(Circular) (Six-base) MAPSORT of: pFN21AB7092.seq Check: 9342 from: 1 to: 5257 pFN21AB7092 5257 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2038 2038 Size: 5257 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3067 278 Size: 53 83 186 936 1531 2468 Fragments arranged by size: 2468 1531 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2600 1937 Size: 663 4594 Acc65I G'GTAC_C Cuts at: 2578 2578 Size: 5257 AclI AA'CG_TT Cuts at: 1563 3384 3757 1563 Size: 1821 373 3063 Fragments arranged by size: 3063 1821 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2063 2360 3320 4704 4972 1632 Size: 431 297 960 1384 268 1917 Fragments arranged by size: 1917 1384 960 431 297 268 AflII C'TTAA_G Cuts at: 820 1017 2018 820 Size: 197 1001 4059 Fragments arranged by size: 4059 1001 197 AflIII A'CryG_T Cuts at: 1203 2413 4156 5133 1203 Size: 1210 1743 977 1327 Fragments arranged by size: 1743 1327 1210 977 AgeI A'CCGG_T Cuts at: 4088 4088 Size: 5257 AhdI GACnn_n'nnGTC Cuts at: 3986 3986 Size: 5257 AleI CACnn'nnGTG Cuts at: 1166 2483 1166 Size: 1317 3940 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5257 AlwNI CAG_nnn'CTG Cuts at: 2038 3000 4572 2038 Size: 962 1572 2723 Fragments arranged by size: 2723 1572 962 ApaI G_GGCC'C Cuts at: 2423 2423 Size: 5257 ApaLI G'TGCA_C Cuts at: 3314 4470 3314 Size: 1156 4101 ApoI r'AATT_y Cuts at: 1493 2566 2792 2944 5053 1493 Size: 1073 226 152 2109 1697 Fragments arranged by size: 2109 1697 1073 226 152 AseI AT'TA_AT Cuts at: 160 1032 3811 160 Size: 872 2779 1606 Fragments arranged by size: 2779 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5257 AvaI C'yCGr_G Cuts at: 1870 1943 2582 1870 Size: 73 639 4545 Fragments arranged by size: 4545 639 73 BamHI G'GATC_C Cuts at: 2587 2954 2587 Size: 367 4890 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2184 2269 2578 4033 618 Size: 325 217 627 397 85 309 1455 1842 Fragments arranged by size: 1842 1455 627 397 325 309 217 85 BanII G_rGCy'C Cuts at: 1391 2295 2423 2576 1391 Size: 904 128 153 4072 Fragments arranged by size: 4072 904 153 128 BbeI G_GCGC'C Cuts at: 2188 2188 Size: 5257 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4286 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3448 3482 1903 Size: 34 1511 34 3678 Fragments arranged by size: 3678 1511 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3149 4359 3149 Size: 1210 4047 BclI T'GATC_A Cuts at: 1548 1548 Size: 5257 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3868 136 Size: 107 122 71 631 605 2196 1525 Fragments arranged by size: 2196 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5252 5252 Size: 5257 BlpI GC'TnA_GC Cuts at: 2156 2674 2156 Size: 518 4739 Bme1580I G_kGCm'C Cuts at: 1790 2274 2409 2423 3318 4474 1790 Size: 484 135 14 895 1156 2573 Fragments arranged by size: 2573 1156 895 484 135 14 BmgBI CAC'GTC Cuts at: 1318 2428 1318 Size: 1110 4147 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2074 2988 3946 Size: 416 53 83 399 24 657 914 958 Cuts at: 3946 442 Size: 1753 Fragments arranged by size: 1753 958 914 657 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5257 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2309 3917 1239 Size: 1070 1608 2579 Fragments arranged by size: 2579 1608 1070 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2336 3382 4247 4545 2336 Size: 1046 865 298 3048 Fragments arranged by size: 3048 1046 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3920 882 Size: 617 2421 2219 Fragments arranged by size: 2421 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2410 2512 3048 493 Size: 711 1206 102 536 2702 Fragments arranged by size: 2702 1206 711 536 102 BsaBI GATnn'nnATC Cuts at: 5251 5251 Size: 5257 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2185 3064 3446 4081 Size: 53 83 186 936 652 879 382 635 Cuts at: 4081 275 Size: 1451 Fragments arranged by size: 1451 936 879 652 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3690 4088 4362 4509 4920 3690 Size: 398 274 147 411 4027 Fragments arranged by size: 4027 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5032 Fragments arranged by size: 5032 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2339 2543 1186 Size: 1153 204 3900 Fragments arranged by size: 3900 1153 204 BseYI C'CCAG_C Cuts at: 4460 4956 4460 Size: 496 4761 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2072 1163 Size: 573 336 4348 Fragments arranged by size: 4348 573 336 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2235 2707 3468 4496 1540 Size: 275 186 234 472 761 1028 2301 Fragments arranged by size: 2301 1028 761 472 275 234 186 BsiHKAI G_wGCw'C Cuts at: 2295 2349 3318 3403 4474 2295 Size: 54 969 85 1071 3078 Fragments arranged by size: 3078 1071 969 85 54 BsiWI C'GTAC_G Cuts at: 2410 2410 Size: 5257 BsmI GAATG_Cn' Cuts at: 2768 2861 2768 Size: 93 5164 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2274 2295 2349 2409 2423 2576 3318 Size: 399 484 21 54 60 14 153 742 Cuts at: 3318 3403 4474 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 484 399 153 85 60 54 21 14 BspEI T'CCGG_A Cuts at: 4920 4920 Size: 5257 BspHI T'CATG_A Cuts at: 3146 4876 3146 Size: 1730 3527 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2038 2094 2205 2612 844 Size: 1194 56 111 407 3489 Fragments arranged by size: 3489 1194 407 111 56 BsrBI CCG'CTC Cuts at: 1394 1823 3144 1394 Size: 429 1321 3507 Fragments arranged by size: 3507 1321 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3752 3926 65 Size: 1183 2504 174 1396 Fragments arranged by size: 2504 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2115 3901 4088 1700 Size: 110 305 1786 187 2869 Fragments arranged by size: 2869 1786 305 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5257 BssHII G'CGCG_C Cuts at: 1925 2224 1925 Size: 299 4958 BssSI C'ACGA_G Cuts at: 2027 2090 3317 4329 2027 Size: 63 1227 1012 2955 Fragments arranged by size: 2955 1227 1012 63 BstAPI GCAn_nnn'nTGC Cuts at: 794 2038 2095 794 Size: 1244 57 3956 Fragments arranged by size: 3956 1244 57 BstBI TT'CG_AA Cuts at: 4061 4061 Size: 5257 BstEII G'GTnAC_C Cuts at: 1184 2071 2431 1184 Size: 887 360 4010 Fragments arranged by size: 4010 887 360 BstYI r'GATC_y Cuts at: 1972 2587 2954 3339 3356 4808 4894 4906 5252 Size: 615 367 385 17 1452 86 12 346 Cuts at: 5252 1972 Size: 1977 Fragments arranged by size: 1977 1452 615 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2385 513 Size: 552 939 381 3385 Fragments arranged by size: 3385 939 552 381 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2550 487 Size: 683 56 1324 3194 Fragments arranged by size: 3194 1324 683 56 BtsI GCAGTG_nn' Cuts at: 2773 3567 3587 2773 Size: 794 20 4443 Fragments arranged by size: 4443 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5257 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5222 DraI TTT'AAA Cuts at: 2561 2561 Size: 5257 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4264 809 Size: 507 2948 1802 Fragments arranged by size: 2948 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2232 2704 3593 Size: 54 1425 50 275 18 402 472 889 Cuts at: 3593 5222 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 472 402 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2232 2704 1537 Size: 275 420 472 4090 Fragments arranged by size: 4090 472 420 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3187 1353 Size: 1834 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3844 4218 4364 387 Size: 1064 138 2255 374 146 1280 Fragments arranged by size: 2255 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2293 2293 Size: 5257 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2063 2309 2360 3320 3917 4704 4972 Size: 393 431 246 51 960 597 787 268 Cuts at: 4972 1239 Size: 1524 Fragments arranged by size: 1524 960 787 597 431 393 268 246 51 EcoO109I rG'GnC_Cy Cuts at: 2420 2420 Size: 5257 EcoRI G'AATT_C Cuts at: 2566 2944 2566 Size: 378 4879 FspI TGC'GCA Cuts at: 2360 3763 2360 Size: 1403 3854 HaeII r_GCGC'y Cuts at: 1678 2188 4404 1678 Size: 510 2216 2531 Fragments arranged by size: 2531 2216 510 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3898 3930 3972 4004 1373 Size: 32 135 32 2326 32 42 32 2626 Fragments arranged by size: 2626 2326 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2601 2847 669 Size: 988 281 663 246 3079 Fragments arranged by size: 3079 988 663 281 246 HindIII A'AGCT_T Cuts at: 748 2015 748 Size: 1267 3990 HpaI GTT'AAC Cuts at: 2847 2847 Size: 5257 KasI G'GCGC_C Cuts at: 2184 2184 Size: 5257 KpnI G_GTAC'C Cuts at: 2582 2582 Size: 5257 MfeI C'AATT_G Cuts at: 2856 2856 Size: 5257 MluI A'CGCG_T Cuts at: 2413 5133 2413 Size: 2720 2537 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4371 4555 1305 Size: 3066 184 2007 Fragments arranged by size: 3066 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3435 Fragments arranged by size: 3435 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2483 3215 3574 3733 518 Size: 648 1317 732 359 159 2042 Fragments arranged by size: 2042 1317 732 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2125 2155 2673 3350 4498 4743 1606 Size: 519 30 518 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 519 518 245 30 NarI GG'CG_CC Cuts at: 2185 2185 Size: 5257 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3766 Fragments arranged by size: 3766 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5257 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5257 NotI GC'GGCC_GC Cuts at: 2704 2704 Size: 5257 NspI r_CATG'y Cuts at: 1132 1229 2615 4160 1132 Size: 97 1386 1545 2229 Fragments arranged by size: 2229 1545 1386 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5257 PciI A'CATG_T Cuts at: 4156 4156 Size: 5257 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5257 PmeI GTTT'AAAC Cuts at: 2561 2561 Size: 5257 PmlI CAC'GTG Cuts at: 2512 2512 Size: 5257 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3326 3358 4839 4871 3326 Size: 32 1481 32 3712 Fragments arranged by size: 3712 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5257 PsiI TTA'TAA Cuts at: 2827 2827 Size: 5257 PspOMI G'GGCC_C Cuts at: 2419 2419 Size: 5257 PstI C_TGCA'G Cuts at: 830 2124 2609 830 Size: 1294 485 3478 Fragments arranged by size: 3478 1294 485 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5257 PvuII CAG'CTG Cuts at: 2155 2155 Size: 5257 SacI G_AGCT'C Cuts at: 2295 2295 Size: 5257 SalI G'TCGA_C Cuts at: 1936 2599 1936 Size: 663 4594 SbfI CC_TGCA'GG Cuts at: 2609 2609 Size: 5257 ScaI AGT'ACT Cuts at: 3505 5217 3505 Size: 1712 3545 SfcI C'TryA_G Cuts at: 826 1045 2120 2605 3740 4421 4612 826 Size: 219 1075 485 1135 681 191 1471 Fragments arranged by size: 1471 1135 1075 681 485 219 191 SfoI GGC'GCC Cuts at: 2186 2186 Size: 5257 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5257 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5257 SmaI CCC'GGG Cuts at: 1872 2584 1872 Size: 712 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2018 2351 3361 4262 4524 820 Size: 197 926 75 333 1010 901 262 1553 Fragments arranged by size: 1553 1010 926 901 333 262 197 75 SnaBI TAC'GTA Cuts at: 493 3048 493 Size: 2555 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5257 SphI G_CATG'C Cuts at: 1132 2615 1132 Size: 1483 3774 SspI AAT'ATT Cuts at: 5 52 3181 5 Size: 47 3129 2081 Fragments arranged by size: 3129 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5257 StyI C'CwwG_G Cuts at: 513 1065 2004 2696 513 Size: 552 939 692 3074 Fragments arranged by size: 3074 939 692 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2473 3284 3301 3454 3639 4084 325 Size: 1255 893 811 17 153 185 445 1498 Fragments arranged by size: 1498 1255 893 811 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2520 3503 5215 Size: 274 80 33 51 1443 543 983 1712 Cuts at: 5215 96 Size: 138 Fragments arranged by size: 1712 1443 983 543 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5217 5249 5217 Size: 32 5225 Tth111I GACn'n_nGTC Cuts at: 1316 2303 2474 1316 Size: 987 171 4099 Fragments arranged by size: 4099 987 171 XbaI T'CTAG_A Cuts at: 2593 2593 Size: 5257 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5257 XmaI C'CCGG_G Cuts at: 1870 2582 1870 Size: 712 4545 XmnI GAAnn'nnTTC Cuts at: 3386 3386 Size: 5257 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3065 276 Size: 53 83 186 936 1531 2468 Fragments arranged by size: 2468 1531 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI