Restriction map for
pFN21AB7050
(Circular) (Six-base) MAPSORT of: pFN21AB7050.seq Check: 4900 from: 1 to: 6217
pFN21AB7050 6217 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4027 278
Size: 53 83 186 936 2491 2468
Fragments arranged by size:
2491 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3560 1937
Size: 1623 4594
Acc65I G'GTAC_C
Cuts at: 2113 3538 2113
Size: 1425 4792
AclI AA'CG_TT
Cuts at: 1563 4344 4717 1563
Size: 2781 373 3063
Fragments arranged by size:
3063 2781 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3478 4280 5664 5932 1632
Size: 1846 802 1384 268 1917
Fragments arranged by size:
1917 1846 1384 802 268
AfeI AGC'GCT
Cuts at: 2328 2345 2328
Size: 17 6200
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6020
AflIII A'CryG_T
Cuts at: 1203 5116 6093 1203
Size: 3913 977 1327
Fragments arranged by size:
3913 1327 977
AgeI A'CCGG_T
Cuts at: 5048 5048
Size: 6217
AhdI GACnn_n'nnGTC
Cuts at: 4946 4946
Size: 6217
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6217
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6217
AlwNI CAG_nnn'CTG
Cuts at: 3960 5532 3960
Size: 1572 4645
ApaLI G'TGCA_C
Cuts at: 2380 4274 5430 2380
Size: 1894 1156 3167
Fragments arranged by size:
3167 1894 1156
ApoI r'AATT_y
Cuts at: 1493 3526 3752 3904 6013 1493
Size: 2033 226 152 2109 1697
Fragments arranged by size:
2109 2033 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4771 160
Size: 872 3739 1606
Fragments arranged by size:
3739 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
AvaI C'yCGr_G
Cuts at: 1870 1943 2209 2237 2463 2488 3542 1870
Size: 73 266 28 226 25 1054 4545
Fragments arranged by size:
4545 1054 266 226 73 28 25
AvrII C'CTAG_G
Cuts at: 2746 2746
Size: 6217
BamHI G'GATC_C
Cuts at: 3547 3914 3547
Size: 367 5850
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2113 2594 3538 4993 618
Size: 325 217 627 326 481 944 1455 1842
Fragments arranged by size:
1842 1455 944 627 481 326 325 217
BanII G_rGCy'C
Cuts at: 1391 2496 3536 1391
Size: 1105 1040 4072
Fragments arranged by size:
4072 1105 1040
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5246
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2371 2405 4408 4442 1903
Size: 34 434 34 2003 34 3678
Fragments arranged by size:
3678 2003 434 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2843 4109 5319 2843
Size: 1266 1210 3741
Fragments arranged by size:
3741 1266 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6217
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2423 4828 136
Size: 107 122 71 631 605 751 2405 1525
Fragments arranged by size:
2405 1525 751 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6212 6212
Size: 6217
BlpI GC'TnA_GC
Cuts at: 3634 3634
Size: 6217
Bme1580I G_kGCm'C
Cuts at: 1790 2384 4278 5434 1790
Size: 594 1894 1156 2573
Fragments arranged by size:
2573 1894 1156 594
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6217
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2600 3948 4906
Size: 416 53 83 399 24 1183 1348 958
Cuts at: 4906 442
Size: 1753
Fragments arranged by size:
1753 1348 1183 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6217
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2264 2871 4877 1239
Size: 1025 607 2006 2579
Fragments arranged by size:
2579 2006 1025 607
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2315 2620 2959 4342 5207 5505 2315
Size: 305 339 1383 865 298 3027
Fragments arranged by size:
3027 1383 865 339 305 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2025 4880 882
Size: 617 526 2855 2219
Fragments arranged by size:
2855 2219 617 526
BsaAI yAC'GTr
Cuts at: 493 1204 4008 493
Size: 711 2804 2702
Fragments arranged by size:
2804 2702 711
BsaBI GATnn'nnATC
Cuts at: 6211 6211
Size: 6217
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2285 4024 4406 5041
Size: 53 83 186 936 752 1739 382 635
Cuts at: 5041 275
Size: 1451
Fragments arranged by size:
1739 1451 936 752 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3514 4650 5048 5322 5469 5880 3514
Size: 1136 398 274 147 411 3851
Fragments arranged by size:
3851 1136 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2463 2493 1178
Size: 30 165 30 1060 30 4902
Fragments arranged by size:
4902 1060 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2465 2832 3225 1186
Size: 1279 367 393 4178
Fragments arranged by size:
4178 1279 393 367
BseYI C'CCAG_C
Cuts at: 2137 2156 2584 5420 5916 2137
Size: 19 428 2836 496 2438
Fragments arranged by size:
2836 2438 496 428 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5644
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2050 2153 2181 2187 3667 4428
Size: 275 186 49 103 28 6 1480 761
Cuts at: 4428 5456 1540
Size: 1028 2301
Fragments arranged by size:
2301 1480 1028 761 275 186 103 49
28 6
BsiHKAI G_wGCw'C
Cuts at: 2384 2489 4278 4363 5434 2384
Size: 105 1789 85 1071 3167
Fragments arranged by size:
3167 1789 1071 105 85
BsmI GAATG_Cn'
Cuts at: 3045 3728 3821 3045
Size: 683 93 5441
Fragments arranged by size:
5441 683 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2384 2489 2496 3536 4278 4363 5434
Size: 399 594 105 7 1040 742 85 1071
Cuts at: 5434 1391
Size: 2174
Fragments arranged by size:
2174 1071 1040 742 594 399 105 85
7
BspEI T'CCGG_A
Cuts at: 5880 5880
Size: 6217
BspHI T'CATG_A
Cuts at: 4106 5836 4106
Size: 1730 4487
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2119 3572 844
Size: 1275 1453 3489
Fragments arranged by size:
3489 1453 1275
BsrBI CCG'CTC
Cuts at: 1394 1823 2011 2250 4104 1394
Size: 429 188 239 1854 3507
Fragments arranged by size:
3507 1854 429 239 188
BsrDI GCAATG_nn'
Cuts at: 65 1248 4712 4886 65
Size: 1183 3464 174 1396
Fragments arranged by size:
3464 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2110 2230 3033 4861 5048 1700
Size: 110 300 120 803 1828 187 2869
Fragments arranged by size:
2869 1828 803 300 187 120 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6217
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6217
BssSI C'ACGA_G
Cuts at: 4277 5289 4277
Size: 1012 5205
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6217
BstBI TT'CG_AA
Cuts at: 2634 5021 2634
Size: 2387 3830
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6217
BstYI r'GATC_y
Cuts at: 1972 2986 3547 3914 4299 4316 5768 5854 5866
Size: 1014 561 367 385 17 1452 86 12
Cuts at: 5866 6212 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1014 561 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2658 2658
Size: 6217
BtgI C'CryG_G
Cuts at: 513 1065 2004 2181 2279 2311 2556 513
Size: 552 939 177 98 32 245 4174
Fragments arranged by size:
4174 939 552 245 177 98 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5478
Fragments arranged by size:
5478 683 56
BtsI GCAGTG_nn'
Cuts at: 3733 4527 4547 3733
Size: 794 20 5403
Fragments arranged by size:
5403 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6217
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6182
DraI TTT'AAA
Cuts at: 3096 3521 3096
Size: 425 5792
DraIII CAC_nnn'GTG
Cuts at: 2021 2021
Size: 6217
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5224 809
Size: 507 3908 1802
Fragments arranged by size:
3908 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2047 2150 2178
Size: 54 1425 50 275 18 217 103 28
Cuts at: 2178 2184 2412 3404 3664 4553 6182 8
Size: 6 228 992 260 889 1629 43
Fragments arranged by size:
1629 1425 992 889 275 260 228 217
103 54 50 43 28 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 2047 2150 2178 2184 3664 1537
Size: 275 235 103 28 6 1480 4090
Fragments arranged by size:
4090 1480 275 235 103 28 6
EarI CTCTTCn'nnn_
Cuts at: 1353 2865 3243 3334 4147 1353
Size: 1512 378 91 813 3423
Fragments arranged by size:
3423 1512 813 378 91
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2079 4804 5178 5324 387
Size: 1064 138 490 2725 374 146 1280
Fragments arranged by size:
2725 1280 1064 490 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2264 2871 3478 4280 4877 5664 5932
Size: 393 632 607 607 802 597 787 268
Cuts at: 5932 1239
Size: 1524
Fragments arranged by size:
1524 802 787 632 607 607 597 393
268
EcoNI CCTnn'n_nnAGG
Cuts at: 3297 3297
Size: 6217
EcoO109I rG'GnC_Cy
Cuts at: 2883 2883
Size: 6217
EcoRI G'AATT_C
Cuts at: 3526 3904 3526
Size: 378 5839
EcoRV GAT'ATC
Cuts at: 2998 2998
Size: 6217
FspI TGC'GCA
Cuts at: 2432 4723 2432
Size: 2291 3926
HaeII r_GCGC'y
Cuts at: 1678 2330 2347 2401 2429 5364 1678
Size: 652 17 54 28 2935 2531
Fragments arranged by size:
2935 2531 652 54 28 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2891 2923 3288 3320 4858
Size: 32 135 32 1319 32 365 32 1538
Cuts at: 4858 4890 4932 4964 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1538 1319 365 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3561 3807 669
Size: 988 281 1623 246 3079
Fragments arranged by size:
3079 1623 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6217
HpaI GTT'AAC
Cuts at: 3807 3807
Size: 6217
KpnI G_GTAC'C
Cuts at: 2117 3542 2117
Size: 1425 4792
MfeI C'AATT_G
Cuts at: 3816 3816
Size: 6217
MluI A'CGCG_T
Cuts at: 6093 6093
Size: 6217
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2841 2987 5331 5515 1305
Size: 1536 146 2344 184 2007
Fragments arranged by size:
2344 2007 1536 184 146
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4395
Fragments arranged by size:
4395 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4175 4534 4693 518
Size: 648 3009 359 159 2042
Fragments arranged by size:
3009 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2177 2183 2198 2281 2301 2355 3633 4310
Size: 571 6 15 83 20 54 1278 677
Cuts at: 4310 5458 5703 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1278 1148 677 571 245 83 54
20 15 6
NaeI GCC'GGC
Cuts at: 2232 3035 2232
Size: 803 5414
NcoI C'CATG_G
Cuts at: 513 1065 2004 2311 513
Size: 552 939 307 4419
Fragments arranged by size:
4419 939 552 307
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6217
NgoMIV G'CCGG_C
Cuts at: 2230 3033 2230
Size: 803 5414
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6217
NotI GC'GGCC_GC
Cuts at: 2150 2178 2184 3664 2150
Size: 28 6 1480 4703
Fragments arranged by size:
4703 1480 28 6
NspI r_CATG'y
Cuts at: 1132 1229 3575 5120 1132
Size: 97 2346 1545 2229
Fragments arranged by size:
2346 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2066 1348
Size: 718 5499
PciI A'CATG_T
Cuts at: 5116 5116
Size: 6217
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2317 1108
Size: 1209 5008
PfoI T'CCnGG_A
Cuts at: 2848 2848
Size: 6217
PmeI GTTT'AAAC
Cuts at: 3521 3521
Size: 6217
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4286 4318 5799 5831 4286
Size: 32 1481 32 4672
Fragments arranged by size:
4672 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2883 2883
Size: 6217
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6217
PsiI TTA'TAA
Cuts at: 3787 3787
Size: 6217
PstI C_TGCA'G
Cuts at: 830 3337 3569 830
Size: 2507 232 3478
Fragments arranged by size:
3478 2507 232
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6217
SacII CC_GC'GG
Cuts at: 2184 2282 2184
Size: 98 6119
SalI G'TCGA_C
Cuts at: 1936 3559 1936
Size: 1623 4594
SbfI CC_TGCA'GG
Cuts at: 3569 3569
Size: 6217
ScaI AGT'ACT
Cuts at: 4465 6177 4465
Size: 1712 4505
SfcI C'TryA_G
Cuts at: 826 1045 2471 3333 3565 4700 5381 5572 826
Size: 219 1426 862 232 1135 681 191 1471
Fragments arranged by size:
1471 1426 1135 862 681 232 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6217
SmaI CCC'GGG
Cuts at: 1872 2239 2465 3544 1872
Size: 367 226 1079 4545
Fragments arranged by size:
4545 1079 367 226
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2330 2599 2974 4321 5222 5484
Size: 197 926 387 269 375 1347 901 262
Cuts at: 5484 820
Size: 1553
Fragments arranged by size:
1553 1347 926 901 387 375 269 262
197
SnaBI TAC'GTA
Cuts at: 493 4008 493
Size: 3515 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6217
SphI G_CATG'C
Cuts at: 1132 3575 1132
Size: 2443 3774
SspI AAT'ATT
Cuts at: 5 52 4141 5
Size: 47 4089 2081
Fragments arranged by size:
4089 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6217
StyI C'CwwG_G
Cuts at: 513 1065 2004 2311 2746 2949 3656 513
Size: 552 939 307 435 203 707 3074
Fragments arranged by size:
3074 939 707 552 435 307 203
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2049 2582 4244 4261 4414 4599 5044
Size: 1255 469 533 1662 17 153 185 445
Cuts at: 5044 325
Size: 1498
Fragments arranged by size:
1662 1498 1255 533 469 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3489 4463 6175
Size: 274 80 33 51 1443 1512 974 1712
Cuts at: 6175 96
Size: 138
Fragments arranged by size:
1712 1512 1443 974 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2354 2386 6177 6209 2354
Size: 32 3791 32 2362
Fragments arranged by size:
3791 2362 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2968 1316
Size: 1652 4565
XbaI T'CTAG_A
Cuts at: 3553 3553
Size: 6217
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6217
XmaI C'CCGG_G
Cuts at: 1870 2237 2463 3542 1870
Size: 367 226 1079 4545
Fragments arranged by size:
4545 1079 367 226
XmnI GAAnn'nnTTC
Cuts at: 3045 4346 3045
Size: 1301 4916
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4025 276
Size: 53 83 186 936 2491 2468
Fragments arranged by size:
2491 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI FalI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI