Restriction map for
pFN21AB7056
(Circular) (Six-base) MAPSORT of: pFN21AB7056.seq Check: 2263 from: 1 to: 6457
pFN21AB7056 6457 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3453 3453
Size: 6457
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3096 4267 278
Size: 53 83 186 936 1560 1171 2468
Fragments arranged by size:
2468 1560 1171 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3096 3800 1937
Size: 1159 704 4594
Fragments arranged by size:
4594 1159 704
Acc65I G'GTAC_C
Cuts at: 3778 3778
Size: 6457
AclI AA'CG_TT
Cuts at: 1563 4584 4957 1563
Size: 3021 373 3063
Fragments arranged by size:
3063 3021 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3055 3083 3299 3383 4520 5904 6172 1632
Size: 1423 28 216 84 1137 1384 268 1917
Fragments arranged by size:
1917 1423 1384 1137 268 216 84 28
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6260
AflIII A'CryG_T
Cuts at: 1203 2223 2367 5356 6333 1203
Size: 1020 144 2989 977 1327
Fragments arranged by size:
2989 1327 1020 977 144
AgeI A'CCGG_T
Cuts at: 5288 5288
Size: 6457
AhdI GACnn_n'nnGTC
Cuts at: 3022 5186 3022
Size: 2164 4293
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6457
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2978 1184
Size: 1794 4663
AlwNI CAG_nnn'CTG
Cuts at: 2098 2398 3417 4200 5772 2098
Size: 300 1019 783 1572 2783
Fragments arranged by size:
2783 1572 1019 783 300
ApaI G_GGCC'C
Cuts at: 2675 2931 2675
Size: 256 6201
ApaLI G'TGCA_C
Cuts at: 3284 3380 4514 5670 3284
Size: 96 1134 1156 4071
Fragments arranged by size:
4071 1156 1134 96
ApoI r'AATT_y
Cuts at: 1493 3766 3992 4144 6253 1493
Size: 2273 226 152 2109 1697
Fragments arranged by size:
2273 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5011 160
Size: 872 3979 1606
Fragments arranged by size:
3979 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6457
AvaI C'yCGr_G
Cuts at: 1870 1943 2486 2725 2867 3538 3716 3782 1870
Size: 73 543 239 142 671 178 66 4545
Fragments arranged by size:
4545 671 543 239 178 142 73 66
BamHI G'GATC_C
Cuts at: 2034 3787 4154 2034
Size: 1753 367 4337
Fragments arranged by size:
4337 1753 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2201 2414 3077 3182 3335
Size: 325 217 627 414 213 663 105 153
Cuts at: 3335 3523 3560 3683 3778 5233 618
Size: 188 37 123 95 1455 1842
Fragments arranged by size:
1842 1455 663 627 414 325 217 213
188 153 123 105 95 37
BanII G_rGCy'C
Cuts at: 1391 2675 2931 3776 1391
Size: 1284 256 845 4072
Fragments arranged by size:
4072 1284 845 256
BbeI G_GCGC'C
Cuts at: 2205 2418 3081 3186 3339 3527 3564 3687 2205
Size: 213 663 105 153 188 37 123 4975
Fragments arranged by size:
4975 663 213 188 153 123 105 37
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5486
BbvCI CC'TCA_GC
Cuts at: 2327 2327
Size: 6457
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3409 3443 4648 4682 1903
Size: 34 1472 34 1205 34 3678
Fragments arranged by size:
3678 1472 1205 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4349 5559 4349
Size: 1210 5247
BclI T'GATC_A
Cuts at: 1548 2452 1548
Size: 904 5553
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2285 2570 2621
Size: 107 122 71 631 605 613 285 51
Cuts at: 2621 2630 2795 2820 3657 5068 136
Size: 9 165 25 837 1411 1525
Fragments arranged by size:
1525 1411 837 631 613 605 285 165
122 107 71 51 25 9
BglII A'GATC_T
Cuts at: 6452 6452
Size: 6457
BlpI GC'TnA_GC
Cuts at: 3874 3874
Size: 6457
Bme1580I G_kGCm'C
Cuts at: 1790 2675 2931 3158 3288 3384 4518 5674 1790
Size: 885 256 227 130 96 1134 1156 2573
Fragments arranged by size:
2573 1156 1134 885 256 227 130 96
BmgBI CAC'GTC
Cuts at: 1318 2110 2196 2935 3643 1318
Size: 792 86 739 708 4132
Fragments arranged by size:
4132 792 739 708 86
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4188 5146 442
Size: 416 53 83 399 24 2771 958 1753
Fragments arranged by size:
2771 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6457
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2863 2863
Size: 6457
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2366 5117 1239
Size: 1127 2751 2579
Fragments arranged by size:
2751 2579 1127
Bpu10I CC'TnA_GC
Cuts at: 2327 2327
Size: 6457
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3443 4582 5447 5745 3443
Size: 1139 865 298 4155
Fragments arranged by size:
4155 1139 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2774 5120 882
Size: 617 1275 2346 2219
Fragments arranged by size:
2346 2219 1275 617
BsaAI yAC'GTr
Cuts at: 493 1204 2368 4248 493
Size: 711 1164 1880 2702
Fragments arranged by size:
2702 1880 1164 711
BsaBI GATnn'nnATC
Cuts at: 6451 6451
Size: 6457
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2202 2415 2421 2573
Size: 53 83 186 936 669 213 6 152
Cuts at: 2573 2754 3045 3078 3093 3183 3336 3480 3524
Size: 181 291 33 15 90 153 144 44
Cuts at: 3524 3561 3588 3684 4264 4646 5281 275
Size: 37 27 96 580 382 635 1451
Fragments arranged by size:
1451 936 669 635 580 382 291 213
186 181 153 152 144 96 90 83
53 44 37 33 27 15 6
BsaWI w'CCGG_w
Cuts at: 4890 5288 5562 5709 6120 4890
Size: 398 274 147 411 5227
Fragments arranged by size:
5227 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6232
Fragments arranged by size:
6232 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2456 2849 2852 3035 3708 1186
Size: 1270 393 3 183 673 3935
Fragments arranged by size:
3935 1270 673 393 183 3
BseYI C'CCAG_C
Cuts at: 3533 3570 3575 5660 6156 3533
Size: 37 5 2085 496 3834
Fragments arranged by size:
3834 2085 496 37 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3431 1163
Size: 573 1695 4189
Fragments arranged by size:
4189 1695 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2406 2532 2682 2799 2824 2907
Size: 275 186 405 126 150 117 25 83
Cuts at: 2907 3499 3907 4668 5696 1540
Size: 592 408 761 1028 2301
Fragments arranged by size:
2301 1028 761 592 408 405 275 186
150 126 117 83 25
BsiHKAI G_wGCw'C
Cuts at: 3288 3384 4518 4603 5674 3288
Size: 96 1134 85 1071 4071
Fragments arranged by size:
4071 1134 1071 96 85
BsmI GAATG_Cn'
Cuts at: 3968 4061 3968
Size: 93 6364
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2675 2931 3158 3288 3384 3776 4518
Size: 399 885 256 227 130 96 392 742
Cuts at: 4518 4603 5674 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 885 742 399 392 256 227
130 96 85
BspEI T'CCGG_A
Cuts at: 6120 6120
Size: 6457
BspHI T'CATG_A
Cuts at: 4346 6076 4346
Size: 1730 4727
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3453 3812 844
Size: 2609 359 3489
Fragments arranged by size:
3489 2609 359
BsrBI CCG'CTC
Cuts at: 1394 1823 2151 2686 2743 2903 3435 4344 1394
Size: 429 328 535 57 160 532 909 3507
Fragments arranged by size:
3507 909 535 532 429 328 160 57
BsrDI GCAATG_nn'
Cuts at: 65 1248 4952 5126 65
Size: 1183 3704 174 1396
Fragments arranged by size:
3704 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2009 2388 2782 2794 3185 3666 3686
Size: 110 199 379 394 12 391 481 20
Cuts at: 3686 5101 5288 1700
Size: 1415 187 2869
Fragments arranged by size:
2869 1415 481 394 391 379 199 187
110 20 12
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6457
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6457
BssSI C'ACGA_G
Cuts at: 4517 5529 4517
Size: 1012 5445
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2098 3246 794
Size: 1304 1148 4005
Fragments arranged by size:
4005 1304 1148
BstBI TT'CG_AA
Cuts at: 5261 5261
Size: 6457
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6457
BstYI r'GATC_y
Cuts at: 1972 2034 2349 2394 3129 3787 4154 4539 4556
Size: 62 315 45 735 658 367 385 17
Cuts at: 4556 6008 6094 6106 6452 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 735 658 385 367 346 315
86 62 45 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2205 2526 2555 2607 2628 2634
Size: 552 939 201 321 29 52 21 6
Cuts at: 2634 2824 3000 3493 513
Size: 190 176 493 3477
Fragments arranged by size:
3477 939 552 493 321 201 190 176
52 29 21 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5718
Fragments arranged by size:
5718 683 56
BtsI GCAGTG_nn'
Cuts at: 3335 3973 4767 4787 3335
Size: 638 794 20 5005
Fragments arranged by size:
5005 794 638 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6457
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6422
DraI TTT'AAA
Cuts at: 3761 3761
Size: 6457
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3044 3092 5464 809
Size: 507 1728 48 2372 1802
Fragments arranged by size:
2372 1802 1728 507 48
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2160 2286
Size: 54 1425 50 275 18 177 153 126
Cuts at: 2286 2403 2529 2610 2622 2631 2679 2735 2796
Size: 117 126 81 12 9 48 56 61
Cuts at: 2796 2812 2821 2904 2949 3496 3601 3645 3904
Size: 16 9 83 45 547 105 44 259
Cuts at: 3904 4793 6422 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 547 275 259 177 153
126 126 117 105 83 81 61 56
54 50 48 45 44 43 18 16
12 9 9
EagI C'GGCC_G
Cuts at: 1537 1812 2403 2529 2679 2796 2821 2904 3496
Size: 275 591 126 150 117 25 83 592
Cuts at: 3496 3904 1537
Size: 408 4090
Fragments arranged by size:
4090 592 591 408 275 150 126 117
83 25
EarI CTCTTCn'nnn_
Cuts at: 1353 4387 1353
Size: 3034 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2551 2766 2844 5044 5418 5564
Size: 1064 138 962 215 78 2200 374 146
Cuts at: 5564 387
Size: 1280
Fragments arranged by size:
2200 1280 1064 962 374 215 146 138
78
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2366 3055 3083 3299 3383 4520 5117
Size: 393 734 689 28 216 84 1137 597
Cuts at: 5117 5904 6172 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1137 787 734 689 597 393 268
216 84 28
EcoNI CCTnn'n_nnAGG
Cuts at: 2123 2123
Size: 6457
EcoO109I rG'GnC_Cy
Cuts at: 2095 2672 2095
Size: 577 5880
EcoRI G'AATT_C
Cuts at: 3766 4144 3766
Size: 378 6079
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3274 3274
Size: 6457
FspI TGC'GCA
Cuts at: 4963 4963
Size: 6457
HaeII r_GCGC'y
Cuts at: 1678 2205 2250 2258 2334 2418 3081 3186 3339
Size: 527 45 8 76 84 663 105 153
Cuts at: 3339 3527 3564 3687 5604 1678
Size: 188 37 123 1917 2531
Fragments arranged by size:
2531 1917 663 527 188 153 123 105
84 76 45 37 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3008 3040 5098 5130 5172
Size: 32 135 32 1436 32 2058 32 42
Cuts at: 5172 5204 1373
Size: 32 2626
Fragments arranged by size:
2626 2058 1436 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3801 4047 669
Size: 988 281 1863 246 3079
Fragments arranged by size:
3079 1863 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6457
HpaI GTT'AAC
Cuts at: 4047 4047
Size: 6457
KasI G'GCGC_C
Cuts at: 2201 2414 3077 3182 3335 3523 3560 3683 2201
Size: 213 663 105 153 188 37 123 4975
Fragments arranged by size:
4975 663 213 188 153 123 105 37
KpnI G_GTAC'C
Cuts at: 3782 3782
Size: 6457
MfeI C'AATT_G
Cuts at: 4056 4056
Size: 6457
MluI A'CGCG_T
Cuts at: 6333 6333
Size: 6457
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2835 3462 5571 5755 1305
Size: 1530 627 2109 184 2007
Fragments arranged by size:
2109 2007 1530 627 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2737 3647 10
Size: 54 1425 343 905 910 2820
Fragments arranged by size:
2820 1425 910 905 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3377 4415 4774 4933 518
Size: 648 2211 1038 359 159 2042
Fragments arranged by size:
2211 2042 1038 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2086 2431 2528 2543 2618 2636 2826 3002
Size: 480 345 97 15 75 18 190 176
Cuts at: 3002 3075 3414 3495 3705 3873 4550 5698 5943
Size: 73 339 81 210 168 677 1148 245
Cuts at: 5943 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 480 345 339 245 210
190 176 168 97 81 75 73 18
15
NaeI GCC'GGC
Cuts at: 2011 2796 3187 2011
Size: 785 391 5281
Fragments arranged by size:
5281 785 391
NarI GG'CG_CC
Cuts at: 2202 2415 3078 3183 3336 3524 3561 3684 2202
Size: 213 663 105 153 188 37 123 4975
Fragments arranged by size:
4975 663 213 188 153 123 105 37
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4966
Fragments arranged by size:
4966 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6457
NgoMIV G'CCGG_C
Cuts at: 2009 2794 3185 2009
Size: 785 391 5281
Fragments arranged by size:
5281 785 391
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6457
NotI GC'GGCC_GC
Cuts at: 2529 3496 3904 2529
Size: 967 408 5082
Fragments arranged by size:
5082 967 408
NspI r_CATG'y
Cuts at: 1132 1229 3292 3376 3815 5360 1132
Size: 97 2063 84 439 1545 2229
Fragments arranged by size:
2229 2063 1545 439 97 84
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6457
PciI A'CATG_T
Cuts at: 5356 5356
Size: 6457
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6457
PfoI T'CCnGG_A
Cuts at: 2382 2382
Size: 6457
PmeI GTTT'AAAC
Cuts at: 3761 3761
Size: 6457
PmlI CAC'GTG
Cuts at: 2368 2368
Size: 6457
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4526 4558 6039 6071 4526
Size: 32 1481 32 4912
Fragments arranged by size:
4912 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3428 1613
Size: 1815 4642
PsiI TTA'TAA
Cuts at: 4027 4027
Size: 6457
PspOMI G'GGCC_C
Cuts at: 2671 2927 2671
Size: 256 6201
PspXI vC'TCGA_Gb
Cuts at: 2486 2486
Size: 6457
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2474 2506 2801 2833 2474
Size: 32 295 32 6098
Fragments arranged by size:
6098 295 32 32
PstI C_TGCA'G
Cuts at: 830 2171 2967 3809 830
Size: 1341 796 842 3478
Fragments arranged by size:
3478 1341 842 796
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6457
PvuII CAG'CTG
Cuts at: 2431 3414 2431
Size: 983 5474
SacII CC_GC'GG
Cuts at: 2529 2637 2827 3003 3496 2529
Size: 108 190 176 493 5490
Fragments arranged by size:
5490 493 190 176 108
SalI G'TCGA_C
Cuts at: 1936 3799 1936
Size: 1863 4594
SbfI CC_TGCA'GG
Cuts at: 3809 3809
Size: 6457
ScaI AGT'ACT
Cuts at: 2177 3068 4705 6417 2177
Size: 891 1637 1712 2217
Fragments arranged by size:
2217 1712 1637 891
SfcI C'TryA_G
Cuts at: 826 1045 2167 2963 3805 4940 5621 5812 826
Size: 219 1122 796 842 1135 681 191 1471
Fragments arranged by size:
1471 1135 1122 842 796 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2630 2795 2820 2630
Size: 165 25 6267
Fragments arranged by size:
6267 165 25
SfoI GGC'GCC
Cuts at: 2203 2416 3079 3184 3337 3525 3562 3685 2203
Size: 213 663 105 153 188 37 123 4975
Fragments arranged by size:
4975 663 213 188 153 123 105 37
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6457
SgrAI Cr'CCGG_yG
Cuts at: 1700 2782 3686 1700
Size: 1082 904 4471
Fragments arranged by size:
4471 1082 904
SmaI CCC'GGG
Cuts at: 1872 2727 3540 3718 3784 1872
Size: 855 813 178 66 4545
Fragments arranged by size:
4545 855 813 178 66
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2486 3458 4561 5462 5724 820
Size: 197 926 543 972 1103 901 262 1553
Fragments arranged by size:
1553 1103 972 926 901 543 262 197
SnaBI TAC'GTA
Cuts at: 493 4248 493
Size: 3755 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6457
SphI G_CATG'C
Cuts at: 1132 3815 1132
Size: 2683 3774
SspI AAT'ATT
Cuts at: 5 52 4381 5
Size: 47 4329 2081
Fragments arranged by size:
4329 2081 47
StuI AGG'CCT
Cuts at: 1517 3143 1517
Size: 1626 4831
StyI C'CwwG_G
Cuts at: 513 1065 2004 2475 2598 3138 3896 513
Size: 552 939 471 123 540 758 3074
Fragments arranged by size:
3074 939 758 552 540 471 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2306 3227 4484 4501 4654 4839 5284
Size: 1255 726 921 1257 17 153 185 445
Cuts at: 5284 325
Size: 1498
Fragments arranged by size:
1498 1257 1255 921 726 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2175 3066 4703
Size: 274 80 33 51 1443 198 891 1637
Cuts at: 4703 6415 96
Size: 1712 138
Fragments arranged by size:
1712 1637 1443 891 274 198 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6417 6449 6417
Size: 32 6425
Tth111I GACn'n_nGTC
Cuts at: 1316 2240 1316
Size: 924 5533
XbaI T'CTAG_A
Cuts at: 3793 3793
Size: 6457
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2605 2605
Size: 6457
XhoI C'TCGA_G
Cuts at: 1943 2486 1943
Size: 543 5914
XmaI C'CCGG_G
Cuts at: 1870 2725 3538 3716 3782 1870
Size: 855 813 178 66 4545
Fragments arranged by size:
4545 855 813 178 66
XmnI GAAnn'nnTTC
Cuts at: 2189 3068 4586 2189
Size: 879 1518 4060
Fragments arranged by size:
4060 1518 879
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3094 4265 276
Size: 53 83 186 936 1560 1171 2468
Fragments arranged by size:
2468 1560 1171 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII SacII SalI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI
NruI NsiI PacI PpuMI RsrII SacI SanDI SapI
SexAI SrfI SwaI