Restriction map for
pFN21AB7118
(Circular) (Six-base) MAPSORT of: pFN21AB7118.seq Check: 955 from: 1 to: 5554
pFN21AB7118 5554 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3364 278
Size: 53 83 186 936 1828 2468
Fragments arranged by size:
2468 1828 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2897 1937
Size: 960 4594
Acc65I G'GTAC_C
Cuts at: 2875 2875
Size: 5554
AclI AA'CG_TT
Cuts at: 1563 3681 4054 1563
Size: 2118 373 3063
Fragments arranged by size:
3063 2118 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2114 2437 3617 5001 5269 1632
Size: 482 323 1180 1384 268 1917
Fragments arranged by size:
1917 1384 1180 482 323 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5357
AflIII A'CryG_T
Cuts at: 1203 4453 5430 1203
Size: 3250 977 1327
Fragments arranged by size:
3250 1327 977
AgeI A'CCGG_T
Cuts at: 4385 4385
Size: 5554
AhdI GACnn_n'nnGTC
Cuts at: 2672 4283 2672
Size: 1611 3943
AleI CACnn'nnGTG
Cuts at: 1166 2847 1166
Size: 1681 3873
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5554
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2540 2572 2540
Size: 32 5522
AlwNI CAG_nnn'CTG
Cuts at: 3297 4869 3297
Size: 1572 3982
ApaI G_GGCC'C
Cuts at: 2211 2234 2444 2211
Size: 23 210 5321
Fragments arranged by size:
5321 210 23
ApaLI G'TGCA_C
Cuts at: 3611 4767 3611
Size: 1156 4398
ApoI r'AATT_y
Cuts at: 1493 2863 3089 3241 5350 1493
Size: 1370 226 152 2109 1697
Fragments arranged by size:
2109 1697 1370 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4108 160
Size: 872 3076 1606
Fragments arranged by size:
3076 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5554
AvaI C'yCGr_G
Cuts at: 1870 1943 2158 2445 2489 2879 1870
Size: 73 215 287 44 390 4545
Fragments arranged by size:
4545 390 287 215 73 44
BamHI G'GATC_C
Cuts at: 2884 3251 2884
Size: 367 5187
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2875 4330 618
Size: 325 217 627 1088 1455 1842
Fragments arranged by size:
1842 1455 1088 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2144 2211 2234 2312 2444 2476 2873 1391
Size: 753 67 23 78 132 32 397 4072
Fragments arranged by size:
4072 753 397 132 78 67 32 23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4583
BbvCI CC'TCA_GC
Cuts at: 2189 2377 2189
Size: 188 5366
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3745 3779 1903
Size: 34 1808 34 3678
Fragments arranged by size:
3678 1808 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3446 4656 3446
Size: 1210 4344
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5554
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4165 136
Size: 107 122 71 631 605 2493 1525
Fragments arranged by size:
2493 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5549 5549
Size: 5554
BlpI GC'TnA_GC
Cuts at: 2971 2971
Size: 5554
Bme1580I G_kGCm'C
Cuts at: 1790 2091 2211 2234 2444 3615 4771 1790
Size: 301 120 23 210 1171 1156 2573
Fragments arranged by size:
2573 1171 1156 301 210 120 23
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5554
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2585 2672 2841
Size: 416 53 83 399 24 1168 87 169
Cuts at: 2841 3285 4243 442
Size: 444 958 1753
Fragments arranged by size:
1753 1168 958 444 416 399 169 87
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5554
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2728 2728
Size: 5554
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2129 2504 4214 1239
Size: 890 375 1710 2579
Fragments arranged by size:
2579 1710 890 375
Bpu10I CC'TnA_GC
Cuts at: 2189 2377 2713 2189
Size: 188 336 5030
Fragments arranged by size:
5030 336 188
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2537 2744 3679 4544 4842 2537
Size: 207 935 865 298 3249
Fragments arranged by size:
3249 935 865 298 207
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2775 4217 882
Size: 617 1276 1442 2219
Fragments arranged by size:
2219 1442 1276 617
BsaAI yAC'GTr
Cuts at: 493 1204 2287 2416 3345 493
Size: 711 1083 129 929 2702
Fragments arranged by size:
2702 1083 929 711 129
BsaBI GATnn'nnATC
Cuts at: 2333 5548 2333
Size: 3215 2339
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2076 3361 3743 4378
Size: 53 83 186 936 543 1285 382 635
Cuts at: 4378 275
Size: 1451
Fragments arranged by size:
1451 1285 936 635 543 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3987 4385 4659 4806 5217 3987
Size: 398 274 147 411 4324
Fragments arranged by size:
4324 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2693 2723 1178
Size: 30 165 30 1290 30 4009
Fragments arranged by size:
4009 1290 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2021 2143 2642 2717 1186
Size: 835 122 499 75 4023
Fragments arranged by size:
4023 835 499 122 75
BseYI C'CCAG_C
Cuts at: 2024 2211 4757 5253 2024
Size: 187 2546 496 2325
Fragments arranged by size:
2546 2325 496 187
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2556 1163
Size: 573 820 4161
Fragments arranged by size:
4161 820 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3004 3765 4793 1540
Size: 275 186 1003 761 1028 2301
Fragments arranged by size:
2301 1028 1003 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2714 3615 3700 4771 2714
Size: 901 85 1071 3497
Fragments arranged by size:
3497 1071 901 85
BsmI GAATG_Cn'
Cuts at: 3065 3158 3065
Size: 93 5461
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2144 2211 2234 2312 2444 2476
Size: 399 301 53 67 23 78 132 32
Cuts at: 2476 2714 2873 3615 3700 4771 1391
Size: 238 159 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 301 238 159 132
85 78 67 53 32 23
BspEI T'CCGG_A
Cuts at: 5217 5217
Size: 5554
BspHI T'CATG_A
Cuts at: 3443 5173 3443
Size: 1730 3824
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2909 844
Size: 2065 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2082 3441 1394
Size: 429 259 1359 3507
Fragments arranged by size:
3507 1359 429 259
BsrDI GCAATG_nn'
Cuts at: 65 1248 4049 4223 65
Size: 1183 2801 174 1396
Fragments arranged by size:
2801 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2741 4198 4385 1700
Size: 110 931 1457 187 2869
Fragments arranged by size:
2869 1457 931 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5554
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5554
BssSI C'ACGA_G
Cuts at: 3614 4626 3614
Size: 1012 4542
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5554
BstBI TT'CG_AA
Cuts at: 4358 4358
Size: 5554
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5554
BstXI CCAn_nnnn'nTGG
Cuts at: 2107 2203 2224 2254 2602 2669 2107
Size: 96 21 30 348 67 4992
Fragments arranged by size:
4992 348 96 67 30 21
BstYI r'GATC_y
Cuts at: 1972 2884 3251 3636 3653 5105 5191 5203 5549
Size: 912 367 385 17 1452 86 12 346
Cuts at: 5549 1972
Size: 1977
Fragments arranged by size:
1977 1452 912 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2346 513
Size: 552 939 342 3721
Fragments arranged by size:
3721 939 552 342
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4815
Fragments arranged by size:
4815 683 56
BtsI GCAGTG_nn'
Cuts at: 2842 3070 3864 3884 2842
Size: 228 794 20 4512
Fragments arranged by size:
4512 794 228 20
ClaI AT'CG_AT
Cuts at: 1554 2201 1554
Size: 647 4907
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5519
DraI TTT'AAA
Cuts at: 2858 2858
Size: 5554
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2657 2805 4561 809
Size: 507 1341 148 1756 1802
Fragments arranged by size:
1802 1756 1341 507 148
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2535 2739 3001
Size: 54 1425 50 275 18 705 204 262
Cuts at: 3001 3890 5519 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 705 275 262 204 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3001 1537
Size: 275 1189 4090
Fragments arranged by size:
4090 1189 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2619 3484 1353
Size: 1266 865 3423
Fragments arranged by size:
3423 1266 865
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2267 4141 4515 4661 387
Size: 1064 138 678 1874 374 146 1280
Fragments arranged by size:
1874 1280 1064 678 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2114 2129 2437 2504 3617 4214 5001
Size: 393 482 15 308 67 1113 597 787
Cuts at: 5001 5269 1239
Size: 268 1524
Fragments arranged by size:
1524 1113 787 597 482 393 308 268
67 15
EcoO109I rG'GnC_Cy
Cuts at: 2207 2208 2230 2440 2441 2461 2674 2719 2807
Size: 1 22 210 1 20 213 45 88
Cuts at: 2807 2207
Size: 4954
Fragments arranged by size:
4954 213 210 88 45 22 20 1
1
EcoRI G'AATT_C
Cuts at: 2863 3241 2863
Size: 378 5176
FspI TGC'GCA
Cuts at: 4060 4060
Size: 5554
HaeII r_GCGC'y
Cuts at: 1678 4701 1678
Size: 3023 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2066 2098 2129 2161 2658
Size: 32 135 32 494 32 31 32 497
Cuts at: 2658 2690 4195 4227 4269 4301 1373
Size: 32 1505 32 42 32 2626
Fragments arranged by size:
2626 1505 497 494 135 42 32 32
32 32 32 32 32 31
HincII GTy'rAC
Cuts at: 669 1657 1938 2898 3144 669
Size: 988 281 960 246 3079
Fragments arranged by size:
3079 988 960 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5554
HpaI GTT'AAC
Cuts at: 3144 3144
Size: 5554
KpnI G_GTAC'C
Cuts at: 2879 2879
Size: 5554
MfeI C'AATT_G
Cuts at: 3153 3153
Size: 5554
MluI A'CGCG_T
Cuts at: 5430 5430
Size: 5554
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4668 4852 1305
Size: 3363 184 2007
Fragments arranged by size:
3363 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3732
Fragments arranged by size:
3732 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2201 2847 3512 3871 4030 518
Size: 648 1035 646 665 359 159 2042
Fragments arranged by size:
2042 1035 665 648 646 359 159
MspA1I CmG'CkG
Cuts at: 1606 2348 2970 3647 4795 5040 1606
Size: 742 622 677 1148 245 2120
Fragments arranged by size:
2120 1148 742 677 622 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4063
Fragments arranged by size:
4063 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5554
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5554
NotI GC'GGCC_GC
Cuts at: 3001 3001
Size: 5554
NspI r_CATG'y
Cuts at: 1132 1229 2912 4457 1132
Size: 97 1683 1545 2229
Fragments arranged by size:
2229 1683 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2224 2794 1348
Size: 876 570 4108
Fragments arranged by size:
4108 876 570
PciI A'CATG_T
Cuts at: 4453 4453
Size: 5554
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5554
PfoI T'CCnGG_A
Cuts at: 2106 2253 2106
Size: 147 5407
PmeI GTTT'AAAC
Cuts at: 2858 2858
Size: 5554
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3623 3655 5136 5168 3623
Size: 32 1481 32 4009
Fragments arranged by size:
4009 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2461 2674 2719 2807 2461
Size: 213 45 88 5208
Fragments arranged by size:
5208 213 88 45
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5554
PsiI TTA'TAA
Cuts at: 3124 3124
Size: 5554
PspOMI G'GGCC_C
Cuts at: 2207 2230 2440 2207
Size: 23 210 5321
Fragments arranged by size:
5321 210 23
PstI C_TGCA'G
Cuts at: 830 2906 830
Size: 2076 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5554
SacII CC_GC'GG
Cuts at: 2349 2349
Size: 5554
SalI G'TCGA_C
Cuts at: 1936 2896 1936
Size: 960 4594
SanDI GG'GwC_CC
Cuts at: 2674 2807 2674
Size: 133 5421
SbfI CC_TGCA'GG
Cuts at: 2906 2906
Size: 5554
ScaI AGT'ACT
Cuts at: 3802 5514 3802
Size: 1712 3842
SfcI C'TryA_G
Cuts at: 826 1045 2902 4037 4718 4909 826
Size: 219 1857 1135 681 191 1471
Fragments arranged by size:
1857 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5554
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5554
SmaI CCC'GGG
Cuts at: 1872 2447 2881 1872
Size: 575 434 4545
Fragments arranged by size:
4545 575 434
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2552 2723 3658 4559 4821 820
Size: 197 926 609 171 935 901 262 1553
Fragments arranged by size:
1553 935 926 901 609 262 197 171
SnaBI TAC'GTA
Cuts at: 493 3345 493
Size: 2852 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5554
SphI G_CATG'C
Cuts at: 1132 2912 1132
Size: 1780 3774
SspI AAT'ATT
Cuts at: 5 52 3478 5
Size: 47 3426 2081
Fragments arranged by size:
3426 2081 47
StuI AGG'CCT
Cuts at: 1517 2375 1517
Size: 858 4696
StyI C'CwwG_G
Cuts at: 513 1065 2004 2752 2993 513
Size: 552 939 748 241 3074
Fragments arranged by size:
3074 939 748 552 241
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2558 3581 3598 3751 3936 4381 325
Size: 1255 978 1023 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1023 978 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3800 5512 96
Size: 274 80 33 51 1443 1823 1712 138
Fragments arranged by size:
1823 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2693 2725 5514 5546 2693
Size: 32 2789 32 2701
Fragments arranged by size:
2789 2701 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5554
XbaI T'CTAG_A
Cuts at: 2890 2890
Size: 5554
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2251 2572 2251
Size: 321 5233
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5554
XmaI C'CCGG_G
Cuts at: 1870 2445 2879 1870
Size: 575 434 4545
Fragments arranged by size:
4545 575 434
XmnI GAAnn'nnTTC
Cuts at: 3683 3683
Size: 5554
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3362 276
Size: 53 83 186 936 1828 2468
Fragments arranged by size:
2468 1828 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SacII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI PvuII RsrII SacI SapI
SexAI SfiI SfoI SrfI SwaI