Restriction map for
pFN21AB7114
(Circular) (Six-base) MAPSORT of: pFN21AB7114.seq Check: 6714 from: 1 to: 5524
pFN21AB7114 5524 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3334 278
Size: 53 83 186 936 1798 2468
Fragments arranged by size:
2468 1798 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2867 1937
Size: 930 4594
Acc65I G'GTAC_C
Cuts at: 2702 2845 2702
Size: 143 5381
AclI AA'CG_TT
Cuts at: 1563 3651 4024 1563
Size: 2088 373 3063
Fragments arranged by size:
3063 2088 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3587 4971 5239 1632
Size: 1955 1384 268 1917
Fragments arranged by size:
1955 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5327
AflIII A'CryG_T
Cuts at: 1203 4423 5400 1203
Size: 3220 977 1327
Fragments arranged by size:
3220 1327 977
AgeI A'CCGG_T
Cuts at: 4355 4355
Size: 5524
AhdI GACnn_n'nnGTC
Cuts at: 4253 4253
Size: 5524
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5524
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5524
AlwNI CAG_nnn'CTG
Cuts at: 3267 4839 3267
Size: 1572 3952
ApaI G_GGCC'C
Cuts at: 2239 2239
Size: 5524
ApaLI G'TGCA_C
Cuts at: 2680 3581 4737 2680
Size: 901 1156 3467
Fragments arranged by size:
3467 1156 901
ApoI r'AATT_y
Cuts at: 1493 2833 3059 3211 5320 1493
Size: 1340 226 152 2109 1697
Fragments arranged by size:
2109 1697 1340 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4078 160
Size: 872 3046 1606
Fragments arranged by size:
3046 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5524
AvaI C'yCGr_G
Cuts at: 1870 1943 2796 2849 1870
Size: 73 853 53 4545
Fragments arranged by size:
4545 853 73 53
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1999 2032 1999
Size: 33 5491
BamHI G'GATC_C
Cuts at: 2854 3221 2854
Size: 367 5157
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2316 2346 2702 2845 4300
Size: 325 217 627 529 30 356 143 1455
Cuts at: 4300 618
Size: 1842
Fragments arranged by size:
1842 1455 627 529 356 325 217 143
30
BanII G_rGCy'C
Cuts at: 1391 2239 2385 2619 2762 2843 1391
Size: 848 146 234 143 81 4072
Fragments arranged by size:
4072 848 234 146 143 81
BbeI G_GCGC'C
Cuts at: 2350 2350
Size: 5524
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2080 928
Size: 971 181 4372
Fragments arranged by size:
4372 971 181
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3715 3749 1903
Size: 34 1778 34 3678
Fragments arranged by size:
3678 1778 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3416 4626 3416
Size: 1210 4314
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5524
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2178 4135 136
Size: 107 122 71 631 605 506 1957 1525
Fragments arranged by size:
1957 1525 631 605 506 122 107 71
BglII A'GATC_T
Cuts at: 5519 5519
Size: 5524
BlpI GC'TnA_GC
Cuts at: 2941 2941
Size: 5524
Bme1580I G_kGCm'C
Cuts at: 1790 2239 2684 3585 4741 1790
Size: 449 445 901 1156 2573
Fragments arranged by size:
2573 1156 901 449 445
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5524
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3255 4213 442
Size: 416 53 83 399 24 1838 958 1753
Fragments arranged by size:
1838 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5524
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2032 4184 1239
Size: 793 2152 2579
Fragments arranged by size:
2579 2152 793
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2655 3649 4514 4812 2655
Size: 994 865 298 3367
Fragments arranged by size:
3367 994 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2793 4187 882
Size: 617 1294 1394 2219
Fragments arranged by size:
2219 1394 1294 617
BsaAI yAC'GTr
Cuts at: 493 1204 2287 3315 493
Size: 711 1083 1028 2702
Fragments arranged by size:
2702 1083 1028 711
BsaBI GATnn'nnATC
Cuts at: 5518 5518
Size: 5524
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2347 3331 3713 4348
Size: 53 83 186 936 814 984 382 635
Cuts at: 4348 275
Size: 1451
Fragments arranged by size:
1451 984 936 814 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3957 4355 4629 4776 5187 3957
Size: 398 274 147 411 4294
Fragments arranged by size:
4294 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2036 2066 1178
Size: 30 165 30 633 30 4636
Fragments arranged by size:
4636 633 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2766 1186
Size: 1580 3944
BseYI C'CCAG_C
Cuts at: 4727 5223 4727
Size: 496 5028
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4951
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2124 2974 3735 4763 1540
Size: 275 186 123 850 761 1028 2301
Fragments arranged by size:
2301 1028 850 761 275 186 123
BsiHKAI G_wGCw'C
Cuts at: 2619 2684 3585 3670 4741 2619
Size: 65 901 85 1071 3402
Fragments arranged by size:
3402 1071 901 85 65
BsmI GAATG_Cn'
Cuts at: 3035 3128 3035
Size: 93 5431
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2239 2385 2619 2684 2762 2843 3585
Size: 399 449 146 234 65 78 81 742
Cuts at: 3585 3670 4741 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 449 399 234 146 85
81 78 65
BspEI T'CCGG_A
Cuts at: 5187 5187
Size: 5524
BspHI T'CATG_A
Cuts at: 3413 5143 3413
Size: 1730 3794
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2879 844
Size: 2035 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3411 1394
Size: 429 1588 3507
Fragments arranged by size:
3507 1588 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4019 4193 65
Size: 1183 2771 174 1396
Fragments arranged by size:
2771 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4168 4355 1700
Size: 110 2358 187 2869
Fragments arranged by size:
2869 2358 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5524
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5524
BssSI C'ACGA_G
Cuts at: 2273 2609 2645 3584 4596 2273
Size: 336 36 939 1012 3201
Fragments arranged by size:
3201 1012 939 336 36
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2640 794
Size: 1846 3678
BstBI TT'CG_AA
Cuts at: 4328 4328
Size: 5524
BstEII G'GTnAC_C
Cuts at: 1184 2741 1184
Size: 1557 3967
BstXI CCAn_nnnn'nTGG
Cuts at: 2521 2521
Size: 5524
BstYI r'GATC_y
Cuts at: 1972 2503 2854 3221 3606 3623 5075 5161 5173
Size: 531 351 367 385 17 1452 86 12
Cuts at: 5173 5519 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 531 385 367 351 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2329 2698 513
Size: 552 1264 369 3339
Fragments arranged by size:
3339 1264 552 369
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2416 487
Size: 683 56 1190 3595
Fragments arranged by size:
3595 1190 683 56
BtsI GCAGTG_nn'
Cuts at: 2267 3040 3834 3854 2267
Size: 773 794 20 3937
Fragments arranged by size:
3937 794 773 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5524
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2044 2079 529
Size: 35 1480 35 3974
Fragments arranged by size:
3974 1480 35 35
DraI TTT'AAA
Cuts at: 2828 2828
Size: 5524
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4531 809
Size: 507 3215 1802
Fragments arranged by size:
3215 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2121 2577 2971
Size: 54 1425 50 275 18 291 456 394
Cuts at: 2971 3860 5489 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 456 394 291 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2121 2971 1537
Size: 275 309 850 4090
Fragments arranged by size:
4090 850 309 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2306 2573 2716 3454 1353
Size: 953 267 143 738 3423
Fragments arranged by size:
3423 953 738 267 143
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4111 4485 4631 387
Size: 1064 138 2522 374 146 1280
Fragments arranged by size:
2522 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2617 2617
Size: 5524
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2032 3587 4184 4971 5239 1239
Size: 393 400 1555 597 787 268 1524
Fragments arranged by size:
1555 1524 787 597 400 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2236 2236
Size: 5524
EcoRI G'AATT_C
Cuts at: 2833 3211 2833
Size: 378 5146
FspI TGC'GCA
Cuts at: 4030 4030
Size: 5524
HaeII r_GCGC'y
Cuts at: 1678 2350 4671 1678
Size: 672 2321 2531
Fragments arranged by size:
2531 2321 672
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4165 4197 4239 4271 1373
Size: 32 135 32 2593 32 42 32 2626
Fragments arranged by size:
2626 2593 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2044 2868 3114 669
Size: 988 281 106 824 246 3079
Fragments arranged by size:
3079 988 824 281 246 106
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5524
HpaI GTT'AAC
Cuts at: 3114 3114
Size: 5524
KasI G'GCGC_C
Cuts at: 2346 2346
Size: 5524
KpnI G_GTAC'C
Cuts at: 2706 2849 2706
Size: 143 5381
MfeI C'AATT_G
Cuts at: 3123 3123
Size: 5524
MluI A'CGCG_T
Cuts at: 5400 5400
Size: 5524
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2211 4638 4822 1305
Size: 906 2427 184 2007
Fragments arranged by size:
2427 2007 906 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2579 10
Size: 54 1425 343 747 2955
Fragments arranged by size:
2955 1425 747 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2285 3482 3841 4000 518
Size: 648 1119 1197 359 159 2042
Fragments arranged by size:
2042 1197 1119 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2585 2940 3617 4765 5010 1606
Size: 979 355 677 1148 245 2120
Fragments arranged by size:
2120 1148 979 677 355 245
NarI GG'CG_CC
Cuts at: 2347 2347
Size: 5524
NcoI C'CATG_G
Cuts at: 513 1065 2329 513
Size: 552 1264 3708
Fragments arranged by size:
3708 1264 552
NdeI CA'TA_TG
Cuts at: 387 2395 387
Size: 2008 3516
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5524
NotI GC'GGCC_GC
Cuts at: 2971 2971
Size: 5524
NspI r_CATG'y
Cuts at: 1132 1229 2262 2882 4427 1132
Size: 97 1033 620 1545 2229
Fragments arranged by size:
2229 1545 1033 620 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5524
PciI A'CATG_T
Cuts at: 4423 4423
Size: 5524
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 4622
PmeI GTTT'AAAC
Cuts at: 2828 2828
Size: 5524
PmlI CAC'GTG
Cuts at: 2287 2287
Size: 5524
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3593 3625 5106 5138 3593
Size: 32 1481 32 3979
Fragments arranged by size:
3979 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2396 1613
Size: 783 4741
PsiI TTA'TAA
Cuts at: 3094 3094
Size: 5524
PspOMI G'GGCC_C
Cuts at: 2235 2235
Size: 5524
PstI C_TGCA'G
Cuts at: 830 2244 2405 2876 830
Size: 1414 161 471 3478
Fragments arranged by size:
3478 1414 471 161
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5524
PvuII CAG'CTG
Cuts at: 2585 2585
Size: 5524
SacI G_AGCT'C
Cuts at: 2619 2619
Size: 5524
SalI G'TCGA_C
Cuts at: 1936 2866 1936
Size: 930 4594
SbfI CC_TGCA'GG
Cuts at: 2876 2876
Size: 5524
ScaI AGT'ACT
Cuts at: 2051 3772 5484 2051
Size: 1721 1712 2091
Fragments arranged by size:
2091 1721 1712
SexAI A'CCwGG_T
Cuts at: 2112 2625 2112
Size: 513 5011
SfcI C'TryA_G
Cuts at: 826 1045 2240 2401 2872 4007 4688 4879 826
Size: 219 1195 161 471 1135 681 191 1471
Fragments arranged by size:
1471 1195 1135 681 471 219 191 161
SfoI GGC'GCC
Cuts at: 2348 2348
Size: 5524
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5524
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5524
SmaI CCC'GGG
Cuts at: 1872 2851 1872
Size: 979 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2670 3628 4529 4791 820
Size: 197 926 727 958 901 262 1553
Fragments arranged by size:
1553 958 926 901 727 262 197
SnaBI TAC'GTA
Cuts at: 493 3315 493
Size: 2822 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5524
SphI G_CATG'C
Cuts at: 1132 2882 1132
Size: 1750 3774
SspI AAT'ATT
Cuts at: 5 52 3448 5
Size: 47 3396 2081
Fragments arranged by size:
3396 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5524
StyI C'CwwG_G
Cuts at: 513 1065 2329 2963 513
Size: 552 1264 634 3074
Fragments arranged by size:
3074 1264 634 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3551 3568 3721 3906 4351 325
Size: 1255 1971 17 153 185 445 1498
Fragments arranged by size:
1971 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2049 3770 5482
Size: 274 80 33 51 1443 72 1721 1712
Cuts at: 5482 96
Size: 138
Fragments arranged by size:
1721 1712 1443 274 138 80 72 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5484 5516 5484
Size: 32 5492
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5524
XbaI T'CTAG_A
Cuts at: 2860 2860
Size: 5524
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5524
XmaI C'CCGG_G
Cuts at: 1870 2849 1870
Size: 979 4545
XmnI GAAnn'nnTTC
Cuts at: 2558 3653 2558
Size: 1095 4429
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3332 276
Size: 53 83 186 936 1798 2468
Fragments arranged by size:
2468 1798 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PpuMI PspXI PsrI RsrII SacII SanDI SapI
SfiI SrfI SwaI XcmI