Restriction map for
pFN21AB7034
(Circular) (Six-base) MAPSORT of: pFN21AB7034.seq Check: 6208 from: 1 to: 5980
pFN21AB7034 5980 bp
With 182 enzymes: SgfI *
November 11, 2016 17:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3790 278
Size: 53 83 186 936 2254 2468
Fragments arranged by size:
2468 2254 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3150 3323 1937
Size: 1213 173 4594
Fragments arranged by size:
4594 1213 173
Acc65I G'GTAC_C
Cuts at: 3301 3301
Size: 5980
AclI AA'CG_TT
Cuts at: 1563 4107 4480 1563
Size: 2544 373 3063
Fragments arranged by size:
3063 2544 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2026 4043 5427 5695 1632
Size: 394 2017 1384 268 1917
Fragments arranged by size:
2017 1917 1384 394 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5783
AflIII A'CryG_T
Cuts at: 1203 2559 4879 5856 1203
Size: 1356 2320 977 1327
Fragments arranged by size:
2320 1356 1327 977
AgeI A'CCGG_T
Cuts at: 2601 4811 2601
Size: 2210 3770
AhdI GACnn_n'nnGTC
Cuts at: 4709 4709
Size: 5980
AleI CACnn'nnGTG
Cuts at: 1166 2336 1166
Size: 1170 4810
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5980
AlwNI CAG_nnn'CTG
Cuts at: 2301 2954 3047 3723 5295 2301
Size: 653 93 676 1572 2986
Fragments arranged by size:
2986 1572 676 653 93
ApaI G_GGCC'C
Cuts at: 2083 2083
Size: 5980
ApaLI G'TGCA_C
Cuts at: 4037 5193 4037
Size: 1156 4824
ApoI r'AATT_y
Cuts at: 1493 3289 3515 3667 5776 1493
Size: 1796 226 152 2109 1697
Fragments arranged by size:
2109 1796 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4534 160
Size: 872 3502 1606
Fragments arranged by size:
3502 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
AvaI C'yCGr_G
Cuts at: 1870 1943 2076 2744 2931 3305 1870
Size: 73 133 668 187 374 4545
Fragments arranged by size:
4545 668 374 187 133 73
BamHI G'GATC_C
Cuts at: 3310 3677 3310
Size: 367 5613
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2740 3301 4756 618
Size: 325 217 627 953 561 1455 1842
Fragments arranged by size:
1842 1455 953 627 561 325 217
BanII G_rGCy'C
Cuts at: 1391 2083 2751 3299 1391
Size: 692 668 548 4072
Fragments arranged by size:
4072 692 668 548
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2827 928
Size: 971 928 4081
Fragments arranged by size:
4081 971 928
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4171 4205 1903
Size: 34 2234 34 3678
Fragments arranged by size:
3678 2234 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2631 3223 3872 5082 2631
Size: 592 649 1210 3529
Fragments arranged by size:
3529 1210 649 592
BclI T'GATC_A
Cuts at: 1548 2853 1548
Size: 1305 4675
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2017 2684 4591
Size: 107 122 71 631 605 345 667 1907
Cuts at: 4591 136
Size: 1525
Fragments arranged by size:
1907 1525 667 631 605 345 122 107
71
BglII A'GATC_T
Cuts at: 5975 5975
Size: 5980
BlpI GC'TnA_GC
Cuts at: 3397 3397
Size: 5980
Bme1580I G_kGCm'C
Cuts at: 1790 2083 2745 4041 5197 1790
Size: 293 662 1296 1156 2573
Fragments arranged by size:
2573 1296 1156 662 293
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5980
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3711 4669 442
Size: 416 53 83 399 24 2294 958 1753
Fragments arranged by size:
2294 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5980
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4640 1239
Size: 3401 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2705 4105 4970 5268 2705
Size: 1400 865 298 3417
Fragments arranged by size:
3417 1400 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4643 882
Size: 617 3144 2219
Fragments arranged by size:
3144 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3771 493
Size: 711 2567 2702
Fragments arranged by size:
2702 2567 711
BsaBI GATnn'nnATC
Cuts at: 5974 5974
Size: 5980
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3787 4169 4804 275
Size: 53 83 186 936 2254 382 635 1451
Fragments arranged by size:
2254 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2282 2333 2601 4413 4811 5085 5232 5643 2282
Size: 51 268 1812 398 274 147 411 2619
Fragments arranged by size:
2619 1812 411 398 274 268 147 51
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2328 2358 1178
Size: 30 165 30 925 30 4800
Fragments arranged by size:
4800 925 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2195 2708 3056 1186
Size: 1009 513 348 4110
Fragments arranged by size:
4110 1009 513 348
BseYI C'CCAG_C
Cuts at: 3185 5183 5679 3185
Size: 1998 496 3486
Fragments arranged by size:
3486 1998 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2414 1163
Size: 573 678 4729
Fragments arranged by size:
4729 678 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3430 4191 5219 1540
Size: 275 186 1429 761 1028 2301
Fragments arranged by size:
2301 1429 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2469 2778 4041 4126 5197 2469
Size: 309 1263 85 1071 3252
Fragments arranged by size:
3252 1263 1071 309 85
BsmI GAATG_Cn'
Cuts at: 2261 3491 3584 2261
Size: 1230 93 4657
Fragments arranged by size:
4657 1230 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2083 2469 2745 2751 2778 3299 4041
Size: 399 293 386 276 6 27 521 742
Cuts at: 4041 4126 5197 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 521 399 386 293 276
85 27 6
BspEI T'CCGG_A
Cuts at: 2282 5643 2282
Size: 3361 2619
BspHI T'CATG_A
Cuts at: 2580 2767 3869 5599 2580
Size: 187 1102 1730 2961
Fragments arranged by size:
2961 1730 1102 187
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3242 3335 844
Size: 2398 93 3489
Fragments arranged by size:
3489 2398 93
BsrBI CCG'CTC
Cuts at: 1394 1823 3867 1394
Size: 429 2044 3507
Fragments arranged by size:
3507 2044 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4475 4649 65
Size: 1183 3227 174 1396
Fragments arranged by size:
3227 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2410 2601 4624 4811 1700
Size: 110 600 191 2023 187 2869
Fragments arranged by size:
2869 2023 600 191 187 110
BsrGI T'GTAC_A
Cuts at: 96 2328 2421 96
Size: 2232 93 3655
Fragments arranged by size:
3655 2232 93
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5980
BssSI C'ACGA_G
Cuts at: 4040 5052 4040
Size: 1012 4968
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2108 3093 794
Size: 1314 985 3681
Fragments arranged by size:
3681 1314 985
BstBI TT'CG_AA
Cuts at: 4784 4784
Size: 5980
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5980
BstYI r'GATC_y
Cuts at: 1972 2119 3310 3677 4062 4079 5531 5617 5629
Size: 147 1191 367 385 17 1452 86 12
Cuts at: 5629 5975 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1191 385 367 346 147 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2012 2439 2862 513
Size: 552 939 8 427 423 3631
Fragments arranged by size:
3631 939 552 427 423 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2680 487
Size: 683 56 1454 3787
Fragments arranged by size:
3787 1454 683 56
BtsI GCAGTG_nn'
Cuts at: 2506 2538 3208 3496 4290 4310 2506
Size: 32 670 288 794 20 4176
Fragments arranged by size:
4176 794 670 288 32 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5980
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5945
DraI TTT'AAA
Cuts at: 3284 3284
Size: 5980
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4987 809
Size: 507 3671 1802
Fragments arranged by size:
3671 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2031 2370 2436
Size: 54 1425 50 275 18 201 339 66
Cuts at: 2436 2676 2691 3427 4316 5945 8
Size: 240 15 736 889 1629 43
Fragments arranged by size:
1629 1425 889 736 339 275 240 201
66 54 50 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 3427 1537
Size: 275 1615 4090
Fragments arranged by size:
4090 1615 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2247 2905 3910 1353
Size: 894 658 1005 3423
Fragments arranged by size:
3423 1005 894 658
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4567 4941 5087 387
Size: 1064 138 2978 374 146 1280
Fragments arranged by size:
2978 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2026 4043 4640 5427 5695 1239
Size: 393 394 2017 597 787 268 1524
Fragments arranged by size:
2017 1524 787 597 394 393 268
EcoRI G'AATT_C
Cuts at: 3289 3667 3289
Size: 378 5602
FspI TGC'GCA
Cuts at: 4486 4486
Size: 5980
HaeII r_GCGC'y
Cuts at: 1678 5127 1678
Size: 3449 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2111 2143 3221 3253 4621
Size: 32 135 32 539 32 1078 32 1368
Cuts at: 4621 4653 4695 4727 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1368 1078 539 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2202 3276 3324 3570 669
Size: 988 281 264 1074 48 246 3079
Fragments arranged by size:
3079 1074 988 281 264 246 48
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5980
HpaI GTT'AAC
Cuts at: 2202 3276 3570 2202
Size: 1074 294 4612
Fragments arranged by size:
4612 1074 294
KpnI G_GTAC'C
Cuts at: 3305 3305
Size: 5980
MfeI C'AATT_G
Cuts at: 3579 3579
Size: 5980
MluI A'CGCG_T
Cuts at: 5856 5856
Size: 5980
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5094 5278 1305
Size: 3789 184 2007
Fragments arranged by size:
3789 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2372 2438 2678 2693 10
Size: 54 1425 343 540 66 240 15 3297
Fragments arranged by size:
3297 1425 540 343 240 66 54 15
MslI CAynn'nnrTG
Cuts at: 518 1166 2336 2378 2738 3938 4297 4456 518
Size: 648 1170 42 360 1200 359 159 2042
Fragments arranged by size:
2042 1200 1170 648 360 359 159 42
MspA1I CmG'CkG
Cuts at: 1606 2014 2059 2851 2951 3396 4073 5221 5466
Size: 408 45 792 100 445 677 1148 245
Cuts at: 5466 1606
Size: 2120
Fragments arranged by size:
2120 1148 792 677 445 408 245 100
45
NaeI GCC'GGC
Cuts at: 2412 2412
Size: 5980
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4489
Fragments arranged by size:
4489 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5980
NgoMIV G'CCGG_C
Cuts at: 2410 2410
Size: 5980
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5980
NotI GC'GGCC_GC
Cuts at: 3427 3427
Size: 5980
NspI r_CATG'y
Cuts at: 1132 1229 2428 2563 3338 4883 1132
Size: 97 1199 135 775 1545 2229
Fragments arranged by size:
2229 1545 1199 775 135 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5980
PciI A'CATG_T
Cuts at: 2559 4879 2559
Size: 2320 3660
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2301 1108
Size: 1193 4787
PmeI GTTT'AAAC
Cuts at: 3284 3284
Size: 5980
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4049 4081 5562 5594 4049
Size: 32 1481 32 4435
Fragments arranged by size:
4435 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5980
PsiI TTA'TAA
Cuts at: 3195 3550 3195
Size: 355 5625
PspOMI G'GGCC_C
Cuts at: 2079 2079
Size: 5980
PstI C_TGCA'G
Cuts at: 830 2545 3332 830
Size: 1715 787 3478
Fragments arranged by size:
3478 1715 787
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5980
PvuII CAG'CTG
Cuts at: 2951 2951
Size: 5980
SacII CC_GC'GG
Cuts at: 2015 2015
Size: 5980
SalI G'TCGA_C
Cuts at: 1936 3322 1936
Size: 1386 4594
SapI GCTCTTCn'nnn_
Cuts at: 2905 2905
Size: 5980
SbfI CC_TGCA'GG
Cuts at: 3332 3332
Size: 5980
ScaI AGT'ACT
Cuts at: 4228 5940 4228
Size: 1712 4268
SfcI C'TryA_G
Cuts at: 826 1045 2541 3151 3328 4463 5144 5335 826
Size: 219 1496 610 177 1135 681 191 1471
Fragments arranged by size:
1496 1471 1135 681 610 219 191 177
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5980
SmaI CCC'GGG
Cuts at: 1872 2078 2746 2933 3307 1872
Size: 206 668 187 374 4545
Fragments arranged by size:
4545 668 374 206 187
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2720 4084 4985 5247 820
Size: 197 926 777 1364 901 262 1553
Fragments arranged by size:
1553 1364 926 901 777 262 197
SnaBI TAC'GTA
Cuts at: 493 3771 493
Size: 3278 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5980
SphI G_CATG'C
Cuts at: 1132 3338 1132
Size: 2206 3774
SrfI GCCC'GGGC
Cuts at: 2746 2746
Size: 5980
SspI AAT'ATT
Cuts at: 5 52 3904 5
Size: 47 3852 2081
Fragments arranged by size:
3852 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5980
StyI C'CwwG_G
Cuts at: 513 1065 2004 3419 513
Size: 552 939 1415 3074
Fragments arranged by size:
3074 1415 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4007 4024 4177 4362 4807 325
Size: 1255 2427 17 153 185 445 1498
Fragments arranged by size:
2427 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2328 2421 4226
Size: 274 80 33 51 1443 351 93 1805
Cuts at: 4226 5938 96
Size: 1712 138
Fragments arranged by size:
1805 1712 1443 351 274 138 93 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2104 2136 2301 2333 5940 5972 2104
Size: 32 165 32 3607 32 2112
Fragments arranged by size:
3607 2112 165 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5980
XbaI T'CTAG_A
Cuts at: 3316 3316
Size: 5980
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2686 2686
Size: 5980
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5980
XmaI C'CCGG_G
Cuts at: 1870 2076 2744 2931 3305 1870
Size: 206 668 187 374 4545
Fragments arranged by size:
4545 668 374 206 187
XmnI GAAnn'nnTTC
Cuts at: 2659 4109 2659
Size: 1450 4530
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3788 276
Size: 53 83 186 936 2254 2468
Fragments arranged by size:
2468 2254 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PpuMI
PspXI PsrI RsrII SacI SanDI SexAI SfiI SfoI
SwaI