917 5328 4098
Size: 398 274 147 411 4435
Fragments arranged by size:
4435 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2949 2979 1178
Size: 30 165 30 1546 30 3864
Fragments arranged by size:
3864 1546 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5665
BseYI C'CCAG_C
Cuts at: 4868 5364 4868
Size: 496 5169
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2150 1163
Size: 573 414 4678
Fragments arranged by size:
4678 573 414
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3115 3876 4904 1540
Size: 275 186 1114 761 1028 2301
Fragments arranged by size:
2301 1114 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2409 3726 3811 4882 2409
Size: 1317 85 1071 3192
Fragments arranged by size:
3192 1317 1071 85
BsmI GAATG_Cn'
Cuts at: 3176 3269 3176
Size: 93 5572
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2409 2984 3726 3811 4882 1391
Size: 399 619 575 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 619 575 399 85
BspEI T'CCGG_A
Cuts at: 5328 5328
Size: 5665
BspHI T'CATG_A
Cuts at: 3554 5284 3554
Size: 1730 3935
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2850 3020 844
Size: 2006 170 3489
Fragments arranged by size:
3489 2006 170
BsrBI CCG'CTC
Cuts at: 1394 1823 2701 3552 1394
Size: 429 878 851 3507
Fragments arranged by size:
3507 878 851 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2121 4160 4334 65
Size: 1183 873 2039 174 1396
Fragments arranged by size:
2039 1396 1183 873 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4309 4496 1700
Size: 110 2499 187 2869
Fragments arranged by size:
2869 2499 187 110
BsrGI T'GTAC_A
Cuts at: 96 2782 96
Size: 2686 2979
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5665
BssSI C'ACGA_G
Cuts at: 3725 4737 3725
Size: 1012 4653
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5665
BstBI TT'CG_AA
Cuts at: 4469 4469
Size: 5665
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5665
BstXI CCAn_nnnn'nTGG
Cuts at: 2212 2347 2614 2212
Size: 135 267 5263
Fragments arranged by size:
5263 267 135
BstYI r'GATC_y
Cuts at: 1972 2241 2456 2995 3362 3747 3764 5216 5302
Size: 269 215 539 367 385 17 1452 86
Cuts at: 5302 5314 5660 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 539 385 367 346 269 215
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2352 2452 513
Size: 552 1287 100 3726
Fragments arranged by size:
3726 1287 552 100
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4926
Fragments arranged by size:
4926 683 56
BtsI GCAGTG_nn'
Cuts at: 2387 2409 2474 3181 3975 3995 2387
Size: 22 65 707 794 20 4057
Fragments arranged by size:
4057 794 707 65 22 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5665
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5630
DraI TTT'AAA
Cuts at: 2969 2969
Size: 5665
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4672 809
Size: 507 3356 1802
Fragments arranged by size:
3356 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2349 2373 3112
Size: 54 1425 50 275 18 519 24 739
Cuts at: 3112 4001 5630 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 739 519 275 54 50
43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 3112 1537
Size: 275 1300 4090
Fragments arranged by size:
4090 1300 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3595 1353
Size: 2242 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4252 4626 4772 387
Size: 1064 138 2663 374 146 1280
Fragments arranged by size:
2663 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3728 4325 5112 5380 1239
Size: 393 2096 597 787 268 1524
Fragments arranged by size:
2096 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2974 3352 2974
Size: 378 5287
FspI TGC'GCA
Cuts at: 4171 4171
Size: 5665
HaeII r_GCGC'y
Cuts at: 1678 4812 1678
Size: 3134 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4306 4338 4380 4412 1373
Size: 32 135 32 2734 32 42 32 2626
Fragments arranged by size:
2734 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3009 3255 669
Size: 988 281 1071 246 3079
Fragments arranged by size:
3079 1071 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5665
HpaI GTT'AAC
Cuts at: 3255 3255
Size: 5665
KpnI G_GTAC'C
Cuts at: 2990 2990
Size: 5665
MfeI C'AATT_G
Cuts at: 3264 3264
Size: 5665
MluI A'CGCG_T
Cuts at: 5541 5541
Size: 5665
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4779 4963 1305
Size: 3474 184 2007
Fragments arranged by size:
3474 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2351 2375 10
Size: 54 1425 343 519 24 3300
Fragments arranged by size:
3300 1425 519 343 54 24
MslI CAynn'nnrTG
Cuts at: 518 1166 2036 2514 2547 2624 2736 2738 3623
Size: 648 870 478 33 77 112 2 885
Cuts at: 3623 3982 4141 518
Size: 359 159 2042
Fragments arranged by size:
2042 885 870 648 478 359 159 112
77 33 2
MspA1I CmG'CkG
Cuts at: 1606 2454 2931 3081 3758 4906 5151 1606
Size: 848 477 150 677 1148 245 2120
Fragments arranged by size:
2120 1148 848 677 477 245 150
NcoI C'CATG_G
Cuts at: 513 1065 2352 513
Size: 552 1287 3826
Fragments arranged by size:
3826 1287 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5665
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5665
NotI GC'GGCC_GC
Cuts at: 3112 3112
Size: 5665
NspI r_CATG'y
Cuts at: 1132 1229 2030 2197 3023 4568 1132
Size: 97 801 167 826 1545 2229
Fragments arranged by size:
2229 1545 826 801 167 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5665
PciI A'CATG_T
Cuts at: 4564 4564
Size: 5665
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2768 1108
Size: 1660 4005
PmeI GTTT'AAAC
Cuts at: 2969 2969
Size: 5665
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3734 3766 5247 5279 3734
Size: 32 1481 32 4120
Fragments arranged by size:
4120 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5665
PsiI TTA'TAA
Cuts at: 3235 3235
Size: 5665
PstI C_TGCA'G
Cuts at: 830 2404 3017 830
Size: 1574 613 3478
Fragments arranged by size:
3478 1574 613
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5665
PvuII CAG'CTG
Cuts at: 2931 2931
Size: 5665
SacII CC_GC'GG
Cuts at: 2455 2455
Size: 5665
SalI G'TCGA_C
Cuts at: 1936 3007 1936
Size: 1071 4594
SbfI CC_TGCA'GG
Cuts at: 3017 3017
Size: 5665
ScaI AGT'ACT
Cuts at: 3913 5625 3913
Size: 1712 3953
SfcI C'TryA_G
Cuts at: 826 1045 2400 3013 4148 4829 5020 826
Size: 219 1355 613 1135 681 191 1471
Fragments arranged by size:
1471 1355 1135 681 613 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5665
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5665
SmaI CCC'GGG
Cuts at: 1872 2992 1872
Size: 1120 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3769 4670 4932 820
Size: 197 926 1826 901 262 1553
Fragments arranged by size:
1826 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3456 493
Size: 2963 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5665
SphI G_CATG'C
Cuts at: 1132 2197 3023 1132
Size: 1065 826 3774
Fragments arranged by size:
3774 1065 826
SspI AAT'ATT
Cuts at: 5 52 3589 5
Size: 47 3537 2081
Fragments arranged by size:
3537 2081 47
StuI AGG'CCT
Cuts at: 1517 2288 2711 1517
Size: 771 423 4471
Fragments arranged by size:
4471 771 423
StyI C'CwwG_G
Cuts at: 513 1065 2352 2706 3104 513
Size: 552 1287 354 398 3074
Fragments arranged by size:
3074 1287 552 398 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2400 2819 3692 3709 3862 4047 4492
Size: 1255 820 419 873 17 153 185 445
Cuts at: 4492 325
Size: 1498
Fragments arranged by size:
1498 1255 873 820 445 419 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2782 3911 5623
Size: 274 80 33 51 1443 805 1129 1712
Cuts at: 5623 96
Size: 138
Fragments arranged by size:
1712 1443 1129 805 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2709 2741 5625 5657 2709
Size: 32 2884 32 2717
Fragments arranged by size:
2884 2717 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5665
XbaI T'CTAG_A
Cuts at: 3001 3001
Size: 5665
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2078 2107 2344 2078
Size: 29 237 5399
Fragments arranged by size:
5399 237 29
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5665
XmaI C'CCGG_G
Cuts at: 1870 2990 1870
Size: 1120 4545
XmnI GAAnn'nnTTC
Cuts at: 3794 3794
Size: 5665
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3473 276
Size: 53 83 186 936 1939 2468
Fragments arranged by size:
2468 1939 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI
SexAI SfiI SfoI SrfI SwaI