Restriction map for
pFN21AB7049
(Circular) (Six-base) MAPSORT of: pFN21AB7049.seq Check: 2332 from: 1 to: 6181
pFN21AB7049 6181 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3991 278
Size: 53 83 186 936 2455 2468
Fragments arranged by size:
2468 2455 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3524 1937
Size: 1587 4594
Acc65I G'GTAC_C
Cuts at: 3502 3502
Size: 6181
AclI AA'CG_TT
Cuts at: 1563 4308 4681 1563
Size: 2745 373 3063
Fragments arranged by size:
3063 2745 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2072 3351 3420 3450 4244 5628 5896 1632
Size: 440 1279 69 30 794 1384 268 1917
Fragments arranged by size:
1917 1384 1279 794 440 268 69 30
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5984
AflIII A'CryG_T
Cuts at: 1203 5080 6057 1203
Size: 3877 977 1327
Fragments arranged by size:
3877 1327 977
AgeI A'CCGG_T
Cuts at: 5012 5012
Size: 6181
AhdI GACnn_n'nnGTC
Cuts at: 2815 3465 4910 2815
Size: 650 1445 4086
Fragments arranged by size:
4086 1445 650
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6181
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2838 1184
Size: 1654 4527
AlwNI CAG_nnn'CTG
Cuts at: 3924 5496 3924
Size: 1572 4609
ApaLI G'TGCA_C
Cuts at: 4238 5394 4238
Size: 1156 5025
ApoI r'AATT_y
Cuts at: 1493 3254 3490 3716 3868 5977 1493
Size: 1761 236 226 152 2109 1697
Fragments arranged by size:
2109 1761 1697 236 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4735 160
Size: 872 3703 1606
Fragments arranged by size:
3703 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6181
AvaI C'yCGr_G
Cuts at: 1870 1943 3282 3506 1870
Size: 73 1339 224 4545
Fragments arranged by size:
4545 1339 224 73
BamHI G'GATC_C
Cuts at: 2231 3418 3511 3878 2231
Size: 1187 93 367 4534
Fragments arranged by size:
4534 1187 367 93
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2020 3502 4957 618
Size: 325 217 627 233 1482 1455 1842
Fragments arranged by size:
1842 1482 1455 627 325 233 217
BanII G_rGCy'C
Cuts at: 1391 3018 3083 3500 1391
Size: 1627 65 417 4072
Fragments arranged by size:
4072 1627 417 65
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5210
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4372 4406 1903
Size: 34 50 34 2351 34 3678
Fragments arranged by size:
3678 2351 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4073 5283 4073
Size: 1210 4971
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6181
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4792 136
Size: 107 122 71 631 605 3120 1525
Fragments arranged by size:
3120 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3395 6176 3395
Size: 2781 3400
BlpI GC'TnA_GC
Cuts at: 3598 3598
Size: 6181
Bme1580I G_kGCm'C
Cuts at: 1790 2025 2513 4242 5398 1790
Size: 235 488 1729 1156 2573
Fragments arranged by size:
2573 1729 1156 488 235
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6181
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2036 2726 2955
Size: 416 53 83 399 24 619 690 229
Cuts at: 2955 3163 3912 4870 442
Size: 208 749 958 1753
Fragments arranged by size:
1753 958 749 690 619 416 399 229
208 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4841 1239
Size: 3602 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2627 4306 5171 5469 2627
Size: 1679 865 298 3339
Fragments arranged by size:
3339 1679 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3473 4844 882
Size: 617 1974 1371 2219
Fragments arranged by size:
2219 1974 1371 617
BsaAI yAC'GTr
Cuts at: 493 1204 2465 3972 493
Size: 711 1261 1507 2702
Fragments arranged by size:
2702 1507 1261 711
BsaBI GATnn'nnATC
Cuts at: 2604 6175 2604
Size: 3571 2610
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3988 4370 5005 275
Size: 53 83 186 936 2455 382 635 1451
Fragments arranged by size:
2455 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3464 4614 5012 5286 5433 5844 3464
Size: 1150 398 274 147 411 3801
Fragments arranged by size:
3801 1150 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2768 2798 1178
Size: 30 165 30 1365 30 4561
Fragments arranged by size:
4561 1365 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3461 1186
Size: 2275 3906
BseYI C'CCAG_C
Cuts at: 3443 3477 5384 5880 3443
Size: 34 1907 496 3744
Fragments arranged by size:
3744 1907 496 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2499 1163
Size: 573 763 4845
Fragments arranged by size:
4845 763 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3631 4392 5420 1540
Size: 275 186 1630 761 1028 2301
Fragments arranged by size:
2301 1630 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3018 4242 4327 5398 3018
Size: 1224 85 1071 3801
Fragments arranged by size:
3801 1224 1071 85
BsmI GAATG_Cn'
Cuts at: 3692 3785 3692
Size: 93 6088
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2513 3018 3083 3500 4242 4327
Size: 399 235 488 505 65 417 742 85
Cuts at: 4327 5398 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 505 488 417 399 235
85 65
BspEI T'CCGG_A
Cuts at: 5844 5844
Size: 6181
BspHI T'CATG_A
Cuts at: 2596 3010 4070 5800 2596
Size: 414 1060 1730 2977
Fragments arranged by size:
2977 1730 1060 414
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3536 844
Size: 2692 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4068 1394
Size: 429 2245 3507
Fragments arranged by size:
3507 2245 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3240 4676 4850 65
Size: 1183 1992 1436 174 1396
Fragments arranged by size:
1992 1436 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4825 5012 1700
Size: 110 3015 187 2869
Fragments arranged by size:
3015 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2503 2566 96
Size: 2407 63 3711
Fragments arranged by size:
3711 2407 63
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6181
BssSI C'ACGA_G
Cuts at: 4241 5253 4241
Size: 1012 5169
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6181
BstBI TT'CG_AA
Cuts at: 4985 4985
Size: 6181
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6181
BstYI r'GATC_y
Cuts at: 1972 2231 3395 3418 3511 3878 4263 4280 5732
Size: 259 1164 23 93 367 385 17 1452
Cuts at: 5732 5818 5830 6176 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1164 385 367 346 259 93
86 23 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5629
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5442
Fragments arranged by size:
5442 683 56
BtsI GCAGTG_nn'
Cuts at: 3697 4491 4511 3697
Size: 794 20 5367
Fragments arranged by size:
5367 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6181
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6146
DraI TTT'AAA
Cuts at: 3485 3485
Size: 6181
DraIII CAC_nnn'GTG
Cuts at: 2963 2963
Size: 6181
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2086 5188 809
Size: 507 770 3102 1802
Fragments arranged by size:
3102 1802 770 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2888 3029 3067
Size: 54 1425 50 275 18 1058 141 38
Cuts at: 3067 3628 4517 6146 8
Size: 561 889 1629 43
Fragments arranged by size:
1629 1425 1058 889 561 275 141 54
50 43 38 18
EagI C'GGCC_G
Cuts at: 1537 1812 3628 1537
Size: 275 1816 4090
Fragments arranged by size:
4090 1816 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2041 2571 3309 4111 1353
Size: 688 530 738 802 3423
Fragments arranged by size:
3423 802 738 688 530
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2644 4768 5142 5288 387
Size: 1064 138 1055 2124 374 146 1280
Fragments arranged by size:
2124 1280 1064 1055 374 146 138
EcoICRI GAG'CTC
Cuts at: 3016 3016
Size: 6181
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 3351 3420 3450 4244 4841 5628
Size: 393 440 1279 69 30 794 597 787
Cuts at: 5628 5896 1239
Size: 268 1524
Fragments arranged by size:
1524 1279 794 787 597 440 393 268
69 30
EcoO109I rG'GnC_Cy
Cuts at: 2072 2072
Size: 6181
EcoRI G'AATT_C
Cuts at: 3490 3868 3490
Size: 378 5803
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2077 2077
Size: 6181
FspI TGC'GCA
Cuts at: 4687 4687
Size: 6181
HaeII r_GCGC'y
Cuts at: 1678 5328 1678
Size: 3650 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2483 2515 2768 2800 2801
Size: 32 135 32 911 32 253 32 1
Cuts at: 2801 2833 3451 3483 4822 4854 4896 4928 1373
Size: 32 618 32 1339 32 42 32 2626
Fragments arranged by size:
2626 1339 911 618 253 135 42 32
32 32 32 32 32 32 32 1
HincII GTy'rAC
Cuts at: 669 1657 1938 3525 3771 669
Size: 988 281 1587 246 3079
Fragments arranged by size:
3079 1587 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3432 748
Size: 2684 3497
HpaI GTT'AAC
Cuts at: 3771 3771
Size: 6181
KpnI G_GTAC'C
Cuts at: 3506 3506
Size: 6181
MfeI C'AATT_G
Cuts at: 3780 3780
Size: 6181
MluI A'CGCG_T
Cuts at: 6057 6057
Size: 6181
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5295 5479 1305
Size: 3990 184 2007
Fragments arranged by size:
3990 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3031 3069 10
Size: 54 1425 343 1199 38 3122
Fragments arranged by size:
3122 1425 1199 343 54 38
MslI CAynn'nnrTG
Cuts at: 518 1166 3179 4139 4498 4657 518
Size: 648 2013 960 359 159 2042
Fragments arranged by size:
2042 2013 960 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3597 4274 5422 5667 1606
Size: 1991 677 1148 245 2120
Fragments arranged by size:
2120 1991 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5629
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6181
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6181
NotI GC'GGCC_GC
Cuts at: 3628 3628
Size: 6181
NspI r_CATG'y
Cuts at: 1132 1229 2641 2793 2815 3539 5084 1132
Size: 97 1412 152 22 724 1545 2229
Fragments arranged by size:
2229 1545 1412 724 152 97 22
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6181
PciI A'CATG_T
Cuts at: 5080 5080
Size: 6181
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2151 2917 1108
Size: 1043 766 4372
Fragments arranged by size:
4372 1043 766
PmeI GTTT'AAAC
Cuts at: 3485 3485
Size: 6181
PmlI CAC'GTG
Cuts at: 2465 2465
Size: 6181
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2825 2857 4250 4282 5763 5795 2825
Size: 32 1393 32 1481 32 3211
Fragments arranged by size:
3211 1481 1393 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6181
PsiI TTA'TAA
Cuts at: 3751 3751
Size: 6181
PstI C_TGCA'G
Cuts at: 830 3533 830
Size: 2703 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6181
SacI G_AGCT'C
Cuts at: 3018 3018
Size: 6181
SalI G'TCGA_C
Cuts at: 1936 3523 1936
Size: 1587 4594
SapI GCTCTTCn'nnn_
Cuts at: 3309 3309
Size: 6181
SbfI CC_TGCA'GG
Cuts at: 3533 3533
Size: 6181
ScaI AGT'ACT
Cuts at: 2408 4429 6141 2408
Size: 2021 1712 2448
Fragments arranged by size:
2448 2021 1712
SexAI A'CCwGG_T
Cuts at: 3000 3000
Size: 6181
SfcI C'TryA_G
Cuts at: 826 1045 2185 3529 4664 5345 5536 826
Size: 219 1140 1344 1135 681 191 1471
Fragments arranged by size:
1471 1344 1140 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6181
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6181
SmaI CCC'GGG
Cuts at: 1872 3284 3508 1872
Size: 1412 224 4545
Fragments arranged by size:
4545 1412 224
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2642 4285 5186 5448 820
Size: 197 926 699 1643 901 262 1553
Fragments arranged by size:
1643 1553 926 901 699 262 197
SnaBI TAC'GTA
Cuts at: 493 3972 493
Size: 3479 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6181
SphI G_CATG'C
Cuts at: 1132 2641 3539 1132
Size: 1509 898 3774
Fragments arranged by size:
3774 1509 898
SspI AAT'ATT
Cuts at: 5 52 4105 5
Size: 47 4053 2081
Fragments arranged by size:
4053 2081 47
StuI AGG'CCT
Cuts at: 1517 2748 3165 1517
Size: 1231 417 4533
Fragments arranged by size:
4533 1231 417
StyI C'CwwG_G
Cuts at: 513 1065 3620 513
Size: 552 2555 3074
Fragments arranged by size:
3074 2555 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2974 4208 4225 4378 4563 5008 325
Size: 1255 1394 1234 17 153 185 445 1498
Fragments arranged by size:
1498 1394 1255 1234 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2406 2503 2566
Size: 274 80 33 51 1443 429 97 63
Cuts at: 2566 4427 6139 96
Size: 1861 1712 138
Fragments arranged by size:
1861 1712 1443 429 274 138 97 80
63 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6141 6173 6141
Size: 32 6149
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6181
XbaI T'CTAG_A
Cuts at: 3517 3517
Size: 6181
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6181
XmaI C'CCGG_G
Cuts at: 1870 3282 3506 1870
Size: 1412 224 4545
Fragments arranged by size:
4545 1412 224
XmnI GAAnn'nnTTC
Cuts at: 2337 4310 2337
Size: 1973 4208
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3989 276
Size: 53 83 186 936 2455 2468
Fragments arranged by size:
2468 2455 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI
SacI SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacII SanDI SfiI SfoI SrfI
SwaI XcmI