Restriction map for
pFN21AB7065
(Circular) (Six-base) MAPSORT of: pFN21AB7065.seq Check: 5304 from: 1 to: 7054
pFN21AB7065 7054 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4864 278
Size: 53 83 186 936 3328 2468
Fragments arranged by size:
3328 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4397 1937
Size: 2460 4594
Acc65I G'GTAC_C
Cuts at: 4375 4375
Size: 7054
AclI AA'CG_TT
Cuts at: 1563 5181 5554 1563
Size: 3618 373 3063
Fragments arranged by size:
3618 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2574 4134 5117 6501 6769 1632
Size: 942 1560 983 1384 268 1917
Fragments arranged by size:
1917 1560 1384 983 942 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6857
AflIII A'CryG_T
Cuts at: 1203 2152 5953 6930 1203
Size: 949 3801 977 1327
Fragments arranged by size:
3801 1327 977 949
AgeI A'CCGG_T
Cuts at: 5885 5885
Size: 7054
AhdI GACnn_n'nnGTC
Cuts at: 5783 5783
Size: 7054
AleI CACnn'nnGTG
Cuts at: 1166 3831 1166
Size: 2665 4389
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7054
AlwNI CAG_nnn'CTG
Cuts at: 4797 6369 4797
Size: 1572 5482
ApaLI G'TGCA_C
Cuts at: 5111 6267 5111
Size: 1156 5898
ApoI r'AATT_y
Cuts at: 1493 2125 2264 2272 2376 2392 2460 2796 2880
Size: 632 139 8 104 16 68 336 84
Cuts at: 2880 2964 3132 3216 3300 3384 3468 3587 3746
Size: 84 168 84 84 84 84 119 159
Cuts at: 3746 3888 4056 4140 4308 4363 4589 4741 6850
Size: 142 168 84 168 55 226 152 2109
Cuts at: 6850 1493
Size: 1697
Fragments arranged by size:
2109 1697 632 336 226 168 168 168
159 152 142 139 119 104 84 84
84 84 84 84 84 68 55 16
8
AseI AT'TA_AT
Cuts at: 160 1032 5608 160
Size: 872 4576 1606
Fragments arranged by size:
4576 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7054
AvaI C'yCGr_G
Cuts at: 1870 1943 4379 1870
Size: 73 2436 4545
Fragments arranged by size:
4545 2436 73
BamHI G'GATC_C
Cuts at: 4384 4751 4384
Size: 367 6687
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4375 5830 618
Size: 325 217 627 2588 1455 1842
Fragments arranged by size:
2588 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4373 1391
Size: 2982 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2299 3351 928
Size: 971 400 1052 4631
Fragments arranged by size:
4631 1052 971 400
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5245 5279 1903
Size: 34 3308 34 3678
Fragments arranged by size:
3678 3308 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4946 6156 4946
Size: 1210 5844
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7054
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5665 136
Size: 107 122 71 631 605 3993 1525
Fragments arranged by size:
3993 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7049 7049
Size: 7054
BlpI GC'TnA_GC
Cuts at: 4471 4471
Size: 7054
Bme1580I G_kGCm'C
Cuts at: 1790 2175 5115 6271 1790
Size: 385 2940 1156 2573
Fragments arranged by size:
2940 2573 1156 385
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7054
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2015 4785 5743
Size: 416 53 83 399 24 598 2770 958
Cuts at: 5743 442
Size: 1753
Fragments arranged by size:
2770 1753 958 598 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7054
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2747 2915 3755 2747
Size: 168 840 6046
Fragments arranged by size:
6046 840 168
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2490 2658 2742 2910 3078 3246 3330 3414
Size: 1251 168 84 168 168 168 84 84
Cuts at: 3414 3498 3666 3750 3834 4002 4086 4254 4338
Size: 84 168 84 84 168 84 168 84
Cuts at: 4338 5714 1239
Size: 1376 2579
Fragments arranged by size:
2579 1376 1251 168 168 168 168 168
168 168 84 84 84 84 84 84
84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5179 6044 6342 5179
Size: 865 298 5891
Fragments arranged by size:
5891 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5717 882
Size: 617 4218 2219
Fragments arranged by size:
4218 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4845 493
Size: 711 3641 2702
Fragments arranged by size:
3641 2702 711
BsaBI GATnn'nnATC
Cuts at: 7048 7048
Size: 7054
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4861 5243 5878 275
Size: 53 83 186 936 3328 382 635 1451
Fragments arranged by size:
3328 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5487 5885 6159 6306 6717 5487
Size: 398 274 147 411 5824
Fragments arranged by size:
5824 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6829
Fragments arranged by size:
6829 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3937 1186
Size: 2751 4303
BseYI C'CCAG_C
Cuts at: 2804 6257 6753 2804
Size: 3453 496 3105
Fragments arranged by size:
3453 3105 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2937 3939 4029 1163
Size: 573 1201 1002 90 4188
Fragments arranged by size:
4188 1201 1002 573 90
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4504 5265 6293 1540
Size: 275 186 2503 761 1028 2301
Fragments arranged by size:
2503 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5115 5200 6271 5115
Size: 85 1071 5898
Fragments arranged by size:
5898 1071 85
BsmI GAATG_Cn'
Cuts at: 4565 4658 4565
Size: 93 6961
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2175 4373 5115 5200 6271 1391
Size: 399 385 2198 742 85 1071 2174
Fragments arranged by size:
2198 2174 1071 742 399 385 85
BspEI T'CCGG_A
Cuts at: 6717 6717
Size: 7054
BspHI T'CATG_A
Cuts at: 4943 6673 4943
Size: 1730 5324
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4409 844
Size: 3565 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4941 1394
Size: 429 3118 3507
Fragments arranged by size:
3507 3118 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5549 5723 65
Size: 1183 4301 174 1396
Fragments arranged by size:
4301 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5698 5885 1700
Size: 110 3888 187 2869
Fragments arranged by size:
3888 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7054
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7054
BssSI C'ACGA_G
Cuts at: 5114 6126 5114
Size: 1012 6042
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7054
BstBI TT'CG_AA
Cuts at: 5858 5858
Size: 7054
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7054
BstYI r'GATC_y
Cuts at: 1972 4384 4751 5136 5153 6605 6691 6703 7049
Size: 2412 367 385 17 1452 86 12 346
Cuts at: 7049 1972
Size: 1977
Fragments arranged by size:
2412 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5563
Fragments arranged by size:
5563 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6315
Fragments arranged by size:
6315 683 56
BtsI GCAGTG_nn'
Cuts at: 4570 5364 5384 4570
Size: 794 20 6240
Fragments arranged by size:
6240 794 20
ClaI AT'CG_AT
Cuts at: 1554 3271 1554
Size: 1717 5337
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7019
DraI TTT'AAA
Cuts at: 2596 3604 4358 2596
Size: 1008 754 5292
Fragments arranged by size:
5292 1008 754
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6061 809
Size: 507 4745 1802
Fragments arranged by size:
4745 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2054 4501 5390
Size: 54 1425 50 275 18 224 2447 889
Cuts at: 5390 7019 8
Size: 1629 43
Fragments arranged by size:
2447 1629 1425 889 275 224 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4501 1537
Size: 275 2689 4090
Fragments arranged by size:
4090 2689 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2550 2970 4984 1353
Size: 1197 420 2014 3423
Fragments arranged by size:
3423 2014 1197 420
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5641 6015 6161 387
Size: 1064 138 4052 374 146 1280
Fragments arranged by size:
4052 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2490 2574 2658 2742 2910 3078 3246
Size: 393 858 84 84 84 168 168 168
Cuts at: 3246 3330 3414 3498 3666 3750 3834 4002 4086
Size: 84 84 84 168 84 84 168 84
Cuts at: 4086 4134 4254 4338 5117 5714 6501 6769 1239
Size: 48 120 84 779 597 787 268 1524
Fragments arranged by size:
1524 858 787 779 597 393 268 168
168 168 168 168 120 84 84 84
84 84 84 84 84 84 84 48
EcoRI G'AATT_C
Cuts at: 2376 2392 2460 2796 3468 3888 4363 4741 2376
Size: 16 68 336 672 420 475 378 4689
Fragments arranged by size:
4689 672 475 420 378 336 68 16
FspI TGC'GCA
Cuts at: 5560 5560
Size: 7054
HaeII r_GCGC'y
Cuts at: 1678 6201 1678
Size: 4523 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5695 5727 5769 5801 1373
Size: 32 135 32 4123 32 42 32 2626
Fragments arranged by size:
4123 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2197 4398 4644 669
Size: 988 281 259 2201 246 3079
Fragments arranged by size:
3079 2201 988 281 259 246
HindIII A'AGCT_T
Cuts at: 748 2757 2925 3093 3177 3261 3429 3765 3849
Size: 2009 168 168 84 84 168 336 84
Cuts at: 3849 4017 4101 748
Size: 168 84 3701
Fragments arranged by size:
3701 2009 336 168 168 168 168 84
84 84 84
HpaI GTT'AAC
Cuts at: 2197 4644 2197
Size: 2447 4607
KpnI G_GTAC'C
Cuts at: 4379 4379
Size: 7054
MfeI C'AATT_G
Cuts at: 4653 4653
Size: 7054
MluI A'CGCG_T
Cuts at: 6930 6930
Size: 7054
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6168 6352 1305
Size: 4863 184 2007
Fragments arranged by size:
4863 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2056 10
Size: 54 1425 343 224 5008
Fragments arranged by size:
5008 1425 343 224 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2157 3241 3831 5012 5371 5530 518
Size: 648 991 1084 590 1181 359 159 2042
Fragments arranged by size:
2042 1181 1084 991 648 590 359 159
MspA1I CmG'CkG
Cuts at: 1606 4470 5147 6295 6540 1606
Size: 2864 677 1148 245 2120
Fragments arranged by size:
2864 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5563
Fragments arranged by size:
5563 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7054
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7054
NotI GC'GGCC_GC
Cuts at: 4501 4501
Size: 7054
NspI r_CATG'y
Cuts at: 1132 1229 2156 4412 5957 1132
Size: 97 927 2256 1545 2229
Fragments arranged by size:
2256 2229 1545 927 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7054
PciI A'CATG_T
Cuts at: 2152 5953 2152
Size: 3801 3253
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7054
PmeI GTTT'AAAC
Cuts at: 4358 4358
Size: 7054
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5123 5155 6636 6668 5123
Size: 32 1481 32 5509
Fragments arranged by size:
5509 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7054
PsiI TTA'TAA
Cuts at: 3855 4624 3855
Size: 769 6285
PstI C_TGCA'G
Cuts at: 830 3145 4406 830
Size: 2315 1261 3478
Fragments arranged by size:
3478 2315 1261
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7054
SalI G'TCGA_C
Cuts at: 1936 4396 1936
Size: 2460 4594
SbfI CC_TGCA'GG
Cuts at: 4406 4406
Size: 7054
ScaI AGT'ACT
Cuts at: 5302 7014 5302
Size: 1712 5342
SfcI C'TryA_G
Cuts at: 826 1045 3141 4402 5537 6218 6409 826
Size: 219 2096 1261 1135 681 191 1471
Fragments arranged by size:
2096 1471 1261 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7054
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7054
SmaI CCC'GGG
Cuts at: 1872 4381 1872
Size: 2509 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5158 6059 6321 820
Size: 197 926 3215 901 262 1553
Fragments arranged by size:
3215 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4845 493
Size: 4352 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7054
SphI G_CATG'C
Cuts at: 1132 4412 1132
Size: 3280 3774
SspI AAT'ATT
Cuts at: 5 52 2229 4978 5
Size: 47 2177 2749 2081
Fragments arranged by size:
2749 2177 2081 47
StuI AGG'CCT
Cuts at: 1517 2591 2603 3599 1517
Size: 1074 12 996 4972
Fragments arranged by size:
4972 1074 996 12
StyI C'CwwG_G
Cuts at: 513 1065 2004 4493 513
Size: 552 939 2489 3074
Fragments arranged by size:
3074 2489 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5081 5098 5251 5436 5881 325
Size: 1255 3501 17 153 185 445 1498
Fragments arranged by size:
3501 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5300 7012 96
Size: 274 80 33 51 1443 3323 1712 138
Fragments arranged by size:
3323 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7014 7046 7014
Size: 32 7022
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7054
XbaI T'CTAG_A
Cuts at: 4390 4390
Size: 7054
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7054
XmaI C'CCGG_G
Cuts at: 1870 4379 1870
Size: 2509 4545
XmnI GAAnn'nnTTC
Cuts at: 5183 5183
Size: 7054
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4862 276
Size: 53 83 186 936 3328 2468
Fragments arranged by size:
3328 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI