Restriction map for
pFN21AB7054
(Circular) (Six-base) MAPSORT of: pFN21AB7054.seq Check: 6116 from: 1 to: 6427
pFN21AB7054 6427 bp
With 182 enzymes: SgfI *
July 6, 2012 17:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3431 3431
Size: 6427
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4237 278
Size: 53 83 186 936 2701 2468
Fragments arranged by size:
2701 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3770 1937
Size: 1833 4594
Acc65I G'GTAC_C
Cuts at: 3748 3748
Size: 6427
AclI AA'CG_TT
Cuts at: 1563 4554 4927 1563
Size: 2991 373 3063
Fragments arranged by size:
3063 2991 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3066 4490 5874 6142 1632
Size: 1434 1424 1384 268 1917
Fragments arranged by size:
1917 1434 1424 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6230
AflIII A'CryG_T
Cuts at: 1203 3431 3488 5326 6303 1203
Size: 2228 57 1838 977 1327
Fragments arranged by size:
2228 1838 1327 977 57
AgeI A'CCGG_T
Cuts at: 5258 5258
Size: 6427
AhdI GACnn_n'nnGTC
Cuts at: 5156 5156
Size: 6427
AleI CACnn'nnGTG
Cuts at: 1166 3489 1166
Size: 2323 4104
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6427
AlwNI CAG_nnn'CTG
Cuts at: 2182 2185 2798 3005 4170 5742 2182
Size: 3 613 207 1165 1572 2867
Fragments arranged by size:
2867 1572 1165 613 207 3
ApaI G_GGCC'C
Cuts at: 2134 2524 2134
Size: 390 6037
ApaLI G'TGCA_C
Cuts at: 3482 4484 5640 3482
Size: 1002 1156 4269
Fragments arranged by size:
4269 1156 1002
ApoI r'AATT_y
Cuts at: 1493 3736 3962 4114 6223 1493
Size: 2243 226 152 2109 1697
Fragments arranged by size:
2243 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4981 160
Size: 872 3949 1606
Fragments arranged by size:
3949 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6427
AvaI C'yCGr_G
Cuts at: 1870 1943 2517 3752 1870
Size: 73 574 1235 4545
Fragments arranged by size:
4545 1235 574 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2424 2457 2424
Size: 33 6394
BamHI G'GATC_C
Cuts at: 2941 3757 4124 2941
Size: 816 367 5244
Fragments arranged by size:
5244 816 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2609 2779 3748 5203 618
Size: 325 217 627 822 170 969 1455 1842
Fragments arranged by size:
1842 1455 969 822 627 325 217 170
BanII G_rGCy'C
Cuts at: 1391 2134 2524 3746 1391
Size: 743 390 1222 4072
Fragments arranged by size:
4072 1222 743 390
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3204 3357 3446 928
Size: 971 1305 153 89 3909
Fragments arranged by size:
3909 1305 971 153 89
BbvCI CC'TCA_GC
Cuts at: 2980 2980
Size: 6427
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3284 3318 4618 4652 1903
Size: 34 50 34 1263 34 1300 34 3678
Fragments arranged by size:
3678 1300 1263 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2425 4319 5529 2425
Size: 1894 1210 3323
Fragments arranged by size:
3323 1894 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6427
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2270 2652 5038
Size: 107 122 71 631 605 598 382 2386
Cuts at: 5038 136
Size: 1525
Fragments arranged by size:
2386 1525 631 605 598 382 122 107
71
BglII A'GATC_T
Cuts at: 2069 6422 2069
Size: 4353 2074
BlpI GC'TnA_GC
Cuts at: 3051 3844 3051
Size: 793 5634
Bme1580I G_kGCm'C
Cuts at: 1790 2134 2524 2782 3172 3486 4488 5644 1790
Size: 344 390 258 390 314 1002 1156 2573
Fragments arranged by size:
2573 1156 1002 390 390 344 314 258
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6427
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2248 2659 4158
Size: 416 53 83 399 24 831 411 1499
Cuts at: 4158 5116 442
Size: 958 1753
Fragments arranged by size:
1753 1499 958 831 416 411 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6427
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2688 2967 3000 3018 3171 3239 3369 5087
Size: 1449 279 33 18 153 68 130 1718
Cuts at: 5087 1239
Size: 2579
Fragments arranged by size:
2579 1718 1449 279 153 130 68 33
18
Bpu10I CC'TnA_GC
Cuts at: 2009 2980 2009
Size: 971 5456
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4552 5417 5715 4552
Size: 865 298 5264
Fragments arranged by size:
5264 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2828 5090 882
Size: 617 1329 2262 2219
Fragments arranged by size:
2262 2219 1329 617
BsaAI yAC'GTr
Cuts at: 493 1204 3491 4218 493
Size: 711 2287 727 2702
Fragments arranged by size:
2702 2287 727 711
BsaBI GATnn'nnATC
Cuts at: 6421 6421
Size: 6427
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4234 4616 5251 275
Size: 53 83 186 936 2701 382 635 1451
Fragments arranged by size:
2701 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4860 5258 5532 5679 6090 4860
Size: 398 274 147 411 5197
Fragments arranged by size:
5197 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2192 2222 2576 2606 3144
Size: 30 165 30 789 30 354 30 538
Cuts at: 3144 3174 1178
Size: 30 4431
Fragments arranged by size:
4431 789 538 354 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2567 3168 3236 1186
Size: 1381 601 68 4377
Fragments arranged by size:
4377 1381 601 68
BseYI C'CCAG_C
Cuts at: 2374 2974 2998 3040 3180 5630 6126 2374
Size: 600 24 42 140 2450 496 2675
Fragments arranged by size:
2675 2450 600 496 140 42 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2215 3309 3315 3653 1163
Size: 573 479 1094 6 338 3937
Fragments arranged by size:
3937 1094 573 479 338 6
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3877 4638 5666 1540
Size: 275 186 1876 761 1028 2301
Fragments arranged by size:
2301 1876 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3486 4488 4573 5644 3486
Size: 1002 85 1071 4269
Fragments arranged by size:
4269 1071 1002 85
BsmI GAATG_Cn'
Cuts at: 3938 4031 3938
Size: 93 6334
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2134 2524 2782 3172 3486 3746 4488
Size: 399 344 390 258 390 314 260 742
Cuts at: 4488 4573 5644 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 390 390 344 314
260 258 85
BspEI T'CCGG_A
Cuts at: 6090 6090
Size: 6427
BspHI T'CATG_A
Cuts at: 4316 6046 4316
Size: 1730 4697
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3431 3782 844
Size: 2587 351 3489
Fragments arranged by size:
3489 2587 351
BsrBI CCG'CTC
Cuts at: 1394 1823 2320 4314 1394
Size: 429 497 1994 3507
Fragments arranged by size:
3507 1994 497 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4922 5096 65
Size: 1183 3674 174 1396
Fragments arranged by size:
3674 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5071 5258 1700
Size: 110 3261 187 2869
Fragments arranged by size:
3261 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6427
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6427
BssSI C'ACGA_G
Cuts at: 4487 5499 4487
Size: 1012 5415
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2185 794
Size: 1391 5036
BstBI TT'CG_AA
Cuts at: 5231 5231
Size: 6427
BstEII G'GTnAC_C
Cuts at: 1184 3313 1184
Size: 2129 4298
BstYI r'GATC_y
Cuts at: 1972 2069 2338 2941 3604 3757 4124 4509 4526
Size: 97 269 603 663 153 367 385 17
Cuts at: 4526 5978 6064 6076 6422 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 663 603 385 367 346 269
153 97 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3123 3123
Size: 6427
BtgI C'CryG_G
Cuts at: 513 1065 2605 3477 513
Size: 552 1540 872 3463
Fragments arranged by size:
3463 1540 872 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2025 487
Size: 683 56 799 4889
Fragments arranged by size:
4889 799 683 56
BtsI GCAGTG_nn'
Cuts at: 2888 2954 3165 3682 3943 4737 4757 2888
Size: 66 211 517 261 794 20 4558
Fragments arranged by size:
4558 794 517 261 211 66 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6427
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3415 3450 529
Size: 35 2851 35 3506
Fragments arranged by size:
3506 2851 35 35
DraI TTT'AAA
Cuts at: 3731 3731
Size: 6427
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3106 5434 809
Size: 507 1790 2328 1802
Fragments arranged by size:
2328 1802 1790 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2410 3585 3874
Size: 54 1425 50 275 18 580 1175 289
Cuts at: 3874 4763 6392 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1175 889 580 289 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3874 1537
Size: 275 2062 4090
Fragments arranged by size:
4090 2062 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2863 3090 4357 1353
Size: 1510 227 1267 3423
Fragments arranged by size:
3423 1510 1267 227
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5014 5388 5534 387
Size: 1064 138 3425 374 146 1280
Fragments arranged by size:
3425 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2688 2967 3000 3018 3066 3171 3239
Size: 393 1056 279 33 18 48 105 68
Cuts at: 3239 3369 4490 5087 5874 6142 1239
Size: 130 1121 597 787 268 1524
Fragments arranged by size:
1524 1121 1056 787 597 393 279 268
130 105 68 48 33 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2528 2528
Size: 6427
EcoO109I rG'GnC_Cy
Cuts at: 2119 2131 2521 2909 3128 3544 3580 3617 2119
Size: 12 390 388 219 416 36 37 4929
Fragments arranged by size:
4929 416 390 388 219 37 36 12
EcoRI G'AATT_C
Cuts at: 3736 4114 3736
Size: 378 6049
FspI TGC'GCA
Cuts at: 4933 4933
Size: 6427
HaeII r_GCGC'y
Cuts at: 1678 5574 1678
Size: 3896 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2192 2224 2576 2608 3307
Size: 32 135 32 620 32 352 32 699
Cuts at: 3307 3339 5068 5100 5142 5174 1373
Size: 32 1729 32 42 32 2626
Fragments arranged by size:
2626 1729 699 620 352 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2761 3771 4017 669
Size: 988 281 823 1010 246 3079
Fragments arranged by size:
3079 1010 988 823 281 246
HindIII A'AGCT_T
Cuts at: 748 3391 748
Size: 2643 3784
HpaI GTT'AAC
Cuts at: 4017 4017
Size: 6427
KpnI G_GTAC'C
Cuts at: 3752 3752
Size: 6427
MfeI C'AATT_G
Cuts at: 4026 4026
Size: 6427
MluI A'CGCG_T
Cuts at: 6303 6303
Size: 6427
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2071 5541 5725 1305
Size: 766 3470 184 2007
Fragments arranged by size:
3470 2007 766 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2412 3587 10
Size: 54 1425 343 580 1175 2850
Fragments arranged by size:
2850 1425 1175 580 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2237 2478 3489 4385 4744 4903 518
Size: 648 1071 241 1011 896 359 159 2042
Fragments arranged by size:
2042 1071 1011 896 648 359 241 159
MspA1I CmG'CkG
Cuts at: 1606 2182 2206 2416 2459 3044 3843 4520 5668
Size: 576 24 210 43 585 799 677 1148
Cuts at: 5668 5913 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 799 677 585 576 245 210
43 24
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5875
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6427
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6427
NotI GC'GGCC_GC
Cuts at: 3874 3874
Size: 6427
NspI r_CATG'y
Cuts at: 1132 1229 3435 3476 3785 5330 1132
Size: 97 2206 41 309 1545 2229
Fragments arranged by size:
2229 2206 1545 309 97 41
PasI CC'CwG_GG
Cuts at: 1348 2212 3203 1348
Size: 864 991 4572
Fragments arranged by size:
4572 991 864
PciI A'CATG_T
Cuts at: 3431 5326 3431
Size: 1895 4532
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2260 2381 1108
Size: 1152 121 5154
Fragments arranged by size:
5154 1152 121
PfoI T'CCnGG_A
Cuts at: 2196 2944 3385 2196
Size: 748 441 5238
Fragments arranged by size:
5238 748 441
PmeI GTTT'AAAC
Cuts at: 3731 3731
Size: 6427
PmlI CAC'GTG
Cuts at: 3491 3491
Size: 6427
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4496 4528 6009 6041 4496
Size: 32 1481 32 4882
Fragments arranged by size:
4882 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2119 2909 3128 3544 3580 3617 2119
Size: 790 219 416 36 37 4929
Fragments arranged by size:
4929 790 416 219 37 36
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6427
PsiI TTA'TAA
Cuts at: 3997 3997
Size: 6427
PspOMI G'GGCC_C
Cuts at: 2130 2520 2130
Size: 390 6037
PstI C_TGCA'G
Cuts at: 830 2190 2291 3779 830
Size: 1360 101 1488 3478
Fragments arranged by size:
3478 1488 1360 101
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6427
PvuII CAG'CTG
Cuts at: 2182 2416 2459 3044 2182
Size: 234 43 585 5565
Fragments arranged by size:
5565 585 234 43
SalI G'TCGA_C
Cuts at: 1936 3769 1936
Size: 1833 4594
SanDI GG'GwC_CC
Cuts at: 3617 3617
Size: 6427
SbfI CC_TGCA'GG
Cuts at: 3779 3779
Size: 6427
ScaI AGT'ACT
Cuts at: 2599 4675 6387 2599
Size: 2076 1712 2639
Fragments arranged by size:
2639 2076 1712
SfcI C'TryA_G
Cuts at: 826 1045 2186 2287 3775 4910 5591 5782 826
Size: 219 1141 101 1488 1135 681 191 1471
Fragments arranged by size:
1488 1471 1141 1135 681 219 191 101
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6427
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6427
SmaI CCC'GGG
Cuts at: 1872 2519 3754 1872
Size: 647 1235 4545
Fragments arranged by size:
4545 1235 647
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4531 5432 5694 820
Size: 197 926 2588 901 262 1553
Fragments arranged by size:
2588 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4218 493
Size: 3725 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6427
SphI G_CATG'C
Cuts at: 1132 3785 1132
Size: 2653 3774
SrfI GCCC'GGGC
Cuts at: 2519 2519
Size: 6427
SspI AAT'ATT
Cuts at: 5 52 4351 5
Size: 47 4299 2081
Fragments arranged by size:
4299 2081 47
StuI AGG'CCT
Cuts at: 1517 2054 1517
Size: 537 5890
StyI C'CwwG_G
Cuts at: 513 1065 3866 513
Size: 552 2801 3074
Fragments arranged by size:
3074 2801 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2627 4454 4471 4624 4809 5254 325
Size: 1255 1047 1827 17 153 185 445 1498
Fragments arranged by size:
1827 1498 1255 1047 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2597 4673 6385
Size: 274 80 33 51 1443 620 2076 1712
Cuts at: 6385 96
Size: 138
Fragments arranged by size:
2076 1712 1443 620 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2330 2362 2679 2711 3144 3176 6387 6419 2330
Size: 32 317 32 433 32 3211 32 2338
Fragments arranged by size:
3211 2338 433 317 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2486 1316
Size: 1170 5257
XbaI T'CTAG_A
Cuts at: 3763 3763
Size: 6427
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6427
XmaI C'CCGG_G
Cuts at: 1870 2517 3752 1870
Size: 647 1235 4545
Fragments arranged by size:
4545 1235 647
XmnI GAAnn'nnTTC
Cuts at: 3443 4556 3443
Size: 1113 5314
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4235 276
Size: 53 83 186 936 2701 2468
Fragments arranged by size:
2701 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PspXI PsrI RsrII SacI SacII SapI SexAI SfiI
SfoI SwaI XcmI