Restriction map for
pFN21AB7052
(Circular) (Six-base) MAPSORT of: pFN21AB7052.seq Check: 7204 from: 1 to: 6322
pFN21AB7052 6322 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3279 3345 3279
Size: 66 6256
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4132 278
Size: 53 83 186 936 2596 2468
Fragments arranged by size:
2596 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3665 1937
Size: 1728 4594
Acc65I G'GTAC_C
Cuts at: 3643 3643
Size: 6322
AclI AA'CG_TT
Cuts at: 1563 4449 4822 1563
Size: 2886 373 3063
Fragments arranged by size:
3063 2886 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3191 3621 4385 5769 6037 1632
Size: 1559 430 764 1384 268 1917
Fragments arranged by size:
1917 1559 1384 764 430 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6125
AflIII A'CryG_T
Cuts at: 1203 5221 6198 1203
Size: 4018 977 1327
Fragments arranged by size:
4018 1327 977
AgeI A'CCGG_T
Cuts at: 5153 5153
Size: 6322
AhdI GACnn_n'nnGTC
Cuts at: 5051 5051
Size: 6322
AleI CACnn'nnGTG
Cuts at: 1166 2055 2103 1166
Size: 889 48 5385
Fragments arranged by size:
5385 889 48
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6322
AlwNI CAG_nnn'CTG
Cuts at: 2491 2783 4065 5637 2491
Size: 292 1282 1572 3176
Fragments arranged by size:
3176 1572 1282 292
ApaI G_GGCC'C
Cuts at: 2892 3534 3555 2892
Size: 642 21 5659
Fragments arranged by size:
5659 642 21
ApaLI G'TGCA_C
Cuts at: 4379 5535 4379
Size: 1156 5166
ApoI r'AATT_y
Cuts at: 1493 2315 3631 3857 4009 6118 1493
Size: 822 1316 226 152 2109 1697
Fragments arranged by size:
2109 1697 1316 822 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4876 160
Size: 872 3844 1606
Fragments arranged by size:
3844 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6322
AvaI C'yCGr_G
Cuts at: 1870 1943 2015 3647 1870
Size: 73 72 1632 4545
Fragments arranged by size:
4545 1632 73 72
BamHI G'GATC_C
Cuts at: 3652 4019 3652
Size: 367 5955
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2303 2554 2671 2983 3387
Size: 325 217 627 516 251 117 312 404
Cuts at: 3387 3580 3605 3643 5098 618
Size: 193 25 38 1455 1842
Fragments arranged by size:
1842 1455 627 516 404 325 312 251
217 193 117 38 25
BanII G_rGCy'C
Cuts at: 1391 2015 2064 2482 2892 2918 3534 3555 3641
Size: 624 49 418 410 26 616 21 86
Cuts at: 3641 1391
Size: 4072
Fragments arranged by size:
4072 624 616 418 410 86 49 26
21
BbeI G_GCGC'C
Cuts at: 2307 3391 2307
Size: 1084 5238
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2544 928
Size: 971 645 4706
Fragments arranged by size:
4706 971 645
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4513 4547 1903
Size: 34 2576 34 3678
Fragments arranged by size:
3678 2576 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4214 5424 4214
Size: 1210 5112
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6322
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2094 2587 4933
Size: 107 122 71 631 605 422 493 2346
Cuts at: 4933 136
Size: 1525
Fragments arranged by size:
2346 1525 631 605 493 422 122 107
71
BglII A'GATC_T
Cuts at: 6317 6317
Size: 6322
BlpI GC'TnA_GC
Cuts at: 3739 3739
Size: 6322
Bme1580I G_kGCm'C
Cuts at: 1790 2559 2676 2685 2892 3050 3534 3555 3583
Size: 769 117 9 207 158 484 21 28
Cuts at: 3583 4383 5539 1790
Size: 800 1156 2573
Fragments arranged by size:
2573 1156 800 769 484 207 158 117
28 21 9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6322
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2256 3537 4053
Size: 416 53 83 399 24 839 1281 516
Cuts at: 4053 5011 442
Size: 958 1753
Fragments arranged by size:
1753 1281 958 839 516 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2162 1056
Size: 1106 5216
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2932 2932
Size: 6322
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2354 2408 2513 2541 3389 4982 1239
Size: 1115 54 105 28 848 1593 2579
Fragments arranged by size:
2579 1593 1115 848 105 54 28
Bpu10I CC'TnA_GC
Cuts at: 2066 2957 2066
Size: 891 5431
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3239 4447 5312 5610 3239
Size: 1208 865 298 3951
Fragments arranged by size:
3951 1208 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3544 4985 882
Size: 617 2045 1441 2219
Fragments arranged by size:
2219 2045 1441 617
BsaAI yAC'GTr
Cuts at: 493 1204 4113 493
Size: 711 2909 2702
Fragments arranged by size:
2909 2702 711
BsaBI GATnn'nnATC
Cuts at: 6316 6316
Size: 6322
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2304 3388 4129 4511
Size: 53 83 186 936 771 1084 741 382
Cuts at: 4511 5146 275
Size: 635 1451
Fragments arranged by size:
1451 1084 936 771 741 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2118 4755 5153 5427 5574 5985 2118
Size: 2637 398 274 147 411 2455
Fragments arranged by size:
2637 2455 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2381 2411 2945 2975 1178
Size: 30 165 30 978 30 534 30 4525
Fragments arranged by size:
4525 978 534 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2720 2723 2726 2858 2921 3584 1186
Size: 1534 3 3 132 63 663 3924
Fragments arranged by size:
3924 1534 663 132 63 3 3
BseYI C'CCAG_C
Cuts at: 2092 2450 2860 2911 3226 5525 6021 2092
Size: 358 410 51 315 2299 496 2393
Fragments arranged by size:
2393 2299 496 410 358 315 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2951 3224 3257 1163
Size: 573 1215 273 33 4228
Fragments arranged by size:
4228 1215 573 273 33
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3772 4533 5561 1540
Size: 275 186 1771 761 1028 2301
Fragments arranged by size:
2301 1771 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2064 2370 4383 4468 5539 2064
Size: 306 2013 85 1071 2847
Fragments arranged by size:
2847 2013 1071 306 85
BsmI GAATG_Cn'
Cuts at: 3833 3926 3833
Size: 93 6229
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2064 2370 2482 2559 2676 2685
Size: 399 225 49 306 112 77 117 9
Cuts at: 2685 2892 2918 3050 3534 3555 3583 3641 4383
Size: 207 26 132 484 21 28 58 742
Cuts at: 4383 4468 5539 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 484 399 306 225 207
132 117 112 85 77 58 49 28
26 21 9
BspEI T'CCGG_A
Cuts at: 2118 5985 2118
Size: 3867 2455
BspHI T'CATG_A
Cuts at: 4211 5941 4211
Size: 1730 4592
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3279 3345 3677 844
Size: 2435 66 332 3489
Fragments arranged by size:
3489 2435 332 66
BsrBI CCG'CTC
Cuts at: 1394 1823 3027 4209 1394
Size: 429 1204 1182 3507
Fragments arranged by size:
3507 1204 1182 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4817 4991 65
Size: 1183 3569 174 1396
Fragments arranged by size:
3569 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2261 3285 4966 5153 1700
Size: 110 451 1024 1681 187 2869
Fragments arranged by size:
2869 1681 1024 451 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6322
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6322
BssSI C'ACGA_G
Cuts at: 4382 5394 4382
Size: 1012 5310
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6322
BstBI TT'CG_AA
Cuts at: 5126 5126
Size: 6322
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6322
BstYI r'GATC_y
Cuts at: 1972 2391 3174 3652 4019 4404 4421 5873 5959
Size: 419 783 478 367 385 17 1452 86
Cuts at: 5959 5971 6317 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 783 478 419 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2667 2796 3039 2667
Size: 129 243 5950
Fragments arranged by size:
5950 243 129
BtgI C'CryG_G
Cuts at: 513 1065 2004 2511 3070 3609 513
Size: 552 939 507 559 539 3226
Fragments arranged by size:
3226 939 559 552 539 507
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2389 487
Size: 683 56 1163 4420
Fragments arranged by size:
4420 1163 683 56
BtsI GCAGTG_nn'
Cuts at: 2062 2440 2743 2752 3220 3838 4632 4652 2062
Size: 378 303 9 468 618 794 20 3732
Fragments arranged by size:
3732 794 618 468 378 303 20 9
ClaI AT'CG_AT
Cuts at: 1554 2934 1554
Size: 1380 4942
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6287
DraI TTT'AAA
Cuts at: 3626 3626
Size: 6322
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5329 809
Size: 507 4013 1802
Fragments arranged by size:
4013 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2241 3469 3769
Size: 54 1425 50 275 18 411 1228 300
Cuts at: 3769 4658 6287 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1228 889 411 300 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3769 1537
Size: 275 1957 4090
Fragments arranged by size:
4090 1957 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2835 4252 1353
Size: 1482 1417 3423
Fragments arranged by size:
3423 1482 1417
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2223 2562 3476 4909 5283 5429
Size: 1064 138 634 339 914 1433 374 146
Cuts at: 5429 387
Size: 1280
Fragments arranged by size:
1433 1280 1064 914 634 374 339 146
138
EcoICRI GAG'CTC
Cuts at: 2062 2062
Size: 6322
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2354 2408 2513 2541 3191 3389 3621
Size: 393 722 54 105 28 650 198 232
Cuts at: 3621 4385 4982 5769 6037 1239
Size: 764 597 787 268 1524
Fragments arranged by size:
1524 787 764 722 650 597 393 268
232 198 105 54 28
EcoNI CCTnn'n_nnAGG
Cuts at: 2775 2969 2775
Size: 194 6128
EcoO109I rG'GnC_Cy
Cuts at: 2128 2801 2889 3102 3531 3551 3552 2128
Size: 673 88 213 429 20 1 4898
Fragments arranged by size:
4898 673 429 213 88 20 1
EcoRI G'AATT_C
Cuts at: 3631 4009 3631
Size: 378 5944
FspI TGC'GCA
Cuts at: 3017 3377 4828 3017
Size: 360 1451 4511
Fragments arranged by size:
4511 1451 360
FspAI rTGC'GCAy
Cuts at: 3377 3377
Size: 6322
HaeII r_GCGC'y
Cuts at: 1678 2089 2307 2352 3205 3346 3391 5469 1678
Size: 411 218 45 853 141 45 2078 2531
Fragments arranged by size:
2531 2078 853 411 218 141 45 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4963 4995 5037 5069 1373
Size: 32 135 32 3391 32 42 32 2626
Fragments arranged by size:
3391 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3666 3912 669
Size: 988 281 1728 246 3079
Fragments arranged by size:
3079 1728 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2186 2622 748
Size: 1438 436 4448
Fragments arranged by size:
4448 1438 436
HpaI GTT'AAC
Cuts at: 3912 3912
Size: 6322
KasI G'GCGC_C
Cuts at: 2303 3387 2303
Size: 1084 5238
KpnI G_GTAC'C
Cuts at: 3647 3647
Size: 6322
MfeI C'AATT_G
Cuts at: 3921 3921
Size: 6322
MluI A'CGCG_T
Cuts at: 6198 6198
Size: 6322
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5436 5620 1305
Size: 4131 184 2007
Fragments arranged by size:
4131 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3471 10
Size: 54 1425 343 1639 2861
Fragments arranged by size:
2861 1639 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2055 2103 4280 4639 4798 518
Size: 648 889 48 2177 359 159 2042
Fragments arranged by size:
2177 2042 889 648 359 159 48
MspA1I CmG'CkG
Cuts at: 1606 2092 2363 2896 3434 3449 3738 4415 5563
Size: 486 271 533 538 15 289 677 1148
Cuts at: 5563 5808 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 538 533 486 289 271
245 15
NarI GG'CG_CC
Cuts at: 2304 3388 2304
Size: 1084 5238
NcoI C'CATG_G
Cuts at: 513 1065 2004 3070 3609 513
Size: 552 939 1066 539 3226
Fragments arranged by size:
3226 1066 939 552 539
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6322
NheI G'CTAG_C
Cuts at: 1052 2158 1052
Size: 1106 5216
NotI GC'GGCC_GC
Cuts at: 3769 3769
Size: 6322
NspI r_CATG'y
Cuts at: 1132 1229 2359 3184 3268 3680 5225 1132
Size: 97 1130 825 84 412 1545 2229
Fragments arranged by size:
2229 1545 1130 825 412 97 84
PasI CC'CwG_GG
Cuts at: 1348 3548 1348
Size: 2200 4122
PciI A'CATG_T
Cuts at: 5221 5221
Size: 6322
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2491 2867 1108
Size: 1383 376 4563
Fragments arranged by size:
4563 1383 376
PfoI T'CCnGG_A
Cuts at: 2043 2043
Size: 6322
PmeI GTTT'AAAC
Cuts at: 3626 3626
Size: 6322
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4391 4423 5904 5936 4391
Size: 32 1481 32 4777
Fragments arranged by size:
4777 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2801 3102 2801
Size: 301 6021
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6322
PsiI TTA'TAA
Cuts at: 3892 3892
Size: 6322
PspOMI G'GGCC_C
Cuts at: 2888 3530 3551 2888
Size: 642 21 5659
Fragments arranged by size:
5659 642 21
PstI C_TGCA'G
Cuts at: 830 2430 3342 3674 830
Size: 1600 912 332 3478
Fragments arranged by size:
3478 1600 912 332
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6322
PvuII CAG'CTG
Cuts at: 2092 2363 2092
Size: 271 6051
SacI G_AGCT'C
Cuts at: 2064 2064
Size: 6322
SalI G'TCGA_C
Cuts at: 1936 3664 1936
Size: 1728 4594
SanDI GG'GwC_CC
Cuts at: 2801 2801
Size: 6322
SbfI CC_TGCA'GG
Cuts at: 3674 3674
Size: 6322
ScaI AGT'ACT
Cuts at: 4570 6282 4570
Size: 1712 4610
SfcI C'TryA_G
Cuts at: 826 1045 2167 2426 2764 3338 3670 4805 5486
Size: 219 1122 259 338 574 332 1135 681
Cuts at: 5486 5677 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1122 681 574 338 332 259
219 191
SfoI GGC'GCC
Cuts at: 2305 3389 2305
Size: 1084 5238
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6322
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6322
SmaI CCC'GGG
Cuts at: 1872 3649 1872
Size: 1777 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3254 4426 5327 5589 820
Size: 197 926 1311 1172 901 262 1553
Fragments arranged by size:
1553 1311 1172 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4113 493
Size: 3620 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6322
SphI G_CATG'C
Cuts at: 1132 3680 1132
Size: 2548 3774
SspI AAT'ATT
Cuts at: 5 52 4246 5
Size: 47 4194 2081
Fragments arranged by size:
4194 2081 47
StuI AGG'CCT
Cuts at: 1517 2498 2991 1517
Size: 981 493 4848
Fragments arranged by size:
4848 981 493
StyI C'CwwG_G
Cuts at: 513 1065 2004 2637 3070 3324 3609 3761 513
Size: 552 939 633 433 254 285 152 3074
Fragments arranged by size:
3074 939 633 552 433 285 254 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2159 3598 4349 4366 4519 4704 5149
Size: 1255 579 1439 751 17 153 185 445
Cuts at: 5149 325
Size: 1498
Fragments arranged by size:
1498 1439 1255 751 579 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4568 6280 96
Size: 274 80 33 51 1443 2591 1712 138
Fragments arranged by size:
2591 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2381 2413 6282 6314 2381
Size: 32 3869 32 2389
Fragments arranged by size:
3869 2389 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6322
XbaI T'CTAG_A
Cuts at: 3658 3658
Size: 6322
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3472 3472
Size: 6322
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6322
XmaI C'CCGG_G
Cuts at: 1870 3647 1870
Size: 1777 4545
XmnI GAAnn'nnTTC
Cuts at: 2189 2624 4451 2189
Size: 435 1827 4060
Fragments arranged by size:
4060 1827 435
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4130 276
Size: 53 83 186 936 2596 2468
Fragments arranged by size:
2596 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI
BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI NaeI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII
SacII SapI SexAI SfiI SrfI SwaI