Restriction map for
pFN21AB7043
(Circular) (Six-base) MAPSORT of: pFN21AB7043.seq Check: 1417 from: 1 to: 6115
pFN21AB7043 6115 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3925 278
Size: 53 83 186 936 2389 2468
Fragments arranged by size:
2468 2389 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3458 1937
Size: 1521 4594
Acc65I G'GTAC_C
Cuts at: 3436 3436
Size: 6115
AclI AA'CG_TT
Cuts at: 1563 4242 4615 1563
Size: 2679 373 3063
Fragments arranged by size:
3063 2679 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3334 4178 5562 5830 1632
Size: 1702 844 1384 268 1917
Fragments arranged by size:
1917 1702 1384 844 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5918
AflIII A'CryG_T
Cuts at: 1203 5014 5991 1203
Size: 3811 977 1327
Fragments arranged by size:
3811 1327 977
AgeI A'CCGG_T
Cuts at: 4946 4946
Size: 6115
AhdI GACnn_n'nnGTC
Cuts at: 2307 4844 2307
Size: 2537 3578
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6115
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6115
AlwNI CAG_nnn'CTG
Cuts at: 2836 2854 3858 5430 2836
Size: 18 1004 1572 3521
Fragments arranged by size:
3521 1572 1004 18
ApaI G_GGCC'C
Cuts at: 2704 3270 2704
Size: 566 5549
ApaLI G'TGCA_C
Cuts at: 4172 5328 4172
Size: 1156 4959
ApoI r'AATT_y
Cuts at: 1493 3424 3650 3802 5911 1493
Size: 1931 226 152 2109 1697
Fragments arranged by size:
2109 1931 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4669 160
Size: 872 3637 1606
Fragments arranged by size:
3637 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6115
AvaI C'yCGr_G
Cuts at: 1870 1943 2144 2294 2342 2492 3440 1870
Size: 73 201 150 48 150 948 4545
Fragments arranged by size:
4545 948 201 150 150 73 48
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2375 2408 3109 3142 2375
Size: 33 701 33 5348
Fragments arranged by size:
5348 701 33 33
BamHI G'GATC_C
Cuts at: 3445 3812 3445
Size: 367 5748
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2281 2290 3034 3058 3436
Size: 325 217 627 494 9 744 24 378
Cuts at: 3436 4891 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 744 627 494 378 325 217
24 9
BanII G_rGCy'C
Cuts at: 1391 2145 2704 2946 3270 3434 1391
Size: 754 559 242 324 164 4072
Fragments arranged by size:
4072 754 559 324 242 164
BbeI G_GCGC'C
Cuts at: 2285 2294 2285
Size: 9 6106
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2421 2622 2801 928
Size: 971 522 201 179 4242
Fragments arranged by size:
4242 971 522 201 179
BbvCI CC'TCA_GC
Cuts at: 2779 2779
Size: 6115
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4306 4340 1903
Size: 34 2369 34 3678
Fragments arranged by size:
3678 2369 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4007 5217 4007
Size: 1210 4905
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6115
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4726 136
Size: 107 122 71 631 605 3054 1525
Fragments arranged by size:
3054 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6110 6110
Size: 6115
BlpI GC'TnA_GC
Cuts at: 3532 3532
Size: 6115
Bme1580I G_kGCm'C
Cuts at: 1790 2655 2704 3015 3063 3270 4176 5332 1790
Size: 865 49 311 48 207 906 1156 2573
Fragments arranged by size:
2573 1156 906 865 311 207 49 48
BmgBI CAC'GTC
Cuts at: 1318 3175 1318
Size: 1857 4258
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3846 4804 442
Size: 416 53 83 399 24 2429 958 1753
Fragments arranged by size:
2429 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6115
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2794 2794
Size: 6115
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2076 3130 3197 4775 1239
Size: 837 1054 67 1578 2579
Fragments arranged by size:
2579 1578 1054 837 67
Bpu10I CC'TnA_GC
Cuts at: 2779 2779
Size: 6115
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2732 2915 3131 4240 5105 5403 2732
Size: 183 216 1109 865 298 3444
Fragments arranged by size:
3444 1109 865 298 216 183
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2978 4778 882
Size: 617 1479 1800 2219
Fragments arranged by size:
2219 1800 1479 617
BsaAI yAC'GTr
Cuts at: 493 1204 2476 3906 493
Size: 711 1272 1430 2702
Fragments arranged by size:
2702 1430 1272 711
BsaBI GATnn'nnATC
Cuts at: 6109 6109
Size: 6115
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2282 2291 2879 3922
Size: 53 83 186 936 749 9 588 1043
Cuts at: 3922 4304 4939 275
Size: 382 635 1451
Fragments arranged by size:
1451 1043 936 749 635 588 382 186
83 53 9
BsaWI w'CCGG_w
Cuts at: 2683 4548 4946 5220 5367 5778 2683
Size: 1865 398 274 147 411 3020
Fragments arranged by size:
3020 1865 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5890
Fragments arranged by size:
5890 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2702 2710 1186
Size: 1516 8 4591
Fragments arranged by size:
4591 1516 8
BseYI C'CCAG_C
Cuts at: 2167 2305 2316 2874 2945 5318 5814 2167
Size: 138 11 558 71 2373 496 2468
Fragments arranged by size:
2468 2373 558 496 138 71 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5542
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2696 2738 3565 4326 5354 1540
Size: 275 186 695 42 827 761 1028 2301
Fragments arranged by size:
2301 1028 827 761 695 275 186 42
BsiHKAI G_wGCw'C
Cuts at: 2663 3162 4176 4261 5332 2663
Size: 499 1014 85 1071 3446
Fragments arranged by size:
3446 1071 1014 499 85
BsiWI C'GTAC_G
Cuts at: 2274 2274
Size: 6115
BsmI GAATG_Cn'
Cuts at: 3626 3719 3626
Size: 93 6022
BsmBI CGTCTCn'nnnn_
Cuts at: 2090 2090
Size: 6115
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2145 2655 2663 2704 2946 3015 3063
Size: 399 355 510 8 41 242 69 48
Cuts at: 3063 3162 3270 3434 4176 4261 5332 1391
Size: 99 108 164 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 510 399 355 242 164
108 99 85 69 48 41 8
BspEI T'CCGG_A
Cuts at: 2683 5778 2683
Size: 3095 3020
BspHI T'CATG_A
Cuts at: 4004 5734 4004
Size: 1730 4385
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3470 844
Size: 2626 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2357 2526 2692 4002 1394
Size: 429 534 169 166 1310 3507
Fragments arranged by size:
3507 1310 534 429 169 166
BsrDI GCAATG_nn'
Cuts at: 65 1248 3333 4610 4784 65
Size: 1183 2085 1277 174 1396
Fragments arranged by size:
2085 1396 1277 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2737 3040 4759 4946 1700
Size: 110 927 303 1719 187 2869
Fragments arranged by size:
2869 1719 927 303 187 110
BsrGI T'GTAC_A
Cuts at: 96 3404 96
Size: 3308 2807
BssHII G'CGCG_C
Cuts at: 1925 2074 1925
Size: 149 5966
BssSI C'ACGA_G
Cuts at: 2656 4175 5187 2656
Size: 1519 1012 3584
Fragments arranged by size:
3584 1519 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2857 794
Size: 2063 4052
BstBI TT'CG_AA
Cuts at: 4919 4919
Size: 6115
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6115
BstYI r'GATC_y
Cuts at: 1972 2961 3445 3812 4197 4214 5666 5752 5764
Size: 989 484 367 385 17 1452 86 12
Cuts at: 5764 6110 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 989 484 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2085 2085
Size: 6115
BtgI C'CryG_G
Cuts at: 513 1065 2004 2225 2379 2423 2580 513
Size: 552 939 221 154 44 157 4048
Fragments arranged by size:
4048 939 552 221 157 154 44
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2151 487
Size: 683 56 925 4451
Fragments arranged by size:
4451 925 683 56
BtsI GCAGTG_nn'
Cuts at: 3002 3194 3631 4425 4445 3002
Size: 192 437 794 20 4672
Fragments arranged by size:
4672 794 437 192 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6115
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6080
DraI TTT'AAA
Cuts at: 3419 3419
Size: 6115
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5122 809
Size: 507 3806 1802
Fragments arranged by size:
3806 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2501 2693 2735
Size: 54 1425 50 275 18 671 192 42
Cuts at: 2735 3562 4451 6080 8
Size: 827 889 1629 43
Fragments arranged by size:
1629 1425 889 827 671 275 192 54
50 43 42 18
EagI C'GGCC_G
Cuts at: 1537 1812 2693 2735 3562 1537
Size: 275 881 42 827 4090
Fragments arranged by size:
4090 881 827 275 42
EarI CTCTTCn'nnn_
Cuts at: 1353 4045 1353
Size: 2692 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2191 3055 3059 4702 5076 5222
Size: 1064 138 602 864 4 1643 374 146
Cuts at: 5222 387
Size: 1280
Fragments arranged by size:
1643 1280 1064 864 602 374 146 138
4
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2076 3130 3197 3334 4178 4775 5562
Size: 393 444 1054 67 137 844 597 787
Cuts at: 5562 5830 1239
Size: 268 1524
Fragments arranged by size:
1524 1054 844 787 597 444 393 268
137 67
EcoO109I rG'GnC_Cy
Cuts at: 2700 2701 3266 3267 2700
Size: 1 565 1 5548
Fragments arranged by size:
5548 565 1 1
EcoRI G'AATT_C
Cuts at: 3424 3802 3424
Size: 378 5737
FspI TGC'GCA
Cuts at: 2867 4621 2867
Size: 1754 4361
HaeII r_GCGC'y
Cuts at: 1678 2189 2285 2294 2492 2564 2588 2747 5262
Size: 511 96 9 198 72 24 159 2515
Cuts at: 5262 1678
Size: 2531
Fragments arranged by size:
2531 2515 511 198 159 96 72 24
9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2293 2325 2650 2682 2705
Size: 32 135 32 721 32 325 32 23
Cuts at: 2705 2737 4756 4788 4830 4862 1373
Size: 32 2019 32 42 32 2626
Fragments arranged by size:
2626 2019 721 325 135 42 32 32
32 32 32 32 32 23
HincII GTy'rAC
Cuts at: 669 1657 1938 3459 3705 669
Size: 988 281 1521 246 3079
Fragments arranged by size:
3079 1521 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6115
HpaI GTT'AAC
Cuts at: 3705 3705
Size: 6115
KasI G'GCGC_C
Cuts at: 2281 2290 2281
Size: 9 6106
KpnI G_GTAC'C
Cuts at: 3440 3440
Size: 6115
MfeI C'AATT_G
Cuts at: 3714 3714
Size: 6115
MluI A'CGCG_T
Cuts at: 5991 5991
Size: 6115
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5229 5413 1305
Size: 3924 184 2007
Fragments arranged by size:
3924 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4293
Fragments arranged by size:
4293 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2054 3251 4073 4432 4591 518
Size: 648 888 1197 822 359 159 2042
Fragments arranged by size:
2042 1197 888 822 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2199 2397 2425 2598 2783 2854 3531 4208
Size: 593 198 28 173 185 71 677 677
Cuts at: 4208 5356 5601 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 677 593 245 198 185
173 71 28
NaeI GCC'GGC
Cuts at: 3042 3042
Size: 6115
NarI GG'CG_CC
Cuts at: 2282 2291 2282
Size: 9 6106
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4624
Fragments arranged by size:
4624 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6115
NgoMIV G'CCGG_C
Cuts at: 3040 3040
Size: 6115
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6115
NotI GC'GGCC_GC
Cuts at: 3562 3562
Size: 6115
NspI r_CATG'y
Cuts at: 1132 1229 2053 2254 2953 3473 5018 1132
Size: 97 824 201 699 520 1545 2229
Fragments arranged by size:
2229 1545 824 699 520 201 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6115
PciI A'CATG_T
Cuts at: 5014 5014
Size: 6115
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2854 3108 1108
Size: 1746 254 4115
Fragments arranged by size:
4115 1746 254
PmeI GTTT'AAAC
Cuts at: 3419 3419
Size: 6115
PmlI CAC'GTG
Cuts at: 2476 2476
Size: 6115
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4184 4216 5697 5729 4184
Size: 32 1481 32 4570
Fragments arranged by size:
4570 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6115
PsiI TTA'TAA
Cuts at: 3685 3685
Size: 6115
PspOMI G'GGCC_C
Cuts at: 2700 3266 2700
Size: 566 5549
PstI C_TGCA'G
Cuts at: 830 2958 3024 3189 3467 830
Size: 2128 66 165 278 3478
Fragments arranged by size:
3478 2128 278 165 66
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6115
PvuII CAG'CTG
Cuts at: 2854 2854
Size: 6115
SacII CC_GC'GG
Cuts at: 2426 2426
Size: 6115
SalI G'TCGA_C
Cuts at: 1936 3457 1936
Size: 1521 4594
SbfI CC_TGCA'GG
Cuts at: 3467 3467
Size: 6115
ScaI AGT'ACT
Cuts at: 2816 4363 6075 2816
Size: 1547 1712 2856
Fragments arranged by size:
2856 1712 1547
SfcI C'TryA_G
Cuts at: 826 1045 2245 2443 2954 3020 3185 3463 4598
Size: 219 1200 198 511 66 165 278 1135
Cuts at: 4598 5279 5470 826
Size: 681 191 1471
Fragments arranged by size:
1471 1200 1135 681 511 278 219 198
191 165 66
SfoI GGC'GCC
Cuts at: 2283 2292 2283
Size: 9 6106
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6115
SgrAI Cr'CCGG_yG
Cuts at: 1700 3040 1700
Size: 1340 4775
SmaI CCC'GGG
Cuts at: 1872 2146 2344 3442 1872
Size: 274 198 1098 4545
Fragments arranged by size:
4545 1098 274 198
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2747 2894 3146 4219 5120 5382
Size: 197 926 804 147 252 1073 901 262
Cuts at: 5382 820
Size: 1553
Fragments arranged by size:
1553 1073 926 901 804 262 252 197
147
SnaBI TAC'GTA
Cuts at: 493 3906 493
Size: 3413 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6115
SphI G_CATG'C
Cuts at: 1132 2254 2953 3473 1132
Size: 1122 699 520 3774
Fragments arranged by size:
3774 1122 699 520
SspI AAT'ATT
Cuts at: 5 52 4039 5
Size: 47 3987 2081
Fragments arranged by size:
3987 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6115
StyI C'CwwG_G
Cuts at: 513 1065 2004 3129 3262 3554 513
Size: 552 939 1125 133 292 3074
Fragments arranged by size:
3074 1125 939 552 292 133
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2238 4142 4159 4312 4497 4942 325
Size: 1255 658 1904 17 153 185 445 1498
Fragments arranged by size:
1904 1498 1255 658 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2814 3198 3404
Size: 274 80 33 51 1443 837 384 206
Cuts at: 3404 4361 6073 96
Size: 957 1712 138
Fragments arranged by size:
1712 1443 957 837 384 274 206 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6075 6107 6075
Size: 32 6083
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6115
XbaI T'CTAG_A
Cuts at: 3451 3451
Size: 6115
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2511 2511
Size: 6115
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6115
XmaI C'CCGG_G
Cuts at: 1870 2144 2342 3440 1870
Size: 274 198 1098 4545
Fragments arranged by size:
4545 1098 274 198
XmnI GAAnn'nnTTC
Cuts at: 4244 4244
Size: 6115
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3923 276
Size: 53 83 186 936 2389 2468
Fragments arranged by size:
2468 2389 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BfrBI BstXI BstZ17I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI
NsiI PacI PfoI PpuMI PspXI PsrI RsrII SacI
SanDI SapI SexAI SfiI SrfI SwaI