Restriction map for
pFN21AB7127
(Circular) (Six-base) MAPSORT of: pFN21AB7127.seq Check: 5855 from: 1 to: 5647
pFN21AB7127 5647 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2552 2552
Size: 5647
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3457 278
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2990 1937
Size: 1053 4594
Acc65I G'GTAC_C
Cuts at: 2968 2968
Size: 5647
AclI AA'CG_TT
Cuts at: 1563 3774 4147 1563
Size: 2211 373 3063
Fragments arranged by size:
3063 2211 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2061 2440 3710 5094 5362 1632
Size: 429 379 1270 1384 268 1917
Fragments arranged by size:
1917 1384 1270 429 379 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5450
AflIII A'CryG_T
Cuts at: 1203 4546 5523 1203
Size: 3343 977 1327
Fragments arranged by size:
3343 1327 977
AgeI A'CCGG_T
Cuts at: 4478 4478
Size: 5647
AhdI GACnn_n'nnGTC
Cuts at: 2308 4376 2308
Size: 2068 3579
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5647
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2112 2133 1184
Size: 928 21 4698
Fragments arranged by size:
4698 928 21
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2300 2332 2300
Size: 32 5615
AlwNI CAG_nnn'CTG
Cuts at: 3390 4962 3390
Size: 1572 4075
ApaI G_GGCC'C
Cuts at: 2836 2836
Size: 5647
ApaLI G'TGCA_C
Cuts at: 3704 4860 3704
Size: 1156 4491
ApoI r'AATT_y
Cuts at: 1493 2813 2956 3182 3334 5443 1493
Size: 1320 143 226 152 2109 1697
Fragments arranged by size:
2109 1697 1320 226 152 143
AseI AT'TA_AT
Cuts at: 160 1032 4201 160
Size: 872 3169 1606
Fragments arranged by size:
3169 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
AvaI C'yCGr_G
Cuts at: 1870 1943 2058 2972 1870
Size: 73 115 914 4545
Fragments arranged by size:
4545 914 115 73
BamHI G'GATC_C
Cuts at: 2725 2977 3344 2725
Size: 252 367 5028
Fragments arranged by size:
5028 367 252
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2161 2968 4423 618
Size: 325 217 627 374 807 1455 1842
Fragments arranged by size:
1842 1455 807 627 374 325 217
BanII G_rGCy'C
Cuts at: 1391 2836 2966 1391
Size: 1445 130 4072
Fragments arranged by size:
4072 1445 130
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2715 928
Size: 971 816 3860
Fragments arranged by size:
3860 971 816
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3838 3872 1903
Size: 34 50 34 1817 34 3678
Fragments arranged by size:
3678 1817 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3539 4749 3539
Size: 1210 4437
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5647
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4258 136
Size: 107 122 71 631 605 2586 1525
Fragments arranged by size:
2586 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5642 5642
Size: 5647
BlpI GC'TnA_GC
Cuts at: 3064 3064
Size: 5647
Bme1580I G_kGCm'C
Cuts at: 1790 2653 2836 3708 4864 1790
Size: 863 183 872 1156 2573
Fragments arranged by size:
2573 1156 872 863 183
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5647
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3378 4336 442
Size: 416 53 83 399 24 1961 958 1753
Fragments arranged by size:
1961 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5647
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2137 2137
Size: 5647
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2194 2248 2263 2327 2792 4307 1239
Size: 955 54 15 64 465 1515 2579
Fragments arranged by size:
2579 1515 955 465 64 54 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2033 2072 2354 3772 4637 4935 2033
Size: 39 282 1418 865 298 2745
Fragments arranged by size:
2745 1418 865 298 282 39
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4310 882
Size: 617 2811 2219
Fragments arranged by size:
2811 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3438 493
Size: 711 2234 2702
Fragments arranged by size:
2702 2234 711
BsaBI GATnn'nnATC
Cuts at: 5641 5641
Size: 5647
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3454 3836 4471 275
Size: 53 83 186 936 1921 382 635 1451
Fragments arranged by size:
1921 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4080 4478 4752 4899 5310 4080
Size: 398 274 147 411 4417
Fragments arranged by size:
4417 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2300 2330 1178
Size: 30 165 30 897 30 4495
Fragments arranged by size:
4495 897 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2035 2076 2113 2116 2120 2294 2357 2672
Size: 849 41 37 3 4 174 63 315
Cuts at: 2672 2687 1186
Size: 15 4146
Fragments arranged by size:
4146 849 315 174 63 41 37 15
4 3
BseYI C'CCAG_C
Cuts at: 2410 2572 4850 5346 2410
Size: 162 2278 496 2711
Fragments arranged by size:
2711 2278 496 162
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2777 2849 1163
Size: 573 1041 72 3961
Fragments arranged by size:
3961 1041 573 72
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3097 3858 4886 1540
Size: 275 186 1096 761 1028 2301
Fragments arranged by size:
2301 1096 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3708 3793 4864 3708
Size: 85 1071 4491
Fragments arranged by size:
4491 1071 85
BsmI GAATG_Cn'
Cuts at: 3158 3251 3158
Size: 93 5554
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2653 2836 2966 3708 3793 4864 1391
Size: 399 863 183 130 742 85 1071 2174
Fragments arranged by size:
2174 1071 863 742 399 183 130 85
BspEI T'CCGG_A
Cuts at: 5310 5310
Size: 5647
BspHI T'CATG_A
Cuts at: 3536 5266 3536
Size: 1730 3917
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2552 2773 3002 844
Size: 1708 221 229 3489
Fragments arranged by size:
3489 1708 229 221
BsrBI CCG'CTC
Cuts at: 1394 1823 3534 1394
Size: 429 1711 3507
Fragments arranged by size:
3507 1711 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4142 4316 65
Size: 1183 2894 174 1396
Fragments arranged by size:
2894 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2389 2778 4291 4478 1700
Size: 110 579 389 1513 187 2869
Fragments arranged by size:
2869 1513 579 389 187 110
BsrGI T'GTAC_A
Cuts at: 96 2529 96
Size: 2433 3214
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5647
BssSI C'ACGA_G
Cuts at: 3707 4719 3707
Size: 1012 4635
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5647
BstBI TT'CG_AA
Cuts at: 4451 4451
Size: 5647
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5647
BstXI CCAn_nnnn'nTGG
Cuts at: 2548 2548
Size: 5647
BstYI r'GATC_y
Cuts at: 1972 2182 2725 2977 3344 3729 3746 5198 5284
Size: 210 543 252 367 385 17 1452 86
Cuts at: 5284 5296 5642 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 543 385 367 346 252 210
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2199 2655 2199
Size: 456 5191
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5095
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4908
Fragments arranged by size:
4908 683 56
BtsI GCAGTG_nn'
Cuts at: 2029 2800 3163 3957 3977 2029
Size: 771 363 794 20 3699
Fragments arranged by size:
3699 794 771 363 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5647
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5612
DraI TTT'AAA
Cuts at: 2951 2951
Size: 5647
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4654 809
Size: 507 3338 1802
Fragments arranged by size:
3338 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2343 2521 2622
Size: 54 1425 50 275 18 513 178 101
Cuts at: 2622 3094 3983 5612 8
Size: 472 889 1629 43
Fragments arranged by size:
1629 1425 889 513 472 275 178 101
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3094 1537
Size: 275 1282 4090
Fragments arranged by size:
4090 1282 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2134 2271 2937 3577 1353
Size: 781 137 666 640 3423
Fragments arranged by size:
3423 781 666 640 137
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4234 4608 4754 387
Size: 1064 138 2645 374 146 1280
Fragments arranged by size:
2645 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2061 2194 2248 2263 2327 2440 2792
Size: 393 429 133 54 15 64 113 352
Cuts at: 2792 3710 4307 5094 5362 1239
Size: 918 597 787 268 1524
Fragments arranged by size:
1524 918 787 597 429 393 352 268
133 113 64 54 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2043 2156 2043
Size: 113 5534
EcoO109I rG'GnC_Cy
Cuts at: 2068 2785 2832 2833 2891 2068
Size: 717 47 1 58 4824
Fragments arranged by size:
4824 717 58 47 1
EcoRI G'AATT_C
Cuts at: 2813 2956 3334 2813
Size: 143 378 5126
Fragments arranged by size:
5126 378 143
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2066 2066
Size: 5647
FspI TGC'GCA
Cuts at: 2088 4153 2088
Size: 2065 3582
HaeII r_GCGC'y
Cuts at: 1678 4794 1678
Size: 3116 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2294 2326 4288 4320 4362
Size: 32 135 32 722 32 1962 32 42
Cuts at: 4362 4394 1373
Size: 32 2626
Fragments arranged by size:
2626 1962 722 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2991 3237 669
Size: 988 281 1053 246 3079
Fragments arranged by size:
3079 1053 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2463 748
Size: 1715 3932
HpaI GTT'AAC
Cuts at: 3237 3237
Size: 5647
KpnI G_GTAC'C
Cuts at: 2972 2972
Size: 5647
MfeI C'AATT_G
Cuts at: 3246 3246
Size: 5647
MluI A'CGCG_T
Cuts at: 5523 5523
Size: 5647
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2404 4761 4945 1305
Size: 1099 2357 184 2007
Fragments arranged by size:
2357 2007 1099 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2624 10
Size: 54 1425 343 792 3033
Fragments arranged by size:
3033 1425 792 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2225 3605 3964 4123 518
Size: 648 1059 1380 359 159 2042
Fragments arranged by size:
2042 1380 1059 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2482 3063 3740 4888 5133 1606
Size: 876 581 677 1148 245 2120
Fragments arranged by size:
2120 1148 876 677 581 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5095
NdeI CA'TA_TG
Cuts at: 387 2871 387
Size: 2484 3163
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5647
NotI GC'GGCC_GC
Cuts at: 3094 3094
Size: 5647
NspI r_CATG'y
Cuts at: 1132 1229 2808 3005 4550 1132
Size: 97 1579 197 1545 2229
Fragments arranged by size:
2229 1579 1545 197 97
PasI CC'CwG_GG
Cuts at: 1348 2071 2152 1348
Size: 723 81 4843
Fragments arranged by size:
4843 723 81
PciI A'CATG_T
Cuts at: 4546 4546
Size: 5647
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5647
PmeI GTTT'AAAC
Cuts at: 2951 2951
Size: 5647
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2300 2332 3716 3748 5229 5261 2300
Size: 32 1384 32 1481 32 2686
Fragments arranged by size:
2686 1481 1384 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2785 2891 2785
Size: 106 5541
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5647
PsiI TTA'TAA
Cuts at: 3217 3217
Size: 5647
PspOMI G'GGCC_C
Cuts at: 2832 2832
Size: 5647
PstI C_TGCA'G
Cuts at: 830 2999 830
Size: 2169 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5647
PvuII CAG'CTG
Cuts at: 2482 2482
Size: 5647
SalI G'TCGA_C
Cuts at: 1936 2989 1936
Size: 1053 4594
SbfI CC_TGCA'GG
Cuts at: 2999 2999
Size: 5647
ScaI AGT'ACT
Cuts at: 3895 5607 3895
Size: 1712 3935
SfcI C'TryA_G
Cuts at: 826 1045 2995 4130 4811 5002 826
Size: 219 1950 1135 681 191 1471
Fragments arranged by size:
1950 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5647
SmaI CCC'GGG
Cuts at: 1872 2974 1872
Size: 1102 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2012 2051 2369 3751 4652 4914
Size: 197 926 69 39 318 1382 901 262
Cuts at: 4914 820
Size: 1553
Fragments arranged by size:
1553 1382 926 901 318 262 197 69
39
SnaBI TAC'GTA
Cuts at: 493 3438 493
Size: 2945 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5647
SphI G_CATG'C
Cuts at: 1132 3005 1132
Size: 1873 3774
SspI AAT'ATT
Cuts at: 5 52 3571 5
Size: 47 3519 2081
Fragments arranged by size:
3519 2081 47
StuI AGG'CCT
Cuts at: 1517 2049 2604 1517
Size: 532 555 4560
Fragments arranged by size:
4560 555 532
StyI C'CwwG_G
Cuts at: 513 1065 2595 2828 3086 513
Size: 552 1530 233 258 3074
Fragments arranged by size:
3074 1530 552 258 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2072 2564 2759 3674 3691 3844 4029
Size: 1255 492 492 195 915 17 153 185
Cuts at: 4029 4474 325
Size: 445 1498
Fragments arranged by size:
1498 1255 915 492 492 445 195 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2529 3893 5605
Size: 274 80 33 51 1443 552 1364 1712
Cuts at: 5605 96
Size: 138
Fragments arranged by size:
1712 1443 1364 552 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5607 5639 5607
Size: 32 5615
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5647
XbaI T'CTAG_A
Cuts at: 2983 2983
Size: 5647
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5647
XmaI C'CCGG_G
Cuts at: 1870 2972 1870
Size: 1102 4545
XmnI GAAnn'nnTTC
Cuts at: 3776 3776
Size: 5647
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3455 276
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI