Restriction map for
pFN21AB7051
(Circular) (Six-base) MAPSORT of: pFN21AB7051.seq Check: 12 from: 1 to: 6247
pFN21AB7051 6247 bp
With 182 enzymes: SgfI *
August 20, 2012 11:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4057 278
Size: 53 83 186 936 2521 2468
Fragments arranged by size:
2521 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2103 3590 1937
Size: 166 1487 4594
Fragments arranged by size:
4594 1487 166
Acc65I G'GTAC_C
Cuts at: 3568 3568
Size: 6247
AclI AA'CG_TT
Cuts at: 1563 4374 4747 1563
Size: 2811 373 3063
Fragments arranged by size:
3063 2811 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2206 2436 2955 4310 5694 5962 1632
Size: 574 230 519 1355 1384 268 1917
Fragments arranged by size:
1917 1384 1355 574 519 268 230
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6050
AflIII A'CryG_T
Cuts at: 1203 3147 3228 5146 6123 1203
Size: 1944 81 1918 977 1327
Fragments arranged by size:
1944 1918 1327 977 81
AgeI A'CCGG_T
Cuts at: 5078 5078
Size: 6247
AhdI GACnn_n'nnGTC
Cuts at: 4976 4976
Size: 6247
AleI CACnn'nnGTG
Cuts at: 1166 2778 3263 1166
Size: 1612 485 4150
Fragments arranged by size:
4150 1612 485
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6247
AlwNI CAG_nnn'CTG
Cuts at: 2459 3280 3990 5562 2459
Size: 821 710 1572 3144
Fragments arranged by size:
3144 1572 821 710
ApaLI G'TGCA_C
Cuts at: 3097 4304 5460 3097
Size: 1207 1156 3884
Fragments arranged by size:
3884 1207 1156
ApoI r'AATT_y
Cuts at: 1493 3116 3556 3782 3934 6043 1493
Size: 1623 440 226 152 2109 1697
Fragments arranged by size:
2109 1697 1623 440 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4801 160
Size: 872 3769 1606
Fragments arranged by size:
3769 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6247
AvaI C'yCGr_G
Cuts at: 1870 1943 2468 2651 2909 3572 1870
Size: 73 525 183 258 663 4545
Fragments arranged by size:
4545 663 525 258 183 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2823 2856 2823
Size: 33 6214
BamHI G'GATC_C
Cuts at: 3577 3944 3577
Size: 367 5880
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2260 3568 5023 618
Size: 325 217 627 473 1308 1455 1842
Fragments arranged by size:
1842 1455 1308 627 473 325 217
BanII G_rGCy'C
Cuts at: 1391 3566 1391
Size: 2175 4072
BbeI G_GCGC'C
Cuts at: 2264 2264
Size: 6247
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5276
BbvCI CC'TCA_GC
Cuts at: 2265 2265
Size: 6247
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4438 4472 1903
Size: 34 2501 34 3678
Fragments arranged by size:
3678 2501 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4139 5349 4139
Size: 1210 5037
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6247
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3215 4858 136
Size: 107 122 71 631 605 1543 1643 1525
Fragments arranged by size:
1643 1543 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6242 6242
Size: 6247
BlpI GC'TnA_GC
Cuts at: 2052 3664 2052
Size: 1612 4635
Bme1580I G_kGCm'C
Cuts at: 1790 3101 4308 5464 1790
Size: 1311 1207 1156 2573
Fragments arranged by size:
2573 1311 1207 1156
BmgBI CAC'GTC
Cuts at: 1318 2719 1318
Size: 1401 4846
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2259 3494 3978
Size: 416 53 83 399 24 842 1235 484
Cuts at: 3978 4936 442
Size: 958 1753
Fragments arranged by size:
1753 1235 958 842 484 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6247
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2479 2598 2807 3071 3157 4907 1239
Size: 1240 119 209 264 86 1750 2579
Fragments arranged by size:
2579 1750 1240 264 209 119 86
Bpu10I CC'TnA_GC
Cuts at: 2265 2265
Size: 6247
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4372 5237 5535 4372
Size: 865 298 5084
Fragments arranged by size:
5084 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2654 4910 882
Size: 617 1155 2256 2219
Fragments arranged by size:
2256 2219 1155 617
BsaAI yAC'GTr
Cuts at: 493 1204 2813 3229 4038 493
Size: 711 1609 416 809 2702
Fragments arranged by size:
2702 1609 809 711 416
BsaBI GATnn'nnATC
Cuts at: 6241 6241
Size: 6247
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2261 4054 4436 5071
Size: 53 83 186 936 728 1793 382 635
Cuts at: 5071 275
Size: 1451
Fragments arranged by size:
1793 1451 936 728 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2799 4680 5078 5352 5499 5910 2799
Size: 1881 398 274 147 411 3136
Fragments arranged by size:
3136 1881 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6022
Fragments arranged by size:
6022 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2637 2672 1186
Size: 1451 35 4761
Fragments arranged by size:
4761 1451 35
BseYI C'CCAG_C
Cuts at: 2538 5450 5946 2538
Size: 2912 496 2839
Fragments arranged by size:
2912 2839 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2065 2294 3199 3274 1163
Size: 573 329 229 905 75 4136
Fragments arranged by size:
4136 905 573 329 229 75
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3697 4458 5486 1540
Size: 275 186 1696 761 1028 2301
Fragments arranged by size:
2301 1696 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3101 4308 4393 5464 3101
Size: 1207 85 1071 3884
Fragments arranged by size:
3884 1207 1071 85
BsmI GAATG_Cn'
Cuts at: 2406 3758 3851 2406
Size: 1352 93 4802
Fragments arranged by size:
4802 1352 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3101 3566 4308 4393 5464 1391
Size: 399 1311 465 742 85 1071 2174
Fragments arranged by size:
2174 1311 1071 742 465 399 85
BspEI T'CCGG_A
Cuts at: 2799 5910 2799
Size: 3111 3136
BspHI T'CATG_A
Cuts at: 2733 4136 5866 2733
Size: 1403 1730 3114
Fragments arranged by size:
3114 1730 1403
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2503 3602 844
Size: 1659 1099 3489
Fragments arranged by size:
3489 1659 1099
BsrBI CCG'CTC
Cuts at: 1394 1823 2323 4134 1394
Size: 429 500 1811 3507
Fragments arranged by size:
3507 1811 500 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2902 3030 4742 4916 65
Size: 1183 1654 128 1712 174 1396
Fragments arranged by size:
1712 1654 1396 1183 174 128
BsrFI r'CCGG_y
Cuts at: 1700 1810 4891 5078 1700
Size: 110 3081 187 2869
Fragments arranged by size:
3081 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2244 3150 96
Size: 2148 906 3193
Fragments arranged by size:
3193 2148 906
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6247
BssSI C'ACGA_G
Cuts at: 4307 5319 4307
Size: 1012 5235
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6247
BstBI TT'CG_AA
Cuts at: 5051 5051
Size: 6247
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6247
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2780 3460 2011
Size: 769 680 4798
Fragments arranged by size:
4798 769 680
BstYI r'GATC_y
Cuts at: 1972 2476 2826 3577 3944 4329 4346 5798 5884
Size: 504 350 751 367 385 17 1452 86
Cuts at: 5884 5896 6242 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 751 504 385 367 350 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 2104 2104
Size: 6247
Bsu36I CC'TnA_GG
Cuts at: 2520 2520
Size: 6247
BtgI C'CryG_G
Cuts at: 513 1065 2004 2020 2480 2922 3261 513
Size: 552 939 16 460 442 339 3499
Fragments arranged by size:
3499 939 552 460 442 339 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2112 487
Size: 683 56 886 4622
Fragments arranged by size:
4622 886 683 56
BtsI GCAGTG_nn'
Cuts at: 3472 3763 4557 4577 3472
Size: 291 794 20 5142
Fragments arranged by size:
5142 794 291 20
ClaI AT'CG_AT
Cuts at: 1554 2991 1554
Size: 1437 4810
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2428 2463 529
Size: 35 1864 35 4313
Fragments arranged by size:
4313 1864 35 35
DraI TTT'AAA
Cuts at: 3551 3551
Size: 6247
DraIII CAC_nnn'GTG
Cuts at: 3502 3502
Size: 6247
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2608 5254 809
Size: 507 1292 2646 1802
Fragments arranged by size:
2646 1802 1292 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 2483 3694
Size: 54 1425 50 275 18 183 470 1211
Cuts at: 3694 4583 6212 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1211 889 470 275 183 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3694 1537
Size: 275 1882 4090
Fragments arranged by size:
4090 1882 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2732 3074 4177 1353
Size: 1379 342 1103 3423
Fragments arranged by size:
3423 1379 1103 342
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3100 4834 5208 5354 387
Size: 1064 138 1511 1734 374 146 1280
Fragments arranged by size:
1734 1511 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2206 2436 2479 2598 2807 2955 3071
Size: 393 574 230 43 119 209 148 116
Cuts at: 3071 3157 4310 4907 5694 5962 1239
Size: 86 1153 597 787 268 1524
Fragments arranged by size:
1524 1153 787 597 574 393 268 230
209 148 119 116 86 43
EcoNI CCTnn'n_nnAGG
Cuts at: 3053 3053
Size: 6247
EcoO109I rG'GnC_Cy
Cuts at: 2515 2515
Size: 6247
EcoRI G'AATT_C
Cuts at: 3556 3934 3556
Size: 378 5869
FspI TGC'GCA
Cuts at: 4753 4753
Size: 6247
HaeII r_GCGC'y
Cuts at: 1678 2264 2791 5394 1678
Size: 586 527 2603 2531
Fragments arranged by size:
2603 2531 586 527
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2248 2280 2404 2436 2536
Size: 32 135 32 676 32 124 32 100
Cuts at: 2536 2568 3441 3473 4888 4920 4962 4994 1373
Size: 32 873 32 1415 32 42 32 2626
Fragments arranged by size:
2626 1415 873 676 135 124 100 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3591 3837 669
Size: 988 281 1653 246 3079
Fragments arranged by size:
3079 1653 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3196 748
Size: 2448 3799
HpaI GTT'AAC
Cuts at: 3837 3837
Size: 6247
KasI G'GCGC_C
Cuts at: 2260 2260
Size: 6247
KpnI G_GTAC'C
Cuts at: 3572 3572
Size: 6247
MfeI C'AATT_G
Cuts at: 3846 3846
Size: 6247
MluI A'CGCG_T
Cuts at: 6123 6123
Size: 6247
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2988 5361 5545 1305
Size: 1683 2373 184 2007
Fragments arranged by size:
2373 2007 1683 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2015 10
Size: 54 1425 343 183 4242
Fragments arranged by size:
4242 1425 343 183 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2778 2927 3263 3326 4205 4564 4723
Size: 648 1612 149 336 63 879 359 159
Cuts at: 4723 518
Size: 2042
Fragments arranged by size:
2042 1612 879 648 359 336 159 149
63
MspA1I CmG'CkG
Cuts at: 1606 2542 2903 3663 4340 5488 5733 1606
Size: 936 361 760 677 1148 245 2120
Fragments arranged by size:
2120 1148 936 760 677 361 245
NarI GG'CG_CC
Cuts at: 2261 2261
Size: 6247
NcoI C'CATG_G
Cuts at: 513 1065 2004 2020 2922 3261 513
Size: 552 939 16 902 339 3499
Fragments arranged by size:
3499 939 902 552 339 16
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6247
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6247
NotI GC'GGCC_GC
Cuts at: 3694 3694
Size: 6247
NspI r_CATG'y
Cuts at: 1132 1229 3151 3325 3605 5150 1132
Size: 97 1922 174 280 1545 2229
Fragments arranged by size:
2229 1922 1545 280 174 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6247
PciI A'CATG_T
Cuts at: 3147 5146 3147
Size: 1999 4248
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6247
PfoI T'CCnGG_A
Cuts at: 2495 2495
Size: 6247
PmeI GTTT'AAAC
Cuts at: 3551 3551
Size: 6247
PmlI CAC'GTG
Cuts at: 3229 3229
Size: 6247
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4316 4348 5829 5861 4316
Size: 32 1481 32 4702
Fragments arranged by size:
4702 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2515 2515
Size: 6247
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6247
PsiI TTA'TAA
Cuts at: 3817 3817
Size: 6247
PstI C_TGCA'G
Cuts at: 830 2601 2967 3599 830
Size: 1771 366 632 3478
Fragments arranged by size:
3478 1771 632 366
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6247
PvuII CAG'CTG
Cuts at: 2542 2903 2542
Size: 361 5886
SalI G'TCGA_C
Cuts at: 1936 3589 1936
Size: 1653 4594
SbfI CC_TGCA'GG
Cuts at: 3599 3599
Size: 6247
ScaI AGT'ACT
Cuts at: 2762 4495 6207 2762
Size: 1733 1712 2802
Fragments arranged by size:
2802 1733 1712
SfcI C'TryA_G
Cuts at: 826 1045 2597 2890 2963 3595 4730 5411 5602
Size: 219 1552 293 73 632 1135 681 191
Cuts at: 5602 826
Size: 1471
Fragments arranged by size:
1552 1471 1135 681 632 293 219 191
73
SfoI GGC'GCC
Cuts at: 2262 2262
Size: 6247
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6247
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6247
SmaI CCC'GGG
Cuts at: 1872 2653 3574 1872
Size: 781 921 4545
Fragments arranged by size:
4545 921 781
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4351 5252 5514 820
Size: 197 926 2408 901 262 1553
Fragments arranged by size:
2408 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4038 493
Size: 3545 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6247
SphI G_CATG'C
Cuts at: 1132 3605 1132
Size: 2473 3774
SspI AAT'ATT
Cuts at: 5 52 4171 5
Size: 47 4119 2081
Fragments arranged by size:
4119 2081 47
StuI AGG'CCT
Cuts at: 1517 2525 1517
Size: 1008 5239
StyI C'CwwG_G
Cuts at: 513 1065 2004 2020 2922 3261 3686 513
Size: 552 939 16 902 339 425 3074
Fragments arranged by size:
3074 939 902 552 425 339 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3513 4274 4291 4444 4629 5074 325
Size: 1255 1933 761 17 153 185 445 1498
Fragments arranged by size:
1933 1498 1255 761 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2244 2760 3150
Size: 274 80 33 51 1443 267 516 390
Cuts at: 3150 4493 6205 96
Size: 1343 1712 138
Fragments arranged by size:
1712 1443 1343 516 390 274 267 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2620 2652 2935 2967 6207 6239 2620
Size: 32 283 32 3240 32 2628
Fragments arranged by size:
3240 2628 283 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6247
XbaI T'CTAG_A
Cuts at: 3583 3583
Size: 6247
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6247
XmaI C'CCGG_G
Cuts at: 1870 2651 3572 1870
Size: 781 921 4545
Fragments arranged by size:
4545 921 781
XmnI GAAnn'nnTTC
Cuts at: 3170 4376 3170
Size: 1206 5041
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4055 276
Size: 53 83 186 936 2521 2468
Fragments arranged by size:
2521 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BfrBI BplI
BsiWI BsmBI EcoICRI EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SrfI SwaI
XcmI