Restriction map for
pFN21AB7055
(Circular) (Six-base) MAPSORT of: pFN21AB7055.seq Check: 6396 from: 1 to: 6451
pFN21AB7055 6451 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3256 4261 278
Size: 53 83 186 936 1720 1005 2468
Fragments arranged by size:
2468 1720 1005 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2217 3794 1937
Size: 280 1577 4594
Fragments arranged by size:
4594 1577 280
Acc65I G'GTAC_C
Cuts at: 3772 3772
Size: 6451
AclI AA'CG_TT
Cuts at: 1563 4578 4951 1563
Size: 3015 373 3063
Fragments arranged by size:
3063 3015 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2517 2619 2979 3159 3339 4514 5898 6166
Size: 885 102 360 180 180 1175 1384 268
Cuts at: 6166 1632
Size: 1917
Fragments arranged by size:
1917 1384 1175 885 360 268 180 180
102
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6254
AflIII A'CryG_T
Cuts at: 1203 5350 6327 1203
Size: 4147 977 1327
Fragments arranged by size:
4147 1327 977
AgeI A'CCGG_T
Cuts at: 5282 5282
Size: 6451
AhdI GACnn_n'nnGTC
Cuts at: 5180 5180
Size: 6451
AleI CACnn'nnGTG
Cuts at: 1166 2304 1166
Size: 1138 5313
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3708 1184
Size: 2524 3927
AlwNI CAG_nnn'CTG
Cuts at: 2540 4194 5766 2540
Size: 1654 1572 3225
Fragments arranged by size:
3225 1654 1572
ApaLI G'TGCA_C
Cuts at: 2544 4508 5664 2544
Size: 1964 1156 3331
Fragments arranged by size:
3331 1964 1156
ApoI r'AATT_y
Cuts at: 1493 2638 3177 3760 3986 4138 6247 1493
Size: 1145 539 583 226 152 2109 1697
Fragments arranged by size:
2109 1697 1145 583 539 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5005 160
Size: 872 3973 1606
Fragments arranged by size:
3973 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6451
AvaI C'yCGr_G
Cuts at: 1870 1943 3776 1870
Size: 73 1833 4545
Fragments arranged by size:
4545 1833 73
AvrII C'CTAG_G
Cuts at: 2302 2302
Size: 6451
BamHI G'GATC_C
Cuts at: 2570 3781 4148 2570
Size: 1211 367 4873
Fragments arranged by size:
4873 1211 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3772 5227 618
Size: 325 217 627 1985 1455 1842
Fragments arranged by size:
1985 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2784 3770 1391
Size: 1393 986 4072
Fragments arranged by size:
4072 1393 986
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2865 3735 928
Size: 971 966 870 3644
Fragments arranged by size:
3644 971 966 870
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4642 4676 1903
Size: 34 2705 34 3678
Fragments arranged by size:
3678 2705 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2941 3121 3301 3481 4343 5553 2941
Size: 180 180 180 862 1210 3839
Fragments arranged by size:
3839 1210 862 180 180 180
BclI T'GATC_A
Cuts at: 1548 2227 3703 1548
Size: 679 1476 4296
Fragments arranged by size:
4296 1476 679
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5062 136
Size: 107 122 71 631 605 3390 1525
Fragments arranged by size:
3390 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6446 6446
Size: 6451
BlpI GC'TnA_GC
Cuts at: 3868 3868
Size: 6451
Bme1580I G_kGCm'C
Cuts at: 1790 2548 4512 5668 1790
Size: 758 1964 1156 2573
Fragments arranged by size:
2573 1964 1156 758
BmgBI CAC'GTC
Cuts at: 1318 2680 1318
Size: 1362 5089
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4182 5140 442
Size: 416 53 83 399 24 2765 958 1753
Fragments arranged by size:
2765 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6451
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5111 1239
Size: 3872 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2418 3177 4576 5441 5739 2418
Size: 759 1399 865 298 3130
Fragments arranged by size:
3130 1399 865 759 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2340 5114 882
Size: 617 841 2774 2219
Fragments arranged by size:
2774 2219 841 617
BsaAI yAC'GTr
Cuts at: 493 1204 2879 4242 493
Size: 711 1675 1363 2702
Fragments arranged by size:
2702 1675 1363 711
BsaBI GATnn'nnATC
Cuts at: 6445 6445
Size: 6451
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3253 4258 4640 5275
Size: 53 83 186 936 1720 1005 382 635
Cuts at: 5275 275
Size: 1451
Fragments arranged by size:
1720 1451 1005 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4884 5282 5556 5703 6114 4884
Size: 398 274 147 411 5221
Fragments arranged by size:
5221 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6226
Fragments arranged by size:
6226 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6451
BseYI C'CCAG_C
Cuts at: 5654 6150 5654
Size: 496 5955
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2709 1163
Size: 573 973 4905
Fragments arranged by size:
4905 973 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3901 4662 5690 1540
Size: 275 186 1900 761 1028 2301
Fragments arranged by size:
2301 1900 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2548 2784 3458 4512 4597 5668 2548
Size: 236 674 1054 85 1071 3331
Fragments arranged by size:
3331 1071 1054 674 236 85
BsmI GAATG_Cn'
Cuts at: 2272 3962 4055 2272
Size: 1690 93 4668
Fragments arranged by size:
4668 1690 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3260 3260
Size: 6451
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2548 2784 3458 3770 4512 4597 5668
Size: 399 758 236 674 312 742 85 1071
Cuts at: 5668 1391
Size: 2174
Fragments arranged by size:
2174 1071 758 742 674 399 312 236
85
BspEI T'CCGG_A
Cuts at: 6114 6114
Size: 6451
BspHI T'CATG_A
Cuts at: 2230 3622 4340 6070 2230
Size: 1392 718 1730 2611
Fragments arranged by size:
2611 1730 1392 718
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3806 844
Size: 2962 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4338 1394
Size: 429 2515 3507
Fragments arranged by size:
3507 2515 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4946 5120 65
Size: 1183 3698 174 1396
Fragments arranged by size:
3698 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5095 5282 1700
Size: 110 3285 187 2869
Fragments arranged by size:
3285 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2917 3277 96
Size: 2821 360 3270
Fragments arranged by size:
3270 2821 360
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6451
BssSI C'ACGA_G
Cuts at: 4511 5523 4511
Size: 1012 5439
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6451
BstBI TT'CG_AA
Cuts at: 5255 5255
Size: 6451
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6451
BstYI r'GATC_y
Cuts at: 1972 2108 2570 3781 4148 4533 4550 6002 6088
Size: 136 462 1211 367 385 17 1452 86
Cuts at: 6088 6100 6446 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1211 462 385 367 346 136
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2902 513
Size: 552 1837 4062
Fragments arranged by size:
4062 1837 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5712
Fragments arranged by size:
5712 683 56
BtsI GCAGTG_nn'
Cuts at: 3967 4761 4781 3967
Size: 794 20 5637
Fragments arranged by size:
5637 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6451
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6416
DraI TTT'AAA
Cuts at: 3755 3755
Size: 6451
DraIII CAC_nnn'GTG
Cuts at: 2567 2567
Size: 6451
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5458 809
Size: 507 4142 1802
Fragments arranged by size:
4142 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2842 3022 3202
Size: 54 1425 50 275 18 1012 180 180
Cuts at: 3202 3382 3562 3898 4787 6416 8
Size: 180 180 336 889 1629 43
Fragments arranged by size:
1629 1425 1012 889 336 275 180 180
180 180 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3898 1537
Size: 275 2086 4090
Fragments arranged by size:
4090 2086 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2955 3135 3315 4381 1353
Size: 1602 180 180 1066 3423
Fragments arranged by size:
3423 1602 1066 180 180
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5038 5412 5558 387
Size: 1064 138 3449 374 146 1280
Fragments arranged by size:
3449 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2782 2782
Size: 6451
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2517 2619 2979 3159 3339 4514 5111
Size: 393 885 102 360 180 180 1175 597
Cuts at: 5111 5898 6166 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1175 885 787 597 393 360 268
180 180 102
EcoNI CCTnn'n_nnAGG
Cuts at: 2276 2276
Size: 6451
EcoRI G'AATT_C
Cuts at: 3177 3760 4138 3177
Size: 583 378 5490
Fragments arranged by size:
5490 583 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2127 2127
Size: 6451
FspI TGC'GCA
Cuts at: 4957 4957
Size: 6451
HaeII r_GCGC'y
Cuts at: 1678 5598 1678
Size: 3920 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2410 2442 2803 2835 3523
Size: 32 135 32 838 32 361 32 688
Cuts at: 3523 3542 3555 3574 5092 5124 5166 5198 1373
Size: 19 13 19 1518 32 42 32 2626
Fragments arranged by size:
2626 1518 838 688 361 135 42 32
32 32 32 32 32 19 19 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2315 2614 2677 3795 4041 669
Size: 988 281 377 299 63 1118 246 3079
Fragments arranged by size:
3079 1118 988 377 299 281 246 63
HindIII A'AGCT_T
Cuts at: 748 2529 748
Size: 1781 4670
HpaI GTT'AAC
Cuts at: 2614 4041 2614
Size: 1427 5024
KpnI G_GTAC'C
Cuts at: 3776 3776
Size: 6451
MfeI C'AATT_G
Cuts at: 2096 4050 2096
Size: 1954 4497
MluI A'CGCG_T
Cuts at: 6327 6327
Size: 6451
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2767 5565 5749 1305
Size: 1462 2798 184 2007
Fragments arranged by size:
2798 2007 1462 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3024 3204 3384 3564 10
Size: 54 1425 343 1192 180 180 180 2897
Fragments arranged by size:
2897 1425 1192 343 180 180 180 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2304 4409 4768 4927 518
Size: 648 1138 2105 359 159 2042
Fragments arranged by size:
2105 2042 1138 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2063 3867 4544 5692 5937 1606
Size: 457 1804 677 1148 245 2120
Fragments arranged by size:
2120 1804 1148 677 457 245
NcoI C'CATG_G
Cuts at: 513 1065 2902 513
Size: 552 1837 4062
Fragments arranged by size:
4062 1837 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6451
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6451
NotI GC'GGCC_GC
Cuts at: 3898 3898
Size: 6451
NspI r_CATG'y
Cuts at: 1132 1229 3809 5354 1132
Size: 97 2580 1545 2229
Fragments arranged by size:
2580 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6451
PciI A'CATG_T
Cuts at: 5350 5350
Size: 6451
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2671 1108
Size: 1563 4888
PmeI GTTT'AAAC
Cuts at: 3755 3755
Size: 6451
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4520 4552 6033 6065 4520
Size: 32 1481 32 4906
Fragments arranged by size:
4906 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6451
PsiI TTA'TAA
Cuts at: 2331 4021 2331
Size: 1690 4761
PstI C_TGCA'G
Cuts at: 830 3598 3803 830
Size: 2768 205 3478
Fragments arranged by size:
3478 2768 205
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6451
PvuII CAG'CTG
Cuts at: 2063 2063
Size: 6451
SacI G_AGCT'C
Cuts at: 2784 2784
Size: 6451
SalI G'TCGA_C
Cuts at: 1936 3793 1936
Size: 1857 4594
SapI GCTCTTCn'nnn_
Cuts at: 3135 3135
Size: 6451
SbfI CC_TGCA'GG
Cuts at: 3803 3803
Size: 6451
ScaI AGT'ACT
Cuts at: 4699 6411 4699
Size: 1712 4739
SexAI A'CCwGG_T
Cuts at: 2988 3168 3348 2988
Size: 180 180 6091
Fragments arranged by size:
6091 180 180
SfcI C'TryA_G
Cuts at: 826 1045 3594 3747 3799 4934 5615 5806 826
Size: 219 2549 153 52 1135 681 191 1471
Fragments arranged by size:
2549 1471 1135 681 219 191 153 52
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6451
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6451
SmaI CCC'GGG
Cuts at: 1872 3778 1872
Size: 1906 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2433 3192 4555 5456 5718 820
Size: 197 926 490 759 1363 901 262 1553
Fragments arranged by size:
1553 1363 926 901 759 490 262 197
SnaBI TAC'GTA
Cuts at: 493 4242 493
Size: 3749 2702
SpeI A'CTAG_T
Cuts at: 152 2605 152
Size: 2453 3998
SphI G_CATG'C
Cuts at: 1132 3809 1132
Size: 2677 3774
SspI AAT'ATT
Cuts at: 5 52 3670 4375 5
Size: 47 3618 705 2081
Fragments arranged by size:
3618 2081 705 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6451
StyI C'CwwG_G
Cuts at: 513 1065 2302 2902 3890 513
Size: 552 1237 600 988 3074
Fragments arranged by size:
3074 1237 988 600 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4478 4495 4648 4833 5278 325
Size: 1255 2898 17 153 185 445 1498
Fragments arranged by size:
2898 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2917 3059 3239
Size: 274 80 33 51 1443 940 142 180
Cuts at: 3239 3277 3419 4697 6409 96
Size: 38 142 1278 1712 138
Fragments arranged by size:
1712 1443 1278 940 274 180 142 142
138 80 51 38 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6411 6443 6411
Size: 32 6419
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6451
XbaI T'CTAG_A
Cuts at: 3787 3787
Size: 6451
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2123 2147 2411 2123
Size: 24 264 6163
Fragments arranged by size:
6163 264 24
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6451
XmaI C'CCGG_G
Cuts at: 1870 3776 1870
Size: 1906 4545
XmnI GAAnn'nnTTC
Cuts at: 4580 4580
Size: 6451
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3254 4259 276
Size: 53 83 186 936 1720 1005 2468
Fragments arranged by size:
2468 1720 1005 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SfiI SfoI SrfI SwaI