Restriction map for
pFN21AB7103
(Circular) (Six-base) MAPSORT of: pFN21AB7103.seq Check: 724 from: 1 to: 5362
pFN21AB7103 5362 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2059 2059
Size: 5362
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3172 278
Size: 53 83 186 936 1636 2468
Fragments arranged by size:
2468 1636 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2705 1937
Size: 768 4594
Acc65I G'GTAC_C
Cuts at: 2683 2683
Size: 5362
AclI AA'CG_TT
Cuts at: 1563 3489 3862 1563
Size: 1926 373 3063
Fragments arranged by size:
3063 1926 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2578 3425 4809 5077 1632
Size: 946 847 1384 268 1917
Fragments arranged by size:
1917 1384 946 847 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5165
AflIII A'CryG_T
Cuts at: 1203 4261 5238 1203
Size: 3058 977 1327
Fragments arranged by size:
3058 1327 977
AgeI A'CCGG_T
Cuts at: 4193 4193
Size: 5362
AhdI GACnn_n'nnGTC
Cuts at: 4091 4091
Size: 5362
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5362
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5362
AlwNI CAG_nnn'CTG
Cuts at: 3105 4677 3105
Size: 1572 3790
ApaLI G'TGCA_C
Cuts at: 3419 4575 3419
Size: 1156 4206
ApoI r'AATT_y
Cuts at: 1493 2671 2897 3049 5158 1493
Size: 1178 226 152 2109 1697
Fragments arranged by size:
2109 1697 1178 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3916 160
Size: 872 2884 1606
Fragments arranged by size:
2884 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5362
AvaI C'yCGr_G
Cuts at: 1870 1943 2687 1870
Size: 73 744 4545
Fragments arranged by size:
4545 744 73
BamHI G'GATC_C
Cuts at: 2692 3059 2692
Size: 367 4995
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2683 4138 618
Size: 325 217 627 896 1455 1842
Fragments arranged by size:
1842 1455 896 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2270 2480 2681 1391
Size: 879 210 201 4072
Fragments arranged by size:
4072 879 210 201
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4391
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3553 3587 1903
Size: 34 1616 34 3678
Fragments arranged by size:
3678 1616 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3254 4464 3254
Size: 1210 4152
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5362
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2405 2577 3973
Size: 107 122 71 631 605 733 172 1396
Cuts at: 3973 136
Size: 1525
Fragments arranged by size:
1525 1396 733 631 605 172 122 107
71
BglII A'GATC_T
Cuts at: 5357 5357
Size: 5362
BlpI GC'TnA_GC
Cuts at: 2779 2779
Size: 5362
Bme1580I G_kGCm'C
Cuts at: 1790 2219 3423 4579 1790
Size: 429 1204 1156 2573
Fragments arranged by size:
2573 1204 1156 429
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5362
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3093 4051 442
Size: 416 53 83 399 24 1676 958 1753
Fragments arranged by size:
1753 1676 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2210 1056
Size: 1154 4208
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4022 1239
Size: 2783 2579
Bpu10I CC'TnA_GC
Cuts at: 2191 2191
Size: 5362
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2007 3487 4352 4650 2007
Size: 1480 865 298 2719
Fragments arranged by size:
2719 1480 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2627 4025 882
Size: 617 1128 1398 2219
Fragments arranged by size:
2219 1398 1128 617
BsaAI yAC'GTr
Cuts at: 493 1204 3153 493
Size: 711 1949 2702
Fragments arranged by size:
2702 1949 711
BsaBI GATnn'nnATC
Cuts at: 5356 5356
Size: 5362
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2343 2626 3169 3551
Size: 53 83 186 936 810 283 543 382
Cuts at: 3551 4186 275
Size: 635 1451
Fragments arranged by size:
1451 936 810 635 543 382 283 186
83 53
BsaWI w'CCGG_w
Cuts at: 3795 4193 4467 4614 5025 3795
Size: 398 274 147 411 4132
Fragments arranged by size:
4132 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2066 2096 2519 2549 1178
Size: 30 165 30 663 30 423 30 3991
Fragments arranged by size:
3991 663 423 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5362
BseYI C'CCAG_C
Cuts at: 2088 2491 4565 5061 2088
Size: 403 2074 496 2389
Fragments arranged by size:
2389 2074 496 403
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2297 1163
Size: 573 561 4228
Fragments arranged by size:
4228 573 561
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2432 2812 3573 4601 1540
Size: 275 186 431 380 761 1028 2301
Fragments arranged by size:
2301 1028 761 431 380 275 186
BsiHKAI G_wGCw'C
Cuts at: 2226 2270 3423 3508 4579 2226
Size: 44 1153 85 1071 3009
Fragments arranged by size:
3009 1153 1071 85 44
BsmI GAATG_Cn'
Cuts at: 2873 2966 2873
Size: 93 5269
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2219 2226 2270 2480 2681 3423 3508
Size: 399 429 7 44 210 201 742 85
Cuts at: 3508 4579 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 429 399 210 201 85
44 7
BspEI T'CCGG_A
Cuts at: 5025 5025
Size: 5362
BspHI T'CATG_A
Cuts at: 3251 4981 3251
Size: 1730 3632
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2059 2717 844
Size: 1215 658 3489
Fragments arranged by size:
3489 1215 658
BsrBI CCG'CTC
Cuts at: 1394 1823 2190 2523 3249 1394
Size: 429 367 333 726 3507
Fragments arranged by size:
3507 726 429 367 333
BsrDI GCAATG_nn'
Cuts at: 65 1248 2457 2646 3857 4031 65
Size: 1183 1209 189 1211 174 1396
Fragments arranged by size:
1396 1211 1209 1183 189 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2384 2622 4006 4193 1700
Size: 110 574 238 1384 187 2869
Fragments arranged by size:
2869 1384 574 238 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5362
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5362
BssSI C'ACGA_G
Cuts at: 2135 2348 2369 2630 3422 4434 2135
Size: 213 21 261 792 1012 3063
Fragments arranged by size:
3063 1012 792 261 213 21
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5362
BstBI TT'CG_AA
Cuts at: 4166 4166
Size: 5362
BstEII G'GTnAC_C
Cuts at: 1184 2380 1184
Size: 1196 4166
BstXI CCAn_nnnn'nTGG
Cuts at: 2545 2545
Size: 5362
BstYI r'GATC_y
Cuts at: 1972 2692 3059 3444 3461 4913 4999 5011 5357
Size: 720 367 385 17 1452 86 12 346
Cuts at: 5357 1972
Size: 1977
Fragments arranged by size:
1977 1452 720 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2096 2127 2572 2581 513
Size: 552 1031 31 445 9 3294
Fragments arranged by size:
3294 1031 552 445 31 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4623
Fragments arranged by size:
4623 683 56
BtsI GCAGTG_nn'
Cuts at: 2165 2332 2527 2878 3672 3692 2165
Size: 167 195 351 794 20 3835
Fragments arranged by size:
3835 794 351 195 167 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5362
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5327
DraI TTT'AAA
Cuts at: 2666 2666
Size: 5362
DraIII CAC_nnn'GTG
Cuts at: 2311 2311
Size: 5362
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4369 809
Size: 507 3053 1802
Fragments arranged by size:
3053 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2124 2397 2406
Size: 54 1425 50 275 18 294 273 9
Cuts at: 2406 2429 2436 2547 2569 2578 2620 2809 3698
Size: 23 7 111 22 9 42 189 889
Cuts at: 3698 5327 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 294 275 273 189 111
54 50 43 42 23 22 18 9
9 7
EagI C'GGCC_G
Cuts at: 1537 1812 2429 2809 1537
Size: 275 617 380 4090
Fragments arranged by size:
4090 617 380 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3292 1353
Size: 1939 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2285 3949 4323 4469 387
Size: 1064 138 696 1664 374 146 1280
Fragments arranged by size:
1664 1280 1064 696 374 146 138
EcoICRI GAG'CTC
Cuts at: 2268 2268
Size: 5362
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2578 3425 4022 4809 5077 1239
Size: 393 946 847 597 787 268 1524
Fragments arranged by size:
1524 946 847 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2671 3049 2671
Size: 378 4984
FspI TGC'GCA
Cuts at: 2324 3868 2324
Size: 1544 3818
HaeII r_GCGC'y
Cuts at: 1678 2104 2111 4509 1678
Size: 426 7 2398 2531
Fragments arranged by size:
2531 2398 426 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2624 2656 4003 4035 4077
Size: 32 135 32 1052 32 1347 32 42
Cuts at: 4077 4109 1373
Size: 32 2626
Fragments arranged by size:
2626 1347 1052 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2175 2706 2952 669
Size: 988 281 237 531 246 3079
Fragments arranged by size:
3079 988 531 281 246 237
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5362
HpaI GTT'AAC
Cuts at: 2952 2952
Size: 5362
KpnI G_GTAC'C
Cuts at: 2687 2687
Size: 5362
MfeI C'AATT_G
Cuts at: 2961 2961
Size: 5362
MluI A'CGCG_T
Cuts at: 5238 5238
Size: 5362
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4476 4660 1305
Size: 3171 184 2007
Fragments arranged by size:
3171 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2549 2571 10
Size: 54 1425 343 717 22 2801
Fragments arranged by size:
2801 1425 717 343 54 22
MslI CAynn'nnrTG
Cuts at: 518 1166 2543 3320 3679 3838 518
Size: 648 1377 777 359 159 2042
Fragments arranged by size:
2042 1377 777 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2098 2260 2434 2778 3455 4603 4848 1606
Size: 492 162 174 344 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 492 344 245 174 162
NaeI GCC'GGC
Cuts at: 2624 2624
Size: 5362
NcoI C'CATG_G
Cuts at: 513 1065 2572 2581 513
Size: 552 1507 9 3294
Fragments arranged by size:
3294 1507 552 9
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5362
NgoMIV G'CCGG_C
Cuts at: 2622 2622
Size: 5362
NheI G'CTAG_C
Cuts at: 1052 2206 1052
Size: 1154 4208
NotI GC'GGCC_GC
Cuts at: 2809 2809
Size: 5362
NspI r_CATG'y
Cuts at: 1132 1229 2059 2720 4265 1132
Size: 97 830 661 1545 2229
Fragments arranged by size:
2229 1545 830 661 97
PasI CC'CwG_GG
Cuts at: 1348 2030 1348
Size: 682 4680
PciI A'CATG_T
Cuts at: 4261 4261
Size: 5362
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2311 1108
Size: 1203 4159
PmeI GTTT'AAAC
Cuts at: 2666 2666
Size: 5362
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3431 3463 4944 4976 3431
Size: 32 1481 32 3817
Fragments arranged by size:
3817 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2611 1613
Size: 998 4364
PsiI TTA'TAA
Cuts at: 2932 2932
Size: 5362
PstI C_TGCA'G
Cuts at: 830 2714 830
Size: 1884 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5362
PvuII CAG'CTG
Cuts at: 2260 2260
Size: 5362
SacI G_AGCT'C
Cuts at: 2270 2270
Size: 5362
SacII CC_GC'GG
Cuts at: 2099 2099
Size: 5362
SalI G'TCGA_C
Cuts at: 1936 2704 1936
Size: 768 4594
SbfI CC_TGCA'GG
Cuts at: 2714 2714
Size: 5362
ScaI AGT'ACT
Cuts at: 3610 5322 3610
Size: 1712 3650
SfcI C'TryA_G
Cuts at: 826 1045 2710 3845 4526 4717 826
Size: 219 1665 1135 681 191 1471
Fragments arranged by size:
1665 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2405 2577 2405
Size: 172 5190
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5362
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5362
SmaI CCC'GGG
Cuts at: 1872 2689 1872
Size: 817 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2022 3466 4367 4629 820
Size: 197 926 79 1444 901 262 1553
Fragments arranged by size:
1553 1444 926 901 262 197 79
SnaBI TAC'GTA
Cuts at: 493 3153 493
Size: 2660 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5362
SphI G_CATG'C
Cuts at: 1132 2720 1132
Size: 1588 3774
SspI AAT'ATT
Cuts at: 5 52 3286 5
Size: 47 3234 2081
Fragments arranged by size:
3234 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5362
StyI C'CwwG_G
Cuts at: 513 1065 2472 2572 2581 2801 513
Size: 552 1407 100 9 220 3074
Fragments arranged by size:
3074 1407 552 220 100 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2504 2576 3389 3406 3559 3744 4189
Size: 1255 924 72 813 17 153 185 445
Cuts at: 4189 325
Size: 1498
Fragments arranged by size:
1498 1255 924 813 445 185 153 72
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3608 5320 96
Size: 274 80 33 51 1443 1631 1712 138
Fragments arranged by size:
1712 1631 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2066 2098 2519 2551 5322 5354 2066
Size: 32 421 32 2771 32 2074
Fragments arranged by size:
2771 2074 421 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5362
XbaI T'CTAG_A
Cuts at: 2698 2698
Size: 5362
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2449 2570 2579 2449
Size: 121 9 5232
Fragments arranged by size:
5232 121 9
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5362
XmaI C'CCGG_G
Cuts at: 1870 2687 1870
Size: 817 4545
XmnI GAAnn'nnTTC
Cuts at: 3491 3491
Size: 5362
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3170 276
Size: 53 83 186 936 1636 2468
Fragments arranged by size:
2468 1636 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SacII SalI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SanDI SapI SexAI SfoI SrfI SwaI