Restriction map for
pFN21AB7036
(Circular) (Six-base) MAPSORT of: pFN21AB7036.seq Check: 9932 from: 1 to: 5896
pFN21AB7036 5896 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3706 278
Size: 53 83 186 936 2170 2468
Fragments arranged by size:
2468 2170 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2547 3239 1937
Size: 610 692 4594
Fragments arranged by size:
4594 692 610
Acc65I G'GTAC_C
Cuts at: 2379 2898 3217 2379
Size: 519 319 5058
Fragments arranged by size:
5058 519 319
AclI AA'CG_TT
Cuts at: 1563 4023 4396 1563
Size: 2460 373 3063
Fragments arranged by size:
3063 2460 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3044 3959 5343 5611 1632
Size: 1412 915 1384 268 1917
Fragments arranged by size:
1917 1412 1384 915 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5699
AflIII A'CryG_T
Cuts at: 1203 4795 5772 1203
Size: 3592 977 1327
Fragments arranged by size:
3592 1327 977
AgeI A'CCGG_T
Cuts at: 2349 4727 2349
Size: 2378 3518
AhdI GACnn_n'nnGTC
Cuts at: 4625 4625
Size: 5896
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5896
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5896
AlwNI CAG_nnn'CTG
Cuts at: 3639 5211 3639
Size: 1572 4324
ApaLI G'TGCA_C
Cuts at: 3953 5109 3953
Size: 1156 4740
ApoI r'AATT_y
Cuts at: 1493 3205 3431 3583 5692 1493
Size: 1712 226 152 2109 1697
Fragments arranged by size:
2109 1712 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4450 160
Size: 872 3418 1606
Fragments arranged by size:
3418 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5896
AvaI C'yCGr_G
Cuts at: 1870 1943 2631 3221 1870
Size: 73 688 590 4545
Fragments arranged by size:
4545 688 590 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2363 2396 2363
Size: 33 5863
BamHI G'GATC_C
Cuts at: 3226 3593 3226
Size: 367 5529
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2379 2898 3217 4672
Size: 325 217 627 221 371 519 319 1455
Cuts at: 4672 618
Size: 1842
Fragments arranged by size:
1842 1455 627 519 371 325 319 221
217
BanII G_rGCy'C
Cuts at: 1391 3215 1391
Size: 1824 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2263 928
Size: 971 364 4561
Fragments arranged by size:
4561 971 364
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2098 2132 4087 4121 1903
Size: 34 161 34 1955 34 3678
Fragments arranged by size:
3678 1955 161 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3788 4998 3788
Size: 1210 4686
BclI T'GATC_A
Cuts at: 1548 2337 2592 1548
Size: 789 255 4852
Fragments arranged by size:
4852 789 255
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4507 136
Size: 107 122 71 631 605 2835 1525
Fragments arranged by size:
2835 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5891 5891
Size: 5896
BlpI GC'TnA_GC
Cuts at: 3313 3313
Size: 5896
Bme1580I G_kGCm'C
Cuts at: 1790 3957 5113 1790
Size: 2167 1156 2573
Fragments arranged by size:
2573 2167 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5896
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2393 3627 4585
Size: 416 53 83 399 24 976 1234 958
Cuts at: 4585 442
Size: 1753
Fragments arranged by size:
1753 1234 976 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5896
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3014 4556 1239
Size: 1775 1542 2579
Fragments arranged by size:
2579 1775 1542
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4021 4886 5184 4021
Size: 865 298 4733
Fragments arranged by size:
4733 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2486 4559 882
Size: 617 987 2073 2219
Fragments arranged by size:
2219 2073 987 617
BsaAI yAC'GTr
Cuts at: 493 1204 3687 493
Size: 711 2483 2702
Fragments arranged by size:
2702 2483 711
BsaBI GATnn'nnATC
Cuts at: 2063 5890 2063
Size: 3827 2069
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3703 4085 4720 275
Size: 53 83 186 936 2170 382 635 1451
Fragments arranged by size:
2170 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2349 4329 4727 5001 5148 5559 2349
Size: 1980 398 274 147 411 2686
Fragments arranged by size:
2686 1980 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2648 2678 2921 2951 1178
Size: 30 165 30 1245 30 243 30 4123
Fragments arranged by size:
4123 1245 243 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2192 1186
Size: 1006 4890
BseYI C'CCAG_C
Cuts at: 2830 5099 5595 2830
Size: 2269 496 3131
Fragments arranged by size:
3131 2269 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2293 1163
Size: 573 557 4766
Fragments arranged by size:
4766 573 557
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2350 3346 4107 5135 1540
Size: 275 186 349 996 761 1028 2301
Fragments arranged by size:
2301 1028 996 761 349 275 186
BsiHKAI G_wGCw'C
Cuts at: 2694 3957 4042 5113 2694
Size: 1263 85 1071 3477
Fragments arranged by size:
3477 1263 1071 85
BsmI GAATG_Cn'
Cuts at: 3107 3407 3500 3107
Size: 300 93 5503
Fragments arranged by size:
5503 300 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2694 3215 3957 4042 5113 1391
Size: 399 904 521 742 85 1071 2174
Fragments arranged by size:
2174 1071 904 742 521 399 85
BspEI T'CCGG_A
Cuts at: 5559 5559
Size: 5896
BspHI T'CATG_A
Cuts at: 2334 3785 5515 2334
Size: 1451 1730 2715
Fragments arranged by size:
2715 1730 1451
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2205 3251 844
Size: 1361 1046 3489
Fragments arranged by size:
3489 1361 1046
BsrBI CCG'CTC
Cuts at: 1394 1823 3783 1394
Size: 429 1960 3507
Fragments arranged by size:
3507 1960 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2116 4391 4565 65
Size: 1183 868 2275 174 1396
Fragments arranged by size:
2275 1396 1183 868 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2145 2349 4540 4727 1700
Size: 110 335 204 2191 187 2869
Fragments arranged by size:
2869 2191 335 204 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5896
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5896
BssSI C'ACGA_G
Cuts at: 3956 4968 3956
Size: 1012 4884
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5896
BstBI TT'CG_AA
Cuts at: 4700 4700
Size: 5896
BstEII G'GTnAC_C
Cuts at: 1184 2392 2569 1184
Size: 1208 177 4511
Fragments arranged by size:
4511 1208 177
BstXI CCAn_nnnn'nTGG
Cuts at: 2071 2419 3105 2071
Size: 348 686 4862
Fragments arranged by size:
4862 686 348
BstYI r'GATC_y
Cuts at: 1972 3226 3593 3978 3995 5447 5533 5545 5891
Size: 1254 367 385 17 1452 86 12 346
Cuts at: 5891 1972
Size: 1977
Fragments arranged by size:
1977 1452 1254 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2548 2548
Size: 5896
BtgI C'CryG_G
Cuts at: 513 1065 2004 2070 2130 2773 3083 513
Size: 552 939 66 60 643 310 3326
Fragments arranged by size:
3326 939 643 552 310 66 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5157
Fragments arranged by size:
5157 683 56
BtsI GCAGTG_nn'
Cuts at: 2372 3412 4206 4226 2372
Size: 1040 794 20 4042
Fragments arranged by size:
4042 1040 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5896
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5861
DraI TTT'AAA
Cuts at: 3200 3200
Size: 5896
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4903 809
Size: 507 3587 1802
Fragments arranged by size:
3587 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2127 2712 2925
Size: 54 1425 50 275 18 297 585 213
Cuts at: 2925 3343 4232 5861 8
Size: 418 889 1629 43
Fragments arranged by size:
1629 1425 889 585 418 297 275 213
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3343 1537
Size: 275 1531 4090
Fragments arranged by size:
4090 1531 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2032 2336 2915 3006 3826 1353
Size: 679 304 579 91 820 3423
Fragments arranged by size:
3423 820 679 579 304 91
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4483 4857 5003 387
Size: 1064 138 2894 374 146 1280
Fragments arranged by size:
2894 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3014 3044 3959 4556 5343 5611 1239
Size: 393 1382 30 915 597 787 268 1524
Fragments arranged by size:
1524 1382 915 787 597 393 268 30
EcoO109I rG'GnC_Cy
Cuts at: 2727 2982 2727
Size: 255 5641
EcoRI G'AATT_C
Cuts at: 3205 3583 3205
Size: 378 5518
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2275 2275
Size: 5896
FspI TGC'GCA
Cuts at: 4402 4402
Size: 5896
HaeII r_GCGC'y
Cuts at: 1678 5043 1678
Size: 3365 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2638 2670 4537 4569 4611
Size: 32 135 32 1066 32 1867 32 42
Cuts at: 4611 4643 1373
Size: 32 2626
Fragments arranged by size:
2626 1867 1066 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3240 3486 669
Size: 988 281 1302 246 3079
Fragments arranged by size:
3079 1302 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5896
HpaI GTT'AAC
Cuts at: 3486 3486
Size: 5896
KpnI G_GTAC'C
Cuts at: 2383 2902 3221 2383
Size: 519 319 5058
Fragments arranged by size:
5058 519 319
MfeI C'AATT_G
Cuts at: 2164 3495 2164
Size: 1331 4565
MluI A'CGCG_T
Cuts at: 5772 5772
Size: 5896
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2985 5010 5194 1305
Size: 1680 2025 184 2007
Fragments arranged by size:
2025 2007 1680 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2927 10
Size: 54 1425 343 1095 2979
Fragments arranged by size:
2979 1425 1095 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2069 3854 4213 4372 518
Size: 648 903 1785 359 159 2042
Fragments arranged by size:
2042 1785 903 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2242 2974 3312 3989 5137 5382 1606
Size: 636 732 338 677 1148 245 2120
Fragments arranged by size:
2120 1148 732 677 636 338 245
NaeI GCC'GGC
Cuts at: 2147 2147
Size: 5896
NcoI C'CATG_G
Cuts at: 513 1065 2004 3083 513
Size: 552 939 1079 3326
Fragments arranged by size:
3326 1079 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5896
NgoMIV G'CCGG_C
Cuts at: 2145 2145
Size: 5896
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5896
NotI GC'GGCC_GC
Cuts at: 3343 3343
Size: 5896
NspI r_CATG'y
Cuts at: 1132 1229 2055 3186 3254 4799 1132
Size: 97 826 1131 68 1545 2229
Fragments arranged by size:
2229 1545 1131 826 97 68
PasI CC'CwG_GG
Cuts at: 1348 3064 1348
Size: 1716 4180
PciI A'CATG_T
Cuts at: 4795 4795
Size: 5896
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5896
PfoI T'CCnGG_A
Cuts at: 2666 2978 2666
Size: 312 5584
PmeI GTTT'AAAC
Cuts at: 3200 3200
Size: 5896
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3965 3997 5478 5510 3965
Size: 32 1481 32 4351
Fragments arranged by size:
4351 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2982 2982
Size: 5896
PshAI GACnn'nnGTC
Cuts at: 1613 3050 1613
Size: 1437 4459
PsiI TTA'TAA
Cuts at: 3466 3466
Size: 5896
PstI C_TGCA'G
Cuts at: 830 2921 3248 830
Size: 2091 327 3478
Fragments arranged by size:
3478 2091 327
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5896
PvuII CAG'CTG
Cuts at: 2242 2974 2242
Size: 732 5164
SalI G'TCGA_C
Cuts at: 1936 3238 1936
Size: 1302 4594
SapI GCTCTTCn'nnn_
Cuts at: 2915 2915
Size: 5896
SbfI CC_TGCA'GG
Cuts at: 3248 3248
Size: 5896
ScaI AGT'ACT
Cuts at: 4144 5856 4144
Size: 1712 4184
SfcI C'TryA_G
Cuts at: 826 1045 2917 3244 4379 5060 5251 826
Size: 219 1872 327 1135 681 191 1471
Fragments arranged by size:
1872 1471 1135 681 327 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5896
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5896
SmaI CCC'GGG
Cuts at: 1872 2633 3223 1872
Size: 761 590 4545
Fragments arranged by size:
4545 761 590
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4000 4901 5163 820
Size: 197 926 2057 901 262 1553
Fragments arranged by size:
2057 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3687 493
Size: 3194 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5896
SphI G_CATG'C
Cuts at: 1132 2055 3254 1132
Size: 923 1199 3774
Fragments arranged by size:
3774 1199 923
SspI AAT'ATT
Cuts at: 5 52 3820 5
Size: 47 3768 2081
Fragments arranged by size:
3768 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5896
StyI C'CwwG_G
Cuts at: 513 1065 2004 2181 3083 3335 513
Size: 552 939 177 902 252 3074
Fragments arranged by size:
3074 939 902 552 252 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2026 3923 3940 4093 4278 4723 325
Size: 1255 446 1897 17 153 185 445 1498
Fragments arranged by size:
1897 1498 1255 446 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4142 5854 96
Size: 274 80 33 51 1443 2165 1712 138
Fragments arranged by size:
2165 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2648 2680 5856 5888 2648
Size: 32 3176 32 2656
Fragments arranged by size:
3176 2656 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5896
XbaI T'CTAG_A
Cuts at: 3232 3232
Size: 5896
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2707 3038 2707
Size: 331 5565
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5896
XmaI C'CCGG_G
Cuts at: 1870 2631 3221 1870
Size: 761 590 4545
Fragments arranged by size:
4545 761 590
XmnI GAAnn'nnTTC
Cuts at: 4025 4025
Size: 5896
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3704 276
Size: 53 83 186 936 2170 2468
Fragments arranged by size:
2468 2170 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI