Restriction map for
pFN21AB7117
(Circular) (Six-base) MAPSORT of: pFN21AB7117.seq Check: 7375 from: 1 to: 5554
pFN21AB7117 5554 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2514 3364 278
Size: 53 83 186 936 978 850 2468
Fragments arranged by size:
2468 978 936 850 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2514 2897 1937
Size: 577 383 4594
Fragments arranged by size:
4594 577 383
Acc65I G'GTAC_C
Cuts at: 2875 2875
Size: 5554
AclI AA'CG_TT
Cuts at: 1563 3681 4054 1563
Size: 2118 373 3063
Fragments arranged by size:
3063 2118 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2080 3617 5001 5269 1632
Size: 448 1537 1384 268 1917
Fragments arranged by size:
1917 1537 1384 448 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5357
AflIII A'CryG_T
Cuts at: 1203 4453 5430 1203
Size: 3250 977 1327
Fragments arranged by size:
3250 1327 977
AgeI A'CCGG_T
Cuts at: 4385 4385
Size: 5554
AhdI GACnn_n'nnGTC
Cuts at: 4283 4283
Size: 5554
AleI CACnn'nnGTG
Cuts at: 1166 2398 1166
Size: 1232 4322
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2073 1184
Size: 889 4665
AlwNI CAG_nnn'CTG
Cuts at: 3297 4869 3297
Size: 1572 3982
ApaLI G'TGCA_C
Cuts at: 3611 4767 3611
Size: 1156 4398
ApoI r'AATT_y
Cuts at: 1493 2863 3089 3241 5350 1493
Size: 1370 226 152 2109 1697
Fragments arranged by size:
2109 1697 1370 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4108 160
Size: 872 3076 1606
Fragments arranged by size:
3076 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5554
AvaI C'yCGr_G
Cuts at: 1870 1943 2701 2879 1870
Size: 73 758 178 4545
Fragments arranged by size:
4545 758 178 73
BamHI G'GATC_C
Cuts at: 2884 3251 2884
Size: 367 5187
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2807 2875 4330 618
Size: 325 217 627 1020 68 1455 1842
Fragments arranged by size:
1842 1455 1020 627 325 217 68
BanII G_rGCy'C
Cuts at: 1391 2668 2702 2873 1391
Size: 1277 34 171 4072
Fragments arranged by size:
4072 1277 171 34
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2440 2563 2572 928
Size: 971 541 123 9 3910
Fragments arranged by size:
3910 971 541 123 9
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3745 3779 1903
Size: 34 1808 34 3678
Fragments arranged by size:
3678 1808 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3446 4656 3446
Size: 1210 4344
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5554
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4165 136
Size: 107 122 71 631 605 2493 1525
Fragments arranged by size:
2493 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5549 5549
Size: 5554
BlpI GC'TnA_GC
Cuts at: 2971 2971
Size: 5554
Bme1580I G_kGCm'C
Cuts at: 1790 2659 3615 4771 1790
Size: 869 956 1156 2573
Fragments arranged by size:
2573 1156 956 869
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5554
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3285 4243 442
Size: 416 53 83 399 24 1868 958 1753
Fragments arranged by size:
1868 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2591 1056
Size: 1535 4019
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2093 2093
Size: 5554
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2375 4214 1239
Size: 917 219 1839 2579
Fragments arranged by size:
2579 1839 917 219
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2348 3679 4544 4842 2348
Size: 1331 865 298 3060
Fragments arranged by size:
3060 1331 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4217 882
Size: 617 2718 2219
Fragments arranged by size:
2718 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2287 3345 493
Size: 711 1083 1058 2702
Fragments arranged by size:
2702 1083 1058 711
BsaBI GATnn'nnATC
Cuts at: 5548 5548
Size: 5554
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2511 3361 3743 4378
Size: 53 83 186 936 978 850 382 635
Cuts at: 4378 275
Size: 1451
Fragments arranged by size:
1451 978 936 850 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3987 4385 4659 4806 5217 3987
Size: 398 274 147 411 4324
Fragments arranged by size:
4324 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5329
Fragments arranged by size:
5329 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2261 2372 1186
Size: 1075 111 4368
Fragments arranged by size:
4368 1075 111
BseYI C'CCAG_C
Cuts at: 2263 2667 2816 4757 5253 2263
Size: 404 149 1941 496 2564
Fragments arranged by size:
2564 1941 496 404 149
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4981
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2839 3004 3765 4793 1540
Size: 275 186 838 165 761 1028 2301
Fragments arranged by size:
2301 1028 838 761 275 186 165
BsiHKAI G_wGCw'C
Cuts at: 3615 3700 4771 3615
Size: 85 1071 4398
Fragments arranged by size:
4398 1071 85
BsmI GAATG_Cn'
Cuts at: 2050 3065 3158 2050
Size: 1015 93 4446
Fragments arranged by size:
4446 1015 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2659 2668 2702 2873 3615 3700 4771
Size: 399 869 9 34 171 742 85 1071
Cuts at: 4771 1391
Size: 2174
Fragments arranged by size:
2174 1071 869 742 399 171 85 34
9
BspEI T'CCGG_A
Cuts at: 5217 5217
Size: 5554
BspHI T'CATG_A
Cuts at: 3443 5173 3443
Size: 1730 3824
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2909 844
Size: 2065 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2676 3441 1394
Size: 429 853 765 3507
Fragments arranged by size:
3507 853 765 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4049 4223 65
Size: 1183 2801 174 1396
Fragments arranged by size:
2801 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2046 4198 4385 1700
Size: 110 236 2152 187 2869
Fragments arranged by size:
2869 2152 236 187 110
BsrGI T'GTAC_A
Cuts at: 96 2022 96
Size: 1926 3628
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5554
BssSI C'ACGA_G
Cuts at: 2273 3614 4626 2273
Size: 1341 1012 3201
Fragments arranged by size:
3201 1341 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5554
BstBI TT'CG_AA
Cuts at: 4358 4358
Size: 5554
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5554
BstXI CCAn_nnnn'nTGG
Cuts at: 2287 2533 2287
Size: 246 5308
BstYI r'GATC_y
Cuts at: 1972 2884 3251 3636 3653 5105 5191 5203 5549
Size: 912 367 385 17 1452 86 12 346
Cuts at: 5549 1972
Size: 1977
Fragments arranged by size:
1977 1452 912 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2335 2487 513
Size: 552 1270 152 3580
Fragments arranged by size:
3580 1270 552 152
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4815
Fragments arranged by size:
4815 683 56
BtsI GCAGTG_nn'
Cuts at: 3070 3864 3884 3070
Size: 794 20 4740
Fragments arranged by size:
4740 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5554
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5519
DraI TTT'AAA
Cuts at: 2858 2858
Size: 5554
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4561 809
Size: 507 3245 1802
Fragments arranged by size:
3245 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2065 2277 2622
Size: 54 1425 50 275 18 235 212 345
Cuts at: 2622 2710 3001 3890 5519 8
Size: 88 291 889 1629 43
Fragments arranged by size:
1629 1425 889 345 291 275 235 212
88 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3001 1537
Size: 275 1189 4090
Fragments arranged by size:
4090 1189 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2360 3484 1353
Size: 1007 1124 3423
Fragments arranged by size:
3423 1124 1007
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4141 4515 4661 387
Size: 1064 138 2552 374 146 1280
Fragments arranged by size:
2552 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2080 2156 2375 3617 4214 5001 5269
Size: 393 448 76 219 1242 597 787 268
Cuts at: 5269 1239
Size: 1524
Fragments arranged by size:
1524 1242 787 597 448 393 268 219
76
EcoNI CCTnn'n_nnAGG
Cuts at: 2357 2357
Size: 5554
EcoO109I rG'GnC_Cy
Cuts at: 2341 2341
Size: 5554
EcoRI G'AATT_C
Cuts at: 2863 3241 2863
Size: 378 5176
FspI TGC'GCA
Cuts at: 4060 4060
Size: 5554
HaeII r_GCGC'y
Cuts at: 1678 2674 4701 1678
Size: 996 2027 2531
Fragments arranged by size:
2531 2027 996
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2132 2164 2828 2860 4195
Size: 32 135 32 560 32 664 32 1335
Cuts at: 4195 4227 4269 4301 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1335 664 560 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2898 3144 669
Size: 988 281 960 246 3079
Fragments arranged by size:
3079 988 960 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5554
HpaI GTT'AAC
Cuts at: 3144 3144
Size: 5554
KpnI G_GTAC'C
Cuts at: 2879 2879
Size: 5554
MfeI C'AATT_G
Cuts at: 3153 3153
Size: 5554
MluI A'CGCG_T
Cuts at: 5430 5430
Size: 5554
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4668 4852 1305
Size: 3363 184 2007
Fragments arranged by size:
3363 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2279 10
Size: 54 1425 343 447 3285
Fragments arranged by size:
3285 1425 447 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2285 2398 2417 3512 3871 4030 518
Size: 648 1119 113 19 1095 359 159 2042
Fragments arranged by size:
2042 1119 1095 648 359 159 113 19
MspA1I CmG'CkG
Cuts at: 1606 2841 2970 3647 4795 5040 1606
Size: 1235 129 677 1148 245 2120
Fragments arranged by size:
2120 1235 1148 677 245 129
NcoI C'CATG_G
Cuts at: 513 1065 2335 2487 513
Size: 552 1270 152 3580
Fragments arranged by size:
3580 1270 552 152
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5554
NheI G'CTAG_C
Cuts at: 1052 2587 1052
Size: 1535 4019
NotI GC'GGCC_GC
Cuts at: 3001 3001
Size: 5554
NspI r_CATG'y
Cuts at: 1132 1229 2912 4457 1132
Size: 97 1683 1545 2229
Fragments arranged by size:
2229 1683 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5554
PciI A'CATG_T
Cuts at: 4453 4453
Size: 5554
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 4652
PmeI GTTT'AAAC
Cuts at: 2858 2858
Size: 5554
PmlI CAC'GTG
Cuts at: 2287 2287
Size: 5554
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3623 3655 5136 5168 3623
Size: 32 1481 32 4009
Fragments arranged by size:
4009 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2341 2341
Size: 5554
PshAI GACnn'nnGTC
Cuts at: 1613 2408 1613
Size: 795 4759
PsiI TTA'TAA
Cuts at: 3124 3124
Size: 5554
PstI C_TGCA'G
Cuts at: 830 2125 2244 2906 830
Size: 1295 119 662 3478
Fragments arranged by size:
3478 1295 662 119
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5554
SalI G'TCGA_C
Cuts at: 1936 2896 1936
Size: 960 4594
SanDI GG'GwC_CC
Cuts at: 2341 2341
Size: 5554
SbfI CC_TGCA'GG
Cuts at: 2906 2906
Size: 5554
ScaI AGT'ACT
Cuts at: 3802 5514 3802
Size: 1712 3842
SfcI C'TryA_G
Cuts at: 826 1045 2121 2240 2902 4037 4718 4909 826
Size: 219 1076 119 662 1135 681 191 1471
Fragments arranged by size:
1471 1135 1076 681 662 219 191 119
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5554
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5554
SmaI CCC'GGG
Cuts at: 1872 2881 1872
Size: 1009 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2327 3658 4559 4821 820
Size: 197 926 384 1331 901 262 1553
Fragments arranged by size:
1553 1331 926 901 384 262 197
SnaBI TAC'GTA
Cuts at: 493 3345 493
Size: 2852 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5554
SphI G_CATG'C
Cuts at: 1132 2912 1132
Size: 1780 3774
SspI AAT'ATT
Cuts at: 5 52 3478 5
Size: 47 3426 2081
Fragments arranged by size:
3426 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5554
StyI C'CwwG_G
Cuts at: 513 1065 2335 2487 2993 513
Size: 552 1270 152 506 3074
Fragments arranged by size:
3074 1270 552 506 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2071 3581 3598 3751 3936 4381 325
Size: 1255 491 1510 17 153 185 445 1498
Fragments arranged by size:
1510 1498 1255 491 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2022 3800 5512
Size: 274 80 33 51 1443 45 1778 1712
Cuts at: 5512 96
Size: 138
Fragments arranged by size:
1778 1712 1443 274 138 80 51 45
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5514 5546 5514
Size: 32 5522
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5554
XbaI T'CTAG_A
Cuts at: 2890 2890
Size: 5554
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2494 2494
Size: 5554
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5554
XmaI C'CCGG_G
Cuts at: 1870 2879 1870
Size: 1009 4545
XmnI GAAnn'nnTTC
Cuts at: 3683 3683
Size: 5554
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2512 3362 276
Size: 53 83 186 936 978 850 2468
Fragments arranged by size:
2468 978 936 850 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SapI SexAI SfiI SfoI
SrfI SwaI