Restriction map for
pFN21AB7132
(Circular) (Six-base) MAPSORT of: pFN21AB7132.seq Check: 339 from: 1 to: 5659
pFN21AB7132 5659 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2479 2561 2479
Size: 82 5577
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3469 278
Size: 53 83 186 936 1933 2468
Fragments arranged by size:
2468 1933 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3002 1937
Size: 1065 4594
Acc65I G'GTAC_C
Cuts at: 2980 2980
Size: 5659
AclI AA'CG_TT
Cuts at: 1563 3786 4159 1563
Size: 2223 373 3063
Fragments arranged by size:
3063 2223 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2036 2449 3722 5106 5374 1632
Size: 404 413 1273 1384 268 1917
Fragments arranged by size:
1917 1384 1273 413 404 268
AfeI AGC'GCT
Cuts at: 2030 2030
Size: 5659
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5462
AflIII A'CryG_T
Cuts at: 1203 4558 5535 1203
Size: 3355 977 1327
Fragments arranged by size:
3355 1327 977
AgeI A'CCGG_T
Cuts at: 4490 4490
Size: 5659
AhdI GACnn_n'nnGTC
Cuts at: 4388 4388
Size: 5659
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5659
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2133 1184
Size: 949 4710
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2309 2341 2309
Size: 32 5627
AlwNI CAG_nnn'CTG
Cuts at: 3402 4974 3402
Size: 1572 4087
ApaI G_GGCC'C
Cuts at: 2845 2845
Size: 5659
ApaLI G'TGCA_C
Cuts at: 3716 4872 3716
Size: 1156 4503
ApoI r'AATT_y
Cuts at: 1493 2968 3194 3346 5455 1493
Size: 1475 226 152 2109 1697
Fragments arranged by size:
2109 1697 1475 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4213 160
Size: 872 3181 1606
Fragments arranged by size:
3181 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
AvaI C'yCGr_G
Cuts at: 1870 1943 2984 1870
Size: 73 1041 4545
Fragments arranged by size:
4545 1041 73
BamHI G'GATC_C
Cuts at: 2989 3356 2989
Size: 367 5292
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2068 2204 2794 2980 4435
Size: 325 217 627 281 136 590 186 1455
Cuts at: 4435 618
Size: 1842
Fragments arranged by size:
1842 1455 627 590 325 281 217 186
136
BanII G_rGCy'C
Cuts at: 1391 2845 2978 1391
Size: 1454 133 4072
Fragments arranged by size:
4072 1454 133
BbeI G_GCGC'C
Cuts at: 2798 2798
Size: 5659
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4688
BbvCI CC'TCA_GC
Cuts at: 2040 2103 2040
Size: 63 5596
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3850 3884 1903
Size: 34 50 34 1829 34 3678
Fragments arranged by size:
3678 1829 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3551 4761 3551
Size: 1210 4449
BclI T'GATC_A
Cuts at: 1548 2139 2590 1548
Size: 591 451 4617
Fragments arranged by size:
4617 591 451
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4270 136
Size: 107 122 71 631 605 2598 1525
Fragments arranged by size:
2598 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5654 5654
Size: 5659
BlpI GC'TnA_GC
Cuts at: 3076 3076
Size: 5659
Bme1580I G_kGCm'C
Cuts at: 1790 2845 3720 4876 1790
Size: 1055 875 1156 2573
Fragments arranged by size:
2573 1156 1055 875
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5659
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3390 4348 442
Size: 416 53 83 399 24 1973 958 1753
Fragments arranged by size:
1973 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5659
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2224 2224
Size: 5659
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2336 2801 2894 4319 1239
Size: 1097 465 93 1425 2579
Fragments arranged by size:
2579 1425 1097 465 93
Bpu10I CC'TnA_GC
Cuts at: 2040 2103 2738 2040
Size: 63 635 4961
Fragments arranged by size:
4961 635 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2174 2441 3784 4649 4947 2174
Size: 267 1343 865 298 2886
Fragments arranged by size:
2886 1343 865 298 267
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4322 882
Size: 617 2823 2219
Fragments arranged by size:
2823 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3450 493
Size: 711 2246 2702
Fragments arranged by size:
2702 2246 711
BsaBI GATnn'nnATC
Cuts at: 5653 5653
Size: 5659
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2795 3466 3848 4483
Size: 53 83 186 936 1262 671 382 635
Cuts at: 4483 275
Size: 1451
Fragments arranged by size:
1451 1262 936 671 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4092 4490 4764 4911 5322 4092
Size: 398 274 147 411 4429
Fragments arranged by size:
4429 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2309 2339 1178
Size: 30 165 30 906 30 4498
Fragments arranged by size:
4498 906 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2116 2119 2120 2171 2200 2294 2681 2960
Size: 930 3 1 51 29 94 387 279
Cuts at: 2960 1186
Size: 3885
Fragments arranged by size:
3885 930 387 279 94 51 29 3
1
BseYI C'CCAG_C
Cuts at: 2305 2581 4862 5358 2305
Size: 276 2281 496 2606
Fragments arranged by size:
2606 2281 496 276
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2108 2786 1163
Size: 573 372 678 4036
Fragments arranged by size:
4036 678 573 372
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3109 3870 4898 1540
Size: 275 186 1108 761 1028 2301
Fragments arranged by size:
2301 1108 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2721 3720 3805 4876 2721
Size: 999 85 1071 3504
Fragments arranged by size:
3504 1071 999 85
BsmI GAATG_Cn'
Cuts at: 3170 3263 3170
Size: 93 5566
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2721 2845 2978 3720 3805 4876 1391
Size: 399 931 124 133 742 85 1071 2174
Fragments arranged by size:
2174 1071 931 742 399 133 124 85
BspEI T'CCGG_A
Cuts at: 5322 5322
Size: 5659
BspHI T'CATG_A
Cuts at: 3548 5278 3548
Size: 1730 3929
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2479 2561 3014 844
Size: 1635 82 453 3489
Fragments arranged by size:
3489 1635 453 82
BsrBI CCG'CTC
Cuts at: 1394 1823 3546 1394
Size: 429 1723 3507
Fragments arranged by size:
3507 1723 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2278 4154 4328 65
Size: 1183 1030 1876 174 1396
Fragments arranged by size:
1876 1396 1183 1030 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2398 2528 2609 4303 4490 1700
Size: 110 588 130 81 1694 187 2869
Fragments arranged by size:
2869 1694 588 187 130 110 81
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5659
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5659
BssSI C'ACGA_G
Cuts at: 3719 4731 3719
Size: 1012 4647
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5659
BstBI TT'CG_AA
Cuts at: 4463 4463
Size: 5659
BstEII G'GTnAC_C
Cuts at: 1184 2175 1184
Size: 991 4668
BstYI r'GATC_y
Cuts at: 1972 2989 3356 3741 3758 5210 5296 5308 5654
Size: 1017 367 385 17 1452 86 12 346
Cuts at: 5654 1972
Size: 1977
Fragments arranged by size:
1977 1452 1017 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2199 2199
Size: 5659
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5107
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4920
Fragments arranged by size:
4920 683 56
BtsI GCAGTG_nn'
Cuts at: 2809 3175 3969 3989 2809
Size: 366 794 20 4479
Fragments arranged by size:
4479 794 366 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5659
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5624
DraI TTT'AAA
Cuts at: 2963 2963
Size: 5659
DraIII CAC_nnn'GTG
Cuts at: 2241 2241
Size: 5659
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4666 809
Size: 507 3350 1802
Fragments arranged by size:
3350 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2530 3106 3995
Size: 54 1425 50 275 18 700 576 889
Cuts at: 3995 5624 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 700 576 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3106 1537
Size: 275 1294 4090
Fragments arranged by size:
4090 1294 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2015 2218 2271 2925 3589 1353
Size: 662 203 53 654 664 3423
Fragments arranged by size:
3423 664 662 654 203 53
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4246 4620 4766 387
Size: 1064 138 2657 374 146 1280
Fragments arranged by size:
2657 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2036 2336 2449 2801 2894 3722 4319
Size: 393 404 300 113 352 93 828 597
Cuts at: 4319 5106 5374 1239
Size: 787 268 1524
Fragments arranged by size:
1524 828 787 597 404 393 352 300
268 113 93
EcoNI CCTnn'n_nnAGG
Cuts at: 2156 2156
Size: 5659
EcoO109I rG'GnC_Cy
Cuts at: 2841 2842 2841
Size: 1 5658
EcoRI G'AATT_C
Cuts at: 2968 3346 2968
Size: 378 5281
EcoRV GAT'ATC
Cuts at: 2461 2461
Size: 5659
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2467 2467
Size: 5659
FspI TGC'GCA
Cuts at: 4165 4165
Size: 5659
HaeII r_GCGC'y
Cuts at: 1678 2032 2798 4806 1678
Size: 354 766 2008 2531
Fragments arranged by size:
2531 2008 766 354
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2159 2191 4300 4332 4374
Size: 32 135 32 587 32 2109 32 42
Cuts at: 4374 4406 1373
Size: 32 2626
Fragments arranged by size:
2626 2109 587 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3003 3249 669
Size: 988 281 1065 246 3079
Fragments arranged by size:
3079 1065 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5659
HpaI GTT'AAC
Cuts at: 3249 3249
Size: 5659
KasI G'GCGC_C
Cuts at: 2794 2794
Size: 5659
KpnI G_GTAC'C
Cuts at: 2984 2984
Size: 5659
MfeI C'AATT_G
Cuts at: 3258 3258
Size: 5659
MluI A'CGCG_T
Cuts at: 5535 5535
Size: 5659
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4773 4957 1305
Size: 3468 184 2007
Fragments arranged by size:
3468 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3837
Fragments arranged by size:
3837 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2489 2507 3617 3976 4135 518
Size: 648 1323 18 1110 359 159 2042
Fragments arranged by size:
2042 1323 1110 648 359 159 18
MspA1I CmG'CkG
Cuts at: 1606 2102 3075 3752 4900 5145 1606
Size: 496 973 677 1148 245 2120
Fragments arranged by size:
2120 1148 973 677 496 245
NaeI GCC'GGC
Cuts at: 2530 2530
Size: 5659
NarI GG'CG_CC
Cuts at: 2795 2795
Size: 5659
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5107
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5659
NgoMIV G'CCGG_C
Cuts at: 2528 2528
Size: 5659
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5659
NotI GC'GGCC_GC
Cuts at: 3106 3106
Size: 5659
NspI r_CATG'y
Cuts at: 1132 1229 2488 2882 3017 4562 1132
Size: 97 1259 394 135 1545 2229
Fragments arranged by size:
2229 1545 1259 394 135 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5659
PciI A'CATG_T
Cuts at: 4558 4558
Size: 5659
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5659
PfoI T'CCnGG_A
Cuts at: 2328 2871 2328
Size: 543 5116
PmeI GTTT'AAAC
Cuts at: 2963 2963
Size: 5659
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2309 2341 3728 3760 5241 5273 2309
Size: 32 1387 32 1481 32 2695
Fragments arranged by size:
2695 1481 1387 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5659
PsiI TTA'TAA
Cuts at: 3229 3229
Size: 5659
PspOMI G'GGCC_C
Cuts at: 2841 2841
Size: 5659
PstI C_TGCA'G
Cuts at: 830 3011 830
Size: 2181 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5659
PvuII CAG'CTG
Cuts at: 2102 2102
Size: 5659
SalI G'TCGA_C
Cuts at: 1936 3001 1936
Size: 1065 4594
SbfI CC_TGCA'GG
Cuts at: 3011 3011
Size: 5659
ScaI AGT'ACT
Cuts at: 3907 5619 3907
Size: 1712 3947
SfcI C'TryA_G
Cuts at: 826 1045 3007 4142 4823 5014 826
Size: 219 1962 1135 681 191 1471
Fragments arranged by size:
1962 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2796 2796
Size: 5659
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5659
SmaI CCC'GGG
Cuts at: 1872 2986 1872
Size: 1114 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2153 2420 3763 4664 4926 820
Size: 197 926 210 267 1343 901 262 1553
Fragments arranged by size:
1553 1343 926 901 267 262 210 197
SnaBI TAC'GTA
Cuts at: 493 3450 493
Size: 2957 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5659
SphI G_CATG'C
Cuts at: 1132 2488 3017 1132
Size: 1356 529 3774
Fragments arranged by size:
3774 1356 529
SspI AAT'ATT
Cuts at: 5 52 3583 5
Size: 47 3531 2081
Fragments arranged by size:
3531 2081 47
StuI AGG'CCT
Cuts at: 1517 2049 1517
Size: 532 5127
StyI C'CwwG_G
Cuts at: 513 1065 2059 2837 3098 513
Size: 552 994 778 261 3074
Fragments arranged by size:
3074 994 778 552 261
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2573 2752 2768 3686 3703 3856 4041
Size: 1255 993 179 16 918 17 153 185
Cuts at: 4041 4486 325
Size: 445 1498
Fragments arranged by size:
1498 1255 993 918 445 185 179 153
17 16
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3905 5617 96
Size: 274 80 33 51 1443 1928 1712 138
Fragments arranged by size:
1928 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2303 2335 5619 5651 2303
Size: 32 3284 32 2311
Fragments arranged by size:
3284 2311 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5659
XbaI T'CTAG_A
Cuts at: 2995 2995
Size: 5659
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5659
XmaI C'CCGG_G
Cuts at: 1870 2984 1870
Size: 1114 4545
XmnI GAAnn'nnTTC
Cuts at: 2049 3788 2049
Size: 1739 3920
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3467 276
Size: 53 83 186 936 1933 2468
Fragments arranged by size:
2468 1933 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I
EcoICRI FseI FspAI NruI NsiI PacI PmlI PpuMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SrfI SwaI XcmI