Restriction map for
pFN21AB7119
(Circular) (Six-base) MAPSORT of: pFN21AB7119.seq Check: 9267 from: 1 to: 5566
pFN21AB7119 5566 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2779 2779
Size: 5566
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3376 278
Size: 53 83 186 936 1840 2468
Fragments arranged by size:
2468 1840 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2763 2909 1937
Size: 826 146 4594
Fragments arranged by size:
4594 826 146
Acc65I G'GTAC_C
Cuts at: 2887 2887
Size: 5566
AclI AA'CG_TT
Cuts at: 1563 3693 4066 1563
Size: 2130 373 3063
Fragments arranged by size:
3063 2130 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3629 5013 5281 1632
Size: 1997 1384 268 1917
Fragments arranged by size:
1997 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5369
AflIII A'CryG_T
Cuts at: 1203 2541 2565 4465 5442 1203
Size: 1338 24 1900 977 1327
Fragments arranged by size:
1900 1338 1327 977 24
AgeI A'CCGG_T
Cuts at: 4397 4397
Size: 5566
AhdI GACnn_n'nnGTC
Cuts at: 4295 4295
Size: 5566
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5566
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5566
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2090 2122 2090
Size: 32 5534
AlwNI CAG_nnn'CTG
Cuts at: 2176 3309 4881 2176
Size: 1133 1572 2861
Fragments arranged by size:
2861 1572 1133
ApaLI G'TGCA_C
Cuts at: 3623 4779 3623
Size: 1156 4410
ApoI r'AATT_y
Cuts at: 1493 2875 3101 3253 5362 1493
Size: 1382 226 152 2109 1697
Fragments arranged by size:
2109 1697 1382 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4120 160
Size: 872 3088 1606
Fragments arranged by size:
3088 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5566
AvaI C'yCGr_G
Cuts at: 1870 1943 2289 2521 2891 1870
Size: 73 346 232 370 4545
Fragments arranged by size:
4545 370 346 232 73
BamHI G'GATC_C
Cuts at: 2896 3263 2896
Size: 367 5199
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2235 2887 4342 618
Size: 325 217 627 448 652 1455 1842
Fragments arranged by size:
1842 1455 652 627 448 325 217
BanII G_rGCy'C
Cuts at: 1391 2685 2745 2885 1391
Size: 1294 60 140 4072
Fragments arranged by size:
4072 1294 140 60
BbeI G_GCGC'C
Cuts at: 2239 2239
Size: 5566
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4595
BbvCI CC'TCA_GC
Cuts at: 2240 2346 2240
Size: 106 5460
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3757 3791 1903
Size: 34 1820 34 3678
Fragments arranged by size:
3678 1820 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3458 4668 3458
Size: 1210 4356
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5566
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2285 4177 136
Size: 107 122 71 631 605 613 1892 1525
Fragments arranged by size:
1892 1525 631 613 605 122 107 71
BglII A'GATC_T
Cuts at: 5561 5561
Size: 5566
BlpI GC'TnA_GC
Cuts at: 2333 2983 2333
Size: 650 4916
Bme1580I G_kGCm'C
Cuts at: 1790 3627 4783 1790
Size: 1837 1156 2573
Fragments arranged by size:
2573 1837 1156
BmgBI CAC'GTC
Cuts at: 1318 2188 2226 2566 2593 1318
Size: 870 38 340 27 4291
Fragments arranged by size:
4291 870 340 38 27
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3297 4255 442
Size: 416 53 83 399 24 1880 958 1753
Fragments arranged by size:
1880 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2021 1056
Size: 965 4601
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2101 2101
Size: 5566
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2147 2519 2675 4226 1239
Size: 908 372 156 1551 2579
Fragments arranged by size:
2579 1551 908 372 156
Bpu10I CC'TnA_GC
Cuts at: 2240 2346 2240
Size: 106 5460
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2846 3691 4556 4854 2846
Size: 845 865 298 3558
Fragments arranged by size:
3558 865 845 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4229 882
Size: 617 2730 2219
Fragments arranged by size:
2730 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3357 493
Size: 711 2153 2702
Fragments arranged by size:
2702 2153 711
BsaBI GATnn'nnATC
Cuts at: 2483 5560 2483
Size: 3077 2489
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2236 3373 3755 4390
Size: 53 83 186 936 703 1137 382 635
Cuts at: 4390 275
Size: 1451
Fragments arranged by size:
1451 1137 936 703 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3999 4397 4671 4818 5229 3999
Size: 398 274 147 411 4336
Fragments arranged by size:
4336 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2120 2150 2249 2279 1178
Size: 30 165 30 717 30 99 30 4465
Fragments arranged by size:
4465 717 165 99 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2828 1186
Size: 1642 3924
BseYI C'CCAG_C
Cuts at: 2197 2352 4769 5265 2197
Size: 155 2417 496 2498
Fragments arranged by size:
2498 2417 496 155
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2825 1163
Size: 573 1089 3904
Fragments arranged by size:
3904 1089 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2763 3016 3777 4805 1540
Size: 275 186 762 253 761 1028 2301
Fragments arranged by size:
2301 1028 762 761 275 253 186
BsiHKAI G_wGCw'C
Cuts at: 2685 2745 3627 3712 4783 2685
Size: 60 882 85 1071 3468
Fragments arranged by size:
3468 1071 882 85 60
BsmI GAATG_Cn'
Cuts at: 3077 3170 3077
Size: 93 5473
BsmBI CGTCTCn'nnnn_
Cuts at: 2105 2105
Size: 5566
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2685 2745 2885 3627 3712 4783 1391
Size: 399 895 60 140 742 85 1071 2174
Fragments arranged by size:
2174 1071 895 742 399 140 85 60
BspEI T'CCGG_A
Cuts at: 5229 5229
Size: 5566
BspHI T'CATG_A
Cuts at: 3455 5185 3455
Size: 1730 3836
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2779 2921 844
Size: 1935 142 3489
Fragments arranged by size:
3489 1935 142
BsrBI CCG'CTC
Cuts at: 1394 1823 3453 1394
Size: 429 1630 3507
Fragments arranged by size:
3507 1630 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4061 4235 65
Size: 1183 2813 174 1396
Fragments arranged by size:
2813 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2013 2771 4210 4397 1700
Size: 110 203 758 1439 187 2869
Fragments arranged by size:
2869 1439 758 203 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5566
BssHII G'CGCG_C
Cuts at: 1925 2317 2378 2425 2601 1925
Size: 392 61 47 176 4890
Fragments arranged by size:
4890 392 176 61 47
BssSI C'ACGA_G
Cuts at: 2093 2678 3626 4638 2093
Size: 585 948 1012 3021
Fragments arranged by size:
3021 1012 948 585
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5566
BstBI TT'CG_AA
Cuts at: 4370 4370
Size: 5566
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5566
BstYI r'GATC_y
Cuts at: 1972 2478 2896 3263 3648 3665 5117 5203 5215
Size: 506 418 367 385 17 1452 86 12
Cuts at: 5215 5561 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 506 418 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5014
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2254 487
Size: 683 56 1028 3799
Fragments arranged by size:
3799 1028 683 56
BtsI GCAGTG_nn'
Cuts at: 2305 2379 3082 3876 3896 2305
Size: 74 703 794 20 3975
Fragments arranged by size:
3975 794 703 74 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5566
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5531
DraI TTT'AAA
Cuts at: 2870 2870
Size: 5566
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4573 809
Size: 507 3257 1802
Fragments arranged by size:
3257 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2181 2586 2773
Size: 54 1425 50 275 18 351 405 187
Cuts at: 2773 3013 3902 5531 8
Size: 240 889 1629 43
Fragments arranged by size:
1629 1425 889 405 351 275 240 187
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3013 1537
Size: 275 1201 4090
Fragments arranged by size:
4090 1201 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2690 3496 1353
Size: 1337 806 3423
Fragments arranged by size:
3423 1337 806
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4153 4527 4673 387
Size: 1064 138 2564 374 146 1280
Fragments arranged by size:
2564 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2683 2743 2683
Size: 60 5506
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2147 2519 2675 3629 4226 5013 5281
Size: 393 515 372 156 954 597 787 268
Cuts at: 5281 1239
Size: 1524
Fragments arranged by size:
1524 954 787 597 515 393 372 268
156
EcoRI G'AATT_C
Cuts at: 2875 3253 2875
Size: 378 5188
FspI TGC'GCA
Cuts at: 4072 4072
Size: 5566
HaeII r_GCGC'y
Cuts at: 1678 2239 2494 4713 1678
Size: 561 255 2219 2531
Fragments arranged by size:
2531 2219 561 255
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2249 2281 4207 4239 4281
Size: 32 135 32 677 32 1926 32 42
Cuts at: 4281 4313 1373
Size: 32 2626
Fragments arranged by size:
2626 1926 677 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2764 2910 3156 669
Size: 988 281 826 146 246 3079
Fragments arranged by size:
3079 988 826 281 246 146
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5566
HpaI GTT'AAC
Cuts at: 3156 3156
Size: 5566
KasI G'GCGC_C
Cuts at: 2235 2235
Size: 5566
KpnI G_GTAC'C
Cuts at: 2891 2891
Size: 5566
MfeI C'AATT_G
Cuts at: 3165 3165
Size: 5566
MluI A'CGCG_T
Cuts at: 5442 5442
Size: 5566
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4680 4864 1305
Size: 3375 184 2007
Fragments arranged by size:
3375 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3744
Fragments arranged by size:
3744 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2228 3524 3883 4042 518
Size: 648 1062 1296 359 159 2042
Fragments arranged by size:
2042 1296 1062 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2173 2497 2982 3659 4807 5052 1606
Size: 567 324 485 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 567 485 324 245
NarI GG'CG_CC
Cuts at: 2236 2236
Size: 5566
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5014
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5566
NheI G'CTAG_C
Cuts at: 1052 2017 1052
Size: 965 4601
NotI GC'GGCC_GC
Cuts at: 3013 3013
Size: 5566
NspI r_CATG'y
Cuts at: 1132 1229 2545 2924 4469 1132
Size: 97 1316 379 1545 2229
Fragments arranged by size:
2229 1545 1316 379 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5566
PciI A'CATG_T
Cuts at: 2541 4465 2541
Size: 1924 3642
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2125 2176 1108
Size: 1017 51 4498
Fragments arranged by size:
4498 1017 51
PfoI T'CCnGG_A
Cuts at: 2124 2148 2124
Size: 24 5542
PmeI GTTT'AAAC
Cuts at: 2870 2870
Size: 5566
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2528 2560 3635 3667 5148 5180 2528
Size: 32 1075 32 1481 32 2914
Fragments arranged by size:
2914 1481 1075 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5566
PsiI TTA'TAA
Cuts at: 3136 3136
Size: 5566
PstI C_TGCA'G
Cuts at: 830 2918 830
Size: 2088 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5566
PvuII CAG'CTG
Cuts at: 2173 2497 2173
Size: 324 5242
SacI G_AGCT'C
Cuts at: 2685 2745 2685
Size: 60 5506
SalI G'TCGA_C
Cuts at: 1936 2762 2908 1936
Size: 826 146 4594
Fragments arranged by size:
4594 826 146
SapI GCTCTTCn'nnn_
Cuts at: 2690 2690
Size: 5566
SbfI CC_TGCA'GG
Cuts at: 2918 2918
Size: 5566
ScaI AGT'ACT
Cuts at: 3814 5526 3814
Size: 1712 3854
SfcI C'TryA_G
Cuts at: 826 1045 2914 4049 4730 4921 826
Size: 219 1869 1135 681 191 1471
Fragments arranged by size:
1869 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2285 2285
Size: 5566
SfoI GGC'GCC
Cuts at: 2237 2237
Size: 5566
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5566
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5566
SmaI CCC'GGG
Cuts at: 1872 2291 2893 1872
Size: 419 602 4545
Fragments arranged by size:
4545 602 419
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2861 3670 4571 4833 820
Size: 197 926 918 809 901 262 1553
Fragments arranged by size:
1553 926 918 901 809 262 197
SnaBI TAC'GTA
Cuts at: 493 3357 493
Size: 2864 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5566
SphI G_CATG'C
Cuts at: 1132 2924 1132
Size: 1792 3774
SspI AAT'ATT
Cuts at: 5 52 3490 5
Size: 47 3438 2081
Fragments arranged by size:
3438 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5566
StyI C'CwwG_G
Cuts at: 513 1065 2274 2781 3005 513
Size: 552 1209 507 224 3074
Fragments arranged by size:
3074 1209 552 507 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3593 3610 3763 3948 4393 325
Size: 1255 2013 17 153 185 445 1498
Fragments arranged by size:
2013 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2397 3812 5524
Size: 274 80 33 51 1443 420 1415 1712
Cuts at: 5524 96
Size: 138
Fragments arranged by size:
1712 1443 1415 420 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5526 5558 5526
Size: 32 5534
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5566
XbaI T'CTAG_A
Cuts at: 2902 2902
Size: 5566
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5566
XmaI C'CCGG_G
Cuts at: 1870 2289 2891 1870
Size: 419 602 4545
Fragments arranged by size:
4545 602 419
XmnI GAAnn'nnTTC
Cuts at: 3695 3695
Size: 5566
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3374 276
Size: 53 83 186 936 1840 2468
Fragments arranged by size:
2468 1840 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BfrBI BsiWI BstXI
BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacII SanDI SexAI SrfI
SwaI XcmI