Restriction map for
pFN21AB7061
(Circular) (Six-base) MAPSORT of: pFN21AB7061.seq Check: 3549 from: 1 to: 6670
pFN21AB7061 6670 bp
With 182 enzymes: SgfI *
February 18, 2014 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4480 278
Size: 53 83 186 936 2944 2468
Fragments arranged by size:
2944 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4013 1937
Size: 2076 4594
Acc65I G'GTAC_C
Cuts at: 3991 3991
Size: 6670
AclI AA'CG_TT
Cuts at: 1563 4797 5170 1563
Size: 3234 373 3063
Fragments arranged by size:
3234 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3314 4733 6117 6385 1632
Size: 1682 1419 1384 268 1917
Fragments arranged by size:
1917 1682 1419 1384 268
AfeI AGC'GCT
Cuts at: 3830 3830
Size: 6670
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6473
AflIII A'CryG_T
Cuts at: 1203 2478 5569 6546 1203
Size: 1275 3091 977 1327
Fragments arranged by size:
3091 1327 1275 977
AgeI A'CCGG_T
Cuts at: 5501 5501
Size: 6670
AhdI GACnn_n'nnGTC
Cuts at: 3340 5399 3340
Size: 2059 4611
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6670
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3215 3615 3738 1184
Size: 2031 400 123 4116
Fragments arranged by size:
4116 2031 400 123
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3803 3835 3803
Size: 32 6638
AlwNI CAG_nnn'CTG
Cuts at: 4413 5985 4413
Size: 1572 5098
ApaI G_GGCC'C
Cuts at: 2342 3503 2342
Size: 1161 5509
ApaLI G'TGCA_C
Cuts at: 2071 4727 5883 2071
Size: 2656 1156 2858
Fragments arranged by size:
2858 2656 1156
ApoI r'AATT_y
Cuts at: 1493 2635 2789 3247 3979 4205 4357 6466 1493
Size: 1142 154 458 732 226 152 2109 1697
Fragments arranged by size:
2109 1697 1142 732 458 226 154 152
AseI AT'TA_AT
Cuts at: 160 1032 5224 160
Size: 872 4192 1606
Fragments arranged by size:
4192 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6670
AvaI C'yCGr_G
Cuts at: 1870 1943 2387 3652 3995 1870
Size: 73 444 1265 343 4545
Fragments arranged by size:
4545 1265 444 343 73
AvrII C'CTAG_G
Cuts at: 2753 2753
Size: 6670
BamHI G'GATC_C
Cuts at: 4000 4367 4000
Size: 367 6303
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2014 2131 2260 2413 3191
Size: 325 217 627 227 117 129 153 778
Cuts at: 3191 3991 5446 618
Size: 800 1455 1842
Fragments arranged by size:
1842 1455 800 778 627 325 227 217
153 129 117
BanII G_rGCy'C
Cuts at: 1391 2342 2531 3302 3503 3989 1391
Size: 951 189 771 201 486 4072
Fragments arranged by size:
4072 951 771 486 201 189
BbeI G_GCGC'C
Cuts at: 2018 2135 2264 2018
Size: 117 129 6424
Fragments arranged by size:
6424 129 117
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3686 928
Size: 971 1787 3912
Fragments arranged by size:
3912 1787 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4861 4895 1903
Size: 34 2924 34 3678
Fragments arranged by size:
3678 2924 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4562 5772 4562
Size: 1210 5460
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6670
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2303 2389 5281
Size: 107 122 71 631 605 631 86 2892
Cuts at: 5281 136
Size: 1525
Fragments arranged by size:
2892 1525 631 631 605 122 107 86
71
BglII A'GATC_T
Cuts at: 2559 6665 2559
Size: 4106 2564
BlpI GC'TnA_GC
Cuts at: 3588 4087 3588
Size: 499 6171
Bme1580I G_kGCm'C
Cuts at: 1790 2075 2342 2418 2589 3503 3727 4731 5887
Size: 285 267 76 171 914 224 1004 1156
Cuts at: 5887 1790
Size: 2573
Fragments arranged by size:
2573 1156 1004 914 285 267 224 171
76
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6670
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3078 3172 4401
Size: 416 53 83 399 24 1661 94 1229
Cuts at: 4401 5359 442
Size: 958 1753
Fragments arranged by size:
1753 1661 1229 958 416 399 94 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6670
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2107 2255 2819 2953 5330 1239
Size: 868 148 564 134 2377 2579
Fragments arranged by size:
2579 2377 868 564 148 134
Bpu10I CC'TnA_GC
Cuts at: 2225 2225
Size: 6670
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4795 5660 5958 4795
Size: 865 298 5507
Fragments arranged by size:
5507 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5333 882
Size: 617 3834 2219
Fragments arranged by size:
3834 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4461 493
Size: 711 3257 2702
Fragments arranged by size:
3257 2702 711
BsaBI GATnn'nnATC
Cuts at: 6664 6664
Size: 6670
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2015 2132 2163 2261
Size: 53 83 186 936 482 117 31 98
Cuts at: 2261 3209 3613 4477 4859 5494 275
Size: 948 404 864 382 635 1451
Fragments arranged by size:
1451 948 936 864 635 482 404 382
186 117 98 83 53 31
BsaWI w'CCGG_w
Cuts at: 5103 5501 5775 5922 6333 5103
Size: 398 274 147 411 5440
Fragments arranged by size:
5440 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6445
Fragments arranged by size:
6445 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2252 3405 3730 1186
Size: 1066 1153 325 4126
Fragments arranged by size:
4126 1153 1066 325
BseYI C'CCAG_C
Cuts at: 2362 2429 2855 3254 3459 3560 3957 5873 6369
Size: 67 426 399 205 101 397 1916 496
Cuts at: 6369 2362
Size: 2663
Fragments arranged by size:
2663 1916 496 426 399 397 205 101
67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2342 2702 1163
Size: 573 606 360 5131
Fragments arranged by size:
5131 606 573 360
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2063 2298 2307 4120 4881 5909
Size: 275 186 62 235 9 1813 761 1028
Cuts at: 5909 1540
Size: 2301
Fragments arranged by size:
2301 1813 1028 761 275 235 186 62
9
BsiHKAI G_wGCw'C
Cuts at: 2075 2531 4731 4816 5887 2075
Size: 456 2200 85 1071 2858
Fragments arranged by size:
2858 2200 1071 456 85
BsmI GAATG_Cn'
Cuts at: 3034 4181 4274 3034
Size: 1147 93 5430
Fragments arranged by size:
5430 1147 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2075 2342 2418 2531 2589 3302 3503
Size: 399 285 267 76 113 58 713 201
Cuts at: 3503 3727 3989 4731 4816 5887 1391
Size: 224 262 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 713 399 285 267 262
224 201 113 85 76 58
BspEI T'CCGG_A
Cuts at: 6333 6333
Size: 6670
BspHI T'CATG_A
Cuts at: 2214 3492 4559 6289 2214
Size: 1278 1067 1730 2595
Fragments arranged by size:
2595 1730 1278 1067
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4025 844
Size: 3181 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2437 2742 4557 1394
Size: 429 614 305 1815 3507
Fragments arranged by size:
3507 1815 614 429 305
BsrDI GCAATG_nn'
Cuts at: 65 1248 2920 3763 5165 5339 65
Size: 1183 1672 843 1402 174 1396
Fragments arranged by size:
1672 1402 1396 1183 843 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2410 5314 5501 1700
Size: 110 600 2904 187 2869
Fragments arranged by size:
2904 2869 600 187 110
BsrGI T'GTAC_A
Cuts at: 96 3688 96
Size: 3592 3078
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6670
BssSI C'ACGA_G
Cuts at: 2068 4730 5742 2068
Size: 2662 1012 2996
Fragments arranged by size:
2996 2662 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6670
BstBI TT'CG_AA
Cuts at: 5474 5474
Size: 6670
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6670
BstYI r'GATC_y
Cuts at: 1972 2559 4000 4367 4752 4769 6221 6307 6319
Size: 587 1441 367 385 17 1452 86 12
Cuts at: 6319 6665 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1441 587 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2019 2019
Size: 6670
BtgI C'CryG_G
Cuts at: 513 1065 2004 2166 2271 2301 2772 513
Size: 552 939 162 105 30 471 4411
Fragments arranged by size:
4411 939 552 471 162 105 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5931
Fragments arranged by size:
5931 683 56
BtsI GCAGTG_nn'
Cuts at: 4186 4980 5000 4186
Size: 794 20 5856
Fragments arranged by size:
5856 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6670
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6635
DraI TTT'AAA
Cuts at: 3974 3974
Size: 6670
DraIII CAC_nnn'GTG
Cuts at: 3935 3935
Size: 6670
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5677 809
Size: 507 4361 1802
Fragments arranged by size:
4361 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2060 2078
Size: 54 1425 50 275 18 177 53 18
Cuts at: 2078 2115 2295 2304 2842 3283 4117 5006 6635
Size: 37 180 9 538 441 834 889 1629
Cuts at: 6635 8
Size: 43
Fragments arranged by size:
1629 1425 889 834 538 441 275 180
177 54 53 50 43 37 18 18
9
EagI C'GGCC_G
Cuts at: 1537 1812 2060 2295 2304 4117 1537
Size: 275 248 235 9 1813 4090
Fragments arranged by size:
4090 1813 275 248 235 9
EarI CTCTTCn'nnn_
Cuts at: 1353 2755 4600 1353
Size: 1402 1845 3423
Fragments arranged by size:
3423 1845 1402
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2549 5257 5631 5777 387
Size: 1064 138 960 2708 374 146 1280
Fragments arranged by size:
2708 1280 1064 960 374 146 138
EcoICRI GAG'CTC
Cuts at: 2529 2529
Size: 6670
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2107 2255 2819 2953 3314 4733 5330
Size: 393 475 148 564 134 361 1419 597
Cuts at: 5330 6117 6385 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1419 787 597 564 475 393 361
268 148 134
EcoO109I rG'GnC_Cy
Cuts at: 2338 3500 2338
Size: 1162 5508
EcoRI G'AATT_C
Cuts at: 2635 3247 3979 4357 2635
Size: 612 732 378 4948
Fragments arranged by size:
4948 732 612 378
FspI TGC'GCA
Cuts at: 5176 5176
Size: 6670
HaeII r_GCGC'y
Cuts at: 1678 2018 2135 2208 2264 2369 3832 5817 1678
Size: 340 117 73 56 105 1463 1985 2531
Fragments arranged by size:
2531 1985 1463 340 117 105 73 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2707 2739 3088 3120 3326
Size: 32 135 32 1135 32 349 32 206
Cuts at: 3326 3358 3833 3865 5311 5343 5385 5417 1373
Size: 32 475 32 1446 32 42 32 2626
Fragments arranged by size:
2626 1446 1135 475 349 206 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3519 3607 4014 4260 669
Size: 988 281 1581 88 407 246 3079
Fragments arranged by size:
3079 1581 988 407 281 246 88
HindIII A'AGCT_T
Cuts at: 748 3922 748
Size: 3174 3496
HpaI GTT'AAC
Cuts at: 3607 4260 3607
Size: 653 6017
KasI G'GCGC_C
Cuts at: 2014 2131 2260 2014
Size: 117 129 6424
Fragments arranged by size:
6424 129 117
KpnI G_GTAC'C
Cuts at: 3995 3995
Size: 6670
MfeI C'AATT_G
Cuts at: 4269 4269
Size: 6670
MluI A'CGCG_T
Cuts at: 2478 6546 2478
Size: 4068 2602
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2133 5784 5968 1305
Size: 828 3651 184 2007
Fragments arranged by size:
3651 2007 828 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2844 3285 10
Size: 54 1425 343 1012 441 3395
Fragments arranged by size:
3395 1425 1012 441 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2678 2913 3197 3716 4628 4987 5146
Size: 648 1512 235 284 519 912 359 159
Cuts at: 5146 518
Size: 2042
Fragments arranged by size:
2042 1512 912 648 519 359 284 235
159
MspA1I CmG'CkG
Cuts at: 1606 2053 2083 2168 2198 2303 2395 2429 3753
Size: 447 30 85 30 105 92 34 1324
Cuts at: 3753 4086 4763 5911 6156 1606
Size: 333 677 1148 245 2120
Fragments arranged by size:
2120 1324 1148 677 447 333 245 105
92 85 34 30 30
NarI GG'CG_CC
Cuts at: 2015 2132 2261 2015
Size: 117 129 6424
Fragments arranged by size:
6424 129 117
NcoI C'CATG_G
Cuts at: 513 1065 2004 2772 513
Size: 552 939 768 4411
Fragments arranged by size:
4411 939 768 552
NdeI CA'TA_TG
Cuts at: 387 3280 387
Size: 2893 3777
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6670
NotI GC'GGCC_GC
Cuts at: 2295 2304 4117 2295
Size: 9 1813 4848
Fragments arranged by size:
4848 1813 9
NspI r_CATG'y
Cuts at: 1132 1229 3587 3820 4028 5573 1132
Size: 97 2358 233 208 1545 2229
Fragments arranged by size:
2358 2229 1545 233 208 97
PasI CC'CwG_GG
Cuts at: 1348 3727 1348
Size: 2379 4291
PciI A'CATG_T
Cuts at: 5569 5569
Size: 6670
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2732 1108
Size: 1624 5046
PmeI GTTT'AAAC
Cuts at: 3974 3974
Size: 6670
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4739 4771 6252 6284 4739
Size: 32 1481 32 5125
Fragments arranged by size:
5125 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6670
PsiI TTA'TAA
Cuts at: 4240 4240
Size: 6670
PspOMI G'GGCC_C
Cuts at: 2338 3499 2338
Size: 1161 5509
PstI C_TGCA'G
Cuts at: 830 3604 4022 830
Size: 2774 418 3478
Fragments arranged by size:
3478 2774 418
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6670
PvuII CAG'CTG
Cuts at: 3753 3753
Size: 6670
SacI G_AGCT'C
Cuts at: 2531 2531
Size: 6670
SacII CC_GC'GG
Cuts at: 2169 2304 2169
Size: 135 6535
SalI G'TCGA_C
Cuts at: 1936 4012 1936
Size: 2076 4594
SapI GCTCTTCn'nnn_
Cuts at: 2755 2755
Size: 6670
SbfI CC_TGCA'GG
Cuts at: 4022 4022
Size: 6670
ScaI AGT'ACT
Cuts at: 2971 4918 6630 2971
Size: 1947 1712 3011
Fragments arranged by size:
3011 1947 1712
SfcI C'TryA_G
Cuts at: 826 1045 3600 4018 5153 5834 6025 826
Size: 219 2555 418 1135 681 191 1471
Fragments arranged by size:
2555 1471 1135 681 418 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2303 2303
Size: 6670
SfoI GGC'GCC
Cuts at: 2016 2133 2262 2016
Size: 117 129 6424
Fragments arranged by size:
6424 129 117
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6670
SgrAI Cr'CCGG_yG
Cuts at: 1700 2410 1700
Size: 710 5960
SmaI CCC'GGG
Cuts at: 1872 2389 3997 1872
Size: 517 1608 4545
Fragments arranged by size:
4545 1608 517
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4774 5675 5937 820
Size: 197 926 2831 901 262 1553
Fragments arranged by size:
2831 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4461 493
Size: 3968 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6670
SphI G_CATG'C
Cuts at: 1132 4028 1132
Size: 2896 3774
SrfI GCCC'GGGC
Cuts at: 2389 2389
Size: 6670
SspI AAT'ATT
Cuts at: 5 52 4594 5
Size: 47 4542 2081
Fragments arranged by size:
4542 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6670
StyI C'CwwG_G
Cuts at: 513 1065 2004 2514 2753 2772 4109 513
Size: 552 939 510 239 19 1337 3074
Fragments arranged by size:
3074 1337 939 552 510 239 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2376 3007 3017 3268 3499 3949 4697
Size: 1255 796 631 10 251 231 450 748
Cuts at: 4697 4714 4867 5052 5497 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 796 748 631 450 445 251
231 185 153 17 10
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2936 2969 3688
Size: 274 80 33 51 1443 959 33 719
Cuts at: 3688 4916 6628 96
Size: 1228 1712 138
Fragments arranged by size:
1712 1443 1228 959 719 274 138 80
51 33 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6630 6662 6630
Size: 32 6638
Tth111I GACn'n_nGTC
Cuts at: 1316 3178 1316
Size: 1862 4808
XbaI T'CTAG_A
Cuts at: 4006 4006
Size: 6670
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2852 3185 3575 2852
Size: 333 390 5947
Fragments arranged by size:
5947 390 333
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6670
XmaI C'CCGG_G
Cuts at: 1870 2387 3995 1870
Size: 517 1608 4545
Fragments arranged by size:
4545 1608 517
XmnI GAAnn'nnTTC
Cuts at: 2704 4799 2704
Size: 2095 4575
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4478 276
Size: 53 83 186 936 2944 2468
Fragments arranged by size:
2944 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI BaeI BbvCI BfrBI BplI BsiWI BsmBI
BstXI BstZ17I EcoNI EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI RsrII SanDI SexAI SwaI