Restriction map for
pFN21AB7116
(Circular) (Six-base) MAPSORT of: pFN21AB7116.seq Check: 3531 from: 1 to: 5545
pFN21AB7116 5545 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3355 278
Size: 53 83 186 936 1819 2468
Fragments arranged by size:
2468 1819 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2888 1937
Size: 951 4594
Acc65I G'GTAC_C
Cuts at: 2866 2866
Size: 5545
AclI AA'CG_TT
Cuts at: 1563 3672 4045 1563
Size: 2109 373 3063
Fragments arranged by size:
3063 2109 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3608 4992 5260 1632
Size: 1976 1384 268 1917
Fragments arranged by size:
1976 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5348
AflIII A'CryG_T
Cuts at: 1203 4444 5421 1203
Size: 3241 977 1327
Fragments arranged by size:
3241 1327 977
AgeI A'CCGG_T
Cuts at: 4376 4376
Size: 5545
AhdI GACnn_n'nnGTC
Cuts at: 4274 4274
Size: 5545
AleI CACnn'nnGTG
Cuts at: 1166 2041 1166
Size: 875 4670
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5545
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2690 2722 2690
Size: 32 5513
AlwNI CAG_nnn'CTG
Cuts at: 3288 4860 3288
Size: 1572 3973
ApaLI G'TGCA_C
Cuts at: 2253 3602 4758 2253
Size: 1349 1156 3040
Fragments arranged by size:
3040 1349 1156
ApoI r'AATT_y
Cuts at: 1493 2522 2854 3080 3232 5341 1493
Size: 1029 332 226 152 2109 1697
Fragments arranged by size:
2109 1697 1029 332 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4099 160
Size: 872 3067 1606
Fragments arranged by size:
3067 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5545
AvaI C'yCGr_G
Cuts at: 1870 1943 2499 2870 1870
Size: 73 556 371 4545
Fragments arranged by size:
4545 556 371 73
BamHI G'GATC_C
Cuts at: 2875 3242 2875
Size: 367 5178
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2465 2740 2866 4321 618
Size: 325 217 627 678 275 126 1455 1842
Fragments arranged by size:
1842 1455 678 627 325 275 217 126
BanII G_rGCy'C
Cuts at: 1391 2446 2500 2552 2864 1391
Size: 1055 54 52 312 4072
Fragments arranged by size:
4072 1055 312 54 52
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2480 928
Size: 971 581 3993
Fragments arranged by size:
3993 971 581
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3736 3770 1903
Size: 34 1799 34 3678
Fragments arranged by size:
3678 1799 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2355 2447 3437 4647 2355
Size: 92 990 1210 3253
Fragments arranged by size:
3253 1210 990 92
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5545
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2411 2749 4156
Size: 107 122 71 631 605 739 338 1407
Cuts at: 4156 136
Size: 1525
Fragments arranged by size:
1525 1407 739 631 605 338 122 107
71
BglII A'GATC_T
Cuts at: 5540 5540
Size: 5545
BlpI GC'TnA_GC
Cuts at: 2962 2962
Size: 5545
Bme1580I G_kGCm'C
Cuts at: 1790 2257 2468 2745 3606 4762 1790
Size: 467 211 277 861 1156 2573
Fragments arranged by size:
2573 1156 861 467 277 211
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5545
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2328 3276 4234
Size: 416 53 83 399 24 911 948 958
Cuts at: 4234 442
Size: 1753
Fragments arranged by size:
1753 958 948 911 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5545
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2190 2399 2591 4205 1239
Size: 951 209 192 1614 2579
Fragments arranged by size:
2579 1614 951 209 192
Bpu10I CC'TnA_GC
Cuts at: 2295 2295
Size: 5545
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3670 4535 4833 3670
Size: 865 298 4382
Fragments arranged by size:
4382 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4208 882
Size: 617 2709 2219
Fragments arranged by size:
2709 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3336 493
Size: 711 2132 2702
Fragments arranged by size:
2702 2132 711
BsaBI GATnn'nnATC
Cuts at: 5539 5539
Size: 5545
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3352 3734 4369 275
Size: 53 83 186 936 1819 382 635 1451
Fragments arranged by size:
1819 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3978 4376 4650 4797 5208 3978
Size: 398 274 147 411 4315
Fragments arranged by size:
4315 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2690 2720 1178
Size: 30 165 30 1287 30 4003
Fragments arranged by size:
4003 1287 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2396 2399 1186
Size: 1210 3 4332
Fragments arranged by size:
4332 1210 3
BseYI C'CCAG_C
Cuts at: 2809 4748 5244 2809
Size: 1939 496 3110
Fragments arranged by size:
3110 1939 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2806 1163
Size: 573 1070 3902
Fragments arranged by size:
3902 1070 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2995 3756 4784 1540
Size: 275 186 994 761 1028 2301
Fragments arranged by size:
2301 1028 994 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2257 2500 3606 3691 4762 2257
Size: 243 1106 85 1071 3040
Fragments arranged by size:
3040 1106 1071 243 85
BsmI GAATG_Cn'
Cuts at: 3056 3149 3056
Size: 93 5452
BsmBI CGTCTCn'nnnn_
Cuts at: 2453 2453
Size: 5545
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2257 2446 2468 2500 2552 2745 2864
Size: 399 467 189 22 32 52 193 119
Cuts at: 2864 3606 3691 4762 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 467 399 193 189 119
85 52 32 22
BspEI T'CCGG_A
Cuts at: 5208 5208
Size: 5545
BspHI T'CATG_A
Cuts at: 3434 5164 3434
Size: 1730 3815
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2900 844
Size: 2056 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3432 1394
Size: 429 1609 3507
Fragments arranged by size:
3507 1609 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4040 4214 65
Size: 1183 2792 174 1396
Fragments arranged by size:
2792 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4189 4376 1700
Size: 110 2379 187 2869
Fragments arranged by size:
2869 2379 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5545
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5545
BssSI C'ACGA_G
Cuts at: 3605 4617 3605
Size: 1012 4533
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5545
BstBI TT'CG_AA
Cuts at: 4349 4349
Size: 5545
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5545
BstYI r'GATC_y
Cuts at: 1972 2289 2875 3242 3627 3644 5096 5182 5194
Size: 317 586 367 385 17 1452 86 12
Cuts at: 5194 5540 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 586 385 367 346 317 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2511 513
Size: 552 939 507 3547
Fragments arranged by size:
3547 939 552 507
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4806
Fragments arranged by size:
4806 683 56
BtsI GCAGTG_nn'
Cuts at: 2420 2716 3061 3855 3875 2420
Size: 296 345 794 20 4090
Fragments arranged by size:
4090 794 345 296 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5545
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5510
DraI TTT'AAA
Cuts at: 2849 2849
Size: 5545
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4552 809
Size: 507 3236 1802
Fragments arranged by size:
3236 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2651 2685
Size: 54 1425 50 275 18 177 644 34
Cuts at: 2685 2992 3881 5510 8
Size: 307 889 1629 43
Fragments arranged by size:
1629 1425 889 644 307 275 177 54
50 43 34 18
EagI C'GGCC_G
Cuts at: 1537 1812 2992 1537
Size: 275 1180 4090
Fragments arranged by size:
4090 1180 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2335 2557 3475 1353
Size: 982 222 918 3423
Fragments arranged by size:
3423 982 918 222
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2116 2190 4132 4506 4652 387
Size: 1064 138 527 74 1942 374 146 1280
Fragments arranged by size:
1942 1280 1064 527 374 146 138 74
EcoICRI GAG'CTC
Cuts at: 2498 2498
Size: 5545
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2190 2399 2591 3608 4205 4992 5260
Size: 393 558 209 192 1017 597 787 268
Cuts at: 5260 1239
Size: 1524
Fragments arranged by size:
1524 1017 787 597 558 393 268 209
192
EcoNI CCTnn'n_nnAGG
Cuts at: 2381 2381
Size: 5545
EcoRI G'AATT_C
Cuts at: 2854 3232 2854
Size: 378 5167
FspI TGC'GCA
Cuts at: 4051 4051
Size: 5545
HaeII r_GCGC'y
Cuts at: 1678 4692 1678
Size: 3014 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2564 2596 4186 4218 4260
Size: 32 135 32 992 32 1590 32 42
Cuts at: 4260 4292 1373
Size: 32 2626
Fragments arranged by size:
2626 1590 992 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2071 2889 3135 669
Size: 988 281 133 818 246 3079
Fragments arranged by size:
3079 988 818 281 246 133
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5545
HpaI GTT'AAC
Cuts at: 3135 3135
Size: 5545
KpnI G_GTAC'C
Cuts at: 2870 2870
Size: 5545
MfeI C'AATT_G
Cuts at: 3144 3144
Size: 5545
MluI A'CGCG_T
Cuts at: 5421 5421
Size: 5545
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4659 4843 1305
Size: 3354 184 2007
Fragments arranged by size:
3354 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 3546
Fragments arranged by size:
3546 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2041 3503 3862 4021 518
Size: 648 875 1462 359 159 2042
Fragments arranged by size:
2042 1462 875 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2961 3638 4786 5031 1606
Size: 1355 677 1148 245 2120
Fragments arranged by size:
2120 1355 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4054
Fragments arranged by size:
4054 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5545
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5545
NotI GC'GGCC_GC
Cuts at: 2992 2992
Size: 5545
NspI r_CATG'y
Cuts at: 1132 1229 2903 4448 1132
Size: 97 1674 1545 2229
Fragments arranged by size:
2229 1674 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2164 1348
Size: 816 4729
PciI A'CATG_T
Cuts at: 4444 4444
Size: 5545
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2170 1108
Size: 1062 4483
PmeI GTTT'AAAC
Cuts at: 2849 2849
Size: 5545
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2690 2722 3614 3646 5127 5159 2690
Size: 32 892 32 1481 32 3076
Fragments arranged by size:
3076 1481 892 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5545
PsiI TTA'TAA
Cuts at: 3115 3115
Size: 5545
PspXI vC'TCGA_Gb
Cuts at: 2499 2499
Size: 5545
PstI C_TGCA'G
Cuts at: 830 2157 2304 2723 2897 830
Size: 1327 147 419 174 3478
Fragments arranged by size:
3478 1327 419 174 147
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5545
SacI G_AGCT'C
Cuts at: 2500 2500
Size: 5545
SalI G'TCGA_C
Cuts at: 1936 2887 1936
Size: 951 4594
SapI GCTCTTCn'nnn_
Cuts at: 2557 2557
Size: 5545
SbfI CC_TGCA'GG
Cuts at: 2897 2897
Size: 5545
ScaI AGT'ACT
Cuts at: 3793 5505 3793
Size: 1712 3833
SfcI C'TryA_G
Cuts at: 826 1045 2153 2300 2719 2893 4028 4709 4900
Size: 219 1108 147 419 174 1135 681 191
Cuts at: 4900 826
Size: 1471
Fragments arranged by size:
1471 1135 1108 681 419 219 191 174
147
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5545
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5545
SmaI CCC'GGG
Cuts at: 1872 2872 1872
Size: 1000 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2499 3649 4550 4812 820
Size: 197 926 556 1150 901 262 1553
Fragments arranged by size:
1553 1150 926 901 556 262 197
SnaBI TAC'GTA
Cuts at: 493 3336 493
Size: 2843 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5545
SphI G_CATG'C
Cuts at: 1132 2903 1132
Size: 1771 3774
SspI AAT'ATT
Cuts at: 5 52 3469 5
Size: 47 3417 2081
Fragments arranged by size:
3417 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5545
StyI C'CwwG_G
Cuts at: 513 1065 2004 2130 2594 2984 513
Size: 552 939 126 464 390 3074
Fragments arranged by size:
3074 939 552 464 390 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2161 2272 3572 3589 3742 3927 4372
Size: 1255 581 111 1300 17 153 185 445
Cuts at: 4372 325
Size: 1498
Fragments arranged by size:
1498 1300 1255 581 445 185 153 111
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3791 5503 96
Size: 274 80 33 51 1443 1814 1712 138
Fragments arranged by size:
1814 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5505 5537 5505
Size: 32 5513
Tth111I GACn'n_nGTC
Cuts at: 1316 2148 1316
Size: 832 4713
XbaI T'CTAG_A
Cuts at: 2881 2881
Size: 5545
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2680 2680
Size: 5545
XhoI C'TCGA_G
Cuts at: 1943 2499 1943
Size: 556 4989
XmaI C'CCGG_G
Cuts at: 1870 2870 1870
Size: 1000 4545
XmnI GAAnn'nnTTC
Cuts at: 3674 3674
Size: 5545
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3353 276
Size: 53 83 186 936 1819 2468
Fragments arranged by size:
2468 1819 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI
PvuI SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI
PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI