Restriction map for
pFN21AB7045
(Circular) (Six-base) MAPSORT of: pFN21AB7045.seq Check: 2190 from: 1 to: 6133
pFN21AB7045 6133 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2955 2955
Size: 6133
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3943 278
Size: 53 83 186 936 2407 2468
Fragments arranged by size:
2468 2407 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2025 3476 1937
Size: 88 1451 4594
Fragments arranged by size:
4594 1451 88
Acc65I G'GTAC_C
Cuts at: 3356 3454 3356
Size: 98 6035
AclI AA'CG_TT
Cuts at: 1563 4260 4633 1563
Size: 2697 373 3063
Fragments arranged by size:
3063 2697 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3124 4196 5580 5848 1632
Size: 1492 1072 1384 268 1917
Fragments arranged by size:
1917 1492 1384 1072 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5936
AflIII A'CryG_T
Cuts at: 1203 5032 6009 1203
Size: 3829 977 1327
Fragments arranged by size:
3829 1327 977
AgeI A'CCGG_T
Cuts at: 3182 4964 3182
Size: 1782 4351
AhdI GACnn_n'nnGTC
Cuts at: 4862 4862
Size: 6133
AleI CACnn'nnGTG
Cuts at: 1166 2108 3183 1166
Size: 942 1075 4116
Fragments arranged by size:
4116 1075 942
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3058 1184
Size: 1874 4259
AlwNI CAG_nnn'CTG
Cuts at: 3876 5448 3876
Size: 1572 4561
ApaI G_GGCC'C
Cuts at: 2831 2831
Size: 6133
ApaLI G'TGCA_C
Cuts at: 2057 2458 3008 4190 5346 2057
Size: 401 550 1182 1156 2844
Fragments arranged by size:
2844 1182 1156 550 401
ApoI r'AATT_y
Cuts at: 1493 3442 3668 3820 5929 1493
Size: 1949 226 152 2109 1697
Fragments arranged by size:
2109 1949 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4687 160
Size: 872 3655 1606
Fragments arranged by size:
3655 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6133
AvaI C'yCGr_G
Cuts at: 1870 1943 3458 1870
Size: 73 1515 4545
Fragments arranged by size:
4545 1515 73
BamHI G'GATC_C
Cuts at: 3463 3830 3463
Size: 367 5766
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2315 2776 3307 3356 3454
Size: 325 217 627 528 461 531 49 98
Cuts at: 3454 4909 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 531 528 461 325 217
98 49
BanII G_rGCy'C
Cuts at: 1391 2831 3452 1391
Size: 1440 621 4072
Fragments arranged by size:
4072 1440 621
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5162
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4324 4358 1903
Size: 34 2387 34 3678
Fragments arranged by size:
3678 2387 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4025 5235 4025
Size: 1210 4923
BclI T'GATC_A
Cuts at: 1548 2181 1548
Size: 633 5500
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2956 4744 136
Size: 107 122 71 631 605 1284 1788 1525
Fragments arranged by size:
1788 1525 1284 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6128 6128
Size: 6133
BlpI GC'TnA_GC
Cuts at: 2899 3550 2899
Size: 651 5482
Bme1580I G_kGCm'C
Cuts at: 1790 2061 2462 2781 2831 3012 3032 3295 4194
Size: 271 401 319 50 181 20 263 899
Cuts at: 4194 5350 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 899 401 319 271 263 181
50 20
BmgBI CAC'GTC
Cuts at: 1318 2106 1318
Size: 788 5345
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2030 2144 2826
Size: 416 53 83 399 24 613 114 682
Cuts at: 2826 3108 3864 4822 442
Size: 282 756 958 1753
Fragments arranged by size:
1753 958 756 682 613 416 399 282
114 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6133
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3416 3416
Size: 6133
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2220 4793 1239
Size: 981 2573 2579
Fragments arranged by size:
2579 2573 981
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4258 5123 5421 4258
Size: 865 298 4970
Fragments arranged by size:
4970 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2535 2663 2706 4796 882
Size: 617 1036 128 43 2090 2219
Fragments arranged by size:
2219 2090 1036 617 128 43
BsaAI yAC'GTr
Cuts at: 493 1204 3924 493
Size: 711 2720 2702
Fragments arranged by size:
2720 2702 711
BsaBI GATnn'nnATC
Cuts at: 6127 6127
Size: 6133
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3002 3940 4322 4957
Size: 53 83 186 936 1469 938 382 635
Cuts at: 4957 275
Size: 1451
Fragments arranged by size:
1469 1451 938 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2846 3182 4566 4964 5238 5385 5796 2846
Size: 336 1384 398 274 147 411 3183
Fragments arranged by size:
3183 1384 411 398 336 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5908
Fragments arranged by size:
5908 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2505 2508 3403 1186
Size: 1319 3 895 3916
Fragments arranged by size:
3916 1319 895 3
BseYI C'CCAG_C
Cuts at: 2231 2383 2475 2837 2993 3031 5336 5832 2231
Size: 152 92 362 156 38 2305 496 2532
Fragments arranged by size:
2532 2305 496 362 156 152 92 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2238 2376 2556 3365 3385 1163
Size: 573 502 138 180 809 20 3911
Fragments arranged by size:
3911 809 573 502 180 138 20
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3049 3583 4344 5372 1540
Size: 275 186 1048 534 761 1028 2301
Fragments arranged by size:
2301 1048 1028 761 534 275 186
BsiHKAI G_wGCw'C
Cuts at: 2061 2462 3012 4194 4279 5350 2061
Size: 401 550 1182 85 1071 2844
Fragments arranged by size:
2844 1182 1071 550 401 85
BsmI GAATG_Cn'
Cuts at: 3644 3737 3644
Size: 93 6040
BsmBI CGTCTCn'nnnn_
Cuts at: 2254 2254
Size: 6133
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2061 2462 2781 2831 3012 3032 3295
Size: 399 271 401 319 50 181 20 263
Cuts at: 3295 3452 4194 4279 5350 1391
Size: 157 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 401 399 319 271 263
181 157 85 50 20
BspEI T'CCGG_A
Cuts at: 5796 5796
Size: 6133
BspHI T'CATG_A
Cuts at: 4022 5752 4022
Size: 1730 4403
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2955 3488 844
Size: 2111 533 3489
Fragments arranged by size:
3489 2111 533
BsrBI CCG'CTC
Cuts at: 1394 1823 3063 3280 3400 4020 1394
Size: 429 1240 217 120 620 3507
Fragments arranged by size:
3507 1240 620 429 217 120
BsrDI GCAATG_nn'
Cuts at: 65 1248 4628 4802 65
Size: 1183 3380 174 1396
Fragments arranged by size:
3380 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3004 3048 3182 4777 4964 1700
Size: 110 1194 44 134 1595 187 2869
Fragments arranged by size:
2869 1595 1194 187 134 110 44
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6133
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6133
BssSI C'ACGA_G
Cuts at: 3011 4193 5205 3011
Size: 1182 1012 3939
Fragments arranged by size:
3939 1182 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6133
BstBI TT'CG_AA
Cuts at: 4937 4937
Size: 6133
BstEII G'GTnAC_C
Cuts at: 1184 2110 2842 1184
Size: 926 732 4475
Fragments arranged by size:
4475 926 732
BstXI CCAn_nnnn'nTGG
Cuts at: 2434 2434
Size: 6133
BstYI r'GATC_y
Cuts at: 1972 2218 3123 3258 3463 3830 4215 4232 5684
Size: 246 905 135 205 367 385 17 1452
Cuts at: 5684 5770 5782 6128 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 905 385 367 346 246 205
135 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2646 513
Size: 552 1581 4000
Fragments arranged by size:
4000 1581 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5394
Fragments arranged by size:
5394 683 56
BtsI GCAGTG_nn'
Cuts at: 2951 3154 3295 3649 4443 4463 2951
Size: 203 141 354 794 20 4621
Fragments arranged by size:
4621 794 354 203 141 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6133
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6098
DraI TTT'AAA
Cuts at: 3437 3437
Size: 6133
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5140 809
Size: 507 3824 1802
Fragments arranged by size:
3824 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2166 2436 3214
Size: 54 1425 50 275 18 336 270 778
Cuts at: 3214 3580 4469 6098 8
Size: 366 889 1629 43
Fragments arranged by size:
1629 1425 889 778 366 336 275 270
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3580 1537
Size: 275 1768 4090
Fragments arranged by size:
4090 1768 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2201 2599 2666 4063 1353
Size: 848 398 67 1397 3423
Fragments arranged by size:
3423 1397 848 398 67
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2493 3405 4720 5094 5240 387
Size: 1064 138 904 912 1315 374 146 1280
Fragments arranged by size:
1315 1280 1064 912 904 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2220 3124 4196 4793 5580 5848 1239
Size: 393 588 904 1072 597 787 268 1524
Fragments arranged by size:
1524 1072 904 787 597 588 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2549 2549
Size: 6133
EcoO109I rG'GnC_Cy
Cuts at: 2092 2141 2444 2554 2827 2828 2876 3018 2092
Size: 49 303 110 273 1 48 142 5207
Fragments arranged by size:
5207 303 273 142 110 49 48 1
EcoRI G'AATT_C
Cuts at: 3442 3820 3442
Size: 378 5755
FspI TGC'GCA
Cuts at: 3134 4639 3134
Size: 1505 4628
HaeII r_GCGC'y
Cuts at: 1678 3250 5280 1678
Size: 1572 2030 2531
Fragments arranged by size:
2531 2030 1572
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2195 2227 4774 4806 4848
Size: 32 135 32 623 32 2547 32 42
Cuts at: 4848 4880 1373
Size: 32 2626
Fragments arranged by size:
2626 2547 623 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3477 3723 669
Size: 988 281 1539 246 3079
Fragments arranged by size:
3079 1539 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6133
HpaI GTT'AAC
Cuts at: 3723 3723
Size: 6133
KpnI G_GTAC'C
Cuts at: 3360 3458 3360
Size: 98 6035
MfeI C'AATT_G
Cuts at: 3732 3732
Size: 6133
MluI A'CGCG_T
Cuts at: 6009 6009
Size: 6133
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2196 3101 5247 5431 1305
Size: 891 905 2146 184 2007
Fragments arranged by size:
2146 2007 905 891 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2438 10
Size: 54 1425 343 606 3705
Fragments arranged by size:
3705 1425 606 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2108 2192 2279 2701 3183 4091 4450
Size: 648 942 84 87 422 482 908 359
Cuts at: 4450 4609 518
Size: 159 2042
Fragments arranged by size:
2042 942 908 648 482 422 359 159
87 84
MspA1I CmG'CkG
Cuts at: 1606 2065 2322 2729 3549 4226 5374 5619 1606
Size: 459 257 407 820 677 1148 245 2120
Fragments arranged by size:
2120 1148 820 677 459 407 257 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5581
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6133
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6133
NotI GC'GGCC_GC
Cuts at: 3580 3580
Size: 6133
NspI r_CATG'y
Cuts at: 1132 1229 3133 3491 5036 1132
Size: 97 1904 358 1545 2229
Fragments arranged by size:
2229 1904 1545 358 97
PasI CC'CwG_GG
Cuts at: 1348 2449 2821 1348
Size: 1101 372 4660
Fragments arranged by size:
4660 1101 372
PciI A'CATG_T
Cuts at: 5032 5032
Size: 6133
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6133
PfoI T'CCnGG_A
Cuts at: 2683 2683
Size: 6133
PmeI GTTT'AAAC
Cuts at: 3437 3437
Size: 6133
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4202 4234 5715 5747 4202
Size: 32 1481 32 4588
Fragments arranged by size:
4588 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2092 2444 2554 2876 2092
Size: 352 110 322 5349
Fragments arranged by size:
5349 352 322 110
PshAI GACnn'nnGTC
Cuts at: 1613 2655 1613
Size: 1042 5091
PsiI TTA'TAA
Cuts at: 3703 3703
Size: 6133
PspOMI G'GGCC_C
Cuts at: 2827 2827
Size: 6133
PstI C_TGCA'G
Cuts at: 830 3485 830
Size: 2655 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6133
PvuII CAG'CTG
Cuts at: 2065 2322 2729 2065
Size: 257 407 5469
Fragments arranged by size:
5469 407 257
SalI G'TCGA_C
Cuts at: 1936 3475 1936
Size: 1539 4594
SanDI GG'GwC_CC
Cuts at: 2876 2876
Size: 6133
SbfI CC_TGCA'GG
Cuts at: 3485 3485
Size: 6133
ScaI AGT'ACT
Cuts at: 4381 6093 4381
Size: 1712 4421
SfcI C'TryA_G
Cuts at: 826 1045 3321 3481 4616 5297 5488 826
Size: 219 2276 160 1135 681 191 1471
Fragments arranged by size:
2276 1471 1135 681 219 191 160
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6133
SgrAI Cr'CCGG_yG
Cuts at: 1700 3004 3182 1700
Size: 1304 178 4651
Fragments arranged by size:
4651 1304 178
SmaI CCC'GGG
Cuts at: 1872 3460 1872
Size: 1588 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4237 5138 5400 820
Size: 197 926 2294 901 262 1553
Fragments arranged by size:
2294 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3924 493
Size: 3431 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6133
SphI G_CATG'C
Cuts at: 1132 3133 3491 1132
Size: 2001 358 3774
Fragments arranged by size:
3774 2001 358
SspI AAT'ATT
Cuts at: 5 52 2347 4057 5
Size: 47 2295 1710 2081
Fragments arranged by size:
2295 2081 1710 47
StuI AGG'CCT
Cuts at: 1517 2971 1517
Size: 1454 4679
StyI C'CwwG_G
Cuts at: 513 1065 2016 3572 513
Size: 552 951 1556 3074
Fragments arranged by size:
3074 1556 951 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2441 3179 3191 4160 4177 4330 4515
Size: 1255 861 738 12 969 17 153 185
Cuts at: 4515 4960 325
Size: 445 1498
Fragments arranged by size:
1498 1255 969 861 738 445 185 153
17 12
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2496 4379 6091
Size: 274 80 33 51 1443 519 1883 1712
Cuts at: 6091 96
Size: 138
Fragments arranged by size:
1883 1712 1443 519 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3069 3101 3274 3306 6093 6125 3069
Size: 32 173 32 2787 32 3077
Fragments arranged by size:
3077 2787 173 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6133
XbaI T'CTAG_A
Cuts at: 3469 3469
Size: 6133
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2131 2446 2131
Size: 315 5818
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6133
XmaI C'CCGG_G
Cuts at: 1870 3458 1870
Size: 1588 4545
XmnI GAAnn'nnTTC
Cuts at: 3194 4262 3194
Size: 1068 5065
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3941 276
Size: 53 83 186 936 2407 2468
Fragments arranged by size:
2468 2407 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI RsrII SacI SacII SapI
SexAI SfiI SfoI SrfI SwaI