Restriction map for
pFN21AB7039
(Circular) (Six-base) MAPSORT of: pFN21AB7039.seq Check: 6050 from: 1 to: 6079
pFN21AB7039 6079 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3889 278
Size: 53 83 186 936 2353 2468
Fragments arranged by size:
2468 2353 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3422 1937
Size: 1485 4594
Acc65I G'GTAC_C
Cuts at: 3400 3400
Size: 6079
AclI AA'CG_TT
Cuts at: 1563 4206 4579 1563
Size: 2643 373 3063
Fragments arranged by size:
3063 2643 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2010 4142 5526 5794 1632
Size: 378 2132 1384 268 1917
Fragments arranged by size:
2132 1917 1384 378 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5882
AflIII A'CryG_T
Cuts at: 1203 3207 4978 5955 1203
Size: 2004 1771 977 1327
Fragments arranged by size:
2004 1771 1327 977
AgeI A'CCGG_T
Cuts at: 4910 4910
Size: 6079
AhdI GACnn_n'nnGTC
Cuts at: 3123 4808 3123
Size: 1685 4394
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6079
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6079
AlwNI CAG_nnn'CTG
Cuts at: 2344 2947 3822 5394 2344
Size: 603 875 1572 3029
Fragments arranged by size:
3029 1572 875 603
ApaLI G'TGCA_C
Cuts at: 2227 4136 5292 2227
Size: 1909 1156 3014
Fragments arranged by size:
3014 1909 1156
ApoI r'AATT_y
Cuts at: 1493 3388 3614 3766 5875 1493
Size: 1895 226 152 2109 1697
Fragments arranged by size:
2109 1895 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4633 160
Size: 872 3601 1606
Fragments arranged by size:
3601 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6079
AvaI C'yCGr_G
Cuts at: 1870 1943 2765 3404 1870
Size: 73 822 639 4545
Fragments arranged by size:
4545 822 639 73
BamHI G'GATC_C
Cuts at: 3409 3776 3409
Size: 367 5712
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3400 4855 618
Size: 325 217 627 1613 1455 1842
Fragments arranged by size:
1842 1613 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2250 3398 1391
Size: 859 1148 4072
Fragments arranged by size:
4072 1148 859
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2466 928
Size: 971 567 4541
Fragments arranged by size:
4541 971 567
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4270 4304 1903
Size: 34 2333 34 3678
Fragments arranged by size:
3678 2333 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3971 5181 3971
Size: 1210 4869
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6079
BfrBI ATG'CAT
Cuts at: 2806 2806
Size: 6079
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4690 136
Size: 107 122 71 631 605 3018 1525
Fragments arranged by size:
3018 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6074 6074
Size: 6079
BlpI GC'TnA_GC
Cuts at: 2093 2249 3496 2093
Size: 156 1247 4676
Fragments arranged by size:
4676 1247 156
Bme1580I G_kGCm'C
Cuts at: 1790 2231 2766 4140 5296 1790
Size: 441 535 1374 1156 2573
Fragments arranged by size:
2573 1374 1156 535 441
BmgBI CAC'GTC
Cuts at: 1318 2872 3235 1318
Size: 1554 363 4162
Fragments arranged by size:
4162 1554 363
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3041 3810 4768
Size: 416 53 83 399 24 1624 769 958
Cuts at: 4768 442
Size: 1753
Fragments arranged by size:
1753 1624 958 769 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6079
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2086 2245 2286 2366 2765 2798 2897 4739
Size: 847 159 41 80 399 33 99 1842
Cuts at: 4739 1239
Size: 2579
Fragments arranged by size:
2579 1842 847 399 159 99 80 41
33
Bpu10I CC'TnA_GC
Cuts at: 2186 3064 2186
Size: 878 5201
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2387 4204 5069 5367 2387
Size: 1817 865 298 3099
Fragments arranged by size:
3099 1817 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2062 2660 2741 3266 4742 882
Size: 617 563 598 81 525 1476 2219
Fragments arranged by size:
2219 1476 617 598 563 525 81
BsaAI yAC'GTr
Cuts at: 493 1204 2905 3208 3870 493
Size: 711 1701 303 662 2702
Fragments arranged by size:
2702 1701 711 662 303
BsaBI GATnn'nnATC
Cuts at: 6073 6073
Size: 6079
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3886 4268 4903 275
Size: 53 83 186 936 2353 382 635 1451
Fragments arranged by size:
2353 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2197 4512 4910 5184 5331 5742 2197
Size: 2315 398 274 147 411 2534
Fragments arranged by size:
2534 2315 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2138 2168 3176 3206 1178
Size: 30 165 30 735 30 1008 30 4051
Fragments arranged by size:
4051 1008 735 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2162 2678 2795 1186
Size: 976 516 117 4470
Fragments arranged by size:
4470 976 516 117
BseYI C'CCAG_C
Cuts at: 2104 2134 2896 5282 5778 2104
Size: 30 762 2386 496 2405
Fragments arranged by size:
2405 2386 762 496 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3026 1163
Size: 573 1290 4216
Fragments arranged by size:
4216 1290 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2548 3529 4290 5318 1540
Size: 275 186 547 981 761 1028 2301
Fragments arranged by size:
2301 1028 981 761 547 275 186
BsiHKAI G_wGCw'C
Cuts at: 2169 2231 2250 4140 4225 5296 2169
Size: 62 19 1890 85 1071 2952
Fragments arranged by size:
2952 1890 1071 85 62 19
BsmI GAATG_Cn'
Cuts at: 3590 3683 3590
Size: 93 5986
BsmBI CGTCTCn'nnnn_
Cuts at: 3353 3353
Size: 6079
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2169 2231 2250 2766 3398 4140 4225
Size: 399 379 62 19 516 632 742 85
Cuts at: 4225 5296 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 632 516 399 379 85
62 19
BspEI T'CCGG_A
Cuts at: 5742 5742
Size: 6079
BspHI T'CATG_A
Cuts at: 3132 3968 5698 3132
Size: 836 1730 3513
Fragments arranged by size:
3513 1730 836
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2190 2916 3048 3434 844
Size: 1346 726 132 386 3489
Fragments arranged by size:
3489 1346 726 386 132
BsrBI CCG'CTC
Cuts at: 1394 1823 3966 1394
Size: 429 2143 3507
Fragments arranged by size:
3507 2143 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4574 4748 65
Size: 1183 3326 174 1396
Fragments arranged by size:
3326 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2921 4723 4910 1700
Size: 110 1111 1802 187 2869
Fragments arranged by size:
2869 1802 1111 187 110
BsrGI T'GTAC_A
Cuts at: 96 2490 3007 96
Size: 2394 517 3168
Fragments arranged by size:
3168 2394 517
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6079
BssSI C'ACGA_G
Cuts at: 4139 5151 4139
Size: 1012 5067
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6079
BstBI TT'CG_AA
Cuts at: 4883 4883
Size: 6079
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6079
BstYI r'GATC_y
Cuts at: 1972 2880 3409 3776 4161 4178 5630 5716 5728
Size: 908 529 367 385 17 1452 86 12
Cuts at: 5728 6074 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 908 529 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2241 2913 513
Size: 552 1176 672 3679
Fragments arranged by size:
3679 1176 672 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3241 487
Size: 683 56 2015 3325
Fragments arranged by size:
3325 2015 683 56
BtsI GCAGTG_nn'
Cuts at: 2159 2552 3320 3595 4389 4409 2159
Size: 393 768 275 794 20 3829
Fragments arranged by size:
3829 794 768 393 275 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6079
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6044
DraI TTT'AAA
Cuts at: 3383 3383
Size: 6079
DraIII CAC_nnn'GTG
Cuts at: 2226 2695 3049 2226
Size: 469 354 5256
Fragments arranged by size:
5256 469 354
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5086 809
Size: 507 3770 1802
Fragments arranged by size:
3770 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2283 2697 3526
Size: 54 1425 50 275 18 453 414 829
Cuts at: 3526 4415 6044 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 829 453 414 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3526 1537
Size: 275 1714 4090
Fragments arranged by size:
4090 1714 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2393 2562 2765 4009 1353
Size: 1040 169 203 1244 3423
Fragments arranged by size:
3423 1244 1040 203 169
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2495 4666 5040 5186 387
Size: 1064 138 906 2171 374 146 1280
Fragments arranged by size:
2171 1280 1064 906 374 146 138
EcoICRI GAG'CTC
Cuts at: 2248 2248
Size: 6079
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2010 2086 2245 2286 2366 2765 2798
Size: 393 378 76 159 41 80 399 33
Cuts at: 2798 2897 4142 4739 5526 5794 1239
Size: 99 1245 597 787 268 1524
Fragments arranged by size:
1524 1245 787 597 399 393 378 268
159 99 80 76 41 33
EcoNI CCTnn'n_nnAGG
Cuts at: 2336 3149 2336
Size: 813 5266
EcoO109I rG'GnC_Cy
Cuts at: 2109 2341 2944 3117 2109
Size: 232 603 173 5071
Fragments arranged by size:
5071 603 232 173
EcoRI G'AATT_C
Cuts at: 3388 3766 3388
Size: 378 5701
FspI TGC'GCA
Cuts at: 2651 4585 2651
Size: 1934 4145
HaeII r_GCGC'y
Cuts at: 1678 2359 5226 1678
Size: 681 2867 2531
Fragments arranged by size:
2867 2531 681
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2542 2574 2946 2978 3109
Size: 32 135 32 970 32 372 32 131
Cuts at: 3109 3141 3176 3208 4720 4752 4794 4826 1373
Size: 32 35 32 1512 32 42 32 2626
Fragments arranged by size:
2626 1512 970 372 135 131 42 35
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2539 3423 3669 669
Size: 988 281 601 884 246 3079
Fragments arranged by size:
3079 988 884 601 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6079
HpaI GTT'AAC
Cuts at: 3669 3669
Size: 6079
KpnI G_GTAC'C
Cuts at: 3404 3404
Size: 6079
MfeI C'AATT_G
Cuts at: 3678 3678
Size: 6079
MluI A'CGCG_T
Cuts at: 5955 5955
Size: 6079
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2295 2932 3192 3219 5193 5377 1305
Size: 990 637 260 27 1974 184 2007
Fragments arranged by size:
2007 1974 990 637 260 184 27
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2285 2699 10
Size: 54 1425 343 453 414 3390
Fragments arranged by size:
3390 1425 453 414 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2018 2801 3146 4037 4396 4555 518
Size: 648 852 783 345 891 359 159 2042
Fragments arranged by size:
2042 891 852 783 648 359 345 159
MspA1I CmG'CkG
Cuts at: 1606 2134 2306 3190 3495 4172 5320 5565 1606
Size: 528 172 884 305 677 1148 245 2120
Fragments arranged by size:
2120 1148 884 677 528 305 245 172
NaeI GCC'GGC
Cuts at: 2923 2923
Size: 6079
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5527
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6079
NgoMIV G'CCGG_C
Cuts at: 2921 2921
Size: 6079
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6079
NotI GC'GGCC_GC
Cuts at: 3526 3526
Size: 6079
NsiI A_TGCA'T
Cuts at: 2808 2808
Size: 6079
NspI r_CATG'y
Cuts at: 1132 1229 2216 2806 2810 3437 4982 1132
Size: 97 987 590 4 627 1545 2229
Fragments arranged by size:
2229 1545 987 627 590 97 4
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6079
PciI A'CATG_T
Cuts at: 4978 4978
Size: 6079
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2344 2695 3211 1108
Size: 1236 351 516 3976
Fragments arranged by size:
3976 1236 516 351
PfoI T'CCnGG_A
Cuts at: 2742 2742
Size: 6079
PmeI GTTT'AAAC
Cuts at: 3383 3383
Size: 6079
PmlI CAC'GTG
Cuts at: 3208 3208
Size: 6079
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4148 4180 5661 5693 4148
Size: 32 1481 32 4534
Fragments arranged by size:
4534 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2109 2944 3117 2109
Size: 835 173 5071
Fragments arranged by size:
5071 835 173
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6079
PsiI TTA'TAA
Cuts at: 3075 3649 3075
Size: 574 5505
PstI C_TGCA'G
Cuts at: 830 3431 830
Size: 2601 3478
PvuI CG_AT'CG
Cuts at: 2001 2548 2001
Size: 547 5532
RsrII CG'GwC_CG
Cuts at: 3192 3192
Size: 6079
SacI G_AGCT'C
Cuts at: 2250 2250
Size: 6079
SalI G'TCGA_C
Cuts at: 1936 3421 1936
Size: 1485 4594
SanDI GG'GwC_CC
Cuts at: 2109 2109
Size: 6079
SapI GCTCTTCn'nnn_
Cuts at: 2393 2765 2393
Size: 372 5707
SbfI CC_TGCA'GG
Cuts at: 3431 3431
Size: 6079
ScaI AGT'ACT
Cuts at: 3311 4327 6039 3311
Size: 1016 1712 3351
Fragments arranged by size:
3351 1712 1016
SfcI C'TryA_G
Cuts at: 826 1045 3427 4562 5243 5434 826
Size: 219 2382 1135 681 191 1471
Fragments arranged by size:
2382 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6079
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6079
SmaI CCC'GGG
Cuts at: 1872 2767 3406 1872
Size: 895 639 4545
Fragments arranged by size:
4545 895 639
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2402 4183 5084 5346 820
Size: 197 926 459 1781 901 262 1553
Fragments arranged by size:
1781 1553 926 901 459 262 197
SnaBI TAC'GTA
Cuts at: 493 3870 493
Size: 3377 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6079
SphI G_CATG'C
Cuts at: 1132 2216 2806 2810 3437 1132
Size: 1084 590 4 627 3774
Fragments arranged by size:
3774 1084 627 590 4
SspI AAT'ATT
Cuts at: 5 52 4003 5
Size: 47 3951 2081
Fragments arranged by size:
3951 2081 47
StuI AGG'CCT
Cuts at: 1517 2175 2297 3241 1517
Size: 658 122 944 4355
Fragments arranged by size:
4355 944 658 122
StyI C'CwwG_G
Cuts at: 513 1065 2292 2631 3518 513
Size: 552 1227 339 887 3074
Fragments arranged by size:
3074 1227 887 552 339
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2050 2071 3060 4106 4123 4276 4461
Size: 1255 470 21 989 1046 17 153 185
Cuts at: 4461 4906 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1046 989 470 445 185 153
21 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2490 2820 3007
Size: 274 80 33 51 1443 513 330 187
Cuts at: 3007 3266 3309 4325 6037 96
Size: 259 43 1016 1712 138
Fragments arranged by size:
1712 1443 1016 513 330 274 259 187
138 80 51 43 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2138 2170 6039 6071 2138
Size: 32 3869 32 2146
Fragments arranged by size:
3869 2146 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6079
XbaI T'CTAG_A
Cuts at: 3415 3415
Size: 6079
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6079
XmaI C'CCGG_G
Cuts at: 1870 2765 3404 1870
Size: 895 639 4545
Fragments arranged by size:
4545 895 639
XmnI GAAnn'nnTTC
Cuts at: 4208 4208
Size: 6079
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3887 276
Size: 53 83 186 936 2353 2468
Fragments arranged by size:
2468 2353 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BstXI BstZ17I Bsu36I EcoRV FalI
FseI FspAI KasI NarI NruI PacI PspOMI PspXI
PsrI PvuII SacII SexAI SfiI SfoI SrfI SwaI
XcmI