Restriction map for
pFN21AB7110
(Circular) (Six-base) MAPSORT of: pFN21AB7110.seq Check: 6179 from: 1 to: 5485
pFN21AB7110 5485 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3295 278
Size: 53 83 186 936 1759 2468
Fragments arranged by size:
2468 1759 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2828 1937
Size: 891 4594
Acc65I G'GTAC_C
Cuts at: 2806 2806
Size: 5485
AclI AA'CG_TT
Cuts at: 1563 3612 3985 1563
Size: 2049 373 3063
Fragments arranged by size:
3063 2049 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2434 3548 4932 5200 1632
Size: 802 1114 1384 268 1917
Fragments arranged by size:
1917 1384 1114 802 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5288
AflIII A'CryG_T
Cuts at: 1203 4384 5361 1203
Size: 3181 977 1327
Fragments arranged by size:
3181 1327 977
AgeI A'CCGG_T
Cuts at: 4316 4316
Size: 5485
AhdI GACnn_n'nnGTC
Cuts at: 4214 4214
Size: 5485
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5485
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5485
AlwNI CAG_nnn'CTG
Cuts at: 2278 3228 4800 2278
Size: 950 1572 2963
Fragments arranged by size:
2963 1572 950
ApaLI G'TGCA_C
Cuts at: 3542 4698 3542
Size: 1156 4329
ApoI r'AATT_y
Cuts at: 1493 2794 3020 3172 5281 1493
Size: 1301 226 152 2109 1697
Fragments arranged by size:
2109 1697 1301 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2771 4039 160
Size: 872 1739 1268 1606
Fragments arranged by size:
1739 1606 1268 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5485
AvaI C'yCGr_G
Cuts at: 1870 1943 2810 1870
Size: 73 867 4545
Fragments arranged by size:
4545 867 73
BamHI G'GATC_C
Cuts at: 2815 3182 2815
Size: 367 5118
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2093 2392 2635 2806 4261
Size: 325 217 627 306 299 243 171 1455
Cuts at: 4261 618
Size: 1842
Fragments arranged by size:
1842 1455 627 325 306 299 243 217
171
BanII G_rGCy'C
Cuts at: 1391 2313 2804 1391
Size: 922 491 4072
Fragments arranged by size:
4072 922 491
BbeI G_GCGC'C
Cuts at: 2097 2097
Size: 5485
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2425 2662 928
Size: 971 526 237 3751
Fragments arranged by size:
3751 971 526 237
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2312 2346 3676 3710 1903
Size: 34 375 34 1330 34 3678
Fragments arranged by size:
3678 1330 375 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3377 4587 3377
Size: 1210 4275
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5485
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4096 136
Size: 107 122 71 631 605 2424 1525
Fragments arranged by size:
2424 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2400 5480 2400
Size: 3080 2405
BlpI GC'TnA_GC
Cuts at: 2902 2902
Size: 5485
Bme1580I G_kGCm'C
Cuts at: 1790 2640 3546 4702 1790
Size: 850 906 1156 2573
Fragments arranged by size:
2573 1156 906 850
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5485
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2575 3216 4174
Size: 416 53 83 399 24 1158 641 958
Cuts at: 4174 442
Size: 1753
Fragments arranged by size:
1753 1158 958 641 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5485
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4145 1239
Size: 2906 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2456 2603 3610 4475 4773 2456
Size: 147 1007 865 298 3168
Fragments arranged by size:
3168 1007 865 298 147
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4148 882
Size: 617 2649 2219
Fragments arranged by size:
2649 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3276 493
Size: 711 2072 2702
Fragments arranged by size:
2702 2072 711
BsaBI GATnn'nnATC
Cuts at: 5479 5479
Size: 5485
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2094 3292 3674 4309
Size: 53 83 186 936 561 1198 382 635
Cuts at: 4309 275
Size: 1451
Fragments arranged by size:
1451 1198 936 635 561 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3918 4316 4590 4737 5148 3918
Size: 398 274 147 411 4255
Fragments arranged by size:
4255 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2396 2426 1178
Size: 30 165 30 993 30 4237
Fragments arranged by size:
4237 993 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2404 2509 2609 2612 1186
Size: 1218 105 100 3 4059
Fragments arranged by size:
4059 1218 105 100 3
BseYI C'CCAG_C
Cuts at: 4688 5184 4688
Size: 496 4989
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2291 2384 1163
Size: 573 555 93 4264
Fragments arranged by size:
4264 573 555 93
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2935 3696 4724 1540
Size: 275 186 934 761 1028 2301
Fragments arranged by size:
2301 1028 934 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2313 3546 3631 4702 2313
Size: 1233 85 1071 3096
Fragments arranged by size:
3096 1233 1071 85
BsmI GAATG_Cn'
Cuts at: 2335 2996 3089 2335
Size: 661 93 4731
Fragments arranged by size:
4731 661 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2313 2640 2804 3546 3631 4702 1391
Size: 399 523 327 164 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 523 399 327 164 85
BspEI T'CCGG_A
Cuts at: 5148 5148
Size: 5485
BspHI T'CATG_A
Cuts at: 3374 5104 3374
Size: 1730 3755
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2220 2840 844
Size: 1376 620 3489
Fragments arranged by size:
3489 1376 620
BsrBI CCG'CTC
Cuts at: 1394 1823 3372 1394
Size: 429 1549 3507
Fragments arranged by size:
3507 1549 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3980 4154 65
Size: 1183 2732 174 1396
Fragments arranged by size:
2732 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4129 4316 1700
Size: 110 2319 187 2869
Fragments arranged by size:
2869 2319 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5485
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5485
BssSI C'ACGA_G
Cuts at: 3545 4557 3545
Size: 1012 4473
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2460 794
Size: 1666 3819
BstBI TT'CG_AA
Cuts at: 4289 4289
Size: 5485
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5485
BstYI r'GATC_y
Cuts at: 1972 2400 2815 3182 3567 3584 5036 5122 5134
Size: 428 415 367 385 17 1452 86 12
Cuts at: 5134 5480 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 428 415 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2217 2217
Size: 5485
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4933
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2718 487
Size: 683 56 1492 3254
Fragments arranged by size:
3254 1492 683 56
BtsI GCAGTG_nn'
Cuts at: 3001 3795 3815 3001
Size: 794 20 4671
Fragments arranged by size:
4671 794 20
ClaI AT'CG_AT
Cuts at: 1554 2718 1554
Size: 1164 4321
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5450
DraI TTT'AAA
Cuts at: 2789 2789
Size: 5485
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4492 809
Size: 507 3176 1802
Fragments arranged by size:
3176 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2571 2932 3821
Size: 54 1425 50 275 18 741 361 889
Cuts at: 3821 5450 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 741 361 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2932 1537
Size: 275 1120 4090
Fragments arranged by size:
4090 1120 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2422 2471 2498 2673 3415 1353
Size: 1069 49 27 175 742 3423
Fragments arranged by size:
3423 1069 742 175 49 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4072 4446 4592 387
Size: 1064 138 2483 374 146 1280
Fragments arranged by size:
2483 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2311 2311
Size: 5485
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2434 3548 4145 4932 5200 1239
Size: 393 802 1114 597 787 268 1524
Fragments arranged by size:
1524 1114 802 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2216 2282 2591 2216
Size: 66 309 5110
Fragments arranged by size:
5110 309 66
EcoO109I rG'GnC_Cy
Cuts at: 2287 2542 2584 2287
Size: 255 42 5188
Fragments arranged by size:
5188 255 42
EcoRI G'AATT_C
Cuts at: 2794 3172 2794
Size: 378 5107
FspI TGC'GCA
Cuts at: 3991 3991
Size: 5485
HaeII r_GCGC'y
Cuts at: 1678 2097 4632 1678
Size: 419 2535 2531
Fragments arranged by size:
2535 2531 419
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4126 4158 4200 4232 1373
Size: 32 135 32 2554 32 42 32 2626
Fragments arranged by size:
2626 2554 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2829 3075 669
Size: 988 281 891 246 3079
Fragments arranged by size:
3079 988 891 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5485
HpaI GTT'AAC
Cuts at: 3075 3075
Size: 5485
KasI G'GCGC_C
Cuts at: 2093 2093
Size: 5485
KpnI G_GTAC'C
Cuts at: 2810 2810
Size: 5485
MfeI C'AATT_G
Cuts at: 3084 3084
Size: 5485
MluI A'CGCG_T
Cuts at: 5361 5361
Size: 5485
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2034 4599 4783 1305
Size: 729 2565 184 2007
Fragments arranged by size:
2565 2007 729 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2573 10
Size: 54 1425 343 741 2922
Fragments arranged by size:
2922 1425 741 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2733 3443 3802 3961 518
Size: 648 1567 710 359 159 2042
Fragments arranged by size:
2042 1567 710 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2106 2901 3578 4726 4971 1606
Size: 500 795 677 1148 245 2120
Fragments arranged by size:
2120 1148 795 677 500 245
NarI GG'CG_CC
Cuts at: 2094 2094
Size: 5485
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4933
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5485
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5485
NotI GC'GGCC_GC
Cuts at: 2932 2932
Size: 5485
NspI r_CATG'y
Cuts at: 1132 1229 2738 2843 4388 1132
Size: 97 1509 105 1545 2229
Fragments arranged by size:
2229 1545 1509 105 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5485
PciI A'CATG_T
Cuts at: 4384 4384
Size: 5485
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2233 1108
Size: 1125 4360
PfoI T'CCnGG_A
Cuts at: 2226 2226
Size: 5485
PmeI GTTT'AAAC
Cuts at: 2789 2789
Size: 5485
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3554 3586 5067 5099 3554
Size: 32 1481 32 3940
Fragments arranged by size:
3940 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2542 2542
Size: 5485
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5485
PsiI TTA'TAA
Cuts at: 3055 3055
Size: 5485
PstI C_TGCA'G
Cuts at: 830 2295 2837 830
Size: 1465 542 3478
Fragments arranged by size:
3478 1465 542
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5485
SacI G_AGCT'C
Cuts at: 2313 2313
Size: 5485
SalI G'TCGA_C
Cuts at: 1936 2827 1936
Size: 891 4594
SapI GCTCTTCn'nnn_
Cuts at: 2498 2498
Size: 5485
SbfI CC_TGCA'GG
Cuts at: 2295 2837 2295
Size: 542 4943
ScaI AGT'ACT
Cuts at: 3733 5445 3733
Size: 1712 3773
SexAI A'CCwGG_T
Cuts at: 2265 2265
Size: 5485
SfcI C'TryA_G
Cuts at: 826 1045 2291 2404 2833 3968 4649 4840 826
Size: 219 1246 113 429 1135 681 191 1471
Fragments arranged by size:
1471 1246 1135 681 429 219 191 113
SfoI GGC'GCC
Cuts at: 2095 2095
Size: 5485
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5485
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5485
SmaI CCC'GGG
Cuts at: 1872 2812 1872
Size: 940 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2471 2618 3589 4490 4752 820
Size: 197 926 528 147 971 901 262 1553
Fragments arranged by size:
1553 971 926 901 528 262 197 147
SnaBI TAC'GTA
Cuts at: 493 3276 493
Size: 2783 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5485
SphI G_CATG'C
Cuts at: 1132 2843 1132
Size: 1711 3774
SspI AAT'ATT
Cuts at: 5 52 3409 5
Size: 47 3357 2081
Fragments arranged by size:
3357 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5485
StyI C'CwwG_G
Cuts at: 513 1065 2316 2479 2924 513
Size: 552 1251 163 445 3074
Fragments arranged by size:
3074 1251 552 445 163
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2557 2620 2745 3512 3529 3682 3867
Size: 1255 977 63 125 767 17 153 185
Cuts at: 3867 4312 325
Size: 445 1498
Fragments arranged by size:
1498 1255 977 767 445 185 153 125
63 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2601 3731 5443
Size: 274 80 33 51 1443 624 1130 1712
Cuts at: 5443 96
Size: 138
Fragments arranged by size:
1712 1443 1130 624 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5445 5477 5445
Size: 32 5453
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5485
XbaI T'CTAG_A
Cuts at: 2821 2821
Size: 5485
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5485
XmaI C'CCGG_G
Cuts at: 1870 2810 1870
Size: 940 4545
XmnI GAAnn'nnTTC
Cuts at: 3614 3614
Size: 5485
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3293 276
Size: 53 83 186 936 1759 2468
Fragments arranged by size:
2468 1759 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII
SanDI SfiI SrfI SwaI XcmI